Fraser Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Fraser Supplement is used for the isolation of Listeria species.
- Add to Fraser Broth Base, Part No. CM0895B
- Each vial supplements 500mL of medium
Fraser Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Ferric ammonium citrate | 0.25g | 0.5g |
| Nalidixic acid | 10.0mg | 20.0mg |
| Acriflavine hydrochloride | 12.5mg | 25.0mg |
Fraser Broth, Oxoid Preparation:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.
Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.
Fy100 Framycetin
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Thermo ScientificTM OxoidTM
G C Agar Base 500g
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Thermo ScientificTM OxoidTM
Selectively isolate Neisseria species with Thermo Scientific™ Oxoid™ GC Agar Base (Dehydrated). GC Agar Base has been formulated to include Special Peptone LP0072 which is a mixture of meat and plant enzymatic digests. The presence of starch in the medium ensures that toxic metabolites produced by Neisseria are absorbed. Phosphate buffers are included to prevent changes in pH due to amine production that would affect the survival of the organism
GC Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Special peptone | 15.0 |
| Corn starch | 1.0 |
| Sodium chloride | 5.0 |
| Dipotassium hydrogen phosphate | 4.0 |
| Potassium dihydrogen phosphate | 1.0 |
| Agar | 10.0 |
| pH 7.2 ± 0.2 @ 25°C |
G-N Anaerobe Selective Supplement
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Oxoid G-N Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.
G-N Anaerobe Selective Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Haemin | 2.5mg | 5.0mg |
| Menadione | 0.25mg | 0.5mg |
| Sodium succinate | 1.25g | 2.5g |
| Nalidixic acid | 5.0mg | 10.0mg |
| Vancomycin | 1.25mg | 2.5mg |
Selective Medium for Gram-Negative Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of G-N Anaerobe Supplement (SR0108) rehydrated as directed, together with 25ml defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.
GBS Agar Base Islam 500g
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Thermo ScientificTM OxoidTM
This product has been discontinued. Please contact us for more information.
Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.
GBS Agar Base (Islam), Oxoid Composition
| Typical Formula* | gm/litre |
| Proteose peptone | 23.0 |
| Soluble starch | 5.0 |
| Sodium dihydrogen phosphate | 1.5 |
| Di-sodium hydrogen phosphate | 5.75 |
| Agar | 10.0 |
| pH 7.5 ± 0.1 @ 25°C |
GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.
Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.
GDH-BASIC Gel Documentation hood
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Cleaver Scientific
The GDH-BASIC is a low cost, simple to use gel documentation hood for use with a mobile phone, reduces background from ambient light when using blue light transilluminators. Simply place the hood over your.Blue light Transilluminator and use your phone to zoom in and take an image. Then transfer the images from your phone to your analysis software. Ideal for education use or where low costs are important!
Gel Loading Dye, Purple (6X), no SDS – 4.0 ml, NEB
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New England Biolabs
Gel Loading Dye, Purple (6X), NEB is the premier purple loading dye for sharp, tight bands.
- No UV shadow
- Contains Ficoll® for brighter, tighter bands
- Contains EDTA to stop enzymatic reactions
- Compatible with agarose and non-denaturing polyacrylamide gels
- No UV shadow allows for publication grade images
- Brighter, sharper bands vs. glycerol-based loading dyes
- Stops enzyme reactions
Gelatin Porcine Skin 90-110 Bloom
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Thermo ScientificTM OxoidTM
Oxoid Gelatin Bacteriological is a special bacteriological grade readily soluble in water to give a clear solution free from sulphite and preservatives.
- Firm gel obtained from 15% solution
Gellan Gum, Plant Cell Technology
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Plant Cell Technology
Gellan gum also known as Phytagel, Gelzan, Gelrite, or Applied gel is a water soluble anionic polysaccharide produced by fermentation of a carbohydrate by pseudomonas Elodea, purified, dried, and powdered.
Gellan gum is ideal for preparation of nutrient media for in plant tissue culture work and is a substitute for agar.Gels produced by gellan gum are clearer in comparison to agar however the gel is more rigid and brittle than agar gel. Gellan gum requires roughly half the amount of agar that is needed. Gellan gum melts at 80°C– 100°C and gels between 10°C-50°C.Gellan gum also known as Phytagel, Gelzan, Gelrite, or Applied gel is a water soluble anionic polysaccharide produced by fermentation of a carbohydrate by pseudomonas Elodea, purified, dried, and powdered.
