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Isolate and enumerate coliforms in food, animal feed and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), while adhering to ISO 4832:2006 and ISO/TS 11133-2:2003. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBL Agar (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBL Agar (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBL Agar (ISO) plates can be stored for up to 2 weeks when made from CM0968 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBL Agar (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBL Agar (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

• Oxoid Arcobacter Broth Base is used for the selective growth of Arcobacter spp.
• Also available CAT Supplement, Part No. SR0174E and CCDA Selective Supplement, Part No. SR0155E and SR0155H.

Arcobacter Broth Base, Oxoid Composition

Arcobacter Broth Base, Oxoid Preparation:

Dissolve 12g of Arcobacter Broth in 500ml of distilled water. Sterilise at 121°C for 15 minutes and allow to cool to below 50°C

For use with CAT supplement (SR0174)

Add one vial per 500ml medium of CAT Selective Supplement reconstituted as directed. Dispense into sterile containers.

For use with CCDA supplement (SR0155)

Add one vial per 500ml medium of CCDA Selective Supplement reconstituted as directed. Dispense into sterile containers.

Inoculated plates should be incubated aerobically, at 30°C, for 24 hours.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

• The Oxoid Baird-Parker RPF Agar Base is a selective medium for the isolation and enumeration of coagulase positive staphylococci from food.
• For use with RPF supplement, Part No. SR0122A
• Do not use Egg Yolk Tellurite Emulsion.

Baird-Parker Agar Base (RPF), Oxoid Composition

Baird-Parker Agar Base (RPF), Oxoid Preparation:
Suspend 6.3g of Baird-Parker Agar Base (RPF) in 90ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121ºC for 15 minutes. Cool to 48ºC and add 1 vial of SR0122A, reconstituted as directed. Mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and RPF Supplement below 0°C. Use before the expiry date on the label.
Prepared plates of medium are best used freshly prepared.

Precautions
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

Cultivate anaerobic microorganisms, particularly Bacteroides spp. and other fastidious anaerobes with Thermo Scientific™ Oxoid™ Anaerobe Basal Agar (Dehydrated). Anaerobe Basal Broth is formulated from a range of nutrients which have been selected to optimize the recovery and growth of the majority of anaerobic organisms of clinical importance.

Anaerobe Basal Broth, Oxoid Composition

Anaerobe Basal Broth, Oxoid Preparation:
Suspend 35.4g of Anaerobe Basal Broth in 1 litre of distilled water. Bring to the boil to dissolve. Distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Anaerobe Basal Broth should be stored tightly capped in the original container at 10-30°C. When stored as directed, the medium will remain stable until the expiry date printed on the label.

Brilliance E. coli/Coliform Agar is a chromogenic medium for differentiation of Escherichia coli and other coliforms from food and environmental samples.

Brilliance E. coli/Coliform Agar, Oxoid Composition

Brilliance E. coli/Coliform Agar, Oxoid Preparation:
Suspend 55.8g of Brilliance E.coli/coliform agar in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour and into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C, and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Refer to the material safety data sheet before handling this product.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give purple colonies on this medium.

Detect and enumerate Escherichia coli in food and animal feed samples with Thermo Scientific™ Oxoid™ Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated). The medium meets the formulation and performance criterion laid down in ISO 16649 and ISO 11133:2014.

TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity, an enzyme shown to be highly specific for Escherichia coli.

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Composition

Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Preparation:
Suspend 36.6g of TBX Medium in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour the medium into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

• Oxoid Campylobacter Karmaili Agar Base is a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42°C.
• Also available Campylobacter Selective Supplement (Karmaili), Part No. SR0167E and Modified Karma-i-li Selective Supplement, Part No. SR0205E.

Campylobacter Karmaili Agar Base, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Add 21.5g of Campylobacter Agar Base (Karmali) to 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of Campylobacter Selective Supplement (Karmali) SR0167 or Modified Karmali Selective Supplement SR0205, reconstituted as directed.. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Selectively enumerate Bacillus cereus in food samples with Thermo Scientific™ Oxoid™ MYP Agar (Dehydrated). The medium relies upon mannitol fermentation and phospholipase C production to differentiate Bacillus cereus from other members of the species.

Mannitol-negative Bacillus cereus form pink colored colonies due to the presence of Phenol Red Indicator. Egg yolk emulsion helps in the differentiation of phospholipase C producing colonies, which are surrounded by a zone of the white precipitate. Polymixin B Sulphate acts as the inhibitor to restrict the growth of Gram-negative bacteria.

