Culture Media Products

Identify dermatophytes and other fungi and yeasts in clinical specimens and environmental samples with the acidic Thermo Scientific™ Oxoid™ Sabouraud Dextrose Agar. The low pH supports the growth of fungi while inhibiting bacterial growth in mixed samples. Fungi maintain their typical cultural appearance and, thus, may be readily identified according to standard macroscopic characters.

Directions
Add 65g of Sabouraud Dextrose Agar to 1L of distilled water. Sterilize the medium by autoclaving at 121°C for 15 mins. Mix well and pour into a clean petri dish.

Storage Conditions and Shelf Life
Store the medium at 10-30°C and use before the expiry date printed on the label. Store the prepared medium at 2-8°C.

Oxoid Sabouraud Liquid Medium is an USP formulation for mycological sterility testing.

Sabouraud Liquid Medium, Oxoid Composition

Sabouraud Liquid Medium, Oxoid Preparation:
Dissolve 30g in 1 litre of water (purified as required). Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away from light between 2-25°C.

Oxoid Sabouraud Maltose Agar is used where maltose is the preferred carbohydrate source.

Sabouraud Maltose Agar, Oxoid Composition

Sabouraud Maltose Agar, Oxoid Preparation:
Suspend 65g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour in to sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Some of the pathogenic fungi may produce infective spores which are are easily dispersed into the laboratory. Such organisms should be examined only within a protective cabinet.
The combination of cycloheximide and chloramphenicol inhibits many pathogenic fungi. However, the mycelial phase of Histoplasma capsulatum, Paracoccidioides brasiliensis, Sporothrix schoenckii and Blastomyces dermatitidis is not inhibited by these antibiotics when incubated at 25-30°C .
Note the precautions in handling cycloheximide described in HAZARDS section.

This product is temporarily unavailable. Please contact us for more information.

Oxoid Saline Tablets is used for preparation of 500mL of 0.85% isotonic saline. For Laboratory Use Only.

Oxoid Salmonella Rapid Test Elective Medium (SRTEM) is a selective enrichment broth medium specifically formulated for use with the Oxoid Salmonella Rapid Test Kit, Part No. FT0201A.

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Composition

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Preparation:
Add 61g to 1 litre of distilled water (double strength medium). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 18-25°C and use as described in the Oxoid Salmonella Rapid Test (Code FT0201) instruction leaflet.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Oxoid Salmonella Selective Supplement is used for the presumptive identification of Salmonella species from clinical and food samples.

• Add to Chromogenic Salmonella Agar Base, Part No. CM1007B or Brilliance™ Salmonella Agar Base, Part No. CM1092B
• Each vial supplements 500mL of medium

Salmonella Selective Supplement, Oxoid Composition

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Enrich and isolate halophilic organisms, especially staphylococci, using Thermo Scientific™ Oxoid™ Salt Meat Broth Tablets. Salt Meat Broth Tablets are specially designed for the isolation of staphylococci from grossly contaminated specimens such as feces, particularly during the investigation of staphylococcal food poisoning. The medium is highly sensitive as it can detect even low numbers of staphylococci from samples having large proportions of heterogeneous microbial flora.^1,2

• Quantity: 100 tablets/bottle

Salt Meat Broth Tablets, Oxoid Composition

Salt Meat Broth, Oxoid Preparation:
Add 2 tablets to 10ml of distilled water in an appropriately sized test tube and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Grow and isolate aerobic and anaerobic organisms of the intestinal tract, which may require selective conditions to inhibit the growth of background organisms like Escherichia coli, with Thermo Scientific™ Oxoid™ Schaedler Anaerobe Agar. The presence of cysteine in the medium provides selectivity against Escherichia coli as cysteine has shown to have inhibitory effects on several enzymatic reactions of Escherichia coli in vitro.¹

Schaedler Anaerobe Agar, Oxoid Composition

Schaedler Anaerobe Agar, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and boil to dissolve the medium completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared plates may be stored at 2-8°C if suitably protected.

• Oxoid Schaedler Anaerobe Broth is a thioglycollate-free broth for the general growth of anaerobes.
• For use in blood cultures and antibiotic MIC studies.

Schaedler Anaerobe Broth, Oxoid Composition

Schaedler Anaerobe Broth, Oxoid Preparation:
Add 26.5g to 1 litre of distilled water and mix to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared broth in the dark at <15°C.

Precautions
As with all anaerobic broth media, it is important to avoid chemo-oxidation (overheating) and photo-oxidation (storage in the light) because such oxidative effects cause the inhibition of growth.

