Culture Media Products

A modified medium requiring the addition of basic fuchsin to form Endo Agar.

• Oxoid Endo Agar is an APHA recommended medium for the confirmation of coliforms.
• Also available Basic Fuchsin Indicator, Part No. BR0050A.

Endo Agar Base, Oxoid Composition

Endo Agar, Oxoid Preparation:
Suspend 36g in 1 litre of distilled water. Add 6ml (or as directed by the supplier) of a 10% w/v solution of basic fuchsin BR0050 (50:50 ethanol/distilled water). Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. Plates should be stored in the dark to preserve their pale pink colour.

BASIC FUCHSIN IS A POTENTIAL CARCINOGEN AND CARE SHOULD BE TAKEN TO AVOID INHALATION OF THE POWDERED DYE AND CONTAMINATION OF THE SKIN.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
Weigh out the basic fuchsin (BR0050) in a fume cupboard and avoid inhalation of the powder or contamination of the skin.
Keep the prepared medium away from light to avoid photo-oxidation.
Endo Agar is quoted by the American Public Health Association as a `Standard Methods’ medium for use in water¹ and dairy products. Windle Taylor recommended the medium for the isolation and differentiation of coli-aerogenes bacteria from water.

Selectively enrich bile tolerant Gram-negative bacteria during microbiological examination of pharmaceutical products with Thermo Scientific™ Oxoid™ Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP).

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Composition

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP), Oxoid Preparation:
Dissolve 25.97g in 1 litre of purified water. Mix well and distribute 100ml into 250ml flasks. Heat the medium at 100°C using free flowing steam for 30 minutes only. Cool rapidly in cold running water.
DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-25°C.

Detect, enumerate and differentiate the members of coliform group with Thermo Scientific™ Oxoid™ Eosin Methylene Blue Agar (Modified) Levine (Dehydrated). This versatile medium, modified by Levine is used for the differentiation of Escherichia coli and Enterobacteria aerogenes and also for rapid identification of Candida albicans. The medium is prepared to the formula specified by the APHA for the detection and differentiation of the coliform group of organisms.

Eosin Methylene Blue Agar (Modified) Levine, Oxoid Composition

Eosin Methylene Blue Agar (Modified) Levine, Oxoid Preparation:
Suspend 37.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidise the methylene blue (i.e. restore its blue colour) and to suspend the precipitate which is an essential part of the medium.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
Further tests are required to confirm the presumptive identity of organisms isolated on this medium. Some strains of Salmonella and Shigella species will not grow in the presence of eosin and methylene blue. Store the medium away from light to prevent photo-oxidation.

**This product is not available at the moment. Please contact us for more information.

Thermo Scientific™ Oxoid Horse Serum is an extract of horse blood for addition to culture media.

Isolate Listeria spp. from food and environmental specimens using Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated). The medium is intended for the isolation of Listeria spp. when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Fraser Broth enrichment cultures should be sub-cultured to plating medium.

Fraser Broth Base, Oxoid Composition

Fraser Broth, Oxoid Preparation:
With Fraser Supplement:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

With Half Fraser Supplement:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers. Alternatively, to make 2.25 litres, use 129.0g of CM0895 and 1 vial of SR0166G.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid Fraser Supplement is used for the isolation of Listeria species.

• Add to Fraser Broth Base, Part No. CM0895B
• Each vial supplements 500mL of medium

Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Selectively isolate Neisseria species with Thermo Scientific™ Oxoid™ GC Agar Base (Dehydrated). GC Agar Base has been formulated to include Special Peptone LP0072 which is a mixture of meat and plant enzymatic digests. The presence of starch in the medium ensures that toxic metabolites produced by Neisseria are absorbed. Phosphate buffers are included to prevent changes in pH due to amine production that would affect the survival of the organism

GC Agar Base, Oxoid Composition

This product is not available at the moment. Please contact us for more information.

Oxoid G-N Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

G-N Anaerobe Selective Supplement, Oxoid Composition

Selective Medium for Gram-Negative Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of G-N Anaerobe Supplement (SR0108) rehydrated as directed, together with 25ml defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

This product has been discontinued. Please contact us for more information.

Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.

GBS Agar Base (Islam), Oxoid Composition

GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.

Oxoid Gelatin Bacteriological is a special bacteriological grade readily soluble in water to give a clear solution free from sulphite and preservatives.

• Firm gel obtained from 15% solution

Oxoid Gentamicin Selective Supplement (256mg/vial) is used for the isolation of Vancomycin-Resistant Enterococci (VRE) and High Level Aminoglycoside-Resistant Enterococci (HLARE) from clinical specimens.

• Add to VRE Agar Base, Part No. CM0985B
• Each vial supplements 500mL of medium

Gentamicin Selective Supplement, Oxoid Composition

Storage conditions and Shelf life
Gentamicin Supplement SR0185 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

An anaerobic enrichment broth for Staphylococcus aureus.

Giolitti-Cantoni Broth, Oxoid Composition

Giolitti-Cantoni Broth, Oxoid Preparation:
Suspend 54.2g in one litre of distilled water and heat gently to dissolve. Dispense 19ml amounts into 20 x 200mm test tubes and sterilise by autoclaving at 121°C for 15 minutes. Cool rapidly then aseptically add to each tube 0.3ml of a sterile solution of Potassium tellurite 3.5% SR0030.
The medium requires the addition of a 3.5% solution of Potassium tellurite when there is a direct addition of 1g of the sample to 19mls of broth. This level of Potassium tellurite is necessary to suppress the high numbers of contaminating organisms that could be expected.
The use of a diluted solution of Potassium tellurite is applicable when a 1 in 10 dilution of the food sample is carried out. In such cases the SR0030 should first be diluted 1 in 10 with sterile distilled water.
The addition of 0.1% Tween 80 can be recommended in order to improve recovery of heat injured Staphylococcus aureus cells e.g. from milk powder. 1g of Tween 80 should be added to 1 litre of CM0523 prior to autoclaving.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid HTM Supplement is used for the susceptibility testing of Haemophilus influenzae.

