Microbiology

Oxoid Edwards Medium, Modified is used for selective isolation of Streptococcus agalactiae.

Edwards Medium, Modified, Oxoid Composition

Edwards Medium, Modified, Oxoid Preparation:
Suspend 41g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 50°C, add 5-7% of sterile bovine or sheep blood, mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C. and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

This product has been discontinued. The alternative product is Thioglycollate Fluid Medium USP (CM0173).

Oxoid Thioglycollate Medium, Brewer is an anaerobic medium especially useful for sterility control of solutions containing mercury-based preservatives.

Thioglycollate Medium, Brewer, Oxoid Composition

Thioglycollate Medium, Brewer, Oxoid Preparation:
Suspend 20g in 1 litre of distilled water. Bring to the boil, mix well and allow to stand until completely dissolved. Distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Media containing small quantities of agar are liable to separate if cooled rapidly. Tubes of reconstituted and autoclaved medium should be allowed to cool slowly on a wooden surface in a draught-free atmosphere.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at room temperature.

Precautions
Check the upper portion of the prepared medium before inoculation. If more than one-third of the fluid is oxidised i.e. green coloured, discard the bottle.

If one-third or less fluid is oxidised, then heat in boiling water with the cap loosened to drive off oxygen. Cool to room temperature before inoculation. This reheating process can only be carried out once because of the formation of toxic radicles in the medium.

Organisms which ferment glucose and lower the pH to critical levels may not survive in this medium after growth has taken place.

Oxoid Milk Agar, Oxoid is for use in the dairy industry.

Milk Agar, Oxoid Composition

Milk Agar, Oxoid Preparation:
Suspend 24g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Carry out plate counts of microorganisms in water with Thermo Scientific™ Oxoid™ Yeast Extract Agar (Dehydrated). Yeast Extract Agar is made to the formula described by Windle Taylor for the plate count of microorganisms in water and is tested in accordance with ISO 1133:2014. It is a rich, nutritious medium for the recovery of a wide range of bacteria, yeast, and molds.

Yeast Extract Agar, Oxoid Composition

Yeast Extract Agar, Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Lab-Lemco Broth is a nutrient medium equivalent to USA nutrient extract formulations.

Lab-Lemco Broth, Oxoid Composition

Lab-Lemco Broth, Oxoid Preparation:
Dissolve 8g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.
`Lab-Lemco’ Broth is slightly more alkaline than the medium used in the APHA publications (pH 6.8).

Peptone water (CM0009B), Oxoid, is a basal medium that used as a growth medium or as the basis of carbohydrate fermentation media. A pure culture in Peptone Water is a convenient inoculum for a series of fermentation tubes or other diagnostic media.

Directions

Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

MacConkey agar, Oxoid is used to detect, isolate and enumerate coliforms and intestinal pathogens in water, dairy products and biological specimens. This differential medium gives a number of diagnostic indications such as bile tolerance, colony morphology and chromogenesis. MacConkey Agar corresponds to the medium recommended by the World Health Organizaton, the Department of Health for the bacterial examination of water.

Composition of MacConkey Agar (Oxoid)

Preparation of MacConkey Agar (Oxoid):

Suspend 52g of MacConkey agar powder in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life of MacConkey Agar (Oxoid)
Store the dehydrated MacConkey agar powder at 10-30°C and use before the expiry date on the label.
Store the prepared MacConkey agar at 2-8°C.

Identify coliforms from water and milk samples with differential Thermo Scientific™ Oxoid™ MacConkey Broth (Dehydrated). MacConkey Broth is the standard medium for the primary isolation of coliform bacteria, and has been recommended for this purpose by the Public Health Laboratory Service Water Committee and the World Health Organization. MacConkey Broth also conforms to the formulation specified by the UK Dept. of Health for milk grading.

