Microbiology

Thermo Scientific™ Oxoid Legionella BMPA-α Selective Supplement is a selective supplement for the isolation of legionellae when used with Legionella BCYE Growth Supplement (SR0010) and Legionella CYE Agar Base (CM0655).

Legionella BMPA-α Selective Supplement, Oxoid Composition

Legionella BMPA-α Selective Medium Preparation:
Reconstitute one vial SR0111E per 100ml medium as directed. Aseptically add the vial contents to BCYE-a Medium prepared from Legionella CYE Agar Base (CM0655) and Legionella BCYE Supplement (SR0110) and cooled to 50°C. Mix well and pour into sterile Petri dishes.

Thermo Scientific™ Oxoid Egg Yolk Tellurite Emulsion is an emulsion of egg yolk containing potassium tellurite for use in Baird-Parker Medium CM0275 and Baird-Parker Medium (ISO) CM1127.

Thermo Scientific™ Oxoid Defibrinated Sheep Blood is a blood product.

**This product is not available at the moment.

Thermo Scientific™ Oxoid Defibrinated Horse Blood is a blood product.

**This product is not available at the moment.

Thermo Scientific™ Oxoid Defibrinated Horse Blood is a blood product.

**This product is not available at the moment.

Thermo Scientific™ Oxoid Potassium Lactate is a solution for addition to culture media, Lysine Medium CM0191 for example.

Thermo Scientific™ Oxoid Lactic Acid is a solution for addition to culture media, primarily to lower the pH.

Thermo Scientific™ Oxoid Lactic Acid is a solution for addition to culture media, primarily to lower the pH.

Thermo Scientific™ Oxoid Soy Peptone is obtained by the hydrolysis of soya flour and complies with the USP specification.

Thermo Scientific™ Oxoid MOPS-BLEB Broth Base is a medium for using with BAX Listeria species.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium and is tested in accordance with ISO 6579 and ISO 11133:2014. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water (ISO), Oxoid Composition

Buffered Peptone Water (ISO), Oxoid Preparation:
Add 20.0g of Buffered Peptone Water (ISO) to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Method of use
The sample is added to Buffered Peptone Water (ISO) at a ratio of 1:10, and incubated at 36°C ± 2°C for 16-20 hours before transfer to selective enrichment media.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Thermo Scientific™ Oxoid Brilliance Bacillus Cereus Agar is a chromogenic medium for the isolation and differentiation of Bacillus cereus from food samples.

Brilliance Bacillus Cereus Agar Base, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

Precautions
X-glucopyranoside negative B. cereus strains may present white colonies on this medium due to poor or no utilisation of the chromogen.

Thermo Scientific™ Oxoid Potato Dextrose Broth is a general broth for yeasts and molds.

Thermo Scientific™ Oxoid Brilliance™ UTI Agar (formerly Chomogenic UTI Agar ) is a chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections (UTIs).

Brilliance UTI Agar, Oxoid Composition

Brilliance UTI Agar, Oxoid Preparation:
Suspend 56.3g of Brilliance UTI Agar in 1 litre of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before pouring plates.

Storage conditions and Shelf life
Dehydrated Brilliance UTI Agar must be stored tightly capped in the original container at 10-30ºC.
Oxoid Brilliance UTI Agar plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light.

When stored as directed, the unopened product will remain stable until the expiry date on the label. Locally prepared media can be stored for up to 2 weeks when made from CM0949 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

This product has been discontinued. The alternative to this product is Sensitest Agar, 500g.

A medium specially designed to give large, clear zones with all antibiotics, without the addition of lysed or whole blood.

Sensitest Agar, Oxoid Composition

Sensitest Agar, Oxoid Preparation:
Suspend 32g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of agar at 2-8°C.

Precautions
As with other susceptibility testing media, Sensitest Agar should be used for rapidly growing aerobic organisms only. It should not be modified by the addition of carbohydrates or incubated in a CO2 enriched atmosphere.
If the medium is used for Bell’s CDS method then the specified discs and technique must be used. Refer to the monograph cited in the References.

Thermo Scientific™ Oxoid Mannitol Selenite Broth Base is a modification of Selenite F Broth for the selective enrichment of salmonellae.

Mannitol Selenite Broth Base, Oxoid Composition

Mannitol Selenite Broth, Oxoid Preparation:
Add 19g to 1 litre of distilled water to which 4g of sodium biselenite LP0121 has been added.
Warm to dissolve, mix well and fill out into containers. Sterilise in a boiling water bath, or in free flowing steam, for 10 minutes.
DO NOT AUTOCLAVE.

To minimise any possible risk of teratogenicity to laboratory workers, the sodium biselenite must be added to this medium separately.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.

Thermo Scientific™ Oxoid Thioglycollate Medium is tested according to EP/USP.

Thioglycollate Medium, Oxoid Composition

Thioglycollate Medium, Oxoid Preparation:
Suspend Thioglycollate medium (29.75 grams/ 1itre) in de-ionised water. Heat to dissolve. Cool and dispense 100ml into final containers, 100ml DIN bottles. Sterilise at 121°C for 15 minutes. When cooled label each bottle and pack in units of 10 into labelled boxes.

Storage conditions and Shelf life
Store away from light between 2-25ºC. Product shelf life from date of production is 1 year.

Thermo Scientific™ Oxoid Tryptone Soya Broth is tested according to EP/USP.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Thermo Scientific™ Oxoid Vegetable Peptone No. 1 is a highly nutritious, meat-free general purpose peptone for growth of bacteria and fungi.