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Oxoid Neomycin Selective Supplement (150mg/vial) allows the growth of clostridia and other anaerobes.

• Add to Blood Agar, Wilkins-Chalgren Anaerobe Agar, Part No. CM0619B, or Columbia Blood Agar, Part No. CM0331B
• Each vial supplements 2L of medium

Neomycin Selective Supplement, Oxoid Composition

Neomycin Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 2 litres of Wilkins Chalgren Agar Base CM0619 or another appropriate medium cooled to approximately 50° C. Mix well and pour into sterile Petri dishes.

Thermo Scientific™ Oxoid Nitrocefin is used for detection of beta-lactamase production.

• Reconstitutes to 500μg/mL
• Contents: 5 vials lyophilized nitrocefin (1mg) and 5 vials rehydrating fluid (1.9mL)

Oxoid Novobiocin Selective Supplement (10mg/vial) is added to EC Broth, Part No. CM0990B; Muller-Kauffmann Tetrathionate-Novobiocin Broth Base, Part No. CM0343B; Brilliant Green Agar, Part No. CM0263B; MRSV, Part No. CM0910B; Modified Tryptone Soya Broth, Part No. CM0989B; and Lysine Iron Agar, Modified, Part No. CM0886B.

• Each vial supplements 500mL of medium

Novobiocin Supplement, Oxoid Composition

Novobiocin Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to the designated medium cooled to approximately 50°C. Mix well and dispense into sterile containers.

Storage conditions and Shelf life
Novobiocin Supplement SR0181 should be stored in the dark at 2-8°C. When stored as directed, the supplement remains stable until the expiry date printed on the label.

Nutrient agar (500g, Oxoid) is a culture media that used to cultivate and isolate fastidious pathogens and other microorganisms. This non-selective medium contains beef extracts combined with peptone, yeast extracts and sodium chloride to provide the nitrogen compounds, carbon, vitamins and some trace ingredients necessary for the growth of bacteria. A general-purpose medium which may be enriched with up to 10% blood or other biological fluid.

Nutrient Agar, Oxoid Composition

Nutrient Agar, Oxoid Preparation:

Powder: Suspend 28g of nutrient agar in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Nutrient Agar Tablets make 5mL of medium each.

Nutrient Agar Tablets, Oxoid Composition

Nutrient Agar, Oxoid Preparation:
Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Cultivate fastidious pathogens and other microorganisms with nutritious Thermo Scientific™ Oxoid™ Nutrient Broth No.2 (Dehydrated). This medium is comparable to a meat infusion and is richer in nutrients than Nutrient Broth (CM0001). It gives good growth from small inocula and is recommended for sterility testing for aerobic organisms. It complies with the recommendations in the ‘British Pharmacopoea’ for the composition of a sterility testing medium for aerobes.

Nutrient Broth No.2, Oxoid Composition

Nutrient Broth No.2, Oxoid Preparation:
Add 25g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Nutrient Gelatin is used for the differentiation of microorganisms by their proteolytic effects.

Nutrient Gelatin, Oxoid Composition

Nutrient Gelatin, Oxoid Preparation:
Suspend 128g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring and cool below 20°C or leave to set in a refrigerator.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not shake the gelatin tubes whilst they are warm because growth and liquefaction of gelatin frequently occurs on the surface layer only.
In routine diagnostic work report gelatin liquefaction or not. The type or shape of liquefaction is of less importance.