MYP Agar, Oxoid Composition

MYP Agar, Oxoid Preparation:
Suspend 21.5g in 450ml distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes.
Cool to approximately 49°C and aseptically add 50ml Egg Yolk Emulsion SR0047 and 1 vial of Polymyxin B Supplement SR0099, reconstituted as directed. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thermo Scientific™ Oxoid Yeast and Mold Agar is used for the isolation and maintenance of yeast and molds.

Yeast and Mold Agar, Oxoid Composition

Yeast and Mold Agar, Oxoid Preparation:
Suspend 41.0g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. The medium may be rendered selective after sterilisation by acidifying to pH 4.0 with 12-15ml of Lactic Acid SR0021 after cooling to 50°C. Do not reheat after making this addition. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
For in vitro diagnostic use.
Do not use beyond the stated expiry date or if the product is caked, discoloured or shows any sign of deterioration.

• Oxoid Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium) is used for the detection of motile Salmonella spp. from food and environmental samples.
• Also available MSRV Selective Supplement, Part No. SR0161E and Novobiocin Supplement, Part No. SR0181E.

Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium), Oxoid Composition

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Precautions
The basal medium is very hygroscopic. When handling the powder a face mask and gloves must be worn, refer to the material safety data sheet.

Enumerate heterotrophic bacteria from drinking water samples with Thermo Scientific™ Oxoid™ R2A Agar (Dehydrated). This nutritionally reduced medium was specially designed to enhance the recovery of stressed and chlorine damaged bacteria from treated water samples. Stressed or injured organisms during water treatment are unable to grow on high nutrient media since faster-growing organisms outgrow the former. Therefore the use of a low nutrient medium like R2A Agar incubated for longer incubation periods allows these stressed organisms to grow well.

R2A Agar, Oxoid Composition

R2A Agar, Oxoid Preparation:
Suspend 18.1g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
R2A Agar should be stored tightly capped in the original container at 10-30°C. When stored as directed the medium will remain stable until the expiry date printed on the label.

The prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Haemophilus Test Medium Base (HTM) is used for the susceptibility testing of Haemophilus influenzae.
• Also available HTM Supplement, Part No. SR0158E. Store at -20° to 0°C.

Haemophilus Test Medium Base (HTM), Oxoid Composition

Haemophilus Test Medium (HTM), Oxoid Preparation:
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
When stored as directed the supplement is stable until the expiry date stated on the label.

Oxoid Buffered Listeria Enrichment is selective enrichment medium for the detection of Listeria monocytogenes.

Buffered Listeria Enrichment Broth, Oxoid Composition

Buffered Listeria Enrichment, Oxoid Preparation:
Add 23.5g to 500ml of distilled water and mix well to dissolve. Add the contents of 1 vial of Listeria Selective Enrichment Supplement SR0141 or the alternative Modified Listeria Selective Supplement SR0213 reconstitued as directed. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and aseptically distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Buffered Listeria Enrichment Broth should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored as directed the medium will remain stable until the expiry date printed on the label.

Isolate Listeria spp. from food and environmental specimens using Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated). The medium is intended for the isolation of Listeria spp. when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Fraser Broth enrichment cultures should be sub-cultured to plating medium.

Fraser Broth Base, Oxoid Composition

Fraser Broth, Oxoid Preparation:
With Fraser Supplement:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

With Half Fraser Supplement:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers. Alternatively, to make 2.25 litres, use 129.0g of CM0895 and 1 vial of SR0166G.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Isolate and presumptively identify enterococci / Group D streptococci with Thermo Scientific™ Oxoid™ Bile Aesculin Agar (Dehydrated). Bile Aesculin Agar is designed to differentiate between Group D streptococci and non-Group D streptococci.

Group D streptococci hydrolyse aesculin to form aesculetin and dextrose. Aesculin hydrolysis is indicative of a positive result. Bile salts in the medium inhibit Gram-positive bacteria other than enterococci/ Group D streptococci.

Bile Aesculin Agar, Oxoid Composition

Bile Aesculin Agar, Oxoid Preparation:
Suspend 44.5g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

• Oxoid STAA Agar Base is used for the isolation of Brochothrix thermosphactafrom food samples.
• Also available STAA Selective Supplement, Part No. SR0151E and STA Selective Supplement, Part No. SR0162E.