• Add to HTM Base, Part No. CM0898B Store at -20°C to 0°C
• Each vial supplements 500mL of medium

HTM Supplement, Oxoid Composition

Haemophilus Test Medium (HTM), Oxoid Preparation:
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
HTM Supplement as supplied should be stored in the dark below 0°C. When stored as directed the supplement is stable until the expiry date stated on the label.

• Oxoid Haemophilus Test Medium Base (HTM) is used for the susceptibility testing of Haemophilus influenzae.
• Also available HTM Supplement, Part No. SR0158E. Store at -20° to 0°C.

Haemophilus Test Medium Base (HTM), Oxoid Composition

Haemophilus Test Medium (HTM), Oxoid Preparation:
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
When stored as directed the supplement is stable until the expiry date stated on the label.

• Oxoid Half Fraser Supplement is used for the enrichment of Listeria species.
• Add to Fraser Broth Base, Part No. CM0895B.

Half Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Half Fraser Supplement is used for the enrichment of Listeria species.
• Add to Fraser Broth Base, Part No. CM0895B.

Half Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 129.0g of Fraser Broth in 2.25 litres of distilled water, and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166G reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid Heart Infusion Broth is a highly nutritious liquid medium recommended for the cultivation of fastidious microorganisms.

Heart Infusion Broth, Oxoid Composition

Heart Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121ºC for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium in the dark below 20°C.

Precautions
Heart Infusion Broth should only be used for in vitro diagnostic purposes.

Isolate and differentiate Shigella and Salmonella spp. from enteric pathological specimens with Thermo Scientific™ Oxoid™ Hektoen Enteric Agar (Dehydrated). Hektoen Enteric Agar was developed by King and Metzger. When compared to other selective media this medium gives higher yield of Salmonella and Shigella, while inhibiting accompanying Gram-positive and other microorganisms.

Hektoen Enteric Agar, Oxoid Composition

Hektoen Enteric Agar, Oxoid Preparation:
Suspend 76g of the medium in 1 litre of distilled water and soak for 10 minutes. Heat gently and allow to boil for a few seconds to dissolve the agar. DO NOT AUTOCLAVE. Cool to 50°C and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
Do not overheat the medium or hold it molten for long periods. Proteus species may resemble salmonellae or shigellae.
Further testing must be carried out to confirm the presumptive identification of organisms isolated on this medium.

Oxoid Helicobacter Pylori Selective Supplement (Dent) is for the isolaton of Helicobacter pylori from clinical specimens.

• Used with Columbia Blood Agar Base, Part No. CM0331B
• Each vial supplements 500mL of medium

Helicobacter Pylori Selective Supplement (Dent), Oxoid Composition

Helicobacter Pylori Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Columbia Blood Agar Base cooled to approximately 50°C. Add 35 ml of Laked Horse Blood SR0048 and mix well before pouring into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Hemoglobin Powder Soluble is a specially prepared hemoglobin for use in culture media.

• Oxoid Hoyle Medium Base is used for isolation and differentiation of Corynebacterium diphtheriae Types.
• Requires addition of Potassium Tellurite 3.5%, Part No. SR0030J

Hoyle Medium Base, Oxoid Composition

Hoyle Medium, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 55°C and add 50ml of Laked Horse Blood SR0048 and 10ml of 3.5% Potassium Tellurite solution SR0030, mix, and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
It should be noted that not all corynebacteria produce the typical colonies described above – so in all cases it is advisable to use Hoyle medium in conjunction with the additional media and tests mentioned above.

Detect thermophilic anaerobic organisms causing sulphide spoilage in food with Thermo Scientific™ Oxoid™ Iron Sulphite Agar (Dehydrated). The medium is a modification of Cameron Sulphite Agar developed by the National Canners Association of America1. Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes.

Iron Sulphite Agar, Oxoid Composition

Iron Sulphite Agar, Oxoid Preparation:
Suspend 23.0g in 1 litre of distilled water and boil to dissolve completely. Sterilise by autoclaving for 15 minutes at 121°C. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Precautions
The blackening reaction is only presumptive evidence of clostridial growth. Confirmation tests must be carried out to identify the organism growing in the medium.

Thermo Scientific™ Oxoid Iso-Sensitest Agar is a semi-defined medium designed for antimicrobial susceptibility testing.

Iso-Sensitest Agar, Oxoid Composition

Iso-Sensitest Agar, Oxoid Preparation:
Suspend 31.4g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8° C and use as freshly as possible.

Perform effortless testing using Thermo Scientific™ Oxoid™ Iso-Sensitest Broth (Dehydrated), a semi-defined medium specifically designed for antimicrobial susceptibility testing (AST). Iso-Sensitest Broth is carefully constructed to give reproducible results. It allows the growth of the majority of microorganisms without the need of further supplementation.

Iso-Sensitest Broth, Oxoid Composition

Iso-Sensitest Broth, Oxoid Preparation:
Add 23.4g to 1 litre of distilled water. Mix well and heat to dissolve. Distribute into tubes or bottles. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.