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Selectively isolate Salmonella species from food samples with Thermo Scientific™ Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn) for food testing in compliance with ISO 6579:2002.

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Composition

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Preparation:
Suspend 89.5g of MKTTn in 1 litre of distilled water, mix well and bring to the boil. Cool to below 45°C. Immediately before use add 20ml of iodine-iodide solution prepared by dissolving 25g of potassium iodide in 10ml of water, adding 20g of iodine and then diluting to 100ml with sterile water. Also add the contents of four vials of Oxoid Novobiocin Selective Supplement SR0181 reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at room temperature. The prepared medium in its final form containing iodine/iodide solution + Novobiocin Selective Supplement SR0181 should be used immediately.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between Escherichia coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies

Brilliance E. coli/coliform Selective Agar, Oxoid Composition

Brilliance E. coli/coliform Selective Agar, Oxoid Preparation:
Suspend 28.1g of Brilliance E. coli/coliform Selective Agar in 1 litre of distilled water. Bring the medium gently to the boil, to dissolve completely. Either pour the medium into sterile Petri dishes or keep molten at 45ºC for pour plate technique.

Storage conditions and Shelf life
Dehydrated medium: store tightly capped in the original container at 10-30°C
Prepared medium will be stable for up to 2 weeks when stored at 2-8ºC

Brilliance Bacillus Cereus Agar Base, Oxoid is a chromogenic medium for the isolation and differentiation of Bacillus cereus from food samples.

• Also available Chromogenic Bacillus Cereus Selective Supplement, Part No. SR0230E.

Brilliance Bacillus Cereus Agar Base, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

Precautions
X-glucopyranoside negative B. cereus strains may present white colonies on this medium due to poor or no utilisation of the chromogen.

Oxoid Heart Infusion Broth is a highly nutritious liquid medium recommended for the cultivation of fastidious microorganisms.

Heart Infusion Broth, Oxoid Composition

Heart Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121ºC for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium in the dark below 20°C.

Precautions
Heart Infusion Broth should only be used for in vitro diagnostic purposes.

Detect, enumerate and differentiate Escherichia coli and other coliforms by single membrane filtration with Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated). The presence of lauryl sulphate and lactose with phenol red allow the selective isolation and differentiation of Escherichia coli and coliforms respectively.

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Composition

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium may be stored for up to 1 week at 2-8°C.

Enrich and recover Vibrio species from food, water, and clinical samples with Thermo Scientific™ Oxoid™ Alkaline Peptone Water. This medium is selective by exploiting the ability of Vibrio species to tolerate alkaline conditions and high sodium chloride levels. The peptone content provides the conditions for growth and recovery of Vibrio species, supplying vitamins, minerals, and nitrogen in the form of amino acids.

Alkaline Saline Peptone Water, Oxoid Composition

Alkaline Saline Peptone Water, Oxoid Preparation:

Add 30g to 1 litre of distilled water. Mix well, distribute into final containers and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium may be stored for up to 1 month at room temperature.

Numerate all culturable microorganisms from water using colony count technique with Thermo Scientific™ Oxoid™ Water Plate Count Agar (Dehydrated). This nutritious, non-selective medium conforms to ISO 6222: 1999 (E) Water quality ― Enumeration of culturable microorganisms ― Colony count by inoculation in a nutrient agar culture medium1.

Water Plate Count Agar (Dehydrated), Oxoid Composition

Water Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 24g of Water Plate Count Agar (ISO) in 1 litre of distilled water and bring gently to the boil to dissolve. If desired, distribute volumes of 15ml to 20ml in tubes, bottles or other containers. Sterilise by autoclaving at 121°C for 15 minutes. After sterilisation, allow to cool and maintain at 45 ± 1°C using a water bath.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

• Oxoid Oxacillin Resistance Screening Agar Base (ORSAB) is a medium for screening clinical samples for the presence of oxacillin-resistant Staphylococci.
• Also available ORSAB Selective Supplement, Part No. SR0195E.

Oxacillin Resistance Screening Agar Base (ORSAB), Oxoid Composition

Oxacillin Resistance Screening Agar, Oxoid Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Chromogenic Salmonella Agar Base is a selective chromogenic medium for the presumptive identification of Salmonella spp. from clinical and food samples.
• Also available Salmonella Selective Supplement, Part No. SR0194E and Brilliance™ Salmonella Agar Base, Part No. CM1092B.

Chromogenic Salmonella Agar Base, Oxoid Composition

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 6 weeks at 2-8°C.

• Oxoid Cefixime Rhamnose Sorbitol MacConkey Agar Base (CR-SMAC) is a selective and differential medium containing rhamnose and cefixime for the isolation of E. coli O157.
• Also available Cefixime Supplement, Part No. SR0191E.

CR-SMAC Agar Base, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Brilliance Candida Agar Base is a chromogenic medium for the isolation and differentiation of clinically important Candida spp., including C. albicans.
• Also available Chromogenic Candida Agar Selective Supplement, Part No. SR0231E.

