Culture Media Products

Select and identify salmonellae (other than Salmonella Typhi) from food, feed and water samples with Thermo Scientific™ Oxoid™ Modified Brilliant Green Agar (Dehydrated). The modified formula provides improved inhibition of Escherichia coli and Proteus species. The medium has been widely assessed in Europe and has been used in ISO standards.

Brilliant Green Agar (Modified), Oxoid Composition

Brilliant Green Agar (Modified), Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Heat gently with occasional agitation and bring just to the boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C, mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Lactose-fermenting salmonellae may be present in foods.
Salmonella typhi and Shigella species may not grow on this medium.
Proteus, Citrobacter and Pseudomonas species may mimic enteric pathogens by producing small red colonies.

Oxoid Brilliant Green Agar (Kauffmann Medium) is used for increased recovery and selective isolation of Salmonella spp. other than S. Typhi.

• Also available Sulphamandelate Supplement, Part No. SR0087E

Brilliant Green Agar, Oxoid Composition

Brilliant Green Agar, Oxoid Preparation:
Suspend 50g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Lactose-fermenting salmonellae may be present in foods.
Salmonella typhi and Shigella species may not grow on this medium.
Proteus, Citrobacter and Pseudomonas species may mimic enteric pathogens by producing small red colonies.

Oxoid Brilliant Green Bile Broth 2% is used for detection or confirmation of presence of members of coli-aerogenes group.

• Recommended for 44°C confirmatory test for Escherichia coli

Brilliant Green Bile Broth 2%, Oxoid Composition

Brilliant Green Bile Broth 2%, Oxoid Preparation:
Dissolve 40g in 1 litre of distilled water. Mix well, distribute into containers fitted with Durham’s tubes and sterilise by autoclaving at 121°C for 15 minutes.

Do not autoclave double stregnth medium. The alternative procedure is to heat the dissolved broth at 100°C for 30 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared tubes of broth at 2-8°C.

Precautions
Do not autoclave double-strength broth.
Gram-positive sporing organisms may produce gas if the bile/brilliant green inhibition is attenuated by food material.

For the preparation of serum-dextrose-antibiotic medium for the cultivation and isolation of Brucella using Brucella Selective Supplement SR0083 or SR0209. Without antibiotics, it may be used in conjunction with the Cruickshank dyestrip method for differentiation between strains.

Brucella Medium Base, Oxoid Composition

Brucella Medium, Oxoid Preparation:
Suspend 45 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5% of inactivated Horse Serum (i.e. Horse Serum held at 56°C for 30 minutes). Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Oxoid Brucella Selective Supplement is used for the isolation of Brucella spp.

• Add to Blood Agar Base No. 2, Part No. CM0271B; Columbia Blood Agar Base, Part No. CM0331B; or Brucella Medium Base, Part No. CM0169B
• Each vial supplements 500mL of medium

 Brucella Selective Supplement, Oxoid Composition

 Brucella Medium, Oxoid Preparation:
Reconstitute supplement as directed. Incubate for 10-15 minutes at 35°C. Mix thoroughly and immediately add the vial contents to 500 ml of sterile Oxoid Brucella Medium Base, cooled to 50°C together with 5-10% v/v sterile inactivated horse serum SR0035 and 1-5% w/v of a filter-sterilised 10% solution of dextrose. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Oxoid Buffered Listeria Enrichment is selective enrichment medium for the detection of Listeria monocytogenes.

Buffered Listeria Enrichment Broth, Oxoid Composition

Buffered Listeria Enrichment, Oxoid Preparation:
Add 23.5g to 500ml of distilled water and mix well to dissolve. Add the contents of 1 vial of Listeria Selective Enrichment Supplement SR0141 or the alternative Modified Listeria Selective Supplement SR0213 reconstitued as directed. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and aseptically distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Buffered Listeria Enrichment Broth should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored as directed the medium will remain stable until the expiry date printed on the label.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium and is tested in accordance with ISO 6579 and ISO 11133:2014. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water (ISO), Oxoid Composition

Buffered Peptone Water (ISO), Oxoid Preparation:
Add 20.0g of Buffered Peptone Water (ISO) to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Method of use
The sample is added to Buffered Peptone Water (ISO) at a ratio of 1:10, and incubated at 36°C ± 2°C for 16-20 hours before transfer to selective enrichment media.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium and is tested in accordance with ISO 6579 and ISO 11133:2014. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water (ISO), Oxoid Composition

Buffered Peptone Water (ISO), Oxoid Preparation:
Add 20.0g of Buffered Peptone Water (ISO) to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Method of use
The sample is added to Buffered Peptone Water (ISO) at a ratio of 1:10, and incubated at 36°C ± 2°C for 16-20 hours before transfer to selective enrichment media.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water, Oxoid Composition

Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.

Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water, Oxoid Composition

Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.

Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.

Buffered Peptone Water (Dehydrated), CM0509B (Oxoid) is a non-selective pre-enrichment medium that used to pre-enrich food samples prior to the isolation of Salmonella species. It also provides conditions for the resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water, Oxoid Composition

Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.

Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.

Isolate Burkholderia cepacia from respiratory secretions of patients with cystic fibrosis with Thermo Scientific™ Oxoid™ Burkholderia Cepacia Agar Base, which is also used for routine testing of non-sterile inorganic salt solutions in hospital environments. The slower growing B. cepacia can be missed on conventional media, such as blood or MacConkey Agar, due to overgrowth caused by other faster growing organisms found in the respiratory tract of CF patients, such as mucoid Klebsiella species,
Pseudomonas aeruginosa and Staphylococcus species.This may lead to the infection being missed or wrongly diagnosed.