MacConkey Broth, Oxoid Composition

MacConkey Broth, Oxoid Preparation:
To prepare single strength broth, add 40g to 1 litre of distilled water. Mix well and distribute into containers fitted with fermentation (Durham) tubes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The neutral red indicator is carefully selected for this formulation and therefore shows no inhibitory effect. However, the more sensitive reaction of bromocresol purple in MacConkey Broth Purple (CM0505) is often preferred.

Oxoid Nutrient Agar Tablets make 5mL of medium each.

Nutrient Agar Tablets, Oxoid Composition

Nutrient Agar, Oxoid Preparation:
Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid MUG Supplement (50mg) Fluorescent agent is used for the detection of E. coli.

• Contains 4-methylumbelliferyl-β-D-glucuronide (MUG)
• Each vial supplements 500mL of medium

M.U.G. Supplement, Oxoid Composition

M.U.G. Supplement, Oxoid Preparation:

Add 2ml of distilled water to a vial and invert gently until completely dissolved. Add the vial contents to the following volumes of suggested media, before sterilisation.

Storage conditions and Shelf life
Should be stored at 2-8°C. When stored as directed the unopened vial is stable until the expiry date on the label.

Precautions
The presence of endogenous glucuronidase in shellfish samples may result in false positive fluorescence. Test tubes used in the MPN method should be checked under UV light to ensure the glass does not fluoresce. To avoid false positive fluorescence the source of long wave UV light must not exceed 6 watts.

This product is temporarily unavailable. Please contact us for more information.

Oxoid Saline Tablets is used for preparation of 500mL of 0.85% isotonic saline. For Laboratory Use Only.

Oxoid Ringers Solution Tablets is used for the preparation of isotonic diluents for bacterial cells and bacteriological specimens.

Each tablet prepares 500mL solution. For Laboratory Use Only.

Ringers Solution Tablets, Oxoid Composition

One tablet makes 500 ml of quarter-strength Ringers solution.

Ringers Solution Preparation:
To prepare quarter-strength Ringers Solution, dissolve 1 tablet in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes.

Oxoid Basic Fuchsin Indicator is a dye for use with Endo Agar Base, Part No. CM0479B and m Endo (LES) Agar Base, Part No. MM0551B.

Basic Fuchsin Preparation:

BR0050 should be dissolved to create a 10% w/v solution using 95% ethyl alcohol.

For each litre of culture medium use the amount described in the product reconstitution details.  This should be in a 10% w/v solution of the dye dissolved in 50:50 ethanol/distilled water.  E.g. For CM0479 add 6ml of a 10% w/v Basic Fuchsin solution per litre of medium.

The magenta dyes are closely related to known carcinogenic substances; therefore basic fuchsin should be handled with care, avoiding inhaling the powder or staining the skin with dye.

• Oxoid Thiosulphate Ringers Tablets is used for preparation of isotonic diluents for bacterial cells and bacteriological specimens.
• Each tablet prepares 500mL solution.

Thiosulphate Ringers Tablets, Oxoid Composition

* Due to the low ionic content of this medium no pH is specified.
One tablet makes 500ml of quarter-strength Ringer containing 0.05% of sodium thiosulphate.

Thiosulphate Ringers Tablets, Oxoid Preparation:
To prepare quarter-strength Ringer Solution containing thiosulphate, dissolve 1 tablet in 500ml distilled water. Sterilise by autoclaving at 121°C for 15 minutes.

Phosphate Buffered Saline Tablets, Dulbecco A (PBS, Oxoid) is used for the preparation of a balanced salt solution without calcium and magnesium. PBS is normally used to rinse the cells free from growth medium before application of the chelating agent, such as Versene (diamino-ethane-tetra-acetic acid).

Content of Phosphate Buffered Saline, Oxoid:

Preparation of PBS Buffer from PBS tablets, Oxoid
Dissolve ONE tablet in 100ml of distilled water. Sterilize the PBS solution with autoclaving for 115°C for 15 mins. The tablets should be dissolving completely and free from insoluble matter.