(M-CP) Agar Base 500g
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Thermo ScientificTM OxoidTM
- Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples.
- Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.
Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition
| Typical Formula * |
gm/litre
|
| Tryptose |
30.0 |
| Yeast extract |
20.0 |
| Sucrose |
5.0 |
| L-cysteine hydrochloride |
1.0 |
| Magnesium sulphate.7H2O |
0.1 |
| Agar |
15.0 |
| Bromocresol purple |
0.04 |
| pH 7.6 ± 0.2 @ 25°C |
(M-CP) Agar Base, Oxoid Preparation:
Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:
| Component | Solution Strength | Volume (ml) |
| Phenolphthalein biphosphate tetrasodium salt |
0.5% |
10.0 |
| Ferric chloride hexahydrate |
4.5% |
1.0 |
| Indoxyl b-D-glucoside |
0.75% |
4.0 |
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to 5 days at 2-8°C in the dark.
(Ref: 20-188) RIPA Lysis Buffer, 10X, 100ml
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Merck
Description
100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.
Key applications:
– immunoprecipitation
– western blotting
1 kb DNA Ladder, NEB
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New England Biolabs
This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.
- Size range: 500 bp to 10 kb
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
1 kb Plus DNA Ladder, NEB
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New England Biolabs
This 1kb plus DNA ladder, NEB was previously named the 2-Log DNA Ladder. There have been no changes to product formulation or specifications. This product is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
A single convenient ladder for 100 bp up to 10 kb (100 bp and 1 kb ladder in one!)
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
1-Butanol for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 71-36-3
Chemical Formula: CH₃(CH₂)₃OH
Molar Mass: 74.12 g/mol
Synonyms: Butan-1-ol, n-Butyl alcohol, BuOH
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter
1-Propanol for analysis EMSURE® ACS,Reag. Ph Eur (Merck)
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Merck
Description
CAS number: 71-23-8
Chemical Formula: CH₃CH₂CH₂OH
Molar Mass: 60.1 g/mol
Synonyms: n-Propyl alcohol, Propan-1-ol
EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.
Product Specification and MSDS for 1-Propanol EMSURE® (Merck).
1-Propanol for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 71-23-8
Chemical Formula: CH₃CH₂CH₂OH
Molar Mass: 60.1 g/mol
Synonyms: n-Propyl alcohol, Propan-1-ol
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter.
1,2-Butanediol purum, ≥98.0% (GC) (Sigma)
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Sigma
Description
Grade: Purum
Assay: ≥98.0% (GC)
Synonym: 1,2-Butylene glycol
1,2-Butanediol has been used:
100 bp DNA Ladder, NEB
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New England Biolabs
This product is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
- Size range: 100 bp – 1,517 bp
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy-to-identify reference bands
- Value pricing
- Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes
1M-Cp Selective Supplement
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Thermo ScientificTM OxoidTM
Oxoid Membrane Clostridium Perfringens Selective Supplement (m-CP) is a chromogenic medium for rapid and presumptive identification of Clostridium perfringens from water samples.
- Add to Membrane Clostridium Perfringens (m-CP) Agar Base, Part No. CM0992B
- Each vial of SR0188E supplements 500mL of medium
1M-Cp Selective Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Polymyxin B sulphate |
12.5mg |
25.0mg |
| D-Cycloserine |
200.0mg |
400.0mg |
1M-Cp Selective Supplement, Oxoid Preparation:
Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:
| Component | Solution Strength | Volume (ml) |
| Phenolphthalein biphosphate tetrasodium salt |
0.5% |
10.0 |
| Ferric chloride hexahydrate |
4.5% |
1.0 |
| Indoxyl b-D-glucoside |
0.75% |
4.0 |
Storage conditions and Shelf life
m-CP Selective Supplement SR0188 should be stored at 2-8°C.
Store the prepared medium at 2-8°C.
1X and 10X TBE buffer (Tris-Borate-EDTA), Biobasic
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
TBE buffer (Tris-Borate-EDTA, Biobasic) is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments; whereas, TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. BIO BASIC INC. offers TBE in a number of different formats. 10X TBE liquid concentrate can be used to easily prepare a 1X TBE working solution by diluting with distilled, deionized water. 10X TBE Powder allows for the stable storage of the pre-mixed powder form that can be dissolved in distilled, deionized water to yield 1 liter each of 10X TBE buffer solution.
How to prepare a 10X TBE Buffer?
10X TBE buffer preparation is easy. Simply mix 10X TBE powder (SKU: A00269-500ml/A0026-4L) with an appropriate volume of distilled/deionized water. The 10X TBE buffer is ready to use now.
2-D Combination Packages
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Cleaver Scientific
Available in Mini, Mini-Wide, Maxi, WAVE and Maxi-Plus size formats, the Cleaver 2-D combination packages allow you to combine the CSL-IEF-KIT 1-D Combination Package, including flat-bed IEF unit, chiller and power supply, with a second-dimension vertical of your choice. 2-D Strip Throughput: 2x7cm IPG Strips.
Package Includes:
- Vertical of choice – see below.
