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Color Prestained Protein Standard, Broad Range (10-250 kDa)

Brand:

New England Biolabs

The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed.

  • Allows approximate molecular weight determination when performing SDS-PAGE analysis
  • Applications include verification of Western transfer efficiency on membranes and fluorescent imaging of SDS-PAGE
  • Direct loading, additional loading buffer and heat incubation not required
  • Recombinant proteins with no detectable protease contaminating activities
  • Optimal stability for up to 24 months

OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer

Brand:

New England Biolabs

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

  • Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
  • Obtain high yields across a wide range of AT / GC content
  • Offered with inert tracking dyes for easy and direct loading of PCR products onto gels

Monarch® Total RNA Miniprep Kit

Brand:

New England Biolabs

Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types – all with Monarch Total RNA Miniprep kit!

  • For use with blood, cells and tissues
  • Also works with tough to lyse samples (bacteria, yeast, plant)
  • Effectively purifies total RNA of all sizes, including small RNAs >20 nt
  • Efficient genomic DNA removal (column and DNase I-based)
  • Contains Proteinase K for processing of tissues and blood samples
  • Includes RNA Protection Reagent for sample preservation
  • Excellent value
  • Kit components available separately

** You may also interest in RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit.

** Tips for using Monarch RNA Cleanup Kits 

Monarch PCR and DNA Cleanup Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify high-quality DNA from PCR and other enzymatic reactions.

  • Elute in as little as 6 μl.
  • Prevent buffer retention and salt carry-over with optimized column design.
  • Save time with fast, user-friendly protocols.
  • No need to monitor pH.
  • Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments.
  • Buffers and columns available separately.
  • Significantly less plastic used when compared with other kits.
  • Responsibly-sourced and recyclable packaging.

** Tips for using Monarch PCR and DNA Cleanup Kit

Monarch DNA Gel Extraction Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify DNA from agarose gels with high yields.

  • Elute in as little as 6 μl.
  • Prevent buffer retention and salt carry-over with optimized column design.
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol.
  • Buffers and columns available separately.
  • Significantly less plastic used when compared with other kits.
  • Responsibly-sourced and recyclable packaging.

** Tips for using Monarch DNA Gel Extraction kit

Monarch Plasmid Miniprep Kit

Brand:

New England Biolabs

The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of up to 20 μg of high quality plasmid DNA.

  • Elute in as little as 30 μl
  • Prevent buffer retention and salt carry-over with optimized column design
  • Reduce hands on time with faster protocols and less spin time
  • Monitor completion of certain steps using colored buffer system
  • No need to add RNase before starting
  • Easily label columns using tab and frosted surfaces
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging
  • No hazardous materials fees

** Tips for using Monarch Plasmid Miniprep Kit

Trypsin-digested BSA MS Standard (CAM-modified) – 500 pmol

Brand:

New England Biolabs

This standard is a complex mixture of peptides produced by the tryptic digest of reduced and alkylated Bovine Serum Albumin (BSA).

  • Used to standardize MALDI-TOF or ESI mass spectrometers (TOF, Q-TOF, Ion Trap, or Orbitrap)
  • Standardization range of 500-2400 Da
  • Free of salts, glycerol and detergents
  • Supplied lyophilized
  • Optimal stability for up to 24 months

Proteinase K, Molecular Biology Grade – 2 ml

Brand:

New England Biolabs

Proteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates.

  • Highly characterized for more consistent performance
  • Optimal activity and stability for up to 24 months
  • Active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0.
  • No detectable endonuclease, exonuclease, DNase or RNase contaminating activities

Trypsin-ultra Mass Spectrometry Grade – 100 ug

Brand:

New England Biolabs

Trypsin-ultra, Mass Spectrometry Grade is a serine endopeptidase, which selectively cleaves peptide bonds C-terminal to lysine and arginine residues.  Trypsin-ultra cleaves at Lys-Pro and Arg-Pro bonds at a much slower rate than when Lys and Arg are N-terminal to other residues.

  • Analyze complex proteomes with minimal autolysis
  • Compatible for simultaneous co-digestion with PNGase F (NEB# P0709)
  • Compatible for simultaneous co-digestion with Endoproteinase LysC (NEB# P8109)
  • Suitable for both in-gel and solution digests
  • Supplied lyophilized
  • Optimal activity and stability for up to 12 months

Blue Prestained Protein Standard, Broad Range (11-250kDa)

Brand:

New England Biolabs

This product is a direct replacement of NEB# P7706 (Blue Prestained Protein Standard, Broad Range 11-190 kDa).

The Blue Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed.

– Allows approximate molecular weight determination when performing SDS-PAGE analysis.
– Applications include verification of Western transfer efficiency on membranes and fluorescent imaging of SDS-PAGE.
– Direct loading, additional loading buffer and heat incubation not required
– Recombinant proteins with no detectable protease contaminating activities
– Optimal stability for up to 24 months

Unstained Protein Standard, Broad Range (10-200 kDa)

Brand:

New England Biolabs

This product is a direct replacement of NEB# P7704 (Unstained Protein Standard, Broad Range 10-200 kDa).

