M-MuLV Reverse Transcriptase
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New England Biolabs
M-MuLV Reverse Transcriptase synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Tested for the absence of DNases and RNases
T4 DNA Ligase, NEB
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New England Biolabs
Characteristics of T4 DNA Ligase, NEB:
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
- This enzyme will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
- T4 DNA ligase will seal nicks for these DNA substrates.
- The industry standard for performance and quality
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
ProtoScript II First Strand cDNA Synthesis Kit
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New England Biolabs
The ProtoScript® II First Strand cDNA Synthesis Kit is optimized mix for higher specificity and yield of cDNA.
- Recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability (active up to 48°C)
- Generates cDNA up to 10 kb
- Supplied with Oligo-dT primers, Randomized Primer Mix, and nuclease-free water
OneTaq One-Step RT-PCR Kit – 30 rxns
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New England Biolabs
Easy and efficient One-Step RT-PCR
- Optimized reaction mix for cDNA synthesis and PCR amplification in one step
- Hot start formulation eliminates nonspecific amplification and does not require a separate activation step
- Offered with Quick-Load® dyes for sample tracking and direct loading of PCR products onto gels
Luna Universal Probe One-Step RT-qPCR Kit
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New England Biolabs
Make a simpler choice
- One product per application simplifies selection
- Convenient master mix formats and user-friendly protocols simplify reaction setup
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Experience best-in-class performance
Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase
Luna Universal One-Step RT-qPCR Kit
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New England Biolabs
Make a simpler choice
- One product per application simplifies selection
- Convenient master mix formats and user-friendly protocols simplify reaction setup
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Experience best-in-class performance
Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase
WarmStart LAMP Kit (DNA and RNA)
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New England Biolabs
- Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
- Compatible with multiple detection methods
- WarmStart® technology inhibits activity at room temperature
NEB PCR Cloning Kit (Without Competent Cells) – 20 rxns
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New England Biolabs
Easy cloning of all PCR products, including blunt and TA ends
- In vitro transcription with both SP6 and T7 promoters
- BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules)
- Fast cloning experiments with 5-minute ligation step
- Simplified screening with low/no colony background and no blue/white selection required
Q5 High-Fidelity PCR Kit
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New England Biolabs
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot Start and Master Mix formats available
Phusion High-Fidelity PCR Kit
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New England Biolabs
Ideal for high speed and high-performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific®Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust Reactions – maximal success with minimal optimization
- Kit includes everything needed for a PCR reaction, including controls
Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) – 10 rxns
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New England Biolabs
The Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.
- Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
- Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
- Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
Magnesium Chloride (MgCl2) Solution – 6.0 ml
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New England Biolabs
25 mM MgCl2 for use in conjunction with polymerase reaction buffers.
- MgCl2 is available for use in conjunction with NEB’s polymerase reaction buffers
- Supplied as 4 x 1.5 ml vials
Recombinant Albumin, Molecular Biology Grade
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New England Biolabs
Recombinant Albumin (rAlbumin), Molecular Biology Grade, is a non-bovine derived albumin that can serve as an alternative to Bovine Serum Albumin (BSA). Like BSA, it has been shown to prevent adhesion of enzymes to reaction tubes and pipette surfaces. It also stabilizes some proteins during incubation. Choose Recombinant Albumin, when there is a need to avoid BSA.
Gel Loading Dye, Purple (6X), no SDS – 4.0 ml, NEB
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New England Biolabs
Gel Loading Dye, Purple (6X), NEB is the premier purple loading dye for sharp, tight bands.
- No UV shadow
- Contains Ficoll® for brighter, tighter bands
- Contains EDTA to stop enzymatic reactions
- Compatible with agarose and non-denaturing polyacrylamide gels
- No UV shadow allows for publication grade images
- Brighter, sharper bands vs. glycerol-based loading dyes
- Stops enzyme reactions
Magnesium Sulfate (MgSO4) Solution – 6.0 ml
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New England Biolabs
100 mM MgSO4 for use in conjunction with polymerase reaction buffers.
Microbat 2009 Windows V2.04
Login to see pricesEasily read and interpret Microbact results with the Thermo Scientific™ Oxoid™ Microbact™ Software Program. Results are clearly visible as distinct color reactions, creating individualized microcodes for more precise reporting.
Vegetable Peptone No. 1 500g
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Vegetable Peptone No. 1 is a highly nutritious, meat-free general purpose peptone for growth of bacteria and fungi.
Dessicant Sachet
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Thermo ScientificTM OxoidTM
Maintain a low moisture environment when storing AST products for manual Antimicrobial Susceptibility Testing with Thermo Scientific™ Oxoid™ Replacement Desiccant (Dispenser).
Disk Dispenser 8 Place 100mm Skirt
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Thermo ScientificTM OxoidTM
Accurately place antimicrobial susceptibility disks onto your media of choice when carrying out manual Antimicrobial Susceptibility Testing methods using a self-tamping Thermo Scientific™ Oxoid™ Antimicrobial Susceptibility Disk Dispenser.
Disk Dispenser 8 Place 90mm Skirt
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Thermo ScientificTM OxoidTM
Accurately place antimicrobial susceptibility disks onto your media of choice when carrying out manual Antimicrobial Susceptibility Testing methods using a self-tamping Thermo Scientific™ Oxoid™ Antimicrobial Susceptibility Disk Dispenser.
Disk Dispenser 6 Place 100mm Skirt
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Thermo ScientificTM OxoidTM
Accurately place antimicrobial susceptibility disks onto your media of choice when carrying out manual Antimicrobial Susceptibility Testing methods using a self-tamping Thermo Scientific™ Oxoid™ Antimicrobial Susceptibility Disk Dispenser.
Disk Dispenser 6 Place 90mm Skirt
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Thermo ScientificTM OxoidTM
Accurately place antimicrobial susceptibility disks onto your media of choice when carrying out manual Antimicrobial Susceptibility Testing methods using a self-tamping Thermo Scientific™ Oxoid™ Antimicrobial Susceptibility Disk Dispenser.
Vancomycin Supplement (5mg)
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.
- Each vial supplements 500mL of medium
Vancomycin Supplement, Oxoid Composition
| Vial contents: (each vial sufficient for 500ml of medium) |
per vial
|
per litre
|
| Vancomycin |
5mg
|
10mg
|
Cronobacter Screening Broth (CSB), Oxoid Preparation:
Dissolve 14.02g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.
Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
One Broth-Salmonella Supplement 10x225ML
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Supplement is for the selective enrichment of Salmonella spp. from food samples in 24 hours in a single enrichment step.
- Add to ONE Broth-Salmonella Part No. CM1091B
- Each vial supplements 500mL of medium
ONE Broth Salmonella Selective Supplement, Oxoid Composition
| Vial Contents (each vial is sufficient for 500ml of medium) |
per vial |
per litre |
| Novobiocin |
2.7mg |
12mg |
ONE Broth Salmonella Selective Medium Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.
Storage conditions and Shelf life
The ONE Broth-Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.
Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.
