Microbiology

Oxoid RPF Supplement (Rabbit Plasma Fibrinogen) is used for the isolation and enumeration of coagulase positive staphylococci from food.

• Use only with Baird-Parker Agar Base, RPF, Part No. CM0961B
• Each vial supplements 100mL of medium

RPF Supplement (Rabbit Plasma Fibrinogen), Oxoid Composition

RPF Supplement Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90 ml of sterile Baird-Parker Agar Base (RPF) CM0961 cooled to 48°C. Mix well and use immediately.

Precautions
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

This product has been discontinued. The recommended alternative is Modified Preston Campylobacter Supplement, SR0204E.

Oxoid Preston Campylobacter Selective Supplement is used for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian, and environmental samples.

• Add to Campylobacter Agar Base, Part No. CM0689B or Nutrient Broth No. 2, Part No. CM0067B
• Each vial supplements 500mL of medium

Preston Campylobacter Selective Supplement, Oxoid Composition

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thermo Scientific™ Oxoid Nitrocefin is used for detection of beta-lactamase production.

• Reconstitutes to 500μg/mL
• Contents: 5 vials lyophilized nitrocefin (1mg) and 5 vials rehydrating fluid (1.9mL)

• Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
• Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

• Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
• Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Oxoid Yersinia Selective Supplement is used for the isolation of Yersinia enterocolitica.

• Add to Yersinia Selective Agar Base, Part No. CM0653B
• Each vial supplements 500mL of medium

Yersinia Selective Supplement, Oxoid Composition

Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.

Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.

This product is not available at the moment. Please contact us for more information.

Oxoid G-N Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

G-N Anaerobe Selective Supplement, Oxoid Composition

Selective Medium for Gram-Negative Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of G-N Anaerobe Supplement (SR0108) rehydrated as directed, together with 25ml defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

This product is not available at the moment. Please contact us for more information.

Oxoid N-S Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

N-S Anaerobe Selective Supplement , Oxoid Composition

Selective Medium for Non-Sporing Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water containing 0.5ml `Tween 80’. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of N-S Anaerobe Supplement (SR0107) rehydrated as directed, together with 25ml of defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

Oxoid Yeast Autolysate Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

Yeast Autolysate Supplement, Oxoid Composition

Yeast Autolysate Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053. Mix gently and pour into sterile Petri dishes.

Oxoid C-F-C Supplement is used for the isolation of Pseudomonas species.

• Add to to Pseudomonas Agar Base, Part No. CM0559B
• Each vial supplements 500mL of medium

Pseudomonas CFC Supplement, Oxoid Composition

Pseudomonas CFC Agar, Oxoid Preparation:
To 500ml of agar base cooled to 50°C add the contents of 1 vial of Pseudomonas CFC Supplement (SR0103) rehydrated as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Fresh media should be prepared as required. Molten agar should not be kept longer than 4 hours. Medium should not be stored and remelted. If swarming colonies of Proteus species are a problem in food samples then the incubation temperature can be lowered to 20°C for a period of 3-5 days. Chilled foods may carry a wide range of pseudomonads and the colonies on CFC Medium, incubated at lower temperatures, may be Pseudomonas fluorescens or Pseudomonas putida as well as Pseudomonas aeruginosa. Aeromonas species will also appear as pink/brown colonies, particularly from fish products.

Oxoid C-N Supplement is used for the isolation of Pseudomonas aeruginosa.

• Add to to Pseudomonas Agar Base, Part No. CM0559B
• Each vial supplements 500mL of medium

Pseudomonas CN Supplement, Oxoid Composition

Pseudomonas CN Agar, Oxoid Preparation:
To 500ml of agar base cooled to 50°C add the contents of 1 vial of Pseudomonas CN Supplement (SR0102) rehydrated as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Fresh media should be prepared as required. Molten agar should not be kept longer than 4 hours. Medium should not be stored and remelted. If swarming colonies of Proteus species are a problem in food samples then the incubation temperature can be lowered to 20°C for a period of 3-5 days. Chilled foods may carry a wide range of pseudomonads and the colonies on CFC Medium, incubated at lower temperatures, may be Pseudomonas fluorescens or Pseudomonas putida as well as Pseudomonas aeruginosa. Aeromonas species will also appear as pink/brown colonies, particularly from fish products.

This product has been discontinued. Please contact us for more information.

Oxoid VCN Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

VCN Selective Supplement, Oxoid Composition

VCN Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Thayer Martin Medium, cooled to 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 plus the rehydrated contents of one vial of Vitox SR0090. Mix gently and pour into sterile Petri dishes.

Oxoid Bacillus Cereus Selective Supplement (Polymyxin B Supplement) is supplied 50,000 IU/Vial for inhibition of Gram-negative bacteria.

• May be added to Bacillus cereus Selective Agar Base, Part No. CM0617B and MYP Agar Base, Part No. CM0929B
• Each vial supplements 500mL of medium

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Composition

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Preparation:
Reconstitute as directed in the product instructions for use. Aseptically, add one vial to 500ml of liquid or molten base medium, which is below 50°C. Mix well, and dispense into appropriate, sterile containers.

Storage conditions and Shelf life
Polymixin B Supplement at 2-8°C and use before the expiry date on the label.

Oxoid Campylobacter Selective Supplement, Blaser-Wang is used to prepare of Blaser-Wang Selective Medium for the isolation of Campylobacter while inhibiting growth of Candida albicans.

