Thioglycollate Broth Usp Alternative
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Thermo ScientificTM OxoidTM
Thioglycollate Medium USP – Alternative, is intended for sterility testing with certain biological products that are turbid or otherwise do not lend themselves readily to culturing in Thioglycollate Medium USP (CM0173) because of its viscosity.
Thioglycollate Medium USP – Alternative, Oxoid Composition
| Typical Formula* |
gm/litre |
| L-cystine |
0.5 |
| Sodium chloride |
2.5 |
| Glucose |
5.5 |
| Yeast extract |
5.0 |
| Pancreatic digest of casein |
15.0 |
| Sodium thioglycollate |
0.5 |
| pH 7.1 ± 0.2 @ 25°C |
|
Thioglycollate Medium USP – Alternative, Oxoid Preparation:
Suspend 29g in 1 litre of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilise by autoclaving at 121°C for 15 minutes.
PREPARE FRESHLY OR BOIL AND COOL THE MEDIUM JUST BEFORE USE.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Whilst storage of the prepared medium is not recommended, autoclaved volumes of Thioglycollate Medium USP – Alternative, should be held at 20-30°C in the dark. Storage at lower temperatures increases oxygen absorption.
Precautions
This medium lacks agar and reducing indicator, therefore it is essential that the medium is freshly prepared and used within four hours of preparation.
Thioglycollate media should not be reheated more than once because toxic oxygen radicles are formed on reheating.
Thioglycollate Fluid Medium Usp 500g
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Thermo ScientificTM OxoidTM
Cultivate aerobic and anaerobic organisms in the performance of sterility tests with Thermo Scientific™ Oxoid™ Thioglycollate Medium (USP) (Dehydrated). The medium is prepared according to the formula specified in the US Pharmacopoeia for the performance of sterility tests, and conforms to formulations detailed in the British and European Pharmacopoeia.
Thioglycollate Medium (USP) (Dehydrated), Oxoid Composition
| Formula |
gm/litre |
| Yeast extract |
5.0 |
| Tryptone |
15.0 |
| Glucose |
5.5 |
| Sodium thioglycollate |
0.5 |
| Sodium chloride |
2.5 |
| L-cystine |
0.5 |
| Resazurin |
0.001 |
| Agar |
0.75 |
| pH 7.1 ± 0.2 @ 25°C |
Thioglycollate Medium (USP) (Dehydrated), Oxoid Preparation:
Suspend 29.75 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Distribute into final containers, sterilise by autoclaving at 121°C for 15 minutes. Mix well and cool to room temperature.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away from light at room temperature.
Precautions
If the upper portion of the medium is red because of oxidation, anaerobic conditions can be restored by reheating for 10 minutes in boiling water or steam. Do not reheat more than once.
Following reheating, if more than one-third of the medium is oxidised then it should be discarded.
Some glucose-fermenting organisms which are able to reduce the pH of the medium to a critical level may not survive in this medium. Early subculture is necessary to isolate these organisms.
Thioglycollate Medium (acc. to EP/USP)
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Thioglycollate Medium is tested according to EP/USP.
Thioglycollate Medium, Oxoid Composition
| Formula |
gm/litre |
| Yeast extract |
5.0 |
| Tryptone |
15.0 |
| Glucose |
5.5 |
| Sodium thioglycollate |
0.5 |
| Sodium chloride |
2.5 |
| L-cystine |
0.5 |
| Resazurin |
0.001 |
| Agar |
0.75 |
| pH 7.1 ± 0.2 @ 25°C |
Thioglycollate Medium, Oxoid Preparation:
Suspend Thioglycollate medium (29.75 grams/ 1itre) in de-ionised water. Heat to dissolve. Cool and dispense 100ml into final containers, 100ml DIN bottles. Sterilise at 121°C for 15 minutes. When cooled label each bottle and pack in units of 10 into labelled boxes.
Storage conditions and Shelf life
Store away from light between 2-25ºC. Product shelf life from date of production is 1 year.
Thioglycollate Medium (Brewer) 500g, Oxoid
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Thermo ScientificTM OxoidTM
This product has been discontinued. The alternative product is Thioglycollate Fluid Medium USP (CM0173).
Oxoid Thioglycollate Medium, Brewer is an anaerobic medium especially useful for sterility control of solutions containing mercury-based preservatives.
Thioglycollate Medium, Brewer, Oxoid Composition
| Typical Formula* |
gm/litre |
| `Lab-Lemco’ powder |
1.0 |
| Yeast extract |
2.0 |
| Peptone |
5.0 |
| Glucose |
5.0 |
| Sodium chloride |
5.0 |
| Sodium thioglycollate |
1.1 |
| Methylene blue |
0.002 |
| Agar |
1.0 |
| pH 7.2 ± 0.2 |
Thioglycollate Medium, Brewer, Oxoid Preparation:
Suspend 20g in 1 litre of distilled water. Bring to the boil, mix well and allow to stand until completely dissolved. Distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Media containing small quantