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Exo-CIP™ Rapid PCR Cleanup Kit, NEB

Brand:

New England Biolabs

  • Rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification
  • Reaction complete in 4 minutes
  • Thermolabile formulation can be heat inactivated in 1 minute at 80°C
  • PCR product can be used directly in downstream applications
  • Compatible with commonly-used reaction buffers

FastDNA 50ml Spin Kit for Soil

Brand:

MP Biomedicals

Up to 5 g of soil are processed by the FastPrep®-24 or FastPrep®-96 instrument with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter columns and removes humic acids/polyphenols that can inhibit PCR. The resulting pure DNA is suitable for PCR analysis and other downstream applications. Research use only. Key applications include isolation of bacterial, fungi, plant and genomic DNA from soil and evnironmental samples.

FastDNA-96 Soil Microbe DNA Kit

Brand:

MP Biomedicals

The FastDNA-96™ Soil Microbe DNA Kit quickly and efficiently isolates humic-free, PCR-ready genomic DNA from soil samples in approximately 50 minutes. Gram(+/-) bacteria, fungi, plant and animal tissues, algae, spores and many other members of a soil population including clay, sandy, silty, peaty, chalky, and loamy soils, are efficiently lysed in approximately 60 seconds with the FastPrep-96™ Instrument. Research use only. Key applications include isolation of PCR-Ready Genomic DNA from Soil Samples.

FastDNA™ Spin Kit

Brand:

MP Biomedicals

The FastDNA™ SPIN Kit, an updated version of the well-known FastDNA™ Kit, is used with any FastPrep® Instrument to lyse and subsequently isolate DNA from up to 200 mg of almost any sample in less than 30 minutes. Features: Isolation of genomic DNA from plants, animals, bacteria, yeast, algae, and fungi using a silica spin fi lter method: Rapid and reproducible sample lysis with the FastPrep®-24 or FastPrep® FP120 Instrument, Lyse and isolate DNA in less than 30 minutes from a variety of sample types, Flexible format eliminates need for separate DNA isolation kits, No hazardous organic reagents required, SPIN Filters are included to streamLine silica handling. Research Use Only. Key applications include DNA extraction and purification from virtually and sample.

FastDNA™ Spin Kit for Soil

Brand:

MP Biomedicals

The FastDNA® SPIN Kit is designed for use with any FastPrep® Instrument, plant and animal tissues, bacteria, algae, fungi spores and other members of a soil population are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by simultaneous impaction with lysing matrix particles. Features: Isolation of DNA from cells present in soil or other environmental samples, Rapid and reproducible sample lysis with the FastPrep®-24 or FastPrep® FP120 Instrument, Easily isolate DNA from a variety of organisms in many different types of soil, Lyse and isolate DNA in less than 60 minutes, No hazardous organic reagents required. Research Use Only. Key applications include isolation of bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.

Monarch DNA Gel Extraction Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify DNA from agarose gels with high yields.

  • Elute in as little as 6 μl.
  • Prevent buffer retention and salt carry-over with optimized column design.
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol.
  • Buffers and columns available separately.
  • Significantly less plastic used when compared with other kits.
  • Responsibly-sourced and recyclable packaging.

** Tips for using Monarch DNA Gel Extraction kit

Monarch PCR and DNA Cleanup Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify high-quality DNA from PCR and other enzymatic reactions.

  • Elute in as little as 6 μl.
  • Prevent buffer retention and salt carry-over with optimized column design.
  • Save time with fast, user-friendly protocols.
  • No need to monitor pH.
  • Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments.
  • Buffers and columns available separately.
  • Significantly less plastic used when compared with other kits.
  • Responsibly-sourced and recyclable packaging.

** Tips for using Monarch PCR and DNA Cleanup Kit

Monarch Plasmid Miniprep Kit

Brand:

New England Biolabs

The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of up to 20 μg of high quality plasmid DNA.

  • Elute in as little as 30 μl
  • Prevent buffer retention and salt carry-over with optimized column design
  • Reduce hands on time with faster protocols and less spin time
  • Monitor completion of certain steps using colored buffer system
  • No need to add RNase before starting
  • Easily label columns using tab and frosted surfaces
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging
  • No hazardous materials fees

** Tips for using Monarch Plasmid Miniprep Kit

Monarch RNA Cleanup Kit (10 µg), NEB

Brand:

New England Biolabs

The Monarch RNA Cleanup Kit (10 µg) enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
  • Elute in ≥ 6 µl for concentrated RNA
  • 70-100% RNA recovery, even with inputs < 20 ng
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
  • Simplified workflow with a single wash buffer
  • Unique column design prevents buffer carryover and elution of silica particulates
  • Columns and buffers are available separately
  • Purified RNA is ready for use in a wide variety of downstream applications

** Tips for using Monarch RNA Cleanup Kit

Monarch RNA Cleanup Kit (500 µg), NEB

Brand:

New England Biolabs

The Monarch RNA Cleanup Kit (500 µg), NEB enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.

