Microbiology

Thermo Scientific™ Oxoid Vegetable Peptone No. 1 is a highly nutritious, meat-free general purpose peptone for growth of bacteria and fungi.

Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Supplement is for the selective enrichment of Salmonella spp. from food samples in 24 hours in a single enrichment step.

• Add to ONE Broth-Salmonella Part No. CM1091B
• Each vial supplements 500mL of medium

ONE Broth Salmonella Selective Supplement, Oxoid Composition

ONE Broth Salmonella Selective Medium Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
The ONE Broth-Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

The Oxoid ONE Broth-Listeria is for the selective enrichment broth for Listeria species from food samples in 24 hours.

One Broth-Listeria Selective Supplement
ONE Broth-Listeria is the selective enrichment broth used in the Oxoid Listeria Precis™ rapid culture method. For information simply download the ONE Broth-Listeria data sheet (273KB) or the Listeria Precis data sheet (680KB) in PDF format.

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Selective Supplement should be stored in the dark at 2-8°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

Thermo Scientific™ Remel™ Sputasol is a liquifying agent for sputum samples.

• Each vial dilutes in sterile distilled water to make 100mL

Sputasol, Oxoid Composition

Sputasol, Oxoid Preparation:
Aseptically add the contents of one vial (7.5ml) to 92.5ml of sterile distilled water. Use the working solution immediately or store at 2-8°C for upto 48 hours only.

Oxoid Campylobacter Growth Supplement is a liquid supplement for the enhanced growth and aerotolerance of Campylobacter species.

• For the preparation of Blaser-Wang Medium, Bolton Selective Enrichment Broth, Butzler Selective Medium, and Preston Selective Medium
• Each vial supplements 500mL of medium

Campylobacter Growth Supplement, Oxoid Composition

Campylobacter Growth Medium, Oxoid Preparation:
Add the contents of one vial (2 ml) to 500 ml of a sterile nutrient medium cooled to 50-55°C prepared from Oxoid Columbia Agar, Blood Agar Base No.2, or Campylobacter Agar Base, with 5-7% lysed defibrinated horse or sheep blood, and the rehydrated contents of one vial of Campylobacter Antibiotic Supplement SR0069, SR0085 or SR0098. Mix gently and pour aseptically into sterile Petri dishes.

Brilliance Candida is a chromogenic medium for the isolation and differentiation of Candida species.

• Add to to Brilliance Candida Agar Base, Part No. CM1002A and CM1002B
• Each vial supplements 500mL of medium

Brilliance Candida Selective Supplement, Oxoid Composition 

Brilliance Candida Agar, Oxoid Preparation:
Suspend 15.6g in 500ml of distilled water. Add 1 vial of Brilliance Candida Selective Supplement reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool the medium to 45°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Brilliance Candida Selective Supplement must be stored at 2-8°C. When stored as directed the unopened products will remain stable until the expiry date printed on the containers.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

• Brilliance is a chromogenic medium for the isolation and differentiation of Bacillus cereus in food samples.
• Add to Brilliance Bacillus Cereus Agar Base, Part No. CM1036A and CM1036B. Each vial supplements 500mL of medium.

Brilliance Bacillus Cereus Selective Supplement, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0258E

Oxoid Brilliance Listeria Differential Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria

• Add to Brilliance Listeria Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Oxoid Brilliance Listeria Differential Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Brilliance Listeria Selective Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria spp. in food samples.

• Add to Brilliance Listeria Selective Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Brilliance Listeria Selective Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Cycloheximide Solution (0.1%) (100mg/vial) is used for the suppression of fungi.
• Add to Bacillus Cereus Selective Agar Base, Part No. CM0617B; Raka-Ray Agar Base, Part No. CM0777B; and Universal Beer Agar, Part No. CM0651B.

Oxoid Modified Brucella Selective Supplement is for the cultivation and isolation of Brucella spp.

• Alternative supplement containing natamycin as a replacement for cycloheximide
• Add to Blood Agar Base No. 2, Part No. CM0271B or Brucella Medium Base, Part No. CM0169B
• Each vial supplements 500mL of medium

Modified Brucella Selective Supplement, Oxoid Composition

Modified Brucella Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml Brucella Medium Base, Columbia Agar Base or Blood Agar Base No.2 together with 5-10% v/v inactivated horse serum SR0035 and 1-5% w/v sterile solution of glucose, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Oxoid Modified Bolton Broth Selective Supplement is for the selective pre-enrichment of Campylobacter spp. in food samples.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Bolton Broth, Part No. CM0983B
• Each vial supplements 500mL of medium

Modified Bolton Broth Selective Supplement, Oxoid Composition

Modified Bolton Broth Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Bolton Broth CM0983 cooled to approximately 50°C with 25 ml of laked horse blood SR0048. Mix well and distribute into sterile containers.

• Oxoid Listeria Selective Supplement (Oxford) is used for the detection of Listeria monocytogenes.
• Add to Listeria Selective Agar Base, Part No. CM0856B.

Modified Listeria Selective Supplement (Oxford), Oxoid Composition

Modified Listeria Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to500 ml of sterilised Listeria Selective Agar Base (Oxford Formulation) CM0856, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C in the dark.

Oxoid Modified Karmali Selective Supplement is for a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42° C.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Campylobacter Agar Base (Karmali), Part No. CM0935B
• Each vial supplements 500mL of medium

Modified Karmali Selective Supplement, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Campylobacter Agar Base (Karmali) CM0935 cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Oxoid Modified Preston Campylobacter Selective Supplement is for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian, and environmental samples.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Campylobacter Agar Base, Part No. CM0689B or Nutrient Broth No. 2, Part No. CM0067B
• Each vial supplements 500mL of medium

Modified Preston Campylobacter Selective Supplement, Oxoid Composition

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Salmonella Selective Supplement is used for the presumptive identification of Salmonella species from clinical and food samples.

• Add to Chromogenic Salmonella Agar Base, Part No. CM1007B or Brilliance™ Salmonella Agar Base, Part No. CM1092B
• Each vial supplements 500mL of medium

Salmonella Selective Supplement, Oxoid Composition

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Oxoid Cefixime Supplement is used for the isolation of Escherichia coli O157.

• Add to to Cefixime Rhamnose Sorbitol MacConkey Agar Base, Part No. CM1005B
• Each vial supplements 500mL of medium

Cefixime Supplement, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

Oxoid Burkholderia Cepacia Selective Supplement is used for the isolation of Burkholderia cepacia.

• Add to Burkholderia Cepacia Agar Base, Part No. CM0995B
• Each vial supplements 500mL of medium

Burkholderia Cepacia Selective Supplement, Oxoid Composition

Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.

• Each vial supplements 500mL of medium
• Format: Freeze dried

Vancomycin Supplement, Oxoid Composition

Storage conditions and Shelf life
Vancomycin Supplement SR0186 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Oxoid Gentamicin Selective Supplement (256mg/vial) is used for the isolation of Vancomycin-Resistant Enterococci (VRE) and High Level Aminoglycoside-Resistant Enterococci (HLARE) from clinical specimens.

• Add to VRE Agar Base, Part No. CM0985B
• Each vial supplements 500mL of medium

Gentamicin Selective Supplement, Oxoid Composition

Storage conditions and Shelf life
Gentamicin Supplement SR0185 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.