Microbiology

Nutrient agar (500g, Oxoid) is a culture media that used to cultivate and isolate fastidious pathogens and other microorganisms. This non-selective medium contains beef extracts combined with peptone, yeast extracts and sodium chloride to provide the nitrogen compounds, carbon, vitamins and some trace ingredients necessary for the growth of bacteria. A general-purpose medium which may be enriched with up to 10% blood or other biological fluid.

Nutrient Agar, Oxoid Composition

Nutrient Agar, Oxoid Preparation:

Powder: Suspend 28g of nutrient agar in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Nutrient Agar Tablets make 5mL of medium each.

Nutrient Agar Tablets, Oxoid Composition

Nutrient Agar, Oxoid Preparation:
Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Cultivate fastidious pathogens and other microorganisms with nutritious Thermo Scientific™ Oxoid™ Nutrient Broth No.2 (Dehydrated). This medium is comparable to a meat infusion and is richer in nutrients than Nutrient Broth (CM0001). It gives good growth from small inocula and is recommended for sterility testing for aerobic organisms. It complies with the recommendations in the ‘British Pharmacopoea’ for the composition of a sterility testing medium for aerobes.

Nutrient Broth No.2, Oxoid Composition

Nutrient Broth No.2, Oxoid Preparation:
Add 25g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Nutrient Gelatin is used for the differentiation of microorganisms by their proteolytic effects.

Nutrient Gelatin, Oxoid Composition

Nutrient Gelatin, Oxoid Preparation:
Suspend 128g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring and cool below 20°C or leave to set in a refrigerator.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not shake the gelatin tubes whilst they are warm because growth and liquefaction of gelatin frequently occurs on the surface layer only.
In routine diagnostic work report gelatin liquefaction or not. The type or shape of liquefaction is of less importance.

Oxoid OGYE Selective Supplement (Oxytetracycline Glucose Yeast Extract) is added to Oxytetracycline Glucose Yeast Extract Agar Base, Part No. CM0545B.

• Add to Oxytetracycline Glucose Yeast Extract Agar Base, Part No. CM0545B
• Each vial supplements 500mL of medium

OGYE Selective Supplement, Oxoid Composition

OGYE Selective Medium Preparation:
Suspend 18.5g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 10 minutes. Allow the medium to cool to 50°C and aseptically add the contents of one vial of Oxytetracycline GYE Selective Supplement (SR0073) reconstituted as directed. Mix thoroughly and pour into sterile Petri dishes. The pH of this medium should be 7.0±0.2 @ 25°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product has been discontinued, please refer to SR0258E

Thermo Scientific™ Oxoid OCLA (ISO) Differential Supplement is a differential supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Differential Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Thermo Scientific™ Oxoid OCLA (ISO) Selective Supplement is a selective supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Selective Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between E. coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies.

ONE Broth-Listeria Base, Oxoid Composition

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Base must be stored tightly capped in the original container at 10-30°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

Precautions
ONE Broth-Listeria Base and ONE Broth Selective Supplement are for Laboratory Use Only.
ONE Broth-Listeria Base is designated as “Irritant” and ONE Broth-Listeria Selective Supplement is designated as “Harmful”. For a full Material Safety Data Sheet (MSDS).
Do not use ONE Broth-Listeria Base or ONE Broth-Listeria Selective Supplement beyond the stated expiry dates, or if the products show any sign of deterioration.

Thermo Scientific™ Oxoid ONE Broth Salmonella Selective Supplement is a selective supplement for use with ONE Broth-Salmonella Base.

ONE Broth Salmonella Selective Supplement, Oxoid Composition

ONE Broth Salmonella Selective Medium Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
The ONE Broth-Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

The Oxoid ONE Broth-Listeria is for the selective enrichment broth for Listeria species from food samples in 24 hours.

One Broth-Listeria Selective Supplement
ONE Broth-Listeria is the selective enrichment broth used in the Oxoid Listeria Precis™ rapid culture method. For information simply download the ONE Broth-Listeria data sheet (273KB) or the Listeria Precis data sheet (680KB) in PDF format.

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Selective Supplement should be stored in the dark at 2-8°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

Enrich and recover Salmonella spp. from food samples in a single step with Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Base, when prepared with Thermo Scientific Oxoid ONE Broth-Salmonella Selective Supplement (SR0242).

ONE Broth-Salmonella Base, Oxoid Composition

ONE Broth-Salmonella Base, Oxoid Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
ONE Broth-Salmonella should be stored tightly capped in the original container at 10-30ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
ONE Broth-Salmonella medium and Brilliance Salmonella Agar are for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration. Sterilize specimens, equipment and media properly after use; dispose waste in accordance with local rules and regulations. Directions should be read and followed carefully.

Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Supplement is for the selective enrichment of Salmonella spp. from food samples in 24 hours in a single enrichment step.

• Add to ONE Broth-Salmonella Part No. CM1091B
• Each vial supplements 500mL of medium

ONE Broth Salmonella Selective Supplement, Oxoid Composition

ONE Broth Salmonella Selective Medium Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
The ONE Broth-Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Isolate and enumerate spoilage organisms of citrus products with Thermo Scientific™ Oxoid™ Orange Serum Agar (Dehydrated). Orange Serum Agar is developed specifically for the isolation and enumeration of microorganisms that are capable of surviving citrus products. The low pH of these products limits the growth of microorganisms to those capable of tolerating the acidic environment.

Orange Serum Agar, Oxoid Composition

Orange Serum Agar, Oxoid Preparation:
Suspend 37g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

Oxoid ORSAB Selective Supplement (Oxacillin Resistance Screening Agar Base Selective Supplement) is added to Oxacillin Resistance Screening Agar Base, Part No. CM1008B.

• Add to Oxacillin Resistance Screening Agar Base, Part No. CM1008B
• Each vial supplements 500mL of medium

ORSAB Selective Supplement, Oxoid Composition

ORSAB Selective Medium Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
ORSAB Selective Supplement should be stored at 2-8°C.
Store the prepared medium at 2-8°C.