Enrich and isolate Salmonella from feces and food products with Thermo Scientific™ Oxoid™ Selenite Broth Base (Lactose).The presence of Selenium in the medium provides selectivity against non-target bacteria.

Selenite Broth Base (Lactose), Oxoid Composition

Selenite Broth Base (Lactose), Oxoid Preparation:
Dissolve 4g of sodium biselenite LP0121 in 1 litre of distilled water and then add 19g of Selenite Broth Base. Warm to dissolve, mix well and fill out into containers. Sterilise in a boiling water bath, or in free flowing steam, for 10 minutes. DO NOT AUTOCLAVE.

To minimise any possible risk of teratogenicity to laboratory workers, the sodium biselenite must be added as a solution to this medium.
Robertson1 reported miscarriages and possible teratogenic effects on pregnant laboratory assistants which may have been caused by ingested sodium biselenite. Oxoid therefore removed this substance from the powdered medium.

Although no further reports have been received sodium biselenite is now considered to be very toxic and should be handled with great care.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Precautions
Discard the prepared medium if large amounts of reduced selenite can be seen as a red precipitate in the bottom of the bottles.
Do not incubate longer than 24 hours because the inhibitory effect of selenite is reduced after 6-12 hours incubation.
Take sub-cultures of broth from the upper third of the broth column, which should be at least 5 cm in depth.

Enrich and improve the isolation of salmonellae from feces and food samples with Thermo Scientific™ Oxoid™ Selenite Cystine Broth Base. This modified medium is based upon the formulation described by Leifson with added cystine.

Selenite Cystine Broth Base, Oxoid Composition

Selenite Cystine Broth, Oxoid Preparation:
Dissolve 4g of sodium biselenite LP0121 in 1 litre of distilled water and then add 19g of Selenite Cystine Broth Base. Warm to dissolve and dispense into containers. Sterilise by placing in free flowing steam for 15 minutes. DO NOT AUTOCLAVE.

Robertson reported miscarriages and possible tetragenic effects on pregnant laboratory workers which may have been caused by the ingestion of sodium biselenite. To minimise any possible risk of teratogenicity to laboratory workers the sodium biselenite is not included in the dry powder but should be prepared separately as a solution to which the Selenite Cystine Broth Base is added.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Precautions
Observe the precautionary comments made about sodium biselenite in Selenite Broth Base CM0395.
Discard the prepared medium if large amounts of reduced selenite can be seen as a red precipitate in the bottom of the bottle.
Do not incubate longer than 24 hours because the inhibitory effect of selenite is reduced after 6-12 hours incubation.
Take subcultures of broth from the upper third of the broth column which should be at least 5 cm in depth.

This product has been discontinued. The alternative to this product is Sensitest Agar, 500g.

A medium specially designed to give large, clear zones with all antibiotics, without the addition of lysed or whole blood.

Sensitest Agar, Oxoid Composition

Sensitest Agar, Oxoid Preparation:
Suspend 32g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of agar at 2-8°C.

Precautions
As with other susceptibility testing media, Sensitest Agar should be used for rapidly growing aerobic organisms only. It should not be modified by the addition of carbohydrates or incubated in a CO2 enriched atmosphere.
If the medium is used for Bell’s CDS method then the specified discs and technique must be used. Refer to the monograph cited in the References.

A medium specially designed to give large, clear zones with all antibiotics, without the addition of lysed or whole blood.

Sensitest Agar, Oxoid Composition

Sensitest Agar, Oxoid Preparation:
Suspend 32g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of agar at 2-8°C.

Precautions
As with other susceptibility testing media, Sensitest Agar should be used for rapidly growing aerobic organisms only. It should not be modified by the addition of carbohydrates or incubated in a CO2 enriched atmosphere.
If the medium is used for Bell’s CDS method then the specified discs and technique must be used. Refer to the monograph cited in the References.

Cultivate and isolate fastidious microorganisms from clinical samples through improved hemolytic reactions with sheep blood with Thermo Fisher™ Oxoid™ Sheep Blood Agar Base (Dehydrated) when used with sheep blood. Sheep Blood Agar Base was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of organisms without interfering with their hemolytic reactions when used with sheep blood.

Sheep Blood Agar Base (Dehydrated), Oxoid Composition

Sheep Blood Agar Base (Dehydrated), Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 7% sterile sheep blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Simmons Citrate Agar (Dehydrated), Oxoid Composition

Simmons Citrate Agar (Dehydrated), Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is important not to carry over any nutrients into the citrate medium because this will result in false positive tests. Dilute the inoculum in saline before inoculating the citrate medium to avoid a carry-over of other carbon sources.

