Microbiology

For the preparation of serum-dextrose-antibiotic medium for the cultivation and isolation of Brucella using Brucella Selective Supplement SR0083 or SR0209. Without antibiotics, it may be used in conjunction with the Cruickshank dyestrip method for differentiation between strains.

Brucella Medium Base, Oxoid Composition

Brucella Medium, Oxoid Preparation:
Suspend 45 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5% of inactivated Horse Serum (i.e. Horse Serum held at 56°C for 30 minutes). Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Isolate, enumerate and differentiate coliforms in dairy products with differential Thermo Scientific™ Oxoid™ Desoxycholate Agar (Dehydrated). The medium is also used for the isolation of enteric pathogens from rectal swabs, feces or other specimens. Differentiation of coliforms is based on the fermentation of lactose. Lactose fermenting bacteria form pink colored colonies while non-lactose fermenters form straw colored colonies.

Desoxycholate Agar, Oxoid Composition

Desoxycholate Agar, Oxoid Preparation:
Suspend 45g in 1 litre of distilled water. Bring to the boil over gauze and flame to dissolve the medium completely. Agitate to prevent charring.
THIS MEDIUM IS HEAT SENSITIVE. AVOID EXCESSIVE OR PROLONGED HEATING DURING RECONSTITUTION. DO NOT AUTOCLAVE OR REMELT.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Oxoid Trichomonas Medium is used for cultivation of Trichomonas vaginalis.

Trichomonas Medium, Oxoid Composition

Trichomonas Medium, Oxoid Preparation:
Suspend 37.5g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Inactivate 80ml of horse serum, adjust to pH 6.0 and add it to the medium. For diagnostic work, bacterial growth may be suppressed by the addition of 1000 units of penicillin and 500µg of streptomycin per ml of medium or 100µg of chloramphenicol per ml of medium (1 vial of SR0078 per 500ml medium).

Prior to incorporation in the medium, Horse Serum SR0035 is inactivated by maintaining at a temperature of 56°C for 30 minutes, and then acidified with 1N hydrochloric acid to pH 6.0.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Simmons Citrate Agar (Dehydrated), Oxoid Composition

Simmons Citrate Agar (Dehydrated), Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is important not to carry over any nutrients into the citrate medium because this will result in false positive tests. Dilute the inoculum in saline before inoculating the citrate medium to avoid a carry-over of other carbon sources.

Cultivate and enumerate anaerobes, especially Clostridium species, with Thermo Scientific™ Oxoid™ Reinforced Clostridial Agar (RC Agar) (Dehydrated). A solid, highly nutritious medium, it can be used as an enrichment medium to optimize the growth of clostridia and other anaerobes, lactobacilli, and many other species of bacteria.

Reinforced Clostridial Agar (RC Agar) (Dehydrated), Oxoid Composition

Reinforced Clostridial Agar (RC Agar) (Dehydrated), Oxoid Preparation:
Suspend 52.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Further identification tests must be carried out on organisms isolated from this medium.

Cultivate and enumerate clostridia and other anaerobes occurring in food and pathological samples with Thermo Scientific™ Oxoid™ Reinforced Clostridial Medium. A semi-solid, highly nutritious medium can be used as an enrichment medium to optimize the growth of clostridia before sub-culturing to solid agar.

Reinforced Clostridial Medium, Oxoid Composition

Reinforced Clostridial Medium, Oxoid Preparation:
Suspend 38 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Sabouraud Liquid Medium is an USP formulation for mycological sterility testing.

Sabouraud Liquid Medium, Oxoid Composition

Sabouraud Liquid Medium, Oxoid Preparation:
Dissolve 30g in 1 litre of water (purified as required). Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away from light between 2-25°C.

Oxoid Staphylococcus Medium No.110 is a selective medium is used for isolation and differentiation of pathogenic Staphylococci.

Staphylococcus Medium No.110, Oxoid Composition

Staphylococcus Medium No.110, Oxoid Preparation:
Suspend 150g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Disperse the precipitate by gentle agitation before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Enterococcus faecalis may grow on this medium as tiny colonies with slight mannitol fermentation.
The high salt content in Staphylococcus Medium No.110 may interfere with the coagulase reaction. Always sub-culture to a non-inhibitory medium before testing.

Isolate and enumerate yeasts and molds from dairy and food products and prepare Aspergillus niger for the Harmonised Microbial Limit Tests from international pharmacopoeia with Thermo Scientific™ Oxoid™ Potato Dextrose Agar (Dehydrated). This medium is suitable for the detection and enumeration of heat resistant molds in thermally processed fruits and fruit products.

Pigment production is a critical part of the identification of the fungus. The agar used in the medium is carefully screened to ensure correct pigment production by fungi such as Fusaria spp.

