Microbiology

Oxoid Membrane Clostridium Perfringens Selective Supplement (m-CP) is a chromogenic medium for rapid and presumptive identification of Clostridium perfringens from water samples.

• Add to Membrane Clostridium Perfringens (m-CP) Agar Base, Part No. CM0992B
• Each vial of SR0188E supplements 500mL of medium

1M-Cp Selective Supplement, Oxoid Composition

1M-Cp Selective Supplement, Oxoid Preparation:

Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:

Storage conditions and Shelf life
m-CP Selective Supplement SR0188 should be stored at 2-8°C.
Store the prepared medium at 2-8°C.

Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.

• Each vial supplements 500mL of medium
• Format: Freeze dried

Vancomycin Supplement, Oxoid Composition

Storage conditions and Shelf life
Vancomycin Supplement SR0186 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Oxoid Gentamicin Selective Supplement (256mg/vial) is used for the isolation of Vancomycin-Resistant Enterococci (VRE) and High Level Aminoglycoside-Resistant Enterococci (HLARE) from clinical specimens.

• Add to VRE Agar Base, Part No. CM0985B
• Each vial supplements 500mL of medium

Gentamicin Selective Supplement, Oxoid Composition

Storage conditions and Shelf life
Gentamicin Supplement SR0185 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Oxoid Meropenem Selective Supplement (1mg/vial) is used for the isolation of Vancomycin-Resistant Enterococci (VRE) and HighLevel Aminoglycoside-Resistant Enterococci (HLARE) from clinical specimens.

• Add to VRE Agar Base, Part No. CM0984B
• Each vial supplements 500mL of medium

Meropenem Selective Supplement, Oxoid Composition

Storage conditions and Shelf life
Meropenem Supplement SR0184 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Oxoid Bolton Broth Selective Supplement is used for the selective pre-enrichment of Campylobacter spp. in food samples.

Add to to Bolton Broth, Part No. CM0983B. Each vial supplements 500mL of medium.

Bolton Broth Selective Supplement, Oxoid Composition

Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.

Oxoid Novobiocin Selective Supplement (10mg/vial) is added to EC Broth, Part No. CM0990B; Muller-Kauffmann Tetrathionate-Novobiocin Broth Base, Part No. CM0343B; Brilliant Green Agar, Part No. CM0263B; MRSV, Part No. CM0910B; Modified Tryptone Soya Broth, Part No. CM0989B; and Lysine Iron Agar, Modified, Part No. CM0886B.

• Each vial supplements 500mL of medium

Novobiocin Supplement, Oxoid Composition

Novobiocin Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to the designated medium cooled to approximately 50°C. Mix well and dispense into sterile containers.

Storage conditions and Shelf life
Novobiocin Supplement SR0181 should be stored in the dark at 2-8°C. When stored as directed, the supplement remains stable until the expiry date printed on the label.

Oxoid Legionella BCYE Supplement w/o L-cysteine is used for the isolation of Legionella spp.

• Add to Legionella CYE Agar Base, Part No. CM0655B
• Each vial supplements 100mL of medium

Legionella BCYE Growth Supplement without L-cysteine, Oxoid Composition

Legionella BCYE Growth Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90ml of sterile Legionella CYE Agar Base (CM0655) cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid CDMN Supplement is used for the growth of Clostridium difficile.

• Add to to C. Difficile Agar Base, Part No. CM0601B
• Each vial for 2L medium

CDMN Selective Supplement, Oxoid Composition

CDMN Agar, Oxoid Preparation:
Aseptically add 2ml of sterile distilled water to one vial of supplement and mix gently to dissolve completely. Aseptically add the contents to 500ml of sterile Clostridium difficile Agar Base CM0601 cooled to approximately 50°C together with 7% (v/v) of Defibrinated Horse Blood SR0050. Mix well and pour the resulting CDMN Agar into sterile Petri dishes.

• Oxoid C-T Supplement is a chromogenic medium for the identification of Escherichia coli O157:H7.
• Add to Sorbitol MacConkey Agar, Part No. CM0813B or to Sorbitol MacConkey Agar with BCIG, Part No. CM0981B.

Cefixime Tellurite Selective Supplement, Oxoid Composition

Sorbitol MacConkey Agar, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Sorbitol MacConkey Agar Base CM0813 cooled to approximately 50°C. Mix well and pour the medium into Petri dishes.

Storage conditions and Shelf life
Cefixime-Tellurite Supplement (SR0172) should be stored in the dark at temperatures below 0°C.

Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.

Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

• Oxoid Half Fraser Supplement is used for the enrichment of Listeria species.
• Add to Fraser Broth Base, Part No. CM0895B.

Half Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 129.0g of Fraser Broth in 2.25 litres of distilled water, and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166G reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Half Fraser Supplement is used for the enrichment of Listeria species.
• Add to Fraser Broth Base, Part No. CM0895B.

Half Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid Neomycin Selective Supplement (150mg/vial) allows the growth of clostridia and other anaerobes.