Gellan gum is ideal for preparation of nutrient media for in plant tissue culture work and is a substitute for agar.Gels produced by gellan gum are clearer in comparison to agar however the gel is more rigid and brittle than agar gel. Gellan gum requires roughly half the amount of agar that is needed. Gellan gum melts at 80°C– 100°C and gels between 10°C-50°C.
Gel Strength: >900 GM/CM2
Application:
Gellan Gum is used as microbial solid culturing media. The substance is often used in doses ranging between 0.2% to 0.4% (w/v) to solidify plant tissue culture media. PPM can be mixed in when creating Gellan Gum media
gelONE Gel Documentation System with built in touch screen
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Cleaver Scientific
The gelONE is the ultimate in simplicity, with automated acquisition through an intuitive touch screen interface. Compatible with both traditional DNA stains and safe stains, the gelONE is suitable for any lab. 5 Megapixel camera gives excellent low light sensitivity for clear resolution of closely spaced DNA bands and ensures even low levels of fluorescence are detected. The 20 x 24 cm imaging area means even large gels can be imaged with ease. Images are captured using the built in software, and saved direct to USB memory stocks for transfer to PC. The geneQUANT advanced image analysis package is included with all systems license free.
Key application includes:
DNA – Image Agarose gels stained with EtBr, SYBR stains and runSAFE
AutoRads – the gelONE™ high resolution camera makes it ideal for capturing Autorads
Visible Light – Image Coomassie stained gels, tissues, slides and films
Product Includes: gelONE Imaging System, UV Transilluminator, UV filter, software USB with gelQUANT software, power cable and Instruction manuals
Please contact us for pricing and availability.
GelStarTM Nucleic Acid Gel Stain, 10,000X (2 x 250 uL)
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Lonza
GelStarTM Nucleic Acid Gel Stain is a highly sensitive fluorescent stain for detecting both DNA and RNA. You can choose to either add GelStarTM Stain to your agarose solution prior to casting or to post stain your gels. GelStarTM Nucleic Acid Gel Stain exhibits exceptional signal-to-noise ratio with minimal background. Maximum sensitivity, detect as little as 20 pg of dsDNA or 3 ng of RNA.
Benefits
Applications
Gentamicin sulfate salt – Sigma-Aldrich
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Sigma
Description
Cas Number : 1045-41-0
Synonyms : Gentiomycin C
Chemical structure: aminoglycoside
Gentamicin, also known as gentiomycin C, is an aminoglycoside, which is naturally produced by Gram-negative bacteria. This antibiotic functions by preventing protein synthesis.[1] Gentamicin sulfate prevents the growth of both Gram positive and Gram negative bacteria, in vivo and in vitro. In tissue culture, gentamicin sulfate is used to inhibit the growth of various strains of mycoplasma.[2].
Gentamicin Supplement
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Thermo ScientificTM OxoidTM
Oxoid Gentamicin Selective Supplement (256mg/vial) is used for the isolation of Vancomycin-Resistant Enterococci (VRE) and High Level Aminoglycoside-Resistant Enterococci (HLARE) from clinical specimens.
- Add to VRE Agar Base, Part No. CM0985B
- Each vial supplements 500mL of medium
Gentamicin Selective Supplement, Oxoid Composition
| Vial Contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
| Gentamicin | 256.0mg | 512.0mg |
Storage conditions and Shelf life
Gentamicin Supplement SR0185 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.
Gentamycin 1024 M.I.C.E. (10 Pack)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Gentamycin M.I.C.Evaluator Strips (M.I.C.E.™).
Gentamycin 1024 M.I.C.E. (50 Pack)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Gentamycin M.I.C.Evaluator Strips (M.I.C.E.™).
Gentamycin 256 M.I.C.E. (10 Pack)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Gentamycin M.I.C.Evaluator Strips (M.I.C.E.™).
Gentamycin 256 M.I.C.E. (50 Pack)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Gentamycin M.I.C.Evaluator Strips (M.I.C.E.™).
Gibberellin 80% gibberellin A3 basis (TLC) (Sigma-Aldrich)
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Sigma
Description
Formula : C19H22O6
Molecular weight : 346.37 g/mol
Synonym : GA3
A “classical” ubiquitous plant hormone, with diverse effects, such as stilumating stem and root growth, and stimulating seed germination.
