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LB agar (MILLER) for Microbiology (Merck)

Brand:

Merck

Description

LB Agar Miller from Merck is a commonly used medium in microbiology laboratories and is suitable for routine bacterial culture and transformation experiments. It is a formulation of Luria-Bertani (LB) agar developed by Miller, which includes a mixture of peptides, amino acids, and vitamins to support the growth of a wide range of bacteria.

LB broth (MILLER) for Microbiology (Merck)

Brand:

Merck

Description

LB-Broth is based on the formulation of MILLER (1972) supporting the growth of E. coli. Casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins.

Typical Composition of LB-Broth (Miller, Merck)

Typical Formula *
gm/litre
Yeast extract

5.0

Peptone from casein

10.0

Sodium chloride

10.0

Preparation of LB Broth (Miller)

Suspend 25 g LB-Broth in 1 litre of demineralized water and autoclave for 15 min at 121 °C.  at 25 °C (pH: 7.0 ± 0.2). The prepared broth is clear and yellowish-brown.

Experimental Procedure

According to appropriate use or purpose. Incubation: 24 h at 35-37 °C aerobically.

Product Specification and MSDS for LB Broth, Miller (Merck).

LCAT Selective Supplement 1 x 10 Vials

Brand:

Thermo ScientificTM OxoidTM

Oxoid LCAT Selective Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.

  • Add to GC Agar Base, Part No. CM0367B
  • Each vial supplements 500mL of medium

LCAT Selective Supplement, Oxoid Composition

Vial contents (each vial is sufficient for 500ml of medium)
Lincomycin

0.5 mg

Colistin sulphate

3.0 mg

Amphotericin B

0.5 mg

Trimethoprim

3.25 mg

 

LCAT Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 490ml of sterile GC Agar Base CM0367, cooled to approximately 50°C, containing 50 ml Laked Horse Blood SR0048 and the rehydrated contents of one vial of Yeast Autolysate Supplement SR0105. Mix gently and pour into sterile Petri dishes.

Legionella (GVPC) Selective Supplement 1 x 10 Vials

Brand:

Thermo ScientificTM OxoidTM

Oxoid Legionella GVPC Selective Supplement is used for the isolation of Legionella spp. from environmental samples.

  • Add to Legionella CYE Agar Base, Part No. CM0655B
  • Each vial supplements 500mL of medium

Legionella GVPC Selective Supplement, Oxoid Composition

Vial contents (each vial is sufficient to supplement 500 ml medium)
Glycine (Ammonia free)

1.5 g

Vancomycin hydrochloride

0.5 mg

Polymyxin B sulphate

40000 IU

Cycloheximide

40.0 mg

 

Legionella GVPC Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Legionella CYE Agar Base CM0655 to which one vial of Legionella BCYEa Legionella Growth Supplement SR0110C has been added cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Legionella BCYE Growth Supplement

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
  • Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

Vial contents

SR0110C
(1 vial per 500ml medium)

Supplement
per litre
Buffer/Potassium hydroxide
5.0g
10g
Ferric pyrophosphate
0.125g
0.25g
L-cysteine HCl
0.2g
0.4g
a -Ketoglutarate
0.5g
1.0g

 

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Legionella BCYE Growth Supplement 1 x 10 Vials

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
  • Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

Vial contents

SR0110A
(1 vial per 100ml medium)

Supplement
per litre
Buffer/Potassium hydroxide
1.0g
10g
Ferric pyrophosphate
0.025g
0.25g
L-cysteine HCl
0.04g
0.4g
a -Ketoglutarate
0.1g
1.0g

 

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Legionella BCYE Supplement w/o L-Cysteine 1 x 10 Vials

Brand:

Thermo ScientificTM OxoidTM

Oxoid Legionella BCYE Supplement w/o L-cysteine is used for the isolation of Legionella spp.

  • Add to Legionella CYE Agar Base, Part No. CM0655B
  • Each vial supplements 100mL of medium

Legionella BCYE Growth Supplement without L-cysteine, Oxoid Composition

Vial contents (each vial is sufficient for 100ml of medium)
ACES Buffer/Potassium hydroxide

1.0g

Ferric pyrophosphate

0.025g

a -ketoglutarate

0.1g

 

Legionella BCYE Growth Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90ml of sterile Legionella CYE Agar Base (CM0655) cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Legionella BMPA-α Selective Supplement 1X10 Vials

Brand:

Thermo ScientificTM OxoidTM

Thermo Scientific™ Oxoid Legionella BMPA-α Selective Supplement is a selective supplement for the isolation of legionellae when used with Legionella BCYE Growth Supplement (SR0010) and Legionella CYE Agar Base (CM0655).

