Peptone Mycological 500g
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Thermo ScientificTM OxoidTM
Oxoid Peptone Mycological is a mycological peptone developed for isolation of pathogenic and nonpathogenic fungi.
Peptone P 500g
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Peptone P is a peptic digest which conforms to the USP specification of peptic digests of animal tissue.
- The high sulphur content makes it ideal for use in culture media
Peptone Special 500g
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Thermo ScientificTM OxoidTM
Oxoid Peptone Special is a specifically prepared mixture of peptones designed to encourage growth of the most fastidious microorganisms.
Peptone Water 500g, Oxoid
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Thermo ScientificTM OxoidTM
Peptone water (CM0009B), Oxoid, is a basal medium that used as a growth medium or as the basis of carbohydrate fermentation media. A pure culture in Peptone Water is a convenient inoculum for a series of fermentation tubes or other diagnostic media.
| Typical Formula* | gm/litre |
| Peptone | 10.0 |
| Sodium Chloride | 5.0 |
| pH 7.2 ± 0.2 @ 25°C |
Directions
Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.
Perfringens (OPSP) Selective Supplement A 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Perfringens OPSP Selective Supplement A is for selective isolation of Clostridium perfringens from food.
- Add to Perfringens Agar Base, OPSP, Part No. CM0543B
- For 500mL of medium
Perfringens OPSP Selective Supplement A, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Sodium sulphadiazine | 50.0mg | 100.0mg |
Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Perfringens (SFP) Select Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Perfringens SFP Supplement is for the selective isolation of Clostridium perfringens.
- Add to Perfringens Agar Base, TSC, Part No. CM0587B
- Each vial supplements 500mL of medium
Perfringens SFP Supplement, Oxoid Composition
| Vial contents | SR0093E | per litre |
| Kanamycin sulphate | 6.0mg | 12.0mg |
| Polymyxin B | 15,000IU | 30,000IU |
Shahidi-Ferguson Perfringens Agar (SFP Agar) Preparation:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Perfringens (TSC) Select Supplement 1 x 10 Vial
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Thermo ScientificTM OxoidTM
Oxoid Perfringens TSC Supplement (D-cycloserine 200mg/vial) is used for the recovery of Clostridium perfringens.
- Add to DCLS Agar, Part No. CM0393B, or Perfringens Agar Base, TSC, Part No. CM0587B
- Each vial supplements 500mL of medium
Perfringens TSC Supplement, Oxoid Composition
| Vial contents | SR0088E | per litre |
| D-cycloserine | 200.0mg | 400.0mg |
Agar Preparation:
To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.
To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Perfringens Agar Base TSC/SFP 500g
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Thermo ScientificTM OxoidTM
Selectively isolate and presumptively identify Clostridium perfringens in food samples with Thermo Scientific™ Oxoid™ Perfringens TSC Agar Base (Dehydrated). The medium can either be used on its own or with selective agents to make Tryptose Sulphite (TS) Agar, Tryptose Sulphite Cycloserine (TSC) agar or Shahadi Ferguson Perfringens (SPF) agar for the presumptive identification and enumeration of Clostridium perfringens.
Perfringens TSC/SFP Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptose | 15.0 |
| Soya peptone | 5.0 |
| Yeast extract | 5.0 |
| Sodium metabisulphite | 1.0 |
| Ferric ammonium citrate | 1.0 |
| Agar | 19.0 |
| pH 7.6 ± 0.2 @ 25°C |
Agar Preparation:
To Prepare the Agar Base:
Suspend 23g in 500ml of distilled water and heat gently until the agar is completely dissolved. Sterilise by autoclaving at 121°C for 10 minutes. Allow the medium to cool to 50°C.
To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.
To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.
To Prepare Shahidi-Ferguson Perfringens Agar (SFP Agar):
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.
To Prepare Agar for an Overlay:
For TSC or SFP Agar used as an overlay, the egg yolk emulsion (SR0047) is omitted. Its inclusion does not improve the lecithinase reaction and diminishes the visibility of the colonies.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Black colonies appearing on these two media may be organisms other than Clostridium perfringens.
Perfringens OPSP Selective Supplement B 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Perfringens OPSP Selective Supplement B is for selective isolation of Clostridium perfringens from food.
- Add to Perfringens Agar Base, OPSP, Part No. CM0543B
- For 500mL of medium
Perfringens OPSP Selective Supplement B, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Oleandomycin phosphate | 0.25mg | 0.5mg |
| Polymyxin B | 5,000IU | 10,000IU |
Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Petri Dishes
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Biologix
Characteristics
● Made of medical-grade polystyrene; High degree of transparency for optimal analysis by microscopy
● Three sizes available with uniform thickness and smooth surface
● Vented lid for better gas exchange; Easy stacking
Petroleum benzine for analysis boiling range 40-60°C EMSURE® ACS,ISO (Merck)
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Merck
Description
CAS number: 64742-49-0
Chemical Formula: –
Molar Mass: –
Synonyms: –
EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.
Product Specification and MSDS for Petroleum benzine EMSURE® (Merck).