Brilliance Candida Agar Base, Oxoid Composition

Brilliance Candida Agar, Oxoid Preparation:
Suspend 15.6g in 500ml of distilled water. Add 1 vial of Brilliance Candida Selective Supplement reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool the medium to 45°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Brilliance Candida Agar Base must be stored tightly capped in the original container at 10-30°C. When stored as directed the unopened products will remain stable until the expiry date printed on the containers.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Isolate Burkholderia cepacia from respiratory secretions of patients with cystic fibrosis with Thermo Scientific™ Oxoid™ Burkholderia Cepacia Agar Base, which is also used for routine testing of non-sterile inorganic salt solutions in hospital environments. The slower growing B. cepacia can be missed on conventional media, such as blood or MacConkey Agar, due to overgrowth caused by other faster growing organisms found in the respiratory tract of CF patients, such as mucoid Klebsiella species,
Pseudomonas aeruginosa and Staphylococcus species.This may lead to the infection being missed or wrongly diagnosed.

Burkholderia Cepacia Agar Base, Oxoid Composition

Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

• Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples.
• Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.

Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition

(M-CP) Agar Base, Oxoid Preparation:

Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to 5 days at 2-8°C in the dark.

• Oxoid EC Broth, Reduced Bile Salts is a selective enrichment broth for the growth of Escherichia coli O157 from food and environmental samples.
• Also available Novobiocin Selective Supplement, Part No. SR0181E.

EC Broth (Reduced Bile Salts), Oxoid Composition

EC Broth (Reduced Bile Salts), Oxoid Preparation:
Dissolve 18.3g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool the EC Broth (Reduced Bile Salts) to 50°C and aseptically add the contents of 1 vial of Novobiocin Supplement SR0181, reconsituted as directed. Dispense into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
When stored as directed, the supplement remains stable until the expiry date printed on the label.

Oxoid VRE Agar Base (Vancomycin Resistant Enterococci Agar Base) is used for selective isolation of vancomycin-resistant enterococci and high level aminoglycoside-resistant enterococci (HLARE) from clinical samples.Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Agar Base, Oxoid Composition

VRE Agar, Oxoid Preparation:
Suspend 42.6g of VRE Agar Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121° C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then Mix agar well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.
Warning: Sodium azide is harmful if swallowed, please take all necessary precautions.

This product has been discontinued. Please contact us for more information.

• Oxoid VRE Broth Base (Vancomycin Resistant Enterococci Broth Base) is for selective isolation of vancomycin-resistant enterococci and high Level aminoglycoside-resistant enterococci.
• Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Broth Base, Oxoid Composition

VRE Broth, Oxoid Preparation:
Suspend 37.0g of VRE Broth Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121°C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then mix well and distribute into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.

Bolton Broth Base, Oxoid Composition

Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.

Carry out microbial limit testing by harmonized methodology of pharmaceutical products in accordance with USP/EP/BP/JP with Thermo Scientific™ Oxoid™ Buffered Sodium Chloride Peptone Solution (Dehydrated). The combination of phosphate buffer, sodium chloride and peptone increases the viability of sensitive microorganisms by providing an isotonic environment. This is of particular importance when trying to recover cells that may be stressed or sensitive to osmotic pressure.

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Composition

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Preparation:
Suspend 14.63g of Sodium Chloride Peptone Broth (Buffered) in 1 litre of water (purified as required). If required, add 1-10ml Tween® 20 or 80 per litre of medium. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared media at room temperature.

• Oxoid Sorbitol MacConkey Agar w/BCIG is a selective medium containing the chromogenic substrate BCIG.
• Can be used with C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar w/BCIG, Oxoid Composition

Sorbitol MacConkey Agar w/BCIG, Oxoid Preparation:
Suspend 51.6g of SMAC with BCIG in 1 litre of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Lauryl Tryptose Broth w/MUG is used for the presumptive identification of E. coli.

Lauryl Tryptose Broth with MUG, Oxoid Composition

Lauryl Tryptose Broth, Oxoid Preparation:
Suspend 35.65g of Lauryl Tryptose Broth with MUG in 1 litre of distilled water. Warm to 40°C. Mix well to dissolve and distribute into final containers holding inverted Durham tubes. (Please be aware of the special glassware requirements when using ultra-violet light.). Sterilise by autoclaving at 121° C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Oxoid EC Broth with MUG is used for the isolation and presumptive identification of E. coli.

EC Broth with MUG, Oxoid Composition

EC Broth with MUG, Oxoid Preparation:
Suspend 37g of EC Broth with MUG in 1 litre of distilled water. Mix well to dissolve and distribute into final containers containing inverted Durham tubes. Sterilise by autoclaving at 121° C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Ensure that the glass tubes used for the preparation of the medium do not auto fluoresce and take the relevant safety precautions when using a UV lamp.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give a positive result in this test.
Other enteric organisms e.g. some Shigella species are also glucuronidase positive.

Oxoid Violet Red Bile Agar w/MUG is used for the presumptive identification of E. coli.

Violet Red Bile Agar with MUG, Oxoid Composition

Violet Red Bile Agar with MUG, Oxoid Preparation:
Suspend 38.6g of VRBA with MUG in 1 litre of distilled water. Bring to the boil to dissolve completely. No further sterilisation is necessary or desirable. Mix well before pouring into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

• Oxoid Anaerobe Basal Agar is used for growth of fastidious and other anaerobes.
• Also available N-S Anaerobe Selective Supplement, Part No. SR0107B, for use for nonsporforming anaerobes and G-N Anaerobe Selective Supplement, Part No. SR0108B, for use for gram-negative anaerobes.

Anaerobe Basal Agar, Oxoid Composition

Anaerobe Basal Agar, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium for upto 3 weeks at 2-8°C.