Legionella BMPA-α Selective Supplement, Oxoid Composition

Vial contents

 SR0111E
(1 vial per 100ml medium)

per litre

Cefamandole

400µg

4.0mg

Polymyxin

8,000IU

80,000IU

Anisomycin

8.0mg

80.0mg

 

Legionella BMPA-α Selective Medium Preparation:
Reconstitute one vial SR0111E per 100ml medium as directed. Aseptically add the vial contents to BCYE-a Medium prepared from Legionella CYE Agar Base (CM0655) and Legionella BCYE Supplement (SR0110) and cooled to 50°C. Mix well and pour into sterile Petri dishes.

Legionella CYE Agar Base 500g

Brand:

Thermo ScientificTM OxoidTM

Isolate Legionella from environmental samples and clinical specimens using Thermo Scientific™ Oxoid™ Legionella CYE Agar Base when used with Legionella Growth Supplement SR01110. Legionella CYE Agar Base is a non-selective medium that exhibits optimal recovery of legionellaceae in short incubation periods from both environmental samples and clinical specimens.

Legionella CYE Agar Base, Oxoid Composition

Typical Formula*

gm/litre

Activated charcoal

2.0

Yeast extract

10.0

Agar

13.0

 

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Leishman’s Eosin Methylene Blue Solution Modified (Merck)

Brand:

Merck

Description

The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright’s eosin methylene blue solution and May-Grünwald’s eosin methylene blue solution, it is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). 500 mL of the IVD & CE-registered Leishman’s eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells. It is registered as IVD and CE product.

Application

For microscopy. For staining of blood and bone marrow smears and clinical-cytological specimens

Product Specification and MSDS for Leishman’s eosin methylene blue solution modified (Merck).

Leupeptin, 100 mg

Brand:

Merck

Description

Leupeptin is an organic compound produced by actinomycetes, serine and cysteine proteases reversibly. Inhibits trypsin-like proteases and some cysteine proteases including endoproteinase Lys-C, Kallikrein, papain, thrombin, cathepsin B and trypsin.
MW:426.56 s 49.04 . 18.02 {C20H36N6O4 . ½H2SO4 . H2O}
Ac-Leu-Leu-Argininal (hemi-sulfated form). Thus material has three tautomeric isomers that can be visualized by chromatography.
Appearance: White amorphous powder
Working range 10-100μM. Stable for several hours in water. Stock solutions of 10mM in water are stable for 1 week at 4ºC and 6 months at -20ºC.
5mg/1.054 mL = 10mM. Typically dilute 1:100 for a 100μM working solution.
Source: Organic isolated from actinomycetes.

Maintain dry at -20ºC for up to 18 months after date of receipt. Store reconstituted product in aliquots at -20ºC for up to 6 months. Avoid repeated thaw freeze cycles.

Lev1 Levofloxacin

Brand:

Thermo ScientificTM OxoidTM

** This item is not available at the moment. Please contact us for more information.
Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Levofloxacin Antimicrobial Susceptibility Disks.

Lev5 Levofloxacin

Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Levofloxacin Antimicrobial Susceptibility Disks.

Levo ME Pipette Filler

Brand:

Biologix

This product is not available at the moment. Please contact us for more information.

The Levo ME Pipette Filler has improved ergonomics and streamlined function for a more convenient pipetting experience.

Features
• Ergonomic shape and intuitive operation
• Pressure-sensitive buttons: increasing pressure increases aspiration/dispensing speed
• Autoclavable hydrophobic filter prevents over-aspiration
• Rechargeable, long-lasting battery
• LED light indicates aspiration/dispensing, charging, and low battery
• Compatible with almost all standard glass or plastic pipettes

Levo ME Pipette Filler Brochure

Levo Plus Pipette Filler

Brand:

Biologix

This product is not available at the moment. Please contact us for more information.

The Levo Plus pipette filler efficiently aspirates and dispenses liquids, its powerful motor filling a 25 mL pipette in less than 5 seconds. It is compatible with most standard glass and plastic pipettes with volumes ranging from 0.1 – 100 mL.

Features
• Lightweight, ergonomic design
• Convenient one-handed operation
• Eight aspiration and dispensing speeds
• LCD display shows speed settings and remaining battery life
• Efficient lithium-ion battery can last for up to 8 hours of intermittent use
• Replaceable hydrophobic 0.45 μm filter
• Integral, autoclavable silicone adapter suits use with 0.1 – 100 mL pipettes
• Can be used while charging

Levo Plus Pipette Filler Brochure

Levofloxacin 32 M.I.C.E. (10 Pack)

Brand:

Thermo ScientificTM OxoidTM

**This product is not available at the moment.

Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Levofloxacin M.I.C.Evaluator Strips (M.I.C.E.™).

Levofloxacin 32 M.I.C.E. (50 Pack)

Brand:

Thermo ScientificTM OxoidTM

**This product is not available at the moment.

Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Levofloxacin M.I.C.Evaluator Strips (M.I.C.E.™).