STAA Agar Base, Oxoid Composition

STAA Agar, Oxoid Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add:

a) for STAA Medium the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

b) for STA Medium the contents of 1 vial of STA Selective Supplement SR0162 reconstituted as directed.
Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid PALCAM Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
• Also available PALCAM Selective Supplement, Part No. SR0150E or SR0150B.

PALCAM Agar Base, Oxoid Composition

PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.

The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.

Precautions
Acriflavine hydrochloride is activated by light which may cause it to become inhibitory to Listeria growth.

Oxoid Azide Dextrose Broth (Rothe Broth) is used for the detection of enterococciin water, sewage, and food.

Azide Dextrose Broth (Rothe Broth), Oxoid Composition

Azide Dextrose Broth (Rothe Broth), Oxoid Preparation:
Add 35.6g to one litre of distilled water for single strength broth or 71.2g for double strength broth. Heat gently to dissolve. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
This product contains less than 1% azide and has low toxicity. However, when handling the powder, wear gloves, mask and eye protection. When washing azide products down sinks, use sufficient water to prevent accumulation of azide in the plumbing.

Selectively enrich Salmonella spp. from food and environmental specimens with Thermo Scientific™ Oxoid™ Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated). Rappaport- Vassiliadis Soya Peptone Broth selects for Salmonella species by exploiting their characteristics when compared with other Enterobacteriaceae, such as the ability to survive at relatively high osmotic pressure, multiply at relatively low pH values, be relatively more resistant to malachite green, and have relatively less demanding nutritional requirements.

Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated), Oxoid Composition

Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated), Oxoid Preparation:
Suspend 26.75g in 1 litre of distilled water and heat gently to dissolve. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
RVS Broth should not be used if Salmonella Typhi is suspected.
In order to achieve optimum recovery it is recommended that the enrichment broth is incubated at 42 ± 1°C.

• Oxoid Listeria Enrichment Broth Base (UVM Formulation) is a two-step selective enrichment (USDA-FSIS) method.
• Also available Listeria Primary Enrichment Supplement-UVM I, Part No. SR0142E or SR0142B and Listeria Secondary Selective Enrichment Supplement-UVM II, Part No. SR0143E.
  Listeria Enrichment Broth Base (UVM formulation) is to be used with the appropriate supplement for UVMI broth or UVMII broth.

Listeria Enrichment Broth Base (UVM formulation), Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.

To Prepare Listeria Primary Selective Enrichment Medium (UVM I):
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

To Prepare Listeria Secondary Selective Enrichment Medium (UVM II):
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

• Oxoid Listeria Enrichment Broth is used for the enrichment of Listeria species.
• Also available Listeria Selective Supplement, Part No. SR0140E or SR0141E and Modified Listeria Selective Enrichment Supplement, Part No. SR0206E or SR0206B.

Listeria Enrichment Broth Base, Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 18g in 500ml of distilled water. Add the contents of one vial of Listeria Selective Enrichment Supplement (SR0141), reconstituted with 2ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140. Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against Listeria.
Supplement SR0141 used in this medium contains a toxic concentration of cycloheximide. Note the precautions to be taken under HAZARDS.

Oxoid Salmonella Rapid Test Elective Medium (SRTEM) is a selective enrichment broth medium specifically formulated for use with the Oxoid Salmonella Rapid Test Kit, Part No. FT0201A.

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Composition

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Preparation:
Add 61g to 1 litre of distilled water (double strength medium). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 18-25°C and use as described in the Oxoid Salmonella Rapid Test (Code FT0201) instruction leaflet.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

• Oxoid Listeria Selective Oxford Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
• Also available Listeria Selective Supplement, Part No. SR0140E and Modified Listeria Selective Supplement, Part No. SR0206E or SR0206B.

Listeria Selective Oxford Agar Base, Oxoid Composition

Listeria Selective Oxford Agar, Oxoid Preparation:
Suspend 27.75 of the Listeria Selective Agar Base (Oxford Formulation) in 500ml of distilled water. Bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Listeria Selective Supplement (Oxford Formulation) or Modified Listeria Selective Supplement (Oxford) SR0206, SR0140 reconstituted with 5ml of 70% ethanol. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Cultivate and isolate fastidious microorganisms from clinical samples through improved hemolytic reactions with sheep blood with Thermo Fisher™ Oxoid™ Sheep Blood Agar Base (Dehydrated) when used with sheep blood. Sheep Blood Agar Base was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of organisms without interfering with their hemolytic reactions when used with sheep blood.

Sheep Blood Agar Base (Dehydrated), Oxoid Composition

Sheep Blood Agar Base (Dehydrated), Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 7% sterile sheep blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.