- CSL-IEF
- CSL-CHILLER
- EV2320
- CSL-4-2
- IPG Converter kits, including 2 sets of special 2-D combs for 2x7cm IPG strips and plates with 10mm bonded spacers
2-Propanol EMPLURA® (Merck)
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Merck
Description
CAS Number: 67-63-0
Chemical Formula: CH₃CH(OH)CH₃
Molecular Weight: 60.1 g/mol
Synonyms: Isopropyl alcohol, Isopropanol, iso-Propanol, IPA
Quality Level: MQ200
2-Propanol gradient grade for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 67-63-0
Chemical Formula: CH₃CH(OH)CH₃
Molar Mass: 60.1 g/mol
Synonyms: Isopropyl alcohol, Isopropanol, iso-Propanol, IPA
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter
2-Propanol, for analysis EMSURE® ACS,ISO,Reag. Ph Eur (Merck)
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Merck
CAS no.: 67-63-0
Grade: ACS,ISO,Reag. Ph Eur
Chemical Formula: CH₃CH(OH)CH₃
Synonym: Isopropyl alcohol, Isopropanol, iso-Propanol, IPA
Quality level: MQ300
2,2-Diphenyl-1-picrylhydrazyl – Sigma – Aldrich
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Sigma-Aldrich
Description
CAS Number: 1898-66-4
Molecular weight : 394.32 g/mol
Empirical formula : C18H12N5O6
Synonyms : 1,1-Diphenyl-2-picrylhydrazyl radical, 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl, DPPH
2,2-diphenyl-1-picrylhydrazyl is a type of free radical that can accept hydrogen from antioxidants, making it a useful tool in the DPPH assay for evaluating the antioxidant activity of various natural samples, including wine, fruits, and herbal tea.
2,4,6-Tris(2-pyridyl)-s-triazine, For Spectrophotometric Det. of Fe (Sigma-Aldrich)
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Sigma-Aldrich
Description:
CAS number: 3682-35-7
Chemical Formula: C18H12N6
Molar Mass: 312.33 g/mol
Synonyms: 2,4,6-Tri(2-pyridyl)-s-triazine, TPTZ
General description
2,4,6-Tris(2-pyridyl)-s-triazine is a Case and Koft compound generally used in the determination of iron in a colourimetric analysis. This reagent is for the spectrophotometric determination of Fe(II) and of total Fe.
24 Leb Base 500g
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Thermo ScientificTM OxoidTM
Selectively enrich Listeria monocytogenes and other Listeria spp. from food and environmental samples with Thermo Scientific™ Oxoid™ 24 Listeria Enrichment Broth (24 LEB) (Dehydrated). The medium provides optimal growing conditions for all Listeria species for PCR applications. It is specifically designed for use with PCR systems, such as Thermo Scientific™ SureTect™ molecular assays for Listeria.
24 LISTERIA ENRICHMENT BROTH (24 LEB), Oxoid Composition
| Typical Formula * |
gm/litre
|
| Peptone |
23.4 |
| Yeast extract |
5.0 |
| Lithium chloride |
10.0 |
| Ferric ammonium citrate |
0.1 |
| Sodium chloride |
5.0 |
| pH 7.4 ± 0.2 @ 25°C |
24 LISTERIA ENRICHMENT BROTH (24 LEB), Oxoid Preparation:
Completely dissolve 21.75g of 24 LEB Base per 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add the contents of 1 vial of 24 LEB Selective Supplement per 500ml medium, reconstituted as directed. Cool to room temperature before use, and, once made, store the medium at 2-8°C in the dark.
3-Chloroperbenzoic acid, ≤77% (Sigma-Aldrich)
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Sigma-Aldrich
Description:
CAS number: 937-14-4
Chemical Formula: ClC6H4CO3H
Molar Mass: 172.57 g/mol
Synonyms: 3-Chloroperoxybenzoic acid, meta-Chloroperbenzoic acid, MCPBA
General description
3-Chloroperbenzoic acid is a strong oxidizing agent mainly used for the epoxidation of alkenes and also in the Baeyer-Villiger oxidation of ketones to esters.
Application
3-Chloroperbenzoic acid has been used in the oxidation of N–p-tolylsulfinyl-(E)-1-phenylethylideneimine to N–p-tolylsulfonyl (E)-1-phenylethylideneimine using dichloromethane as a solvent. It has also been used in the preparation of mono- and di-epoxy-functionalized poly(3-hydroxybutyrate)-based reactive polymers. MCPBA is also an effective oxidizing agent for the conversion of α-amide substituted polyesters to their corresponding polysulfones with tunable thermal properties.
Effective oxidant for epoxidizing di-, tri-, and tetra-substituted olefins.
4-Nitrophenyl butyrate, ≥98% (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 2635-84-9
Chemical Formula: C10H11NO4
Molar Mass: 209.20 g/mol
Synonyms: p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester
General description
4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.
Application
4-nitrophenyl butyrate has been used:
• as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
• as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
• as a substrate to determine the effect of buffers and solvents on human carboxylesterase
4D-Nucleofector™ System
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Lonza
Description
The 4D-Nucleofector™ System was developed to offer advanced performance, flexibility and convenience. The system is controlled by intuitive software on a touch screen user interface. Its modular architecture allows seamless expansion of the system for new applications. It comprises a core unit and different functional units:
The Core Unit – Controlling the 4D-Nucleofector™ System
The X unit – Supporting Nucleofection™ of various cell numbers in different formats
The Y unit – Enabling adherent Nucleofection™ in 24-well culture plates
Please contact us for pricing and availability.
50 bp DNA Ladder, NEB
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New England Biolabs
This product is available in our Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
- Size range: 50 bp – 1350 bp
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
- Easy-to-identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
5M NaCl, 1L
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Chemiz
Description
Synonyms: Salt, Halite
Formula: NaCl
5M NaCl is a colorless liquid that is commonly used in many molecular biology and forensic applications. For example, it is used to precipitate nucleic acids during the extractions.
7-deaza dGTP
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New England Biolabs
- 7-deaza-dGTP (7-deaza-2′-deoxyguanosine 5′-triphosphate) is supplied as a 5 mM solution in Milli-Q® water as a lithium salt
- The nucleotide concentration is determined by measurements of absorbance at 257 nm