Composition of Potato Dextrose Agar

Preparation of Potato Dextrose Agar, Oxoid
Suspend 39g in 1 litre of purified water and heat up to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Ensure the solution is well mixed before pouring.

To suppress the growth of bacteria, you can acidify the medium to pH 3.5 by adding 1 ml of lactic acid 10% SR0021 to each sterilized medium at 50°C. Note that the medium must not be heated after the addition of acid as it will destroy the gelling properties and results in hydrolysis of agar.

Storage Conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Presumptively identify coliform organisms in milk, water and foods as specified by the American Public Health Association with Thermo Scientific™ Oxoid™ Lactose Broth (Dehydrated). Beef extracts and peptone in this liquid medium supply essential nutrients while lactose is a fermentable carbohydrate for coliforms. Coliforms ferment lactose in the medium resulting in gas formation which is indicative of positive results.

Lactose Broth, Oxoid Composition

Lactose Broth, Oxoid Preparation:
Dissolve 13g in 1 litre of distilled water and distribute into containers with fermentation tubes (Durham). Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature (18-22°C).

Precautions
Ensure that the fermentation tubes are free from air bubbles before inoculation.
Large water samples may require double-strength lactose broth to reduce the final volumes. Do not overheat double- strength broth or inhibitory products will be produced.

Grow a wide variety of microorganisms with Thermo Scientific ™ Oxoid ™ Tryptone Soya Agar EP/USP/JP/BP (Dehydrated). A general purpose agar medium containing two peptones, supports the growth of both aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. Tryptone Soya Agar is recommended as a reference medium when testing selective media to measure the degree of inhibition.

*Contains papain

Preparation of Tryptone Soya Agar, Oxoid

First, you add 40g of tryptone soya agar to 1 litre of distilled water (purified as required). You can boil to dissolve the powder completely. After dissolved, you can sterilise the medium by autoclaving at 121°C for 15 minutes.

Storage Condition and Shelf Life of Tryptone Soya Agar, Oxoid

You can store the dehydrated tryptone soya agar at 10-30°C and use before the expiry date on the label or store the prepared plates of a medium at 2-8°C.

Tryptone Soya Agar, Oxoid (dehydrated) is suitable to grow a wide variety of microorganisms. It’s a general-purpose agar medium containing two peptones, which supports the growth of both aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. Tryptone Soya Agar is recommended as a reference medium when testing selective media to measure the degree of inhibition.

*Contains papain

Preparation of Tryptone Soya Agar, Oxoid

First, you add 40g of tryptone soya agar to 1 litre of distilled water (purified as required). You can boil to dissolve the powder completely. After dissolved, you can sterilise the medium by autoclaving at 121°C for 15 minutes.

Storage Condition and Shelf Life of Tryptone Soya Agar, Oxoid

You can store the dehydrated tryptone soya agar at 10-30°C and use before the expiry date on the label or store the prepared plates of a medium at 2-8°C.

Thermo Scientific™ Oxoid Tryptone Soya Broth (Tryptic Soy Broth) (Soybean Casein Digest Medium USP) is used for general laboratory use and sterility testing.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Grow a wide variety of microorganisms with Thermo Scientific™ Oxoid™ Tryptone Soya Broth (a.k.a Tryptic Soy Broth) EP/USP/BP/JP (Dehydrated). The general purpose medium, containing two peptones, is suitable for the cultivation of both aerobes and anaerobes. It is specified in International Pharmacopoeia and food testing methods for the growth of a wide variety of organisms.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Oxoid Tryptone Glucose Extract Agar is a standard method medium for examination of water and dairy products.

Tryptone Glucose Extract Agar, Oxoid Composition

Tryptone Glucose Extract Agar, Oxoid Preparation:
Suspend 24 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. When the dilution of the original specimen is greater than 1 in 10, add 10 ml of sterile 10% solution of Skim Milk Powder LP0031 per litre.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Charcoal Agar Base (Bordetella Selective Medium) is used for selective isolation of B. pertussis and B. parapertussis.
• Requires addition of Bordetella Selective Supplement, Part No. SR0082E.

Charcoal Agar Base, Oxoid Composition

Charcoal Agar, Oxoid Preparation:
Suspend 51g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, add 10% of defibrinated blood and mix gently. The medium is made selective for the isolation of Bordetella pertussis and Bordetella parapertussis by the addition of Bordetella Selective Supplement SR0082.