  • Ideal for purification of synthesized RNA following high-yield in vitro transcription reactions (alternative to MEGAclear™)
  • Optimized for the cleanup of RNA after enzymatic treatments including DNase I, Proteinase K, labeling and capping
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Elute in ≥ 50 µl for concentrated RNA 70-100% RNA recovery
  • Unique column design prevents buffer carryover and elution of silica particulates
  • Simplified workflow with a single wash buffer
  • Columns and buffers are available separately
  • Purified RNA is ready for use in a wide variety of downstream applications, including microinjection and transfection.

** Tips for using Monarch RNA Cleanup Kit

Monarch® DNA/RNA Protection Reagent

Brand:

New England Biolabs

  • Preserves and stabilizes RNA and DNA
  • A component of the Monarch® Total RNA Miniprep Kit (NEB #T2010)
  • Supplied as a 2X concentrate
  • Supplied in 2 bottles, each containing 28 ml

Monarch® Genomic DNA Purification Kit, NEB

Brand:

New England Biolabs

Quickly and easily purify high quality, high molecular weight gDNA from multiple sample types, all with one kit!

  • For use with a broad range of sample types, such as cells, blood, and tissues (including fatty and fibrous tissues)
  • Also works with tough to lyse samples (e.g., bacteria, yeast)
  • Suitable for clinically-relevant samples (e.g., saliva, cheek)
  • Obtain excellent yields of highly-pure DNA
  • Extremely low residual RNA contamination (typically <1%)
  • Isolates high molecular weight gDNA (peak size typically ≥ 50 kb)
  • Use DNA directly in downstream applications, including PCR, qPCR and NGS
  • Fast, user-friendly protocols with short lysis times and minimal hands-on time
  • Can also be used to clean up previously extracted gDNA
  • Includes RNase A
  • Kit components available separately

** Tips on how to maximize DNA yield using Monarch Genomic DNA Purification kit

Monarch® HMW DNA Extraction Kit for Cells & Blood

Brand:

New England Biolabs

  • For the extraction of HMW DNA from cells and blood
  • Isolate DNA into the Mb size range
  • Easily tune the size of isolated DNA by adjusting the agitation speed during lysis
  • Fast, user-friendly workflows (30 minutes for cells, 60 minutes for blood)
  • Obtain best-in-class yields of highly-pure and intact DNA
  • Effective RNA removal
  • Rapid and efficient elution
  • Includes RNase A and Proteinase K
  • Ideal for long-read sequencing applications

Monarch® HMW DNA Extraction Kit for Tissue

Brand:

New England Biolabs

  • For the extraction of HMW DNA from a variety of tissues, bacteria and other samples (yeast, insect, amphibian)
  • Isolate DNA into the Mb size range from soft organ tissues and bacteria
  • For some samples, the size of isolated DNA can be tuned by adjusting the agitation speed during lysis
  • Fast, user-friendly workflows (90 minutes for tissues and bacteria)
  • Obtain excellent yields of highly-pure and intact DNA
  • Rapid and efficient elution
  • Ideal for long-read sequencing applications
  • Includes RNase A and Proteinase K, as well as microtube pestles for convenient sample homogenization

Monarch® RNA Cleanup Kit (50µg), NEB

Brand:

New England Biolabs

The Monarch RNA Cleanup Kit (50 µg), NEB enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labelling, capping or in vitro transcription (IVT)
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Elute in ≥ 20 µl for concentrated RNA
  • 70-100% RNA recovery
  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
  • Simplified workflow with a single wash buffer
  • Unique column design prevents buffer carryover and elution of silica particulates
  • Columns and buffers are available separately
  • Purified RNA is ready for use in a wide variety of downstream applications, including transfection

** Tips for using Monarch RNA Cleanup Kit

Monarch® Total RNA Miniprep Kit

Brand:

New England Biolabs

Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types – all with Monarch Total RNA Miniprep kit!

  • For use with blood, cells and tissues
  • Also works with tough to lyse samples (bacteria, yeast, plant)
  • Effectively purifies total RNA of all sizes, including small RNAs >20 nt
  • Efficient genomic DNA removal (column and DNase I-based)
  • Contains Proteinase K for processing of tissues and blood samples
  • Includes RNA Protection Reagent for sample preservation
  • Excellent value
  • Kit components available separately

** You may also interest in RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit.

** Tips for using Monarch RNA Cleanup Kits 

RNAlater® (Sigma)

Brand:

Sigma

Description

Synonyms: RNA preservation solution, RNA protection solution, RNA protector, RNA stabilizer, RNA storage solution, RNA transportation solution

RNAlater® is an RNA stabilization solution that is designed to quickly permeate and stabilize RNA in tissues and cells after collection. It allows for long-term storage of samples at different temperatures, including room temperature, without compromising RNA integrity. It preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term.