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Simmons Citrate Agar (Dehydrated), Oxoid Composition

Simmons Citrate Agar (Dehydrated), Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is important not to carry over any nutrients into the citrate medium because this will result in false positive tests. Dilute the inoculum in saline before inoculating the citrate medium to avoid a carry-over of other carbon sources.

Oxoid Skim Milk Powder is a thermophile-free, spray dried powder for inclusion in bacteriological culture media.

Detect and enumerate enterococci from water and food samples with Thermo Scientific™ Slanetz and Bartley Medium (Dehydrated). Slanetz and Bartley originally devised this medium to detect and enumerate enterococci by the technique of membrane filtration, but it has also proved useful as a direct plating medium. The medium is very selective for enterococci and, when it is incubated at elevated temperatures (44-45°C), all red or maroon colonies may be accepted as presumptive enterococci.

Slanetz and Bartley Medium (Dehydrated), Oxoid Composition

Slanetz and Bartley Medium Preparation:
Suspend 42g in 1 litre of distilled water and bring to the boil to dissolve the agar completely.

EXCESSIVE HEATING MUST BE AVOIDED. Dispense into Petri dishes and allow to solidify. It should not be remelted. The medium may be used with membrane filters or by spreading dilutions of the sample over the surface of the agar with a glass rod.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Precautions
Count all red, maroon or pink colonies as presumptive enterococci. Not all species reduce TTC therefore pale colonies should not be ignored.
Although incubation at 35°C yields a higher count, it allows the growth of organisms which do not conform to the definition of enterococci. Incubation at 44-45°C has a selective effect and produces fewer false-positives. However, the preliminary incubation at 35°C encourages the recovery of stressed organisms.
Although the selective properties of this medium are very good it is advisable to regard the colony count as a presumptive or unconfirmed count. Further identification may be required depending on the scope of the examination.

Use Thermo Scientific™ Oxoid™ Sodium Biselenite in preparation of Selenite Broth Base (CM0395), Mannitol Selenite Broth Base (CM0399) or Selenite Cystine Broth Base (CM0699).  The complete medium is recommended for selective enrichment of Salmonella species from various samples.

• Dissolve 4 grams in 1 liter of distilled water and use this solution to reconstitute the base medium or tablets.
• Toxic by inhalation and if swallowed. Danger of cumulative effects.
• Not all products are available for sale in all territories. Please inquire.
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Oxoid Sodium Chloride Bacteriological is a dehydrated salt suitable for inclusion in bacteriological culture media.

Carry out microbial limit testing by harmonized methodology of pharmaceutical products in accordance with USP/EP/BP/JP with Thermo Scientific™ Oxoid™ Buffered Sodium Chloride Peptone Solution (Dehydrated). The combination of phosphate buffer, sodium chloride and peptone increases the viability of sensitive microorganisms by providing an isotonic environment. This is of particular importance when trying to recover cells that may be stressed or sensitive to osmotic pressure.

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Composition

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Preparation:
Suspend 14.63g of Sodium Chloride Peptone Broth (Buffered) in 1 litre of water (purified as required). If required, add 1-10ml Tween® 20 or 80 per litre of medium. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared media at room temperature.