Petroleum ether 40°C-60°C AR, 2.5L
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Chemiz
Description
Formula: C6H14
Analytical grade petroleum ether for laboratory uses as solvent and extraction solvent.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
pH-Indicator Strips, MQuant® (Merck)
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Merck
Description
The MQuant® pH tests are suited for all media in environmental analysis and in industrial in-process controls. Our special method of manufacture gives the pH indicator strips decisive advantages over the indicator paper: since the indicator does not bleed, the strip can be left in the measurement medium without contaminating it. Using these test strips is fairly easy, the pH value is measured semiquantitatively by visual comparison of the reaction zone on the test strip with the fields of the color scale, perfectly matching the reaction color. The clever Safetyedge boxes allow for safe storage and convenient withdrawal of the test strips. For tests for other parameters that require a specific pH range of the sample (e.g. Spectroquant®, see instructions of use), we recommend the use of MQuant® pH test strips as pre-test.
Product Specification and MSDS for MQuant® pH Indicator Strips (Merck).
Phenol Red Solution BTL/25ml
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Thermo Scientific™ Remel™
Thermo Scientific™ Remel Phenol Red Solution is a pH indicator for use with microbiological culture media.
Phorbol 12-myristate 13-acetate, ≥99% (TLC, Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 16561-29-8
Chemical Formula: C36H56O8
Molar Mass: 616.83g/mol
Synonyms: 12-O-Tetradecanoylphorbol 13-acetate, 4β,9α,12β,13α,20-Pentahydroxytiglia-1,6-dien-3-one 12-tetradecanoate 13-acetate, PMA, TPA
General description
Phorbol 12-myristate 13-acetate is a phorbol ester. It works as a tumour promoter and is involved in gene transcription, cell growth, differentiation, programmed cell death, immune pathway and receptor desensitization via protein kinase C (PKC) signalling pathways. Phorbol 12-myristate 13-acetate is also a model agent to study growth factors-, hormones-, and cytokines-mediated regulation of cellular growth and differentiation.
Application
Phorbol 12-myristate 13-acetate has been used for the stimulation of various cells including spleen cells, quiescent lymphocytes and fetal thymocytes. It has also been used for the activation of PKC (protein kinase C) in cells.
Solubility
DMSO: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions. DMSO solutions can be stored dark at -20 °C for at least six months.)
Ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions.)
Phosphate buffered saline (Sigma-Aldrich)
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Sigma
Description
CAS Number : 24867-26-3
Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.
Application :
- For the production of platinum nanoparticles (NP-Pt) hydrocolloids
- For the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM
- For preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)
- For the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)
- For suspension of cell pellets during centrifugation
- In immunoassay procedures.
One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.
Phosphate Buffered Saline Powder, pH 7.4, For Preparing 1 L solutions, Sigma
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Sigma-Aldrich
Application
• Phosphate buffered saline has been used in the washing solutions during immunofluorescence procedure.
• It has been used for the suspension of bacterial cells.
• It has been used during the fixation and immunostaining procedures.
Reconstitution
Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate-buffered saline (NaCl 0.138 M; KCl – 0.0027 M); pH 7.4, at 25 °C.
Phosphate Buffered Saline Tablets (PBS), Oxoid
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Thermo ScientificTM OxoidTM
Phosphate Buffered Saline Tablets, Dulbecco A (PBS, Oxoid) is used for the preparation of a balanced salt solution without calcium and magnesium. PBS is normally used to rinse the cells free from growth medium before application of the chelating agent, such as Versene (diamino-ethane-tetra-acetic acid).
Content of Phosphate Buffered Saline, Oxoid:
| Typical Formula * | gm/litre |
| Sodium Chloride | 8.0 |
| Potassium Chloride | 0.2 |
| Disodium Hydrogen Phosphate | 1.15 |
| Potassium Dihydrogen Phosphate | 0.2 |
| pH 7.3 ± 0.2 @ 25°C |
Preparation of PBS Buffer from PBS tablets, Oxoid
Dissolve ONE tablet in 100ml of distilled water. Sterilize the PBS solution with autoclaving for 115°C for 15 mins. The tablets should be dissolving completely and free from insoluble matter.
Phosphate buffered saline, 1X , pH 7.4, 1L
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Chemiz
Description
Component: NaCl, KCl, Na2HPO4, KH2PO4
General buffer widely used in biological applications such as dilution and cell washing as it is non-toxic and closely mimic the physiological conditions of human body.
Phusion High-Fidelity DNA Polymerase
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust reactions – maximal success with minimal optimization
- Offered with multiple buffers for customized reaction setup for different DNA templates
Phusion High-Fidelity PCR Kit
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New England Biolabs
Ideal for high speed and high-performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific®Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust Reactions – maximal success with minimal optimization
- Kit includes everything needed for a PCR reaction, including controls
Phusion High-Fidelity PCR Master Mix with GC Buffer
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Robust reactions – maximal success with minimal optimization
- Master mix format with GC buffer ideal for long, difficult, or GC-rich DNA templates
Phusion High-Fidelity PCR Master Mix with HF Buffer
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity compared to Taq
- Robust reactions – maximal success with minimal optimization
- Master mix format with HF buffer ideal for a wide range of AT/GC content
Phusion Hot Start Flex 2X Master Mix
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New England Biolabs
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity than Taq
- Hot start formulation reduces non-specific amplification
- Master mix format for ease of use and optimized performance
