Microbiology

Oxoid MLCB Agar is used for the selective isolation of Salmonella spp., not S.Typhi or S. Paratyphi A.

MLCB Agar, Oxoid Composition

MLCB Agar, Oxoid Preparation:
Suspend 49.0g in 1 litre of distilled water. Mix and bring gently to the boil with frequent agitation to dissolve the medium completely. Cool to 50°C and pour approximately 20ml into sterile Petri dishes.
DO NOT AUTOCLAVE OR OVERHEAT.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The identity of colonies presumed from their appearance to be Salmonella spp. must be confirmed by biochemical and serological testing. In common with other enteric media care must be taken to ensure the purity of colonies taken for further testing as organisms that are inhibited from developing into colonies remain viable and may accidentally be picked on sub-culture.

Detect, enumerate and differentiate Escherichia coli and other coliforms by single membrane filtration with Thermo Scientific™ Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) (Dehydrated). The presence of lauryl sulphate and lactose with phenol red allow the selective isolation and differentiation of Escherichia coli and coliforms respectively.

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Composition

Membrane Lactose Glucuronide Agar (MLGA), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium may be stored for up to 1 week at 2-8°C.

Oxoid Modified Bolton Broth Selective Supplement is for the selective pre-enrichment of Campylobacter spp. in food samples.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Bolton Broth, Part No. CM0983B
• Each vial supplements 500mL of medium

Modified Bolton Broth Selective Supplement, Oxoid Composition

Modified Bolton Broth Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Bolton Broth CM0983 cooled to approximately 50°C with 25 ml of laked horse blood SR0048. Mix well and distribute into sterile containers.

Oxoid Modified Brucella Selective Supplement is for the cultivation and isolation of Brucella spp.

• Alternative supplement containing natamycin as a replacement for cycloheximide
• Add to Blood Agar Base No. 2, Part No. CM0271B or Brucella Medium Base, Part No. CM0169B
• Each vial supplements 500mL of medium

Modified Brucella Selective Supplement, Oxoid Composition

Modified Brucella Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml Brucella Medium Base, Columbia Agar Base or Blood Agar Base No.2 together with 5-10% v/v inactivated horse serum SR0035 and 1-5% w/v sterile solution of glucose, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Oxoid Modified Karmali Selective Supplement is for a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42° C.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Campylobacter Agar Base (Karmali), Part No. CM0935B
• Each vial supplements 500mL of medium

Modified Karmali Selective Supplement, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Campylobacter Agar Base (Karmali) CM0935 cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Selectively enrich Cronobacter spp. from milk and milk samples using Thermo Scientific™ Oxoid™ Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated). Modified Lauryl Sulphate Tryptose Broth Base was originally described by Guillaume-Gentil et al. for the selective enrichment of Cronobacter spp. The high salt concentration of the medium restricts the growth of other members of Enterobacteriaceae and the combination of vancomycin and sodium lauryl sulphate inhibits the growth of Gram-positive organisms commonly isolated in production environments.

Modified Lauryl Sulphate Tryptose Broth Base, Oxoid Composition

Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label
Where possible the complete medium should be prepared on the day of testing and stored for no longer than 1 day at 0-5ºC

• Oxoid Listeria Selective Supplement (Oxford) is used for the detection of Listeria monocytogenes.
• Add to Listeria Selective Agar Base, Part No. CM0856B.

Modified Listeria Selective Supplement (Oxford), Oxoid Composition

Modified Listeria Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to500 ml of sterilised Listeria Selective Agar Base (Oxford Formulation) CM0856, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Oxoid Modified Preston Campylobacter Selective Supplement is for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian, and environmental samples.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Campylobacter Agar Base, Part No. CM0689B or Nutrient Broth No. 2, Part No. CM0067B
• Each vial supplements 500mL of medium

Modified Preston Campylobacter Selective Supplement, Oxoid Composition

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

• Oxoid Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium) is used for the detection of motile Salmonella spp. from food and environmental samples.
• Also available MSRV Selective Supplement, Part No. SR0161E and Novobiocin Supplement, Part No. SR0181E.

Modified Semi-Solid Rappaport-Vassiliadis Medium Base (MSRV Medium), Oxoid Composition

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Precautions
The basal medium is very hygroscopic. When handling the powder a face mask and gloves must be worn, refer to the material safety data sheet.

Thermo Scientific™ Oxoid MOPS-BLEB Broth Base is a medium for using with BAX Listeria species.

Isolate and enumerate lactic acid bacteria from food with Thermo Scientific™ Oxoid™ MRS (ISO) Agar (DeMan, Rogosa and Sharpe) (Dehydrated) while adhering to ISO 15214: 1998. This medium is designed to support the growth of all strains of Lactobacilli, even the difficult and slow-growing strains of Lactobacillus brevis and Lactobacillus fermenti.

MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Composition

MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Preparation:
Suspend 66.73g in 1 litre of distilled water. Boil to dissolve the medium completely. Dispense into tubes, bottles or flasks and sterilize by autoclaving at 121°C for 15 minutes. Selectivity can be altered by pH adjustment.

Storage conditions and Shelf life
Store the dehydrated medium at 10–30°C and use before the expiry date on the label.
Store the prepared plates at 2–8°C.

Precautions
Sterilization of this medium in some autoclaves, at 121°C for 15 minutes, may cause the medium in some cases to fall outside the specified pH limit of 5.7 ± 0.1. Adjustment of the medium in these rare cases is recommended using acetic acid or sodium hydroxide.

Oxoid MRS Agar (de Man, Rogosa, Sharpe) (Dehydrated) is used for the growth of Lactobacilli.