• Add to Blood Agar, Wilkins-Chalgren Anaerobe Agar, Part No. CM0619B, or Columbia Blood Agar, Part No. CM0331B
• Each vial supplements 2L of medium

Neomycin Selective Supplement, Oxoid Composition

Neomycin Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 2 litres of Wilkins Chalgren Agar Base CM0619 or another appropriate medium cooled to approximately 50° C. Mix well and pour into sterile Petri dishes.

Oxoid MRSV Selective Supplement is used for the detection of Salmonella spp. from food and environmental samples.

• Add to MSRV Medium Base, Part No. CM0910B and Lysine Iron Agar, Modified (MLIA), Part No. CM0886B
• Each vial supplements 500mL of medium

MRSV Selective Supplement, Oxoid Composition

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Oxoid HTM Supplement is used for the susceptibility testing of Haemophilus influenzae.

• Add to HTM Base, Part No. CM0898B Store at -20°C to 0°C
• Each vial supplements 500mL of medium

HTM Supplement, Oxoid Composition

Haemophilus Test Medium (HTM), Oxoid Preparation:
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
HTM Supplement as supplied should be stored in the dark below 0°C. When stored as directed the supplement is stable until the expiry date stated on the label.

Oxoid Fraser Supplement is used for the isolation of Listeria species.

• Add to Fraser Broth Base, Part No. CM0895B
• Each vial supplements 500mL of medium

Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid CCDA Selective Supplement is used for the isolation of Campylobacter spp., especially C. upsaliensis or Arcobacter.

• For the isolation of Arcobacter spp., add to Arcobacter Broth Base, Part No. CM0965B
• For the isolation of Campylobacter spp., especially C. upsaliensis, add to Campylobacter Blood-Free Selective Agar Base (CCDA Preston), Part No. CM0739B

CCDA Selective Supplement, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Oxoid CCDA Selective Supplement is used for the isolation of Campylobacter spp., especially C. upsaliensis or Arcobacter.

• For the isolation of Arcobacter spp., add to Arcobacter Broth Base, Part No. CM0965B
• For the isolation of Campylobacter spp., especially C. upsaliensis, add to Campylobacter Blood-Free Selective Agar Base (CCDA Preston), Part No. CM0739B

CCDA Selective Supplement, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Oxoid Legionella GVPC Selective Supplement is used for the isolation of Legionella spp. from environmental samples.

• Add to Legionella CYE Agar Base, Part No. CM0655B
• Each vial supplements 500mL of medium

Legionella GVPC Selective Supplement, Oxoid Composition

Legionella GVPC Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Legionella CYE Agar Base CM0655 to which one vial of Legionella BCYEa Legionella Growth Supplement SR0110C has been added cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid STAA Selective Supplement is used for the isolation of Brochothrix thermosphacta from food samples.

• Add to STAA Agar Base, Part No. CM0881B
• Each vial supplements 500mL of medium

STAA Selective Supplement, Oxoid Composition

STAA Selective Medium Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
STAA Selective Supplement SR0151 contains cycloheximide and thallous acatate and is toxic if swallowed, inhaled or if in contact with skin.

• Oxoid TTC Solution (0.125%) is used for the enumeration and identification of coliform bacteria.
• Add to Tergitol 7 Agar, Part No. CM0793B.
• Each vial for 100mL medium.

Oxoid Helicobacter Pylori Selective Supplement (Dent) is for the isolaton of Helicobacter pylori from clinical specimens.

• Used with Columbia Blood Agar Base, Part No. CM0331B
• Each vial supplements 500mL of medium

Helicobacter Pylori Selective Supplement (Dent), Oxoid Composition

Helicobacter Pylori Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Columbia Blood Agar Base cooled to approximately 50°C. Add 35 ml of Laked Horse Blood SR0048 and mix well before pouring into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Listeria Secondary Selective Enrichment Supplement, UVMII is used in a two-step selective enrichment (USDS-FSIS) method for the enrichment of Listeria spp.

• Add to Listeria Enrichment Broth Base (UVM formulation), Part No. CM0863B
• Each vial supplements 500mL of medium

Listeria Secondary Selective Enrichment Supplement (UVMII), Oxoid Composition

Listeria Secondary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

Oxoid Listeria Selective Enrichment Supplement is used for the enrichment of Listeria spp.

• Add to Listeria Enrichment Broth, Part No. CM0862B
• Each vial supplements 500mL of medium

Listeria Selective Enrichment Supplement, Oxoid Composition

Listeria Selective Enrichment Medium Preparation:
Reconstitute one vial as directed, and add the contents to 500ml of  Listeria Enrichment Broth Base (CM0862). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before distributing into sterile containers in volumes as required.

Storage conditions and Shelf life
Listeria Selective Enrichment Supplement SR0141 as supplied should be stored at 2-8°C. When stored as directed the reagents are stable until the expiry date printed on the label.

Precautions
Listeria Selective Enrichment Supplement contains cycloheximide and is toxic if swallowed, inhaled or by skin contact. As a precaution when handling wear gloves and eye/face protection.
Acriflavine is activated by light and may form compounds inhibitory for Listeria.