Giemsa’s Azur Eosin Methylene Blue Solution (Merck)
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Merck
**This product has been discontinued, please contact us for more information.
Description
The Giemsa’s Azure Eosin Methylene Blue solution can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. The Giemsa stain can also be used to detect Helicobacter pylori in gastric tissue biopsies. The specimen material will show cell nuclei stained in various shades of blue, while other structures will appear in various red types. It is an IVD product and CE registered.
Application
For microscopy. For the staining of blood and bone-marrow smears, paraffin sections and clinical-cytological specimens
Giolitti-Cantoni Broth 500g
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Thermo ScientificTM OxoidTM
An anaerobic enrichment broth for Staphylococcus aureus.
Giolitti-Cantoni Broth, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 10.0 |
| `Lab-Lemco’ powder | 5.0 |
| Yeast extract | 5.0 |
| Lithium chloride | 5.0 |
| Mannitol | 20.0 |
| Sodium chloride | 5.0 |
| Glycine | 1.2 |
| Sodium pyruvate | 3.0 |
| pH 6.9 ± 0.2 @ 25°C |
Giolitti-Cantoni Broth, Oxoid Preparation:
Suspend 54.2g in one litre of distilled water and heat gently to dissolve. Dispense 19ml amounts into 20 x 200mm test tubes and sterilise by autoclaving at 121°C for 15 minutes. Cool rapidly then aseptically add to each tube 0.3ml of a sterile solution of Potassium tellurite 3.5% SR0030.
The medium requires the addition of a 3.5% solution of Potassium tellurite when there is a direct addition of 1g of the sample to 19mls of broth. This level of Potassium tellurite is necessary to suppress the high numbers of contaminating organisms that could be expected.
The use of a diluted solution of Potassium tellurite is applicable when a 1 in 10 dilution of the food sample is carried out. In such cases the SR0030 should first be diluted 1 in 10 with sterile distilled water.
The addition of 0.1% Tween 80 can be recommended in order to improve recovery of heat injured Staphylococcus aureus cells e.g. from milk powder. 1g of Tween 80 should be added to 1 litre of CM0523 prior to autoclaving.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Glutathione, Certified Reference Material (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 70-18-8
Chemical Formula: H2NCH(CO2H)CH2CH2CONHCH(CH2SH)CONHCH2CO2H
Molar Mass: 307.32g/mol
Synonyms: γ-L-Glutamyl-L-cysteinyl-glycine, L-Glutathione reduced, GSH
Amino Acid Sequence
γ-Glu-Cys-Gly
General description
Glutathione (GSH) is the most important nonprotein thiol widely distributed in animal tissues, plants, and microorganisms. GSH is also a key determinant of redox signalling and protection against oxidative stress.
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.
Application
HPLC: suitable
Gas Chromatography (GC): suitable
These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
May be used at 5-10 mM to elute glutathione S-transferase (GST) from glutathione agarose.
Biochem/physiol Actions
Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.
Glycerol (Glycerine) AR, 2.5L
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Chemiz
Description
Synonyms: Glycerine
Formula: C3H8O3
Analytical reagent grade glycerol for various laboratory uses including gel formation for polyacrylamide gel, altering density of liquids and prevention of freezing for certain samples when stored.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Glycerol, For Molecular Biology, ≥99.0% (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 56-81-5
Chemical Formula: HOCH2CH(OH)CH2OH
Molar Mass: 92.09 g/mol
Synonyms: 1,2,3-Propanetriol, Glycerin
General description
Glycerol is odourless, colourless, viscous in nature, and exists as a sweet-tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact on the environment.
Application
Glycerol has been used as:
• A supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
• A fuel during designing enzymatic biofuel cell.
• A liquid composite matrix with 4-HCCA and 3-aminoquinoline for analysis of neutral and acidic glycans.
• A matrix for fast atom bombardment MS.
• May be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Glycine AR, 500g
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Chemiz
Description
Synonyms: 2-Aminoacetic acid
Formula: C2H5NO2
Glycine is an important component for some solution used in polyacrylamide gel electrophoresis method in protein analysis, acting as buffer to maintain pH level to prevent damage to the sample during analysis.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
