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Selectively detect Escherichia coli from food and environmental samples with Thermo Scientific™ Oxoid™ EC Broth (Dehydrated). EC Broth is a selective medium for the differentiation of fecal coliforms and the confirmatory test for Escherichia coli from food and environmental samples. It is also suitable for the enumeration of presumptive E. coli in milk and milk products using the most probable number technique.

EC Broth, Oxoid Composition

EC Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Dispense into final containers and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 4 weeks.

• Oxoid Aeromonas Medium Base (Ryan) is a selective differential medium for the isolation of Aeromonas hydrophila.
• Also available Ampicillin Selective Supplement, Part No. SR0136E.

Aeromonas Medium Base (Ryan), Oxoid Composition

Aeromonas Medium Base (Ryan), Oxoid Preparation:
Suspend 29.5g in 500ml of distilled water. Bring gently to the boil. DO NOT AUTOCLAVE. Cool to 50°C and aseptically add one vial of Ampicillin Selective Supplement SR0136 reconsituted as directed. Mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium 2-8°C.

Precautions
Although Aeromonas and Plesiomonas spp will grow on the medium if ampicillin is omitted, it will be more difficult to distinguish them from the other organisms present on the plate. Suspected colonies of Aeromonas spp must be confirmed by biochemical tests.

Cultivate lactic streptococci and their bacteriophages with Thermo Scientific™ Oxoid™ M17 Broth (Dehydrated). The use of M17 Broth in conjunction with M17 Agar is recommended by Terzaghi and Sandine for bacteriophage assays.

M17 Broth, Oxoid Composition

M17 Broth, Oxoid Preparation:
Suspend 37.25g in 950ml of distilled water and bring gently to the boil. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2mm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Sorbitol MacConkey Agar is a selective differential medium for the detection of E. coli O157.
• Also available C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar Base, Oxoid Composition

Sorbitol MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C. Pour into sterile Petri dishes.

If required, reconstitute one vial of C-T supplement per 500ml medium, according to the instructions in the product leaflet. Aseptically add this to cooled medium before pouring into sterile Petri dishes

Storage conditions and Shelf life
Dehydrated Sorbitol MaConkey Agar must be stored tightly capped in the original container at 10-30ºC.

Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.

Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Oxoid Tergitol 7 Agar is used for the enumeration and identification of coliforms.

Tergitol 7 Agar, Oxoid Composition

TTC Solution (SR0148) is supplied as 2ml of filtered 0.125% aqueous solution of tri-phenyltetrazolium chloride (TTC).

Tergitol 7 Agar, Oxoid Preparation:
Suspend 54.15g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense in 100ml volumes and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the contents of 1 vial of SR0148. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Tergitol-7 Agar is designed for early detection of Escherichia coli i.e. 6-10 hours incubation.
Incubation at 44°C has been recommended.

M17 Agar, Oxoid is used for the improved growth of Lactic Streptococci and their bacteriophages and the selective enumeration of Streptococcus thermophilus from yogurt.

M17 Agar, Oxoid Composition

M17 Agar, Oxoid Preparation:
Suspend 48.25g in 950ml of distilled water and bring gently to the boil. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2µm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid MLCB Agar is used for the selective isolation of Salmonella spp., not S.Typhi or S. Paratyphi A.

MLCB Agar, Oxoid Composition

MLCB Agar, Oxoid Preparation:
Suspend 49.0g in 1 litre of distilled water. Mix and bring gently to the boil with frequent agitation to dissolve the medium completely. Cool to 50°C and pour approximately 20ml into sterile Petri dishes.
DO NOT AUTOCLAVE OR OVERHEAT.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The identity of colonies presumed from their appearance to be Salmonella spp. must be confirmed by biochemical and serological testing. In common with other enteric media care must be taken to ensure the purity of colonies taken for further testing as organisms that are inhibited from developing into colonies remain viable and may accidentally be picked on sub-culture.

• Oxoid Raka-Ray Agar Base is a medium for the isolation of lactic acid bacteria in beer and brewing processes.
• Requires supplementation with phenylethanol, sorbitan mono-oleate, and cycloheximide Part No. SR0222C.

Raka-Ray Agar Base, Oxoid Composition

Raka-Ray Agar, Oxoid Preparation:
Suspend 77.1g in 1 litre of distilled water. Add 10ml of sorbitan mono-oleate and 7ml of 0.1% Cycloheximide Solution (SR0222). Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 3g of phenylethanol. Pour into sterile Petri dishes or distribute into 4ml volumes held at 55°C if the overlay technique is to be used.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Although the concentration of cycloheximide in the medium is below toxic levels, precautions should be observed as detailed under HAZARDS section.

This product has been discontinued. Please contact us for more information.

Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.

GBS Agar Base (Islam), Oxoid Composition

GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.