Composition of MRS Agar, Oxoid

Preparation of MRS Agar, Oxoid

Suspend 62g of your MRS Agar in 1 litre of distilled water. You can boil to dissolve the powder completely. After a dissolve, you can sterilise the medium by autoclaving at 121°C for 15 minutes.

Storage Conditions and Shelf Life of MRS Agar, Oxoid

You can store your dehydrated MRS Agar at 10-30°C and use before the expiry date on the label. You can store the prepared plates at 2-8°C.

Oxoid MRSV Selective Supplement is used for the detection of Salmonella spp. from food and environmental samples.

• Add to MSRV Medium Base, Part No. CM0910B and Lysine Iron Agar, Modified (MLIA), Part No. CM0886B
• Each vial supplements 500mL of medium

MRSV Selective Supplement, Oxoid Composition

MSRV Medium, Oxoid Preparation:
Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.
DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Oxoid MRVP Medium. A glucose-phosphate medium is used for the differentiation of the coli-aerogenes group by the Methyl Red and Voges-Proskauer tests.

MRVP Medium, Oxoid Composition

MRVP Medium, Oxoid Preparation:
Add 17g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-6°C.

Precautions
The MR-VP reactions are only part of the tests required to identify organisms.
Each laboratory should standardise on the inoculum density, volume of broth and the test container size.
MR tests require a minimum incubation of 48 hours before the pH indicator is added.
When using Barritt’s reagents add a-naphthol first and KOH second; do not reverse this order.
Vaughn et al. warned of false positive VP reactions if the completed tests are left standing for over an hour.

Selectively enrich Salmonella spp. from food, animal feces, and environmental samples with Thermo Scientific™ Oxoid™ Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO) (Dehydrated). The efficacy of the medium is based upon the ability of Salmonellae to migrate through the selective medium ahead of competing Gram-negative, motile organisms and, thus, produce opaque halos of growth.

Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO), Oxoid Composition

MSRV (ISO) Agar, Oxoid Preparation:
Suspend 31.6g of MSRV (ISO) Agar base in 1 litre of distilled water. Bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool to 50°C, and aseptically add the contents of 1 vial of Novobiocin Supplement (SR0181), reconstituted as directed. Mix well, and pour into sterile Petri dishes. This medium is semi-solid, therefore do not invert plates.

Storage conditions and Shelf life
MSRV (ISO) should be stored in the tightly capped original container at 10-30°C. Novobiocin Supplement should be stored in the dark at 2-8°C. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Precautions
MSRV (ISO) Agar is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.
Basal medium is very hygroscopic.
When handling powder a face mask and gloves must be worn. Refer to material safety data sheet for details.

Perform effortless Antimicrobial Susceptibility Testing (AST) using Thermo Scientific™ Oxoid™ Mueller Hinton Agar (Dehydrated), a medium designed and manufactured according to the European standard, EUCAST and the American standard, CLSI.

Composition of Mueller Hinton Agar, Oxoid

Preparation of Mueller Hinton Agar, Oxoid
Add 38g of Mueller Hinton Agar to 1 litre of distilled water. Bring to the boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life:
Store the dehydrated Mueller Hinton Agar, Oxoid at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton Agar plates at 2-8°C.

Precautions
Incubation in a carbon dioxide enriched atmosphere is not recommended because of its pH effect on the medium. If it is imperative to use CO₂ then known control organisms should be included with the test plates to measure its effect.
Carbohydrates should not be added to Mueller-Hinton Agar because they may influence the rate of growth of the organism and the resulting pH of the medium.
The addition of lysed horse blood to the medium may further reduce the levels of thymidine and prevent the growth of thymidine-dependent organisms.

The medium may not support the growth of some fastidious strains for example microorganisms that require thymine and thymidine for growth. Close adherence to the methodology is required to obtain satisfactory results.

Mueller Hinton Broth, Oxoid conforms to international standards for antimicrobial susceptibility testing media.

Mueller Hinton Broth, Oxoid Preparation
Place 21.0g of Mueller Hinton Broth, Oxoid in 1 litre of distilled water mix to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Chill and adjust cation levels if necessary.

Storage conditions and Shelf life
Store the dehydrated Mueller Hinton broth at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton broth at 2-8°C.

Precautions
Monitor the performance of the broth routinely using the standard QC organisms. If the broth does not yield the expected MIC values, modify the volumes of Mg++ and Ca++ solutions.

If the thymidine content is lowered, after the addition of lysed horse blood or thymidine phosphorylase, the MIC values may be lower.

Oxoid Muller-Kauffmann Tetrathionate Broth Base is selective enrichment medium for isolation of Salmonella spp. and suppression of Proteus species.

Muller-Kauffmann Tetrathionate Broth Base, Oxoid Composition

Muller-Kauffmann Tetrathionate Broth, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil. Cool below 45°C and add, just prior to use, 19ml of iodine solution and 9.5ml of a 0.1% brilliant green solution. Mix well and fill out into sterile tubes or flasks.

Dissolve the potassium iodide in approximately 5ml of distilled water, add the iodide and gently warm the solution to completely dissolve it. Make up the volume to 100ml with distilled water.

Add the brilliant green to the distilled water and shake to dissolve the dye. Heat the solution to 100°C for 30 minutes and shake from time to time whilst cooling, to ensure that the dye has completely dissolved. Store in a brown glass bottle or away from light.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not autoclave the base broth.
Add the iodine solution and brilliant green just prior to use.
The medium is not suitable for the growth of Salmonella typhi, Salmonella sendai, Salmonella pullorum and Salmonella gallinarum.