Microbiology

Oxoid RPF Supplement (Rabbit Plasma Fibrinogen) is used for the isolation and enumeration of coagulase positive staphylococci from food.

• Use only with Baird-Parker Agar Base, RPF, Part No. CM0961B
• Each vial supplements 100mL of medium

RPF Supplement (Rabbit Plasma Fibrinogen), Oxoid Composition

RPF Supplement Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90 ml of sterile Baird-Parker Agar Base (RPF) CM0961 cooled to 48°C. Mix well and use immediately.

Precautions
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

Oxoid Cronobacter Sakazakii Isolation Agar (ISO) is a chromogenic medium for enrichment and isolation of Cronobacter spp. from milk and milk products in compliance with ISO/TS 22964: 2006.

E.Sak Isolation Agar Base, Oxoid Composition

E.Sak Isolation Agar, Oxoid Preparation:
Suspend 27.75g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium is best used freshly prepared, but may be suitable for longer storage following in-house validation.

The Thermo Scientific™ Oxoid Tryptone ia a pancreatic digest of casein for use where high performance and uniformity of composition are of paramount importance.

Cultivate anaerobic microorganisms, particularly Bacteroides spp. and other fastidious anaerobes with Thermo Scientific™ Oxoid™ Anaerobe Basal Agar (Dehydrated). Anaerobe Basal Broth is formulated from a range of nutrients which have been selected to optimize the recovery and growth of the majority of anaerobic organisms of clinical importance.

Anaerobe Basal Broth, Oxoid Composition

Anaerobe Basal Broth, Oxoid Preparation:
Suspend 35.4g of Anaerobe Basal Broth in 1 litre of distilled water. Bring to the boil to dissolve. Distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Anaerobe Basal Broth should be stored tightly capped in the original container at 10-30°C. When stored as directed, the medium will remain stable until the expiry date printed on the label.

Thermo Scientific™ Oxoid Tryptone Soya Broth (Tryptic Soy Broth) (Soybean Casein Digest Medium USP) is used for general laboratory use and sterility testing.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Detect and enumerate Escherichia coli in food and animal feed samples with Thermo Scientific™ Oxoid™ Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated). The medium meets the formulation and performance criterion laid down in ISO 16649 and ISO 11133:2014.

TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity, an enzyme shown to be highly specific for Escherichia coli.

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Composition

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Preparation:
Suspend 36.6g of TBX Medium in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour the medium into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Detect, enumerate and differentiate Escherichia coli and other coliforms by single membrane filtration with Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated). The presence of lauryl sulphate and lactose with phenol red allow the selective isolation and differentiation of Escherichia coli and coliforms respectively.

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Composition

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium may be stored for up to 1 week at 2-8°C.

• Brilliance Candida Agar Base is a chromogenic medium for the isolation and differentiation of clinically important Candida spp., including C. albicans.
• Also available Chromogenic Candida Agar Selective Supplement, Part No. SR0231E.

Brilliance Candida Agar Base, Oxoid Composition

Brilliance Candida Agar, Oxoid Preparation:
Suspend 15.6g in 500ml of distilled water. Add 1 vial of Brilliance Candida Selective Supplement reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool the medium to 45°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Brilliance Candida Agar Base must be stored tightly capped in the original container at 10-30°C. When stored as directed the unopened products will remain stable until the expiry date printed on the containers.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Brilliance Salmonella Agar Base is a chromogenic medium (formerly OSCM II) for isolation and presumptive identification of Salmonella spp. from food samples.

Brilliance Salmonella Agar Base, Oxoid Composition

 Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Brilliance Salmonella Agar should be stored in the tightly capped original container at 10-30ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Precautions
Brilliance Salmonella Agar is for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration.
Sterilise specimens, equipment and media properly after use.

Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony.

Brilliance Listeria Agar (ISO), Oxoid Composition

Brilliance Listeria Agar (ISO), Oxoid Preparation:

Suspend 34.5g in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool to 48°C. Aseptically add the contents of 1 vial of Brilliance™ Listeria Selective Supplement (ISO) (SR0257E) reconstituted as directed, and 1 vial of Brilliance™ Listeria Differential Supplement (ISO) (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium (CM1212B) at 10-30°C away from light and ensure bottles are correctly sealed after use.