Linezolid 256 M.I.C.E. (10 pack)

Brand:

Thermo ScientificTM OxoidTM

**This product is not available at the moment.

Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Linezolid M.I.C.Evaluator Strips (M.I.C.E.™).

Linezolid 256 M.I.C.E. (50 Pack)

Brand:

Thermo ScientificTM OxoidTM

**This product is not available at the moment.

Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Linezolid M.I.C.Evaluator Strips (M.I.C.E.™).

Listeria Enrichment Broth Base 500g

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Listeria Enrichment Broth is used for the enrichment of Listeria species.
  • Also available Listeria Selective Supplement, Part No. SR0140E or SR0141E and Modified Listeria Selective Enrichment Supplement, Part No. SR0206E or SR0206B.

Listeria Enrichment Broth Base, Oxoid Composition

Typical Formula*

gm/litre

Tryptone soya broth

30.0

Yeast extract

6.0

pH 7.3 ± 0.2 @ 25°C

 

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 18g in 500ml of distilled water. Add the contents of one vial of Listeria Selective Enrichment Supplement (SR0141), reconstituted with 2ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140. Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against Listeria.
Supplement SR0141 used in this medium contains a toxic concentration of cycloheximide. Note the precautions to be taken under HAZARDS.

Listeria Enrichment Broth Base UVM 500g

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Listeria Enrichment Broth Base (UVM Formulation) is a two-step selective enrichment (USDA-FSIS) method.
  • Also available Listeria Primary Enrichment Supplement-UVM I, Part No. SR0142E or SR0142B and Listeria Secondary Selective Enrichment Supplement-UVM II, Part No. SR0143E.
    Listeria Enrichment Broth Base (UVM formulation) is to be used with the appropriate supplement for UVMI broth or UVMII broth.

Listeria Enrichment Broth Base (UVM formulation), Oxoid Composition

Typical Formula*

gm/litre

Proteose peptone

5.0

Tryptone

5.0

`Lab-Lemco’ powder

5.0

Yeast extract

5.0

Sodium chloride

20.0

Disodium hydrogen phosphate

12.0

Potassium dihydrogen phosphate

1.35

Aesculin

1.0

pH 7.4 ± 0.2 @ 25°C

 

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.

To Prepare Listeria Primary Selective Enrichment Medium (UVM I):
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

To Prepare Listeria Secondary Selective Enrichment Medium (UVM II):
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

Listeria Primary Enrichment Supplement (UVMI) 1X10Vials

Brand:

Thermo ScientificTM OxoidTM

Thermo Scientific™ Oxoid Listeria Primary Enrichment Supplement (UVMI) is a primary selective enrichment supplement for the isolation of listeria when used with Listeria Enrichment Broth Base (UVM Formulation) CM0863.

Oxoid Listeria Primary Enrichment Supplement (UVMI), Oxoid Composition

Vial contents (each vial is sufficient for 500ml of medium)
per vial
per litre
Nalidixic acid

10.0mg

20.0mg
Acriflavine hydrochloride

6.0mg

12.0mg

 

Listeria Primary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

Listeria Secondary Enrichment Supplement (UVMII) 1x10v

Brand:

Thermo ScientificTM OxoidTM

Oxoid Listeria Secondary Selective Enrichment Supplement, UVMII is used in a two-step selective enrichment (USDS-FSIS) method for the enrichment of Listeria spp.

  • Add to Listeria Enrichment Broth Base (UVM formulation), Part No. CM0863B
  • Each vial supplements 500mL of medium

Listeria Secondary Selective Enrichment Supplement (UVMII), Oxoid Composition

Vial contents (each vial is sufficient for 500ml of medium)
per vial
per litre
Nalidixic acid

10.0mg

20.0mg
Acriflavine hydrochloride

12.5mg

25.0mg

 

Listeria Secondary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

Listeria Selective Agar Base 500g

Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Listeria Selective Oxford Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
  • Also available Listeria Selective Supplement, Part No. SR0140E and Modified Listeria Selective Supplement, Part No. SR0206E or SR0206B.

Listeria Selective Oxford Agar Base, Oxoid Composition

Typical Formula*

gm/litre

Columbia Blood Agar Base

39.0

 Aesculin

1.0

 Ferric ammonium citrate

0.5

 Lithium chloride

15.0

 pH 7.0 ± 0.2 @ 25°C

 

Listeria Selective Oxford Agar, Oxoid Preparation:
Suspend 27.75 of the Listeria Selective Agar Base (Oxford Formulation) in 500ml of distilled water. Bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Listeria Selective Supplement (Oxford Formulation) or Modified Listeria Selective Supplement (Oxford) SR0206, SR0140 reconstituted with 5ml of 70% ethanol. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.