RNase and DNAase Away
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
RNase and DNAase AwayTM: DB0339 efficiently remove surface-contaminant from glasswares and plasticwares without having a residual effect on subsequent DNA & RNA samples . The product provides more effective at degradading DNA than autoclave.
RNase Erase
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MP Biomedicals
RNase ERASE® reagent is a precisely formulated, ready-for-use, ribonuclease decontaminant. RNase ERASE® Reagent will effectively remove RNA and RNase contamination from benchtop, plastic, stainless steel and glass lab surface.
RNaseZAP™ (Sigma-Aldrich)
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Sigma-Aldrich
Description
Cleaning agent for removing RNase
Application
RNA purification: suitable
RNaseZAP™ has been used as a cleaning agent for removing RNase from glassware, apparatus, countertops and pipettors.
A cleaning agent for removing RNase from glassware, plastic surfaces, countertops, and pipettors. It is also effective at eliminating RNase contamination from microcentrifuge tubes without inhibiting subsequent enzymatic reactions.
Rose Bengal Chloramphenicol Agar 500g
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Thermo ScientificTM OxoidTM
- Oxoid Rose-Bengal Chloramphenicol Agar Base is a medium for the selective enumeration of yeast and molds from foods.
- Also available Chloramphenicol Selective Supplement, Part No. SR0078E.
Rose-Bengal Chloramphenicol Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre |
| Mycological peptone |
5.0 |
| Glucose |
10.0 |
| Dipotassium phosphate |
1.0 |
| Magnesium sulphate |
0.5 |
| Rose-Bengal |
0.05 |
| Agar |
15.5 |
| pH 7.2 ± 0.2 @ 25°C |
Rose-Bengal Chloramphenicol Agar, Oxoid Preparation:
Suspend 16.0g per 500ml (32.0g/l) of distilled water and bring to the boil to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to Rose Bengal Chloramphenicol Agar Base (CM0549) and mix gently. Autoclave at 121°C for 5 minutes. Cool to 50°C, mix gently and pour into Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.
NB: Rose-Bengal photo-oxidises to form toxic compounds. Store plates of the medium in the dark and avoid exposure to light.
Precautions
It is essential to store plates of media containing Rose-Bengal in the dark to prevent toxic photo-oxidation of the dye. See above.
Identify moulds and yeasts by morphological appearance and microscopic examination. Colonies of bacteria and yeasts can be confused.
Rotators
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Biologix
This product is not available at the moment. Please contact us for more information.
These classic adjustable-speed rotators provide continuous end-over-end rolling action to mix samples thoroughly or keep them in suspension. Two types are available.
Features
• Speed range: 0 – 80 rpm
• End-over-end rotation
• Continuous operation
• Compact, space saving design
• Various adapters available for use with 1.5mL – 50mL tubes
RPMI 1640 with L-Glutamine 500ml
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Lonza
Product Overview
RPMI 1640 Medium was developed for culturing normal human leukocytes. It can be used for suspension and monolayer mammalian cultures.
RPMI 1640 Medium will work with different cell lines, it can also be used with primary cells such as blood/hematopoietc cells.
Content
RPMI-1640 Medium
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Sigma-Aldrich
RPMI 1640 Medium, a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. Refer to the table below to choose the right RPMI-1640 for your cell culture!
RPMI-1640 Medium with 20 mM HEPES and L-glutamine, Sigma – Aldrich
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Sigma
Description
Form: Liquid
Synonyms: Roswell Park Memorial Institute 1640 medium
Techniques: Cell culture | mammalian: suitable
Impurities: Endotoxin Tested
pH: >7.2
Components:
HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes
RPMI 1640 Medium is a modification of McCoy’s 5A Medium designed to support lymphoblastoid cells in suspension culture. Although it was originally formulated to be used with a serum supplement, it has since been demonstrated to be capable of supporting a wide range of anchorage-dependent cells. RPMI 1640 Medium has also been extensively used in hybrid cell growth and fusion protocols. It has been shown to be suitable for the culturing of human normal and neoplastic leukocytes. This medium is based on the RPMI-1630 series of media and uses a bicarbonate buffering system with adjustments to amino acid and vitamin levels. It is also used for the growth of various cell cultures, such as fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.
RPMI-1640 Medium-Sigma Aldrich
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Sigma
Description
pH : > 7.2
Components :
HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
RPMI-1640 medium formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. It is used for the culture of human normal and neoplastic leukocytes. RPMI-1640 also supports the growth of various types of cell cultures, like fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.
runDOC
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Cleaver Scientific
runDOC is a portable, lightweight gel documentation system designed exclusively for use with RunVIEW – the innovative new system designed for real-time size fractionation and recovery of nucleic acids. RunDOC comprises a darkroom hood and 12.1 megapixel digital camera to capture images of nucleic acid gels stained with EtBr, SYBR and runSAFE. The RunDOC darkroom hood is placed directly over the RVMSCHOICE gel tank located on the RunVIEW base unit, which includes an integrated power supply and blue LED gel illuminator. The blue LED illuminator provides the excitation source for the DNA gel located within the tank, and following excitation the light emitted is then visualized using either one of the bluVIEW lid options (supplied with RunVIEW and used with the RVMSCHOICE gel tank) or the RunDOC filter slide. RunDOC also benefits from a small footprint area, occupying minimal space within the laboratory.
runSafe
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Cleaver Scientific
RunSAFE is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, CSL-runSAFE is used to prepare DNA markers and samples for loading on agarose or
polyacrylamide gels. It is the most sensitive stain available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process. It is ideal for the environment requiring a safe , non-hazardous alternative to Ethidium Bromide. Storage Store at 4°C up to 12 months. For longer periods, store at -20°C. runSAFE Dye is light sensitive and should be stored protected from light.
runVIEW
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Cleaver Scientific
Using RunVIEW is simple; first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the MSCHOICE tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (loading tier). Replace the lid to connect the MSCHOICE tank to the integrated power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid viewing pane as the samples migrate in real-time to the second row of wells (extraction tier). Once the DNA bands of interest enter the tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.
rvHOOD
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Cleaver Scientific
CSL-RVHOOD is a portable, lightweight viewing hood designed exclusively for use with RunVIEW – the innovative new system designed for real-time size fractionation and recovery of nucleic acids. It comprises a darkroom hood to help aid viewing of samples in a well lit room. The hood is placed directly over the RVMSCHOICE gel tank located on the RunVIEW base unit, which includes an integrated power supply and blue LED gel illuminator. The blue LED illuminator provides the excitation source for the DNA gel located within the tank, and following excitation the light emitted is then visualized using either one of the bluVIEW lid options.
S I M Medium 500g
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Thermo ScientificTM OxoidTM
A medium for the differentiation of enteric bacteria on the basis of sulphide production, indole production and motility.
S S Agar (Modified) 500g
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Thermo ScientificTM OxoidTM
Oxoid SS Agar Modified (Salmonella Shigella Agar Modified) is used for the isolation of Salmonella species and Shigella spp.
Salmonella Shigella Agar Modified, Oxoid Composition
| Typical Formula* |
gm/litre |
| `Lab-Lemco’ powder |
5.0 |
| Peptone |
5.0 |
| Lactose |
10.0 |
| Bile salts |
5.5 |
| Sodium citrate |
10.0 |
| Sodium thiosulphate |
8.5 |
| Ferric citrate |
1.0 |
| Brilliant green |
0.00033 |
| Neutral red |
0.025 |
| Agar |
12.0 |
| pH 7.3 ± 0.2 @ 25°C |
|
Salmonella Shigella Agar Modified, Oxoid Preparation:
Suspend 57g in 1 litre of distilled water. Bring to the boil with frequent agitation, and allow to simmer gently to dissolve the agar. DO NOT AUTOCLAVE. Cool to about 50°C mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
S S Agar 500g
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Thermo ScientificTM OxoidTM
Isolate and identify Salmonella and Shigella species from clinical or food samples with Thermo Scientific™ Oxoid™ SS Agar (Dehydrated). Differentiation of Salmonella and Shigella is achieved by incorporation of lactose in the medium. Organisms which ferment lactose produce acid which, in the presence of the neutral red indicator, results in the formation of red colonies. Salmonella, Shigella, and other non-lactose-fermenting organisms appear as transparent or straw coloured colonies on SS Agar.
SS Agar, Oxoid Composition
| Typical Formula* |
gm/litre |
| `Lab-Lemco’ powder |
5.0 |
| Peptone |
5.0 |
| Lactose |
10.0 |
| Bile salts |
8.5 |
| Sodium citrate |
10.0 |
| Sodium thiosulphate |
8.5 |
| Ferric citrate |
1.0 |
| Brilliant green |
0.00033 |
| Neutral red |
0.025 |
| Agar |
15.0 |
| pH 7.0 ± 0.2 @ 25°C |
SS Agar, Oxoid Preparation:
Suspend 63g in 1 litre of distilled water. Bring to the boil with frequent agitation and allow to simmer gently to dissolve the agar. DO NOT AUTOCLAVE. Cool to about 50°C, mix and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
This medium is highly selective and R-strains of shigellae will not grow on it. It is not recommended for the primary isolation of shigellae.
S10 Streptomycin
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Thermo ScientificTM OxoidTM
S25 Streptomycin
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Thermo ScientificTM OxoidTM
S3 300 Compound Sulphonamide
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Thermo ScientificTM OxoidTM
Sabouraud Dextrose Agar 500g, Oxoid
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Thermo ScientificTM OxoidTM
Identify dermatophytes and other fungi and yeasts in clinical specimens and environmental samples with the acidic Thermo Scientific™ Oxoid™ Sabouraud Dextrose Agar. The low pH supports the growth of fungi while inhibiting bacterial growth in mixed samples. Fungi maintain their typical cultural appearance and, thus, may be readily identified according to standard macroscopic characters.
| Typical Formula * |
gm/litre
|
| Mycological Peptone |
10.0 |
| Glucose (dextrose) |
40.0 |
| Agar |
15.0 |
| pH 5.6 ± 0.2 @ 25°C |
Directions
Add 65g of Sabouraud Dextrose Agar to 1L of distilled water. Sterilize the medium by autoclaving at 121°C for 15 mins. Mix well and pour into a clean petri dish.
Storage Conditions and Shelf Life
Store the medium at 10-30°C and use before the expiry date printed on the label. Store the prepared medium at 2-8°C.
Sabouraud Dextrose Liquid Medium 500g
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Thermo ScientificTM OxoidTM
Oxoid Sabouraud Liquid Medium is an USP formulation for mycological sterility testing.
Sabouraud Liquid Medium, Oxoid Composition
| Formula |
gm/litre |
| Dextrose |
20.0 |
| Pancreatic digest of casein |
5.0 |
| Peptic digest of animal tissue |
5.0 |
| pH 5.6 ± 0.2 @ 25°C |
Sabouraud Liquid Medium, Oxoid Preparation:
Dissolve 30g in 1 litre of water (purified as required). Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away from light between 2-25°C.
Sabouraud Maltose Agar 500g
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Thermo ScientificTM OxoidTM
Oxoid Sabouraud Maltose Agar is used where maltose is the preferred carbohydrate source.
Sabouraud Maltose Agar, Oxoid Composition
| Typical Formula* |
gm/litre |
| Mycological peptone |
10.0 |
| Maltose |
40.0 |
| Agar |
15.0 |
| pH 5.6 ± 0.2 @ 25°C |
Sabouraud Maltose Agar, Oxoid Preparation:
Suspend 65g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour in to sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Some of the pathogenic fungi may produce infective spores which are are easily dispersed into the laboratory. Such organisms should be examined only within a protective cabinet.
The combination of cycloheximide and chloramphenicol inhibits many pathogenic fungi. However, the mycelial phase of Histoplasma capsulatum, Paracoccidioides brasiliensis, Sporothrix schoenckii and Blastomyces dermatitidis is not inhibited by these antibiotics when incubated at 25-30°C .
Note the precautions in handling cycloheximide described in HAZARDS section.
safeVIEW-MINI2
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Cleaver Scientific
The advanced SafeVIEW-MINI2 offers a safe way to view and document gel samples. With a compact design, each transilluminator serves as the perfect workstation for viewing and working with fluorescently-stained nucleic acid gels. The LED 470nm blue light source has the added advantage that it does not cause damage to DNA or RNA that would normally be associated with UV light. safeVIEW-MINI2 Transilluminator has a separate 580nm amber screen and a thinner lightweight casing. With an imaging size of 153 x 153mm, it can be used to view small to medium sized gels and is compatible with multiSUB mini, midi and choice Horizontal DNA gel tanks. safeVIEW-MINI2 is also compatible with microDOC and GDH-BASIC imaging systems.
Saline Tablets, Oxoid
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Thermo ScientificTM OxoidTM
This product is temporarily unavailable. Please contact us for more information.
Oxoid Saline Tablets is used for preparation of 500mL of 0.85% isotonic saline. For Laboratory Use Only.
