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TLC Silica gel 60 F₂₅₄, (20×20 cm) – Merck

Brand:

Merck

Description

For thin-layer chromatography applications, we offer Aluminum TLC plates that are coated with silica gel containing a fluorescent indicator F254. These silica gel TLC plates are available in a set of 25 and measure 20x20cm. Our classical silica TLC plates are created by combining our established silica gel 60 with a unique polymeric binder. This combination produces a very adherent and robust surface that will not crack or blister and can even withstand writing with a pencil without risking damage to the layer.

Toluene for liquid chromatography LiChrosolv®

Brand:

Merck

Description

CAS number: 108-88-3
Chemical Formula: C₆H₅CH₃
Molar Mass: 92.14 g/mol
Synonyms: Toluol, Methylbenzene

With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter.

TRI Reagent® for processing tissues, cells cultured in monolayer or cell pellets (Sigma-Aldrich)

Brand:

Sigma

Description:

Synonyms : DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, TRI Reagent® cell pellet solution, cell pellet RNA extraction, cell pellet RNA isolation, cell pellet RNA purification, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction solution, total RNA isolation solution, total RNA purification solution, TRI Reagent® RNA Isolation Reagent.

TRI reagent® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagent® is a mixture of guanidine thiocyanate and phenol in a monophasic solution.

Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.

Trichloroacetic acid, AR, 500g

Brand:

Chemiz

Description

Synonyms: Trichloroethanoic acid

Formula: Cl3CCOOH

Tricholoroacetic acid is used in biology to facilitate the precipitation of macromolecules found in the sample. It is also used in the cosmetic industry as chemical peel and wart remover.

Note:

AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.

Triethylene glycol, ReagentPlus®, 99% (Sigma-Aldrich)

Brand:

Sigma-Aldrich

Description

CAS number: 112-27-6
Chemical Formula: HO(CH2CH2O)2CH2CH2OH
Molar Mass: 150.17g/mol
Synonyms: Triglycol

TWEEN® 20 (Sigma-Aldrich)

Brand:

Sigma-Aldrich

Description

CAS number: 9005-64-5
Suitability: Suitable for DNA extraction, RNA extraction, cell culture and protein purification
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 has been used as a component of:
• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry
• washing buffer in ELISA (enzyme-linked immunosorbent assay)
• reaction mixture in PCR (polymerase chain reaction)
• emulsifying agent for the preparation of stable oil-in-water emulsions.
• pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• lysing mammalian cells at a concentration of 0.005 to 0.5%

TWEEN® 20 BioXtra (Sigma-Aldrich)

Brand:

Sigma-Aldrich

Description

CAS number: 9005-64-5
Suitability: Suitable for blocking, cell culture, hybridization, protein purification, transformation
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is a non-ionic detergent that has been used in a variety of applications, including:

  • Post-hybridization wash buffer after in situ hybridization.
  • Used along with PBS (phosphate-buffered saline) as the washing buffer for ELISA and immunostaining.
  • Acted as an emulsifying agent for the preparation of stable oil-in-water emulsions
  • Used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
  • Acted as a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%.
  • Used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

TWEEN® 20 Detergent (Merck)

Brand:

Merck

Description

CAS number: 9005-64-5
Synonyms: Polysorbate 20, Polyoxyethylene (20) sorbitan monolaurate, PEG(20)sorbitan monolaurate
Form: Pale yellow liquid

Non-ionic detergent used for immunoprecipitation and for the solubilization of membrane-bound proteins.

Product Specification and MSDS for TWEEN® 20 Detergent (Merck).

TWEEN® 20 for Molecular Biology (Sigma-Aldrich)

Brand:

Sigma-Aldrich

Description

CAS number: 9005-64-5
Suitability: Suitable for molecular biology
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is a non-ionic detergent that widely used in biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• For lysing mammalian cells at a concentration of 0.005 to 0.5%
• Along with PBS in the dilution of antibodies in immunohistochemistry technique
• For washing cells in FISH (Fluorescence in situ hybridization) technique

Urea CP, 500g

Brand:

Chemiz

Description

Synonyms: Carbamide

Formula: NH2CONH2

Urea is a raw material used in the manufacture of many chemicals such as plastics and adhesives. It is highly soluble and has a pKa of almost 0.

Note:

AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.

Water for chromatography (LC-MS Grade) LiChrosolv®

Brand:

Merck

Description

CAS number: 7732-18-5
Chemical Formula: H₂O
Molar Mass: 18.02 g/mol
Synonyms: Deionized water

With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved.

Water Standard 0.1%, Certified Reference Material (Merck)

Brand:

Merck

CAS no.: 1589-47-5, 107-98-2
Traceability: Certified Reference Material for Karl Fischer Titration 1 g ≙ 1 mg H₂O Aquastar®
Synonym: –
Quality level: MQ300
Product specification and SDS for Water Standard 0.1%.

Water Standard 1%, CRM for Karl Fischer Titration (Merck)

Brand:

Merck

CAS no.: 1589-47-5, 107-98-2
Chemical Formula: –
Molecular Weight: –
Synonym: Water standard 1% Certified Reference Material for Karl Fischer Titration 1 g ≙ 10 mg H₂O Aquastar®
Quality level: MQ300
Product specification for Water standard 1% CRM for Karl Fischer Titration

Water Standard Oil, CRM for Karl Fischer Titration (Merck)

Brand:

Merck

CAS no.:
Chemical Formula: –
Molecular Weight: –
Synonym: Water Standard Oil Certified Reference Material for Karl Fischer Titration (oil matrix 15-30 ppm H2O) Aquastar®
Quality level: MQ300
Product specification and SDS for Water Standard Oil Certified Reference Material for Karl Fischer Titration (oil matrix 15-30 ppm H2O)

Zinc oxide for analysis EMSURE® ACS,Reag. Ph Eur

Brand:

Merck

Description

CAS number: 1314-13-2
Chemical Formula: ZnO
Molar Mass: 81.39 g/mol
Synonyms: –

EMSURE® grade solvents are suitable for a broad spectrum of classical lab applications and are frequently used in regulated and highly demanding lab applications. EMSURE® provides worldwide best and most extensive product specifications. We declare our EMSURE® range to be in compliance with the ACS, with the reagent part of the European Pharmacopoeia (Reag. Ph Eur) and also with the ISO standards.

Product Specification and MSDS for Zinc oxide EMSURE® (Merck).

Zinc Oxide, CP, 500g

Brand:

Chemiz

Description

Formula: ZnO

Zinc oxide has a variety of uses including rubber and ceramic glaze manufacture, UV absorber, paint corrosion inhibitor and as nutrient supplement.

Note:

AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.