• Add to Columbia Blood Agar Base, Part No. CM0271B
• Each vial supplements 500mL of medium

Campylobacter Selective Supplement, Blaser-Wang, Oxoid Composition

Campylobacter Selective Medium, Oxoid Preparation:
Reconstitue one vial as directed add the contents of one vial to 500ml of sterile nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2, with 10% defibrinated horse/sheep blood or 5-7% laked horse blood SR0050, SR0051 or SR0048. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Clostridium Difficile Selective Supplement is added to Clostridium Difficile Agar Base, Part No. CM0601B.
• Each vial supplements 500mL of medium.

Clostridium Difficile Selective Supplement, Oxoid Composition

Clostridium Difficile Agar, Oxoid Preparation:
Suspend 34.5g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and add aseptically the contents of 1 vial of Oxoid Clostridium Difficile Supplement (SR0096) reconstituted as directed, together with 7% (v/v) Defibrinated Horse Blood (SR0050). Sheep Blood (SR0051) may be used in place of Horse Blood but some strains of the organism will show a slightly reduced growth recovery. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C no longer than 5-7 days.

Oxoid LCAT Selective Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

LCAT Selective Supplement, Oxoid Composition

LCAT Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 490ml of sterile GC Agar Base CM0367, cooled to approximately 50°C, containing 50 ml Laked Horse Blood SR0048 and the rehydrated contents of one vial of Yeast Autolysate Supplement SR0105. Mix gently and pour into sterile Petri dishes.

Oxoid Perfringens SFP Supplement is for the selective isolation of Clostridium perfringens.

• Add to Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens SFP Supplement, Oxoid Composition

Shahidi-Ferguson Perfringens Agar (SFP Agar) Preparation:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Kanamycin Sulphate Supplement (10mg/vial) is added to Kanamycin Esculin Azide Agar Base, Part No. CM0591B.

Kanamycin Sulphate Supplement, Oxoid Composition

Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Add 2ml of sterile distilled water to one vial and mix gently to dissolve completely. Add the contents to 500ml of Kanamycin Aesculin Azide Agar Base.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.

Oxoid VCNT Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B

VCNT Selective Supplement, Oxoid Composition

VCNT Selective Medium Preparation:
Aseptically add 2ml of sterile distilled water to one vial mix gently to dissolve the powder completely. Aseptically add the contents of one vial to 500ml of sterile Thayer Martin Medium, cooled to approximately 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 and the rehydrated contents of one vial of Vitox SR0090.

Oxoid Vitox Supplement is used for the preparation of Thayer Martin Medium and New York City Medium for the isolation of Neisseria spp.

• Contents: Vitox Supplement (5 vials) plus reconstitution fluid (5 vials)
• Add to GC Agar Base, Part No. CM0367B

Ordering Information:

• Each vial of Vitox plus reconstitution fluid supplements 500mL of medium

Includes:

• Five vials of Vitox plus five vials of reconstitution fluid

Vitox Supplement, Oxoid Composition

Vitox Rehydration Fluid , Oxoid Composition

Vitox, Oxoid Preparation:
Aseptically add one vial of Hydration Fluid to one vial of Vitox. Mix gently to dissolve completely. Aseptically add the contents of one vial to sterile medium of choice, cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid Vitox Supplement is used for the preparation of Thayer Martin Medium and New York City Medium for the isolation of Neisseria spp.

• Contents: Vitox Supplement (5 vials) plus reconstitution fluid (5 vials)
• Add to GC Agar Base, Part No. CM0367B

Ordering Information:

• Each vial of Vitox plus reconstitution fluid supplements 500mL of medium

Includes:

• Five vials of Vitox plus five vials of reconstitution fluid

Vitox Supplement, Oxoid Composition

Vitox Rehydration Fluid , Oxoid Composition

Vitox, Oxoid Preparation:
Aseptically add one vial of Hydration Fluid to one vial of Vitox. Mix gently to dissolve completely. Aseptically add the contents of one vial to sterile medium of choice, cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid Perfringens TSC Supplement (D-cycloserine 200mg/vial) is used for the recovery of Clostridium perfringens.

• Add to DCLS Agar, Part No. CM0393B, or Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens TSC Supplement, Oxoid Composition

Agar Preparation:
To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.

To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Bordetella Selective Supplement (Cephalexin, 20mg/vial) is used for the selective isolation of Bordetella pertussis and B. parapertussis.
• Add to Charcoal Agar Base, Part No. CM0119B. Each vial supplements 500mL of medium.

Bordetella Selective Supplement, Oxoid Composition

Bordetella Selective Medium, Oxoid Preparation:
Aseptically add 2ml of sterile distilled water to one vial and mix gently to dissolve the contents completely. Aseptically add the contents to 500ml of sterile molten Charcoal Agar CM0119, cooled to approximately 50°C, together with 10% defibrinated horse blood SR0050 . Mix well and pour into sterile Petri dishes.
The vial contents may also be added to half strength Charcoal Agar CM0119 plus 10% defibrinated horse blood SR0050, for use as a transport medium for Bordetella pertussis.

For the isolation of yeast and molds associated with spoilage, add Oxoid Chloramphenicol Selective Supplement to DRBC Agar Base, Part No. CM0727B; for the enumeration of yeast and molds in foods, add to Rose Bengal Chloramphenicol Agar Base, Part No. CM0549B.

Chloramphenicol Selective Supplement, Oxoid Composition

Chloramphenicol Selective Medium, Oxoid Preparation:
Reconstitute one vial SR0078H per 2 litres medium as directed. Add the vial contents to base medium and mix gently. Sterilise by autoclaving (see media for full details), cool to 50°C, mix gently and pour into Petri dishes.