Microbiology

The Oxoid Peptone Bacteriological is for use whenever a high quality bacteriological peptone is required.

Oxoid Peptone Bacteriological Neutralized is the neutralized form of Peptone Bacteriological.

Oxoid Peptone Mycological is a mycological peptone developed for isolation of pathogenic and nonpathogenic fungi.

Thermo Scientific™ Oxoid Peptone P is a peptic digest which conforms to the USP specification of peptic digests of animal tissue.

• The high sulphur content makes it ideal for use in culture media

Oxoid Peptone Special is a specifically prepared mixture of peptones designed to encourage growth of the most fastidious microorganisms.

Peptone water (CM0009B), Oxoid, is a basal medium that used as a growth medium or as the basis of carbohydrate fermentation media. A pure culture in Peptone Water is a convenient inoculum for a series of fermentation tubes or other diagnostic media.

Directions

Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Oxoid Perfringens OPSP Selective Supplement A is for selective isolation of Clostridium perfringens from food.

• Add to Perfringens Agar Base, OPSP, Part No. CM0543B
• For 500mL of medium

Perfringens OPSP Selective Supplement A, Oxoid Composition

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Perfringens SFP Supplement is for the selective isolation of Clostridium perfringens.

• Add to Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens SFP Supplement, Oxoid Composition

Shahidi-Ferguson Perfringens Agar (SFP Agar) Preparation:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Perfringens TSC Supplement (D-cycloserine 200mg/vial) is used for the recovery of Clostridium perfringens.

• Add to DCLS Agar, Part No. CM0393B, or Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens TSC Supplement, Oxoid Composition

Agar Preparation:
To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.

To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Selectively isolate and presumptively identify Clostridium perfringens in food samples with Thermo Scientific™ Oxoid™ Perfringens TSC Agar Base (Dehydrated). The medium can either be used on its own or with selective agents to make Tryptose Sulphite (TS) Agar, Tryptose Sulphite Cycloserine (TSC) agar or Shahadi Ferguson Perfringens (SPF) agar for the presumptive identification and enumeration of Clostridium perfringens.

Perfringens TSC/SFP Agar Base, Oxoid Composition

Agar Preparation:
To Prepare the Agar Base:
Suspend 23g in 500ml of distilled water and heat gently until the agar is completely dissolved. Sterilise by autoclaving at 121°C for 10 minutes. Allow the medium to cool to 50°C.

To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.

To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.

To Prepare Shahidi-Ferguson Perfringens Agar (SFP Agar):
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.

To Prepare Agar for an Overlay:
For TSC or SFP Agar used as an overlay, the egg yolk emulsion (SR0047) is omitted. Its inclusion does not improve the lecithinase reaction and diminishes the visibility of the colonies.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Black colonies appearing on these two media may be organisms other than Clostridium perfringens.

Oxoid Perfringens OPSP Selective Supplement B is for selective isolation of Clostridium perfringens from food.

• Add to Perfringens Agar Base, OPSP, Part No. CM0543B
• For 500mL of medium

Perfringens OPSP Selective Supplement B, Oxoid Composition

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Determine the plate counts of microorganisms in food, dairy samples, waste water and other material of sanitary importance with Thermo Scientific™ Oxoid™ Plate Count Agar (Dehydrated). Plate Count Agar was developed by Buchbinder et al which is recommended by APHA, AOAC and PHLS. The quality control of Plate Count Agar (APHA) includes testing in accordance with ISO 11133:2014.

Plate Count Agar (Dehydrated), Oxoid Composition

Plate Count Agar (Dehydrated), Oxoid Preparation:
Add 17.5g to 1 litre of distilled water. Dissolve by bringing to the boil with frequent stirring, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Isolate and enumerate microorganisms in milk and dairy products with Thermo Scientific™ Oxoid™ Milk Plate Count Agar (Dehydrated). Milk Plate Count Agar is equivalent to the medium recommended by British Standard Institution and International Organization for Standardization.

Milk Plate Count Agar (Dehydrated), Oxoid Composition

Milk Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 19.5g in 1 litre of distilled water. Bring to the boil with frequent agitation, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Bacillus Cereus Selective Supplement (Polymyxin B Supplement) is supplied 50,000 IU/Vial for inhibition of Gram-negative bacteria.

• May be added to Bacillus cereus Selective Agar Base, Part No. CM0617B and MYP Agar Base, Part No. CM0929B
• Each vial supplements 500mL of medium

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Composition

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Preparation:
Reconstitute as directed in the product instructions for use. Aseptically, add one vial to 500ml of liquid or molten base medium, which is below 50°C. Mix well, and dispense into appropriate, sterile containers.

Storage conditions and Shelf life
Polymixin B Supplement at 2-8°C and use before the expiry date on the label.

Thermo Scientific™ Oxoid Potassium Lactate is a solution for addition to culture media, Lysine Medium CM0191 for example.

Oxoid Potassium Tellurite (3.5%) is a solution for addition to culture media.

Ordering Information:

Supplied 2mL/vial

Isolate and enumerate yeasts and molds from dairy and food products and prepare Aspergillus niger for the Harmonised Microbial Limit Tests from international pharmacopoeia with Thermo Scientific™ Oxoid™ Potato Dextrose Agar (Dehydrated). This medium is suitable for the detection and enumeration of heat resistant molds in thermally processed fruits and fruit products.

Pigment production is a critical part of the identification of the fungus. The agar used in the medium is carefully screened to ensure correct pigment production by fungi such as Fusaria spp.

Composition of Potato Dextrose Agar

Preparation of Potato Dextrose Agar, Oxoid
Suspend 39g in 1 litre of purified water and heat up to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Ensure the solution is well mixed before pouring.

To suppress the growth of bacteria, you can acidify the medium to pH 3.5 by adding 1 ml of lactic acid 10% SR0021 to each sterilized medium at 50°C. Note that the medium must not be heated after the addition of acid as it will destroy the gelling properties and results in hydrolysis of agar.

Storage Conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Thermo Scientific™ Oxoid Potato Dextrose Broth is a general broth for yeasts and molds.

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated) while adhering to BS ISO 21527-2: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated), Oxoid Composition

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base, Oxoid Preparation:
Suspend 15.8g per 500ml (31.6g/l) of distilled water and heat to dissolve completely. Before autoclaving add 220g of Glycerol per 1 litre of medium. Heat the complete solution gently to aid its dissolution of the glycerol. This has been found to be a key procedure to follow to allow proper dissolution of the glycerol. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Where bacterial overgrowth occurs from certain foods (raw meats) chlortetracycline hydrochloride at 50mg/L (dissolved in water and filter sterilised) is recommended.

Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date.

Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Pre-supplemented DG18 (ISO) Agar (CM1151) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.