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Detect salmonellae including Salmonella arizonae with the diagnostic Thermo Scientific™ Oxoid™ Lysine Iron Agar (Dehydrated). This differential medium detects salmonellae (including lactose fermenting Salmonella arizonae) by lysine decarboxylase activity and H₂S production.

Lysine Iron Agar, Oxoid Composition

Lysine Iron Agar, Oxoid Preparation:
Suspend 34g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into tubes and sterilise by autoclaving at 121° C for 15 minutes. Cool the tubes in an inclined position to form slants with deep butts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8° C.

Isolate and enumerate wild yeasts encountered in brewing while suppressing pitching yeasts with Thermo Scientific™ Oxoid™ Lysine Medium (Dehydrated). This complex, synthetic medium is based on the original formula of Morris & Eddy which utilizes the susceptibility of pitching yeasts to lysine to differentiate it from the wild yeast.

Lysine Medium, Oxoid Composition

Lysine Medium, Oxoid Preparation:
Suspend 6.6g in 100ml distilled water containing 1.0ml Potassium lactate SR0037. Bring to the boil to dissolve completely. Agitate frequently to prevent superheating. Cool to 50°C and add 0.1ml of lactic acid 10% SR0021 to adjust to pH 4.8 ± 0.2. Dispense into Petri dishes and remove surface moisture by drying at 37°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Membrane Endo Agar LES (m Endo AgarLES) is an APHA recommended medium for the confirmation of coliforms.
• Also available Basic Fuchsin Indicator, Part No. BR0050A.

Membrane Endo Agar LES, Oxoid Composition

Membrane Endo Agar LES, Oxoid Preparation:
Suspend 45 grams in 1 litre of distilled water. Add 12ml of a 10% w/v solution of Basic Fuchsin. Heat gently with frequent agitation until the medium boils. DO NOT AUTOCLAVE. Cool to 45°C and dispense into 50-60 mm dishes in 4 ml volumes. For larger dishes use sufficient medium to give an equivalent depth (approx. 1.5 mm).
Plates should be protected from light and may be stored for up to two weeks in the refrigerator.

Storage conditions and Shelf life
Store the dehydrated medium below 25°C and use before the expiry date on the label.
Store the prepared medium in the dark and at 2-8°C.

Isolate and enumerate lactic acid bacteria from food with Thermo Scientific™ Oxoid™ MRS Broth (DeMan, Rogosa and Sharpe) (Dehydrated). This non-selective medium is designed to support profuse growth of lactic acid bacteria.

MRS Broth (DeMan, Rogosa and Sharpe), Oxoid Composition

MRS Broth (DeMan, Rogosa and Sharpe), Oxoid Preparation:
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

M17 Agar, Oxoid is used for the improved growth of Lactic Streptococci and their bacteriophages and the selective enumeration of Streptococcus thermophilus from yogurt.

M17 Agar, Oxoid Composition

M17 Agar, Oxoid Preparation:
Suspend 48.25g in 950ml of distilled water and bring gently to the boil. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2µm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Cultivate lactic streptococci and their bacteriophages with Thermo Scientific™ Oxoid™ M17 Broth (Dehydrated). The use of M17 Broth in conjunction with M17 Agar is recommended by Terzaghi and Sandine for bacteriophage assays.

M17 Broth, Oxoid Composition

M17 Broth, Oxoid Preparation:
Suspend 37.25g in 950ml of distilled water and bring gently to the boil. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2mm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.