ProteoExtract® Transmembrane Protein Extraction Kit
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Merck
Description
The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
ReBlot Plus Antibody Stripping Solution, 10x
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Merck
Description
ReBlot™ Plus reagents efficiently strip probed blots of
bound antibodies. ReBlot™ Plus reagents are available
in two formulations, “Mild” and “Strong”.
• Re-Blot™ Plus Mild Stripping Solution – Provides
good results on both nitrocellulose and PVDF
membranes.
• Re-Blot™ Plus Strong Stripping Solution –
Performs when membranes with high signal are
to be stripped, or use when Re-Blot™ Plus Mild
treatment is not sufficient.
Key Benefits
• β-Mercaptoethanol-free to avoid pungent smells
• Room temperature stripping in only 15 minutes
• Fast reuse of blots for multiple antibody probings
• Non-acidic, for less risk of protein degradation
(such as in Edman degradation)
RIPA Lysis Buffer, 10X, 100 mL
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Merck
Description
100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.
Key applications:
– immunoprecipitation
– western blotting
SignalBoost™ Immunoreaction Enhancer Kit
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Merck
Description
SignalBoost™ Immunoreaction Enhancer Kit is designed to enhance the desired signal in assays such as immunoblotting, dot blotting, and ELISA. This kit significantly improves sensitivity and discrimination when assessing protein targets of low immunoreactivity or low expression levels. Enhancement of signal is especially marked for low affinity antibodies. It replaces conventional antibody diluents with Solution 1, which is used to dilute primary antibodies and Solution 2, which is used to dilute secondary antibodies. No additional steps, materials, or increase of assay time is required.
• Increases signal: minimizes background resulting in high signal to noise ratios
• Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems
• Convenient: ready-to-use format, no dilution necessary
YeastBuster™ Protein Extraction Reagent (for yeast cell lysis)
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Merck
Description
YeastBuster™ Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