Burkholderia Cepacia Agar Base, Oxoid Composition

Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Oxoid Burkholderia Cepacia Selective Supplement is used for the isolation of Burkholderia cepacia.

• Add to Burkholderia Cepacia Agar Base, Part No. CM0995B
• Each vial supplements 500mL of medium

Burkholderia Cepacia Selective Supplement, Oxoid Composition

Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Isolate, enumerate and differentiate urinary pathogens with Thermo Scientific™ Oxoid™ C.L.E.D. Medium (Dehydrated). Cystine-Lactose-Electrolyte-Deficient (CLED) Medium which provides good colonial differentiation, clear diagnostic characteristics and prevents swarming of Proteus spp. due to the electrolyte deficiency in the formulation.

C.L.E.D. Medium, Oxoid Composition

C.L.E.D. Medium, Oxoid Preparation:
Suspend 36.2g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°Cand use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with Oxoid CampyGen 2.5L Sachet.
• No water or catalyst required.
• No hydrogen gas produced.
• For AnaeroJar™ 2.5L, Part No. AG0025A.

Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.

Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.

Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with the Oxoid CampyGen 3.5L Sachet.

• No water or catalyst required
• No hydrogen gas produced
• For use with Thermo Scientific Oxoid 3.5L Anaerobic Jar, Part No. HP0011A, HP0031A

Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.

Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.

• Produce suitable gaseous atmosphere for growth of microaerophilic microorganisms with the Oxoid CampyGen Compact Sachet.
• For use in Compact Plastic Pouch.
• Each pouch holds 2 standard size petri dishes.
• No water or catalyst required.
• No hydrogen gas produced.
• Use with Compact Plastic Pouch Part No. AG0020C and AnaeroGen™ Compact Sealing Clip, Part No. AN0005C.

Precautions
This product is for in vitro use only
As soon as the CampyGen paper sachet is exposed to air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen paper sachet will not exceed 65°C.

Storage
Store at 2-25°C. Under these conditions, the CampyGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet.

• Oxoid Campylobacter Agar Base is used for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian and environmental specimens.
• Also available Campylobacter Selective Supplement (Blaser-Wang), Part No. SR0098E NMKL; Bolton Broth Selective Supplement, Part No. SR0183E; Modified Bolton Broth Selective Supplement, Part No. SR0208E; Modified Preston Campylobacter Selective Supplement, Part No. SR0204E; and Campylobacter Selective Supplement Preston, Part No. SR0117E.

Campylobacter Agar Base, Oxoid Composition

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Campylobacter Growth Supplement is a liquid supplement for the enhanced growth and aerotolerance of Campylobacter species.

• For the preparation of Blaser-Wang Medium, Bolton Selective Enrichment Broth, Butzler Selective Medium, and Preston Selective Medium
• Each vial supplements 500mL of medium

Campylobacter Growth Supplement, Oxoid Composition

Campylobacter Growth Medium, Oxoid Preparation:
Add the contents of one vial (2 ml) to 500 ml of a sterile nutrient medium cooled to 50-55°C prepared from Oxoid Columbia Agar, Blood Agar Base No.2, or Campylobacter Agar Base, with 5-7% lysed defibrinated horse or sheep blood, and the rehydrated contents of one vial of Campylobacter Antibiotic Supplement SR0069, SR0085 or SR0098. Mix gently and pour aseptically into sterile Petri dishes.

Oxoid Campylobacter Selective Supplement, Blaser-Wang is used to prepare of Blaser-Wang Selective Medium for the isolation of Campylobacter while inhibiting growth of Candida albicans.

• Add to Columbia Blood Agar Base, Part No. CM0271B
• Each vial supplements 500mL of medium

Campylobacter Selective Supplement, Blaser-Wang, Oxoid Composition

Campylobacter Selective Medium, Oxoid Preparation:
Reconstitue one vial as directed add the contents of one vial to 500ml of sterile nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2, with 10% defibrinated horse/sheep blood or 5-7% laked horse blood SR0050, SR0051 or SR0048. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Campylobacter Selective Supplement, Skirrow is used for preparation of Skirrow Selective Medium for the isolation of Campylobacter spp. at 42°C.
• Add to Blood Agar Base No. 2, Part No. CM0271B or Columbia Blood Agar Base, Part No. CM0331B.

Campylobacter Selective Supplement, Skirrow, Oxoid Composition

Campylobacter Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed aseptically add the contents to 500 ml of a nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2 , with 10% defibrinated horse or sheep blood SR0050,SR0051 or 5-7% laked horse blood SR0048. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Cary-Blair Transport Medium is a transport medium for gram-negative and anaerobic microorganisms.

Cary-Blair Transport Medium, Oxoid Composition

Cary-Blair Transport Medium, Oxoid Preparation:
Suspend 13.3g in 1 litre of distilled water and bring gently to the boil to dissolve the agar. Distribute into small, screw-cap bottles and sterilise by immersing in free-steam for 15 minutes. Allow to cool and tighten the screw caps to prevent water loss.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at 2-8°C or at room temperature (22-25°C) up to 19 months.

Precautions
The medium should not be incubated to check sterility, prior to use. This should be carried out on separate quality control samples.
The medium can maintain the viability of fastidious organisms for transport purposes but it should not be used as a storage or enrichment medium.
The results obtained from the medium are dependent on the quality of the specimen material. Commensal anaerobic organisms may overgrow in the medium and cause misleading results.

Oxoid Casein Hydrolysate (Acid) is a purified hydrolysate which provides the necessary nitrogenous material for culture media or can be used when amino acid mixtures are specified.