Store prepared medium for up to 2 weeks at 2-8°C, away from light. Ensure all media is used before the expiry date on the label

Brilliance Bacillus Cereus Agar Base, Oxoid is a chromogenic medium for the isolation and differentiation of Bacillus cereus from food samples.

• Also available Chromogenic Bacillus Cereus Selective Supplement, Part No. SR0230E.

Brilliance Bacillus Cereus Agar Base, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

Precautions
X-glucopyranoside negative B. cereus strains may present white colonies on this medium due to poor or no utilisation of the chromogen.

Grow and maintain aerobic and anaerobic organisms with Thermo Scientific™ Oxoid™ Cooked Meat Medium (Dehydrated). The medium contains heart tissue, beef extracts, peptone and glucose for the cultivation of anaerobic and aerobic organisms. It has the ability to initiate bacterial growth from very small inocula and to maintain the viability of cultures over long periods of time1. Mixed cultures of bacteria survive in Cooked Meat Medium without displacing the slower growing organisms.

Cooked Meat Medium, Oxoid Composition

Cooked Meat Medium, Oxoid Preparation:
Suspend 10g in 100ml of distilled water (or 1g amounts in 10ml volumes of water in tubes). Allow to stand for 15 minutes until the meat particles are thoroughly wetted. Sterilise by autoclaving at 121°C for 15 minutes. Do not cool the bottles rapidly because ebullition will expel the meat particles from the containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature, in the dark with tightened caps for up to 6 months.

Precautions
The excellent recovery properties of Cooked Meat Medium mean that mixed cultures commonly result from sample inoculation.
Blackening of the medium will not take place if the pH is acid.
Carbohydrate fermentation may inhibit proteolysis.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water, Oxoid Composition

Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.

Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.

• Oxoid Chromogenic Salmonella Agar Base is a selective chromogenic medium for the presumptive identification of Salmonella spp. from clinical and food samples.
• Also available Salmonella Selective Supplement, Part No. SR0194E and Brilliance™ Salmonella Agar Base, Part No. CM1092B.

Chromogenic Salmonella Agar Base, Oxoid Composition

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 6 weeks at 2-8°C.

• Oxoid Clostridium Difficile Selective Supplement is added to Clostridium Difficile Agar Base, Part No. CM0601B.
• Each vial supplements 500mL of medium.

Clostridium Difficile Selective Supplement, Oxoid Composition

Clostridium Difficile Agar, Oxoid Preparation:
Suspend 34.5g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and add aseptically the contents of 1 vial of Oxoid Clostridium Difficile Supplement (SR0096) reconstituted as directed, together with 7% (v/v) Defibrinated Horse Blood (SR0050). Sheep Blood (SR0051) may be used in place of Horse Blood but some strains of the organism will show a slightly reduced growth recovery. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C no longer than 5-7 days.

Oxoid Burkholderia Cepacia Selective Supplement is used for the isolation of Burkholderia cepacia.

• Add to Burkholderia Cepacia Agar Base, Part No. CM0995B
• Each vial supplements 500mL of medium

Burkholderia Cepacia Selective Supplement, Oxoid Composition

Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between Escherichia coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies

Brilliance E. coli/coliform Selective Agar, Oxoid Composition

Brilliance E. coli/coliform Selective Agar, Oxoid Preparation:
Suspend 28.1g of Brilliance E. coli/coliform Selective Agar in 1 litre of distilled water. Bring the medium gently to the boil, to dissolve completely. Either pour the medium into sterile Petri dishes or keep molten at 45ºC for pour plate technique.

Storage conditions and Shelf life
Dehydrated medium: store tightly capped in the original container at 10-30°C
Prepared medium will be stable for up to 2 weeks when stored at 2-8ºC

Description

This DG 18 (Dichloran glycerol chloramphenicol) agar (base) is tested according to ISO 21527 and FDA-BAM GranuCult®. DG 18 Agar is suitable to enumerate and isolate xenophilic moulds from dried and semi-dried foods such as dried fruits, spices, confectionery, cereals, nuts, and dried meat and fish products.

Oxoid Perfringens TSC Supplement (D-cycloserine 200mg/vial) is used for the recovery of Clostridium perfringens.

• Add to DCLS Agar, Part No. CM0393B, or Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens TSC Supplement, Oxoid Composition

Agar Preparation:
To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.

To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.