The Unstained Protein Standard, Broad Range is a mixture of highly pure, recombinant proteins that resolves into 13 sharp bands from 10-200 kDa when electrophoresed and stained with Coomassie Brilliant Blue R-250 or other commercially available, ready-to-use protein stains.

– Allows accurate molecular weight determination when performing SDS-PAGE analysis.
– Direct loading, additional loading buffer and heat incubation not required
– Recombinant proteins with no detectable protease contaminating activities
– Optimal stability for up to 24 months

Quick-Load 1 kb Extend DNA Ladder – 125 gel lanes

Brand:

New England Biolabs

  • Size range: 0.5 kb to 48.5 kb
  • Convenient, ready-to-load format
  • Formulated with bromophenol blue dye
  • Easy to identify reference bands
  • Suitable for 125 gel lanes

50 bp DNA Ladder, NEB

Brand:

New England Biolabs

This product is available in our Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

  • Size range: 50 bp – 1350 bp
  • Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Easy-to-identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

PCR Marker

Brand:

New England Biolabs

  • Size range: 50 bp to 766 bp
  • Value pricing
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes

1 kb DNA Ladder, NEB

Brand:

New England Biolabs

This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.

  • Size range: 500 bp to 10 kb
  • Value pricing
  • Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

1 kb Plus DNA Ladder, NEB

Brand:

New England Biolabs

This 1kb plus DNA ladder, NEB was previously named the 2-Log DNA Ladder. There have been no changes to product formulation or specifications. This product is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

A single convenient ladder for 100 bp up to 10 kb (100 bp and 1 kb ladder in one!)

  • Value pricing
  • Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

 

microRNA Marker

Brand:

New England Biolabs

  • Contains 2 synthetic oligonucleotides: 17, 21 and 25 residues
  • Convenient, ready-to-load format
  • For use with denaturing polyacrylamide gels and Northern blots

Quick-Load Purple Low Molecular Weight DNA Ladder – 125 gel lanes

Brand:

New England Biolabs

  • Convenient, ready-to-load format with NEB’s proprietary purple loading dye
  • Size range: 25bp to 766 bp
  • Sharp bands
  • No UV Shadow
  • Easy to identify reference bands
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Suitable for 125 gel lanes

Quick-Load Purple 50 bp DNA Ladder – 250 gel lanes

Brand:

New England Biolabs

  • Convenient, ready-to-load format with NEB’s proprietary purple loading dye
  • Size range: 50 bp to 1,350 bp
  • Sharp bands
  • No UV Shadow
  • Easy to identify reference bands
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Suitable for 250 gel lanes

Quick-Load Purple 1 kb DNA Ladder – 125 gel lanes, NEB

Brand:

New England Biolabs

Quick-Load Purple 1kb DNA Ladder, NEB is a molecular weight DNA marker with one tracking dye. It is a pre-mixed and a ready-to-load DNA ladder, which provides convenience to you.

Features of Quick-Load Purple 1kb DNA Ladder, NEB:

  • Convenient, ready-to-load format utilizing NEB’s proprietary purple loading dye
  • Size range: 500 bp to 10 kb
  • Sharp bands
  • No UV Shadow
  • Easy to identify reference bands
  • Now supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)

Quick-Load Purple 100 bp DNA Ladder – 125 gel lanes

Brand:

New England Biolabs

Quick-Load® Purple 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.

  • Convenient, ready-to-load format with NEB’s proprietary purple loading dye
  • Size range: 100 bp  to 1,517 bp
  • Sharp bands
  • No UV Shadow
  • Easy to identify reference bands
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)

Quick-Load Purple 1kb Plus DNA Ladder (0.1-10.0 kb) – 250 gel lanes

Brand:

New England Biolabs

This product was previously named the Quick-Load® Purple 2-Log DNA Ladder. There have been no changes to product formulation or specifications.

  • Convenient, ready-to-load format with NEB’s proprietary purple loading dye
  • Size range: 100 bp to 10 kb (100 bp and 1 kb ladder in one!)
  • Sharp bands
  • No UV Shadow
  • Easy to identify reference bands
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)

Supercoiled DNA Ladder – 100 gel lanes

Brand:

New England Biolabs

The Supercoiled DNA ladder contains 9 proprietary supercoiled plasmids, ranging in size from 2 to 10 kb, that are suitable for use as supercoiled molecular weight standards for agarose electrophoresis. The 5 kb plasmid has an increased intensity to serve as a reference band.

  • Recommended gel percentage range: 0.6-1%
  • Optimum separation on 0.8%
  • Recommended migration conditions: no more than 5V/cm (distance between electrodes), for no more than 60 minutes

Quick-Load 1kb Plus DNA Ladder – 250 gel lanes

Brand:

New England Biolabs

This product was previously named the Quick-Load® 2-Log DNA Ladder. There have been no changes to product formulation or specifications.This product is available in Quick-Load Purple format – enjoy sharper bands, no UV shadow, and a lower price. 

  • Size range:  100 bp to 10 kb
  • Convenient, ready-to-load format
  • Formulated with bromophenol blue dye
  • Easy to identify reference bands
  • Suitable for 250 gel lanes