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Q5 Hot Start High-Fidelity DNA Polymerase

Brand:

New England Biolabs

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

Q5 High-Fidelity 2X Master Mix

Brand:

New England Biolabs

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

Q5® High-Fidelity DNA Polymerase, NEB

Brand:

New England Biolabs

Q5® High-Fidelity DNA Polymerase, NEB sets a new standard for both fidelity and robust performance. With the highest-fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Features of Q5® High-Fidelity DNA Polymerase, NEB:

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer

Brand:

New England Biolabs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases for use with routine and difficult PCR experiments.

• Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step

• Obtain high yields with GC rich templates (>65%)

• Offered with inert tracking dyes for easy and direct loading of PCR products onto gels

OneTaq Quick-Load 2X Master Mix with Standard Buffer

Brand:

New England Biolabs

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

  • Obtain high yields across a wide range of AT- and GC- content
  • 2X higher fidelity than Taq
  • Master mix format contains inert tracking dye for easy and direct loading of PCR products onto gels

OneTaq Hot Start 2X Master Mix with GC Buffer

Brand:

New England Biolabs

OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

  • Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
  • Obtain high yields with GC rich templates (>65%)
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification

OneTaq Hot Start 2X Master Mix with Standard Buffer

Brand:

New England Biolabs

OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

  • Obtain high yields across a wide range of AT / GC content
  • 2X higher fidelity than Taq
  • Hot Start feature enables room temperature reaction setup
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification

OneTaq 2X Master Mix with Standard Buffer

Brand:

New England Biolabs

OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

  • Obtain high yields across a wide range of AT / GC content
  • 2X higher fidelity than Taq
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification

OneTaq Hot Start DNA Polymerase

Brand:

New England Biolabs

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.

  • Unique hot start formulation eliminates non-specific amplification and does not require a separate activation step
  • Robust yield with minimal optimization
  • Ideal for routine, AT- or GC-rich templates
  • Room temperature reaction setup

OneTaq DNA Polymerase

Brand:

New England Biolabs

The ONE polymerase for your endpoint PCR needs. OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

  • 2X higher fidelity than Taq
  • Ideal for routine, AT- or GC-rich templates
  • Hot start and master mix versions available

Cas9 Nuclease, S. pyogenes

Brand:

New England Biolabs

Bst 3.0 DNA Polymerase

Brand:

New England Biolabs

Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.

Shrimp Alkaline Phosphatase (rSAP)

Brand:

New England Biolabs

Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source.

  • Rapid and irreversible heat inactivation eliminates unwanted activity
  • Improved storage stability versus native enzyme
  • Faster reaction setup (no supplemental additives like zinc required)
  • Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
  • Less enzyme required (high specific activity), resulting in a lower cost per reaction
  • No need for multiple phosphatases (rSAP removes 5´- and 3´- phosphates from DNA, RNA and dNTPs )
  • Active on unincorporated dNTPs in PCR products – improves DNA sequencing and SNP analysis
  • Recombinant for purity, consistency and value

Instant Sticky-end Ligase Master Mix

Brand:

New England Biolabs

Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.

  • No incubation time necessary to achieve ligation
  • Efficiencies sufficient for successful sub-cloning of sticky-end substrates
  • No thawing of the master mix is required as liquid state is maintained when stored at -20°C
  • Simplified reaction setup with optimized ratio of enzyme and buffer components
  • Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

ElectroLigase – 50 rxns

Brand:

New England Biolabs

  • Directly compatible with electrocompetent cells
  • No thawing needed as it maintains liquid form at -20°C
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Blunt/TA Ligase Master Mix

Brand:

New England Biolabs

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates.

  • Simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components
  • No thawing is necessary as it remains liquid during storage at -20°C
  • Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Brand:

New England Biolabs

The industry standard

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • Mg-free buffer offers flexibility to adjust magnesium concentrations for use with microarray or DHPLC applications

DNase I (RNase-free)

Brand:

New England Biolabs

  • Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends
  • Degrades DNA template in transcription reactions
  • Removes contaminating genomic DNA from RNA samples
  • Isolated from a recombinant source

LongAmp Taq 2X Master Mix

Brand:

New England Biolabs

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb
  • 2X higher fidelity than Taq
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification of complex or simple templates

Bst DNA Polymerase Lg Frag

Brand:

New England Biolabs

The original polymerase for Loop-Mediated Isothermal Amplification (LAMP)

  • Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity
  • Suitable for applications requiring thermophilic strand displacement
  • Supplied with ThermoPol® Reaction Buffer for high product yields in demanding conditions

Taq DNA Polymerase with Standard Taq Buffer, NEB

Brand:

New England Biolabs

Taq DNA Polymerase, NEB is a thermostable DNA polymerase that possesses 5′ to 3′ polymerase activity, that makes it an excellent industry standard for PCR.

Key features of Taq DNA Polymerase, NEB:

  • Isolated from a recombinant source
  • Robust and reliable reaction
  • Incorporates dUTP, dITP and fluorescently -labeled nucleotides
  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • Hot start version available

Taq DNA Polymerase, NEB is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.

Quick-Load Taq 2X Master Mix – 500 rxns

Brand:

New England Biolabs

The industry standard

  • Exceptional value in terms of cost per unit
  • 2X master mix format for easy reaction setup – just add template DNA and primers
  • Offered with inert tracking dyes for easy and direct loading of PCR products onto gels

Taq 2X Master Mix – 500 rxns

Brand:

New England Biolabs

Taq DNA Polymerase is the industry standard

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • 2X master mix format for easy reaction setup – just add template DNA and primers

Taq DNA Polymerase with ThermoPol Buffer

Brand:

New England Biolabs

Taq DNA Polymerase is the industry standard for PCR

  • Ideal for routine PCR applications
  • ThermoPol Buffer delivers high product yield under demanding conditions
  • Exceptional value in terms of cost per unit