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Selectively isolate Salmonella species from food samples with Thermo Scientific™ Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn) for food testing in compliance with ISO 6579:2002.

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Composition

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Preparation:
Suspend 89.5g of MKTTn in 1 litre of distilled water, mix well and bring to the boil. Cool to below 45°C. Immediately before use add 20ml of iodine-iodide solution prepared by dissolving 25g of potassium iodide in 10ml of water, adding 20g of iodine and then diluting to 100ml with sterile water. Also add the contents of four vials of Oxoid Novobiocin Selective Supplement SR0181 reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at room temperature. The prepared medium in its final form containing iodine/iodide solution + Novobiocin Selective Supplement SR0181 should be used immediately.

Oxoid MLCB Agar is used for the selective isolation of Salmonella spp., not S.Typhi or S. Paratyphi A.

MLCB Agar, Oxoid Composition

MLCB Agar, Oxoid Preparation:
Suspend 49.0g in 1 litre of distilled water. Mix and bring gently to the boil with frequent agitation to dissolve the medium completely. Cool to 50°C and pour approximately 20ml into sterile Petri dishes.
DO NOT AUTOCLAVE OR OVERHEAT.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The identity of colonies presumed from their appearance to be Salmonella spp. must be confirmed by biochemical and serological testing. In common with other enteric media care must be taken to ensure the purity of colonies taken for further testing as organisms that are inhibited from developing into colonies remain viable and may accidentally be picked on sub-culture.

Detect, enumerate and differentiate Escherichia coli and other coliforms by single membrane filtration with Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated). The presence of lauryl sulphate and lactose with phenol red allow the selective isolation and differentiation of Escherichia coli and coliforms respectively.

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Composition

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium may be stored for up to 1 week at 2-8°C.

Oxoid Modified Bolton Broth Selective Supplement is for the selective pre-enrichment of Campylobacter spp. in food samples.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Bolton Broth, Part No. CM0983B
• Each vial supplements 500mL of medium

Modified Bolton Broth Selective Supplement, Oxoid Composition

Modified Bolton Broth Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Bolton Broth CM0983 cooled to approximately 50°C with 25 ml of laked horse blood SR0048. Mix well and distribute into sterile containers.

Oxoid Modified Brucella Selective Supplement is for the cultivation and isolation of Brucella spp.

• Alternative supplement containing natamycin as a replacement for cycloheximide
• Add to Blood Agar Base No. 2, Part No. CM0271B or Brucella Medium Base, Part No. CM0169B
• Each vial supplements 500mL of medium

Modified Brucella Selective Supplement, Oxoid Composition

Modified Brucella Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml Brucella Medium Base, Columbia Agar Base or Blood Agar Base No.2 together with 5-10% v/v inactivated horse serum SR0035 and 1-5% w/v sterile solution of glucose, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Oxoid Modified Karmali Selective Supplement is for a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42° C.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Campylobacter Agar Base (Karmali), Part No. CM0935B
• Each vial supplements 500mL of medium

Modified Karmali Selective Supplement, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Campylobacter Agar Base (Karmali) CM0935 cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Selectively enrich Cronobacter spp. from milk and milk samples using Thermo Scientific™ Oxoid™ Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated). Modified Lauryl Sulphate Tryptose Broth Base was originally described by Guillaume-Gentil et al. for the selective enrichment of Cronobacter spp. The high salt concentration of the medium restricts the growth of other members of Enterobacteriaceae and the combination of vancomycin and sodium lauryl sulphate inhibits the growth of Gram-positive organisms commonly isolated in production environments.

Modified Lauryl Sulphate Tryptose Broth Base, Oxoid Composition

Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label
Where possible the complete medium should be prepared on the day of testing and stored for no longer than 1 day at 0-5ºC

• Oxoid Listeria Selective Supplement (Oxford) is used for the detection of Listeria monocytogenes.
• Add to Listeria Selective Agar Base, Part No. CM0856B.

Modified Listeria Selective Supplement (Oxford), Oxoid Composition

Modified Listeria Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to500 ml of sterilised Listeria Selective Agar Base (Oxford Formulation) CM0856, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Oxoid Modified Preston Campylobacter Selective Supplement is for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian, and environmental samples.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Campylobacter Agar Base, Part No. CM0689B or Nutrient Broth No. 2, Part No. CM0067B
• Each vial supplements 500mL of medium

Modified Preston Campylobacter Selective Supplement, Oxoid Composition

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

• Oxoid Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium) is used for the detection of motile Salmonella spp. from food and environmental samples.
• Also available MSRV Selective Supplement, Part No. SR0161E and Novobiocin Supplement, Part No. SR0181E.

Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium), Oxoid Composition

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Precautions
The basal medium is very hygroscopic. When handling the powder a face mask and gloves must be worn, refer to the material safety data sheet.