Isolate Campylobacter jejuni, Campylobacter coli and Compylobacter laridis using Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated) when used with CCDA Selective Supplement SR0155. Campylobacter Blood-Free Selective Agar Base is based on the original formulation described by Bolton et.al which was developed to replace blood with charcoal, ferrous sulphate and sodium pyruvate. For improved selectivity cephazolin in the formulation was replaced by cefoperazone as a selective agent.

Campylobacter Blood-Free Selective Agar Base, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Achieve maximum recovery of microorganisms during dilution with this protective, isotonic Thermo Scientific™ Oxoid™ Maximum Recovery Diluent (Dehydrated).

Maximum Recovery Diluent combines the protective effect of peptone in the diluting solution1 with the osmotic support of physiological saline. The low peptone concentration in the diluent reduces organism multiplication in the sample for at least 45 minutes during the dilution stage.

Maximum Recovery Diluent, Oxoid Composition

Maximum Recovery Diluent, Oxoid Preparation:
Dissolve 9.5g in 1 litre of distilled water. Dispense into the final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

• Oxoid AFPA Medium Base is a selective identification medium for the detection of Aspergillus flavus and Aspergillus parasiticus.
• Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.

AFPA Medium Base, Oxoid Composition

AFPA Medium Base, Oxoid Preparation:

Suspend 22.75g in 500ml (45.5g/l) of distilled water and heat to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the AFPA Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

• Oxoid Dichloran-Glycerol (DG18) Agar Base is a selective low water activity (aw) medium for xerophilic molds from dried and semi-dried foods.
• Also available Chloramphenicol Selective Supplement, Part No. SR0078E and Chloramphenicol Selective Supplement, Part No. SR0078H.

Dichloran-Glycerol (DG18) Agar Base, Oxoid Composition

Dichloran-Glycerol (DG18) Agar, Oxoid Preparation:
Suspend 15.75g in 500ml (31.5g/l) of distilled water and heat to dissolve completely. Add 110g per 500ml (220g/l) of Glycerol (Analytical Reagent grade). Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the DG18 Agar Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

• Oxoid DRBC Agar Base (Dichloran Rose-Bengal Chloramphenicol Agar Base) is a selective medium for yeast and molds associated with food spoilage.
• Also available Chloramphenicol Selective Supplement, Part No. SR0078E and Chloramphenicol Selective Supplement, Part No. SR0078H

DRBC Agar Base, Oxoid Composition

DRBC Agar, Oxoid Preparation:
Suspend 15.75g in 500ml (31.5g/l) of distilled water and heat to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the DRBC Agar Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

• Oxoid K-F Streptococcus Agar is a selective medium for the isolation and enumeration of Group D Streptococci.
• Also available TTC Solution, Part No. SR0148A.

K-F Streptococcus Agar Base, Oxoid Composition

K-F Streptococcus Agar, Oxoid Preparation:
Suspend 38.2g in 500ml of distilled water. Bring to the boil with frequent agitation. Boil for 5 minutes. Cool to 50°C and add aseptically 1 vial (5ml) of 1% 2,3,5-Triphenyltetrazolium chloride (SR0229K). Pour into sterile Petri dishes when using the membrane filtration method or hold at 45°C when using the pour-plate method.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
Observe the HAZARD precautions regarding sodium azide when disposing of the medium. The pH of the medium should not fall below 7.0 as it may become inhibitory towards enterococci. KF Streptococcus Agar is not specific for the presumptive identification of Group D streptococci. Further tests must be made to confirm the identity of the organisms isolated.

Enrich and improve the isolation of salmonellae from feces and food samples with Thermo Scientific™ Oxoid™ Selenite Cystine Broth Base. This modified medium is based upon the formulation described by Leifson with added cystine.

Selenite Cystine Broth Base, Oxoid Composition

Selenite Cystine Broth, Oxoid Preparation:
Dissolve 4g of sodium biselenite LP0121 in 1 litre of distilled water and then add 19g of Selenite Cystine Broth Base. Warm to dissolve and dispense into containers. Sterilise by placing in free flowing steam for 15 minutes. DO NOT AUTOCLAVE.

Robertson reported miscarriages and possible tetragenic effects on pregnant laboratory workers which may have been caused by the ingestion of sodium biselenite. To minimise any possible risk of teratogenicity to laboratory workers the sodium biselenite is not included in the dry powder but should be prepared separately as a solution to which the Selenite Cystine Broth Base is added.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Precautions
Observe the precautionary comments made about sodium biselenite in Selenite Broth Base CM0395.
Discard the prepared medium if large amounts of reduced selenite can be seen as a red precipitate in the bottom of the bottle.
Do not incubate longer than 24 hours because the inhibitory effect of selenite is reduced after 6-12 hours incubation.
Take subcultures of broth from the upper third of the broth column which should be at least 5 cm in depth.

