Microbiology

Nutrient agar (500g, Oxoid) is a culture media that used to cultivate and isolate fastidious pathogens and other microorganisms. This non-selective medium contains beef extracts combined with peptone, yeast extracts and sodium chloride to provide the nitrogen compounds, carbon, vitamins and some trace ingredients necessary for the growth of bacteria. A general-purpose medium which may be enriched with up to 10% blood or other biological fluid.

Nutrient Agar, Oxoid Composition

Nutrient Agar, Oxoid Preparation:

Powder: Suspend 28g of nutrient agar in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

A stabilised emulsion of egg yolk for use in culture media. It may be added directly to nutrient media for the identification of Clostridium, Bacillus and Staphylococcus species by their lipase activity.

Egg Yolk Emulsion, Oxoid Directions:
Suspend 21.5g of MYP Agar (CM0929) in 450ml of distilled water and bring gently to the boil to
dissolve. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and
aseptically add 50ml of Egg Yolk Emulsion (SR0047C) and the contents of one vial of Polymyxin B
Supplement (SR0099E) reconstituted as directed. Mix well and pour into sterile Petri dishes.
Alternatively, suspend 8g of Blood Agar Base (CM0055) in 200ml of distilled water and add 2g of
sodium chloride. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and
aseptically add 20ml Egg Yolk Emulsion (SR0047C). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Keep at temperatures between 2° and 8 °C. Use before expiry date on the label.

Precautions
On this medium Bacillus cereus is indistinguishable from Bacillus thuringiensis.
Identify Bacillus cereus by colony form, colour, egg yolk hydrolysis and confirm with cell and spore morphology.
Occasional strains of Bacillus cereus show weak or negative egg yolk reactions.

Brand:

Thermo Scientific^TM Oxoid^TM

A differential supplement for the detection, enumeration, and presumptive identification of Listeria monocytogenes and other Listeria species from food, animal feed and environmental samples according to ISO 11290-1:2017 and ISO 11290-2:2017 standards and other national reference methods using the Ottaviani & Agosti formulation.

Brilliance Listeria Agar (ISO) Differential Supplement, Oxoid Composition

Brilliance Listeria Agar (ISO) Differential Supplement, Oxoid Directions:
Aseptically add 5ml of sterile distilled water to 1 vial and mix gently to dissolve. Aseptically add the vial contents to 480ml of sterile Brilliance™ Listeria Agar base (ISO) (CM1212) prepared as directed, cooled to 48°C and add 1 vial of Brilliance™ Listeria Differential Supplement (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the differential supplements (SR0258) at 2-8°C away from light. Do not freeze the differential supplement solution. Ensure all product is used before the expiry date.

A selective supplement for the detection, enumeration and presumptive identification of Listeria
monocytogenes and other Listeria species from food, animal feed and environmental samples
according to ISO 11290-1:2017 and ISO 11290-2:2017 standards and other national reference
methods using the Ottaviani & Agosti formulation.

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Composition

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Directions:
Aseptically add 1 vial warmed to 48°C to 480ml of sterile Brilliance™ Listeria Agar base (ISO) (CM1212) prepared as directed, cooled to 48°C and add 1 vial of Brilliance™ Listeria Selective Supplement (SR0257E) reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life

Store the selective (SR0257)  supplements at 2-8°C away from light. Ensure all product is used before the expiry date.

Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony.

Brilliance Listeria Agar (ISO), Oxoid Composition

Brilliance Listeria Agar (ISO), Oxoid Preparation:

Suspend 34.5g in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool to 48°C. Aseptically add the contents of 1 vial of Brilliance™ Listeria Selective Supplement (ISO) (SR0257E) reconstituted as directed, and 1 vial of Brilliance™ Listeria Differential Supplement (ISO) (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium (CM1212B) at 10-30°C away from light and ensure bottles are correctly sealed after use.

Store prepared medium for up to 2 weeks at 2-8°C, away from light. Ensure all media is used before the expiry date on the label

Description

This DG 18 (Dichloran glycerol chloramphenicol) agar (base) is tested according to ISO 21527 and FDA-BAM GranuCult®. DG 18 Agar is suitable to enumerate and isolate xenophilic moulds from dried and semi-dried foods such as dried fruits, spices, confectionery, cereals, nuts, and dried meat and fish products.

Description

LB Agar Miller from Merck is a commonly used medium in microbiology laboratories and is suitable for routine bacterial culture and transformation experiments. It is a formulation of Luria-Bertani (LB) agar developed by Miller, which includes a mixture of peptides, amino acids, and vitamins to support the growth of a wide range of bacteria.

Application

For maintenance of Lactobacilli and for determining the action of bacteria on milk.

Final pH: 6.8±0.2 (25 °C)

Oxoid Listeria Selective Enrichment Supplement, Modified is used for the enrichment of Listeria spp.

• Modified with 10mg/L acriflavin
• Add to Listeria Enrichment Broth, Part No. CM0862B
• Each vial supplements 2.25L of medium

Listeria Selective Enrichment Supplement (Modified), Oxoid Composition

Listeria Selective Enrichment Medium Preparation:
Aseptically add 10ml of distilled water to one vial and invert gently to dissolve. Add the vial contents to 2.25 litres of Listeria Enrichment Broth Base. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required .

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product has been discontinued, please refer to SR0258E

Thermo Scientific™ Oxoid OCLA (ISO) Differential Supplement is a differential supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Differential Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid ONE Broth Salmonella Selective Supplement is a selective supplement for use with ONE Broth-Salmonella Base.

ONE Broth Salmonella Selective Supplement, Oxoid Composition

ONE Broth Salmonella Selective Medium Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
The ONE Broth-Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Thermo Scientific™ Oxoid XLT-4 Selective Supplement is a selective supplement for use with XLT-4 Agar.

XLT-4 Selective Supplement, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid T.T.C. Solution is a solution for the addition to KF Streptococcus Agar for the enumeration of entercocci.

T.T.C. Solution, Oxoid Composition

Medium Preparation:
Aseptically add the contents to 500ml of KF Streptococcus Agar CM0701 cooled to approximatly 50°C. Mix gently and pour into sterile Petri dishes.

This product has been discontinued, please refer to SR0257E

Thermo Scientific™ Oxoid OCLA (ISO) Selective Supplement is a selective supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Selective Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid PALCAM Supplement is a selective supplement for use with PALCAM Agar Base.

PALCAM Supplement, Oxoid Composition

PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.

The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.

Thermo Scientific™ Oxoid Listeria Primary Enrichment Supplement (UVMI) is a primary selective enrichment supplement for the isolation of listeria when used with Listeria Enrichment Broth Base (UVM Formulation) CM0863.

Oxoid Listeria Primary Enrichment Supplement (UVMI), Oxoid Composition

Listeria Primary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

A Bacillus cereus 569/H9 lactamase (E.C.5.2.6) presented as a freeze-dried powder containing buffer salts. Each vial contains a 3,300lU of activity (1 unit of enzyme activity will hydrolyse 1.0μmol of benzylpenicilloic acid per minute pH7.0 and at 25°C).

Used for the inactivation of susceptible beta-lacram antibiotics in preparations, blood or tissue samples.