Oxoid Skim Milk Powder is a thermophile-free, spray dried powder for inclusion in bacteriological culture media.

Detect and enumerate enterococci from water and food samples with Thermo Scientific™ Slanetz and Bartley Medium (Dehydrated). Slanetz and Bartley originally devised this medium to detect and enumerate enterococci by the technique of membrane filtration, but it has also proved useful as a direct plating medium. The medium is very selective for enterococci and, when it is incubated at elevated temperatures (44-45°C), all red or maroon colonies may be accepted as presumptive enterococci.

Slanetz and Bartley Medium (Dehydrated), Oxoid Composition

Slanetz and Bartley Medium Preparation:
Suspend 42g in 1 litre of distilled water and bring to the boil to dissolve the agar completely.

EXCESSIVE HEATING MUST BE AVOIDED. Dispense into Petri dishes and allow to solidify. It should not be remelted. The medium may be used with membrane filters or by spreading dilutions of the sample over the surface of the agar with a glass rod.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Precautions
Count all red, maroon or pink colonies as presumptive enterococci. Not all species reduce TTC therefore pale colonies should not be ignored.
Although incubation at 35°C yields a higher count, it allows the growth of organisms which do not conform to the definition of enterococci. Incubation at 44-45°C has a selective effect and produces fewer false-positives. However, the preliminary incubation at 35°C encourages the recovery of stressed organisms.
Although the selective properties of this medium are very good it is advisable to regard the colony count as a presumptive or unconfirmed count. Further identification may be required depending on the scope of the examination.

Use Thermo Scientific™ Oxoid™ Sodium Biselenite in preparation of Selenite Broth Base (CM0395), Mannitol Selenite Broth Base (CM0399) or Selenite Cystine Broth Base (CM0699).  The complete medium is recommended for selective enrichment of Salmonella species from various samples.

• Dissolve 4 grams in 1 liter of distilled water and use this solution to reconstitute the base medium or tablets.
• Toxic by inhalation and if swallowed. Danger of cumulative effects.
• Not all products are available for sale in all territories. Please inquire.
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Oxoid Sodium Chloride Bacteriological is a dehydrated salt suitable for inclusion in bacteriological culture media.

Carry out microbial limit testing by harmonized methodology of pharmaceutical products in accordance with USP/EP/BP/JP with Thermo Scientific™ Oxoid™ Buffered Sodium Chloride Peptone Solution (Dehydrated). The combination of phosphate buffer, sodium chloride and peptone increases the viability of sensitive microorganisms by providing an isotonic environment. This is of particular importance when trying to recover cells that may be stressed or sensitive to osmotic pressure.

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Composition

Buffered Sodium Chloride Peptone Solution (Dehydrated), Oxoid Preparation:
Suspend 14.63g of Sodium Chloride Peptone Broth (Buffered) in 1 litre of water (purified as required). If required, add 1-10ml Tween® 20 or 80 per litre of medium. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared media at room temperature.

• Oxoid Sorbitol MacConkey Agar is a selective differential medium for the detection of E. coli O157.
• Also available C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar Base, Oxoid Composition

Sorbitol MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C. Pour into sterile Petri dishes.

If required, reconstitute one vial of C-T supplement per 500ml medium, according to the instructions in the product leaflet. Aseptically add this to cooled medium before pouring into sterile Petri dishes

Storage conditions and Shelf life
Dehydrated Sorbitol MaConkey Agar must be stored tightly capped in the original container at 10-30ºC.

Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.

Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

• Oxoid Sorbitol MacConkey Agar w/BCIG is a selective medium containing the chromogenic substrate BCIG.
• Can be used with C-T Supplement, Part No. SR0172E and SR0172H.

Sorbitol MacConkey Agar w/BCIG, Oxoid Composition

Sorbitol MacConkey Agar w/BCIG, Oxoid Preparation:
Suspend 51.6g of SMAC with BCIG in 1 litre of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thermo Scientific™ Oxoid Soy Peptone is obtained by the hydrolysis of soya flour and complies with the USP specification.

Thermo Scientific™ Remel™ Sputasol is a liquifying agent for sputum samples.

• Each vial dilutes in sterile distilled water to make 100mL

Sputasol, Oxoid Composition

Sputasol, Oxoid Preparation:
Aseptically add the contents of one vial (7.5ml) to 92.5ml of sterile distilled water. Use the working solution immediately or store at 2-8°C for upto 48 hours only.