• Oxoid Campylobacter Agar Base is used for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian and environmental specimens.
• Also available Campylobacter Selective Supplement (Blaser-Wang), Part No. SR0098E NMKL; Bolton Broth Selective Supplement, Part No. SR0183E; Modified Bolton Broth Selective Supplement, Part No. SR0208E; Modified Preston Campylobacter Selective Supplement, Part No. SR0204E; and Campylobacter Selective Supplement Preston, Part No. SR0117E.

Campylobacter Agar Base, Oxoid Composition

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Isolate and enumerate microorganisms in milk and dairy products with Thermo Scientific™ Oxoid™ Milk Plate Count Agar (Dehydrated). Milk Plate Count Agar is equivalent to the medium recommended by British Standard Institution and International Organization for Standardization.

Milk Plate Count Agar (Dehydrated), Oxoid Composition

Milk Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 19.5g in 1 litre of distilled water. Bring to the boil with frequent agitation, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Tetrathionate Broth is used for the selective enrichment of Salmonella spp.

Tetrathionate Broth, Oxoid Composition

Tetrathionate Broth, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water and bring to the boil. Cool to below 45°C and add 20ml of iodine-iodide solution immediately before use. Mix continuously whilst dispensing 10ml volumes into sterile tubes. Use the complete medium (with added iodine) on the day of preparation.

The base may be prepared beforehand and kept for several weeks at 4°C. Iodine-iodide solution can then be added at the time of use to the quantity of medium needed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the base broth at 2-8°C. Use the medium immediately after adding the iodine solution.

Selectively enrich Salmonella spp. from food and environmental specimens with Thermo Scientific™ Oxoid™ Rappaport-Vassiliadis Enrichment Broth (Dehydrated). Rappaport-Vassiliadis Enrichment Broth selects for Salmonella species by exploiting their characteristics when compared with other Enterobacteriaceae, such as the ability to survive at relatively high osmotic pressure, multiply at relatively low pH values, be relatively more resistant to malachite green, and have relatively less demanding nutritional requirements.

Rappaport-Vassiliadis Enrichment Broth (Dehydrated), Oxoid Composition

THIS MEDIUM IS VERY HYGROSCOPIC AND MUST BE PROTECTED FROM MOISTURE.
The quantities given for the formula as classically described made 1110ml of medium. They have been published this way in the Oxoid literature to coincide with the scientific literature.

The directions for reconstituting Oxoid Rappaport-Vassiliadis (RV) Enrichment Broth follow usual Oxoid practice and specify the weight needed for 1 litre of medium.

Rappaport-Vassiliadis Enrichment Broth (Dehydrated), Oxoid Preparation:
Add 30g (the equivalent weight of dehydrated medium per litre) to 1 litre of distilled water. Heat gently until dissolved completely. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
RV Broth should not be used if Salmonella typhi is suspected.
Note the difference in weight between the classical formula on the label and the reduced weight per litre, using anhydrous magnesium chloride.

Isolate and enumerate spoilage organisms of citrus products with Thermo Scientific™ Oxoid™ Orange Serum Agar (Dehydrated). Orange Serum Agar is developed specifically for the isolation and enumeration of microorganisms that are capable of surviving citrus products. The low pH of these products limits the growth of microorganisms to those capable of tolerating the acidic environment.

Orange Serum Agar, Oxoid Composition

Orange Serum Agar, Oxoid Preparation:
Suspend 37g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Isolate Legionella from environmental samples and clinical specimens using Thermo Scientific™ Oxoid™ Legionella CYE Agar Base when used with Legionella Growth Supplement SR01110. Legionella CYE Agar Base is a non-selective medium that exhibits optimal recovery of legionellaceae in short incubation periods from both environmental samples and clinical specimens.

Legionella CYE Agar Base, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

• Oxoid Yersinia Selective Agar Base (Schiemann’s CIN Agar) is used for the isolation and enumeration of Yersinia enterocolitica from clinical specimens and foods.
• Also available Yersinia Selective Supplement, Part No. SR0109E. Industrial Reference: FDA.

Yersinia Selective Agar Base, Oxoid Composition

Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.

Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.

**This product is not available at the moment. Please contact us for more information.

Oxoid Universal Beer Agar is used for the isolation of beer spoilage microorganisms.

Universal Beer Agar, Oxoid Composition

Universal Beer Agar, Oxoid Preparation:
Suspend 62g in 750ml of distilled water and bring to the boil to dissolve completely. Add 250ml beer, without degassing, to the hot medium and mix gently. Distribute into final containers and sterilise by autoclaving at 121°C for 10 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
When handling cycloheximide observe the precautions to be taken under HAZARDS.