Chemical

Description

CAS number: 66-81-9
Chemical Formula: C15H23NO4
Molar Mass: 281.35 g/mol
Synonyms: 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide, Actidione, Naramycin A

Application

In yeast strains, cycloheximide has been used as a protein synthesis inhibitor in the cycloheximide chase experiment. It has been used to inhibit translation in mammalian cells and suppress fungal growth.

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Description

CAS number: 9005-64-5
Synonyms: Polysorbate 20, Polyoxyethylene (20) sorbitan monolaurate, PEG(20)sorbitan monolaurate
Form: Pale yellow liquid

Non-ionic detergent used for immunoprecipitation and for the solubilization of membrane-bound proteins.

Product Specification and MSDS for TWEEN® 20 Detergent (Merck).

Description

CAS number: 1898-66-4
Linear Formula: C₁₈H₁₂N₅O₆
Purity: ≥90% by elemental analysis

DPPH, Merck is a cell-permeable, stable free radical that acts as a hydrogen radical scavenger. Useful in inducing free-radical injury to tissues and as a screening tool for detecting free radical scavenging activity of antioxidants. Absorption max: ~517 nm. Protect the DPPH from light.

Description

Buffer tablets with a pH of 6.8 are recommended for the preparation of buffer solutions according to WEISE for the staining of blood smears. These tablets are used to make buffer solutions and pH-buffered staining solutions, specifically phosphate buffer solutions, which are commonly used in hematology. They are also used to dilute staining solutions and rinse stained samples during the staining process.

100 buffer tablets are sufficient for preparing 100 liters of buffer solution, and each solution can remain stable for up to four weeks. The buffer tablets according to WEISE are in vitro diagnostic products that are CE registered.

Description

CAS number: 540-84-1
Chemical Formula: CH₃C(CH₃)₂CH₂CH(CH₃)CH₃
Molar Mass: 114.23 g/mol
Synonyms: 2,2,4-Trimethylpentane, Isobutyltrimethylmethane, iso-Octane

With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter

Description

CAS number: 77-79-2
Chemical Formula: C4H6O2S
Molar Mass: 118.15 g/mol
Synonyms: 2,5-Dihydrothiophene-1,1-dioxide, 3-Sulfolene

Description

CAS number: 67-56-1
Chemical Formula: CH₃OH
Molar Mass: 32.04 g/mol
Synonyms: MeOH, Hydroxymethane, Methyl alcohol, Carbinol

LiChrosolv® hypergrade is accurately tested for LC-MS suitability, and meet all the requirements of modern LC-MS ionization methods (ESI/APCI – positive and negative mode). Thanks to their low level of ionic background and low ion suppression, LiChrosolv® hypergrade solvents provide high reproducibility and high ionization efficiency. The packaging material has been improved to meet LC-MS quality requirements perfectly. Filtered by 0.2 µm filter.

CAS no.: 7697-37-2, 13138-45-9
Traceability: Traceable to SRM from NIST Ni(NO₃)₂ in HNO₃ 0.5 mol/l 1000 mg/l Ni Certipur®
Synonym: –
Quality level: MQ300
Product specification and SDS for Nicker Standard Solution.

General description

Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Application

Haematology: suitable
Histology: suitable

Histopaque-1077 has been used for-
• the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
• purification of mononuclear cells through centrifugation
• isolation of human lymphocytes
• isolation of breast cancer circulating tumour cells (CTCs) from whole blood

Description

CAS number: 75-09-2
Chemical Formula: CH₂Cl₂
Molar Mass: 84.93 g/mol
Synonyms: DCM, Methylene chloride, Methylene dichloride

With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter

Description

CAS number: 9004-67-5

General description

Methyl-cellulose powder helps to prevent and manage nasal symptoms.

Application

Methyl cellulose has been used:
• in total gastrointestinal transit
• in in vitro motility assay
•  in the measurement of gastric emptying by phenol red method

Description

CAS number: 117-39-5
Chemical Formula: C15H10O7
Molar Mass: 302.24g/mol
Synonyms: 2-(3,4-Dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one, 3,3′,4′,5,6-Pentahydroxyflavone

Application

• Quercetin has been used as an antioxidant which reversed the immunosuppressive effects of high glucose and hyperglycemic sera in type 2 diabetic patients.
• It has been used as a detoxifying phytochemical in Apis mellifera.
• It has been used as a positive control in DPPH (2,2- diphenyl-1-picryhydrazyl) radical scavenging assay. It has also been used for the preparation of calibration curve to determine total flavonoid content.

Description

CAS number: 1313-60-6
Chemical Formula: Na₂O₂
Molar Mass: 77.98 g/mol
Synonyms: –

Product Specification and MSDS for Sodium peroxide for synthesis (Merck).

Description

CAS number: 67-64-1
Chemical Formula: CH₃COCH₃
Molar Mass: 58.08 g/mol
Synonyms: Dimethyl ketone, Propanone, 2-Propanone

With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter.

Description

1X TE Solution, pH 8.0 (10mM TRIS-HCl, 1mM EDTA). Molecular Biology grade.

Specifications:
Conductivity at 25°C: 775-975 µmho
DNase: None detected
pH of solution at 25°C: 7.90-8.10
Protease: None detected
RNase: None detected

MSDS for TE Buffer pH8.0 (Merck).

Description

CAS Number: 7558-79-4
Chemical Formula: Na₂HPO₄
Molecular Weight: 141.96 g/mol
Synonyms: di-Sodium Hydrogen Phosphate, Sodium Hydrogen Phosphate, Sodium Phosphate Dibasic
Quality Level: MQ300

Product Specification and MSDS for di-Sodium Phosphate (Merck).

Description

CAS number: 112-27-6
Chemical Formula: HO(CH2CH2O)2CH2CH2OH
Molar Mass: 150.17g/mol
Synonyms: Triglycol

Description

CAS number: 9005-64-5
Suitability: Suitable for molecular biology
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is a non-ionic detergent that widely used in biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• For lysing mammalian cells at a concentration of 0.005 to 0.5%
• Along with PBS in the dilution of antibodies in immunohistochemistry technique
• For washing cells in FISH (Fluorescence in situ hybridization) technique

Description

Synonyms: Cobaltous nitrate hexahydrate

Formula: Co(NO₃)₂ · 6H₂O

Cobalt (II) nitrate hexahydrate is a red crystal that is soluble in water and most organic solvent. This chemical has many use including cleaning, metal manufacturing and metal surface treatment.

Note:

AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.

Description

CAS number: 9005-64-5
Suitability: Suitable for blocking, cell culture, hybridization, protein purification, transformation
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is a non-ionic detergent that has been used in a variety of applications, including:

• Post-hybridization wash buffer after in situ hybridization.
• Used along with PBS (phosphate-buffered saline) as the washing buffer for ELISA and immunostaining.
• Acted as an emulsifying agent for the preparation of stable oil-in-water emulsions
• Used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
• Acted as a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%.
• Used for lysing mammalian cells at a concentration of 0.005 to 0.5%.