To one vial add 2ml of sterile distilled water and dissolve the contents completely. Add this solution to 500ml of sterile, molten Charcoal Agar, cooled to 50°C, together with 10% v/v defibrinated horse blood SR0050. Mix well before pouring into sterile Petri dishes.
For Haemophilus influenzae, omit the selective agents and convert to `chocolate’ agar.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Stuart’s transport medium or similar formulation media should not be used for Bordetella-containing specimens.
Two pernasal swabs should be taken from each patient, one through each nostril.
Make sure the charcoal remains in suspension when dispensing the medium by gently swirling the flask.
Lysed horse blood is used in the transport medium but whole blood is used in the isolation medium.
Most naso-pharyngeal flora are inhibited by cephalexin but Pseudomonas aeruginosa and some fungi may grow through. Amphotericin B can be added (12mg/ml) as an antifungal agent to prevent the growth of filamentous fungi. However, this level of amphotericin B can be inhibitory to Bordetella pertussis and should not be used routinely.

Detect and enumerate coliform organisms, including Escherichia coli, while isolating and detecting Salmonella and Shigella spp. in both clinical and food specimens using Thermo Scientific™ Oxoid™ MacConkey Agar No.3. A modification of the original MacConkey medium by the addition of selected bile salts, the medium gives improved differentiation of coliforms and non-lactose fermenting organisms while completely inhibiting Gram-positive cocci.

MacConkey Agar No.3, Oxoid Composition

MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium at 2-8°C.

**This product is not available at the moment. Please contact us for more information.

Oxoid Tomato Juice Agar is used for cultivation of lactobacilli.

Tomato Juice Agar, Oxoid Composition

Tomato Juice Agar, Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the plates of prepared medium at 2-8°C.

Oxoid Stuart Transport Medium is a semi-solid non-nutritional transport medium for fastidious, pathogenic organisms.

Stuart Transport Medium, Oxoid Composition

Stuart Transport Medium, Oxoid Preparation:
Suspend 16g in 1 litre of distilled water. Bring to the boil to dissolve completely and dispense into screw-capped 7ml bottles. Fill each bottle to the brim, tighten the cap and sterilise by autoclaving at 121°C for 15 minutes. When sufficiently cool to handle, mix by inversion.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 15-25°C.

Precautions
A small amount of blue colour at the top of the bottle indicates oxidation. If this colour extends down into the medium it should be discarded.
Avoid prolonged heating in open flasks, during the preparation of the medium, because thioglycollate is volatile.
Sodium glycerophosphate may be metabolised by some organisms and thus promote their growth.

Oxoid MacConkey Agar No. 2 is a modification of MacConkey Agar No. 3 with addition of Bile Salts No. 2 for detection of Enterococci.

MacConkey Agar No. 2, Oxoid Composition

MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Detect and enumerate coliform organisms in water, food and dairy products with Thermo Scientific™ Oxoid™ Violet Red Bile Agar (Dehydrated). This lactose containing selective medium has been used for the determination of the coli-aerogenes content of water, milk and other dairy products, dairy equipment, and food products. Due to the addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Agar (Dehydrated), Oxoid Composition

Violet Red Bile Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms e.g. Aeromonas and Yersinia species may give similar reactions.
The selectivity of the medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth.

Oxoid Corn Meal Agar Mycological medium is used for identification of Candida albicans and maintenance of fungal stock cultures.

Corn Meal Agar, Oxoid Composition

Corn Meal Agar, Oxoid Preparation:
Suspend 17g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Glucose supplemented Corn Meal Agar should not be used for chlamydospore production.
Corn Meal Agar with `Tween 80’ (or other wetting agents) will allow Candida stellatoides and Candida tropicalis to produce chlamydospores.
Some Candida strains lose their ability to produce chlamydospores after repeated subculturing.

Isolate and identify Salmonella and Shigella species from clinical or food samples with Thermo Scientific™ Oxoid™ SS Agar (Dehydrated). Differentiation of Salmonella and Shigella is achieved by incorporation of lactose in the medium. Organisms which ferment lactose produce acid which, in the presence of the neutral red indicator, results in the formation of red colonies. Salmonella, Shigella, and other non-lactose-fermenting organisms appear as transparent or straw coloured colonies on SS Agar.

SS Agar, Oxoid Composition

SS Agar, Oxoid Preparation:
Suspend 63g in 1 litre of distilled water. Bring to the boil with frequent agitation and allow to simmer gently to dissolve the agar. DO NOT AUTOCLAVE. Cool to about 50°C, mix and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
This medium is highly selective and R-strains of shigellae will not grow on it. It is not recommended for the primary isolation of shigellae.

Oxoid Czapek Dox Agar Modified is used for cultivation of fungi.

Czapek Dox Agar Modified, Oxoid Composition

Czapek Dox Agar Modified, Oxoid Preparation:
Suspend 45.4g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. If required, adjust the reaction to pH 3.5 ± 0.2 by adding 10ml of Lactic Acid 10% (SR0021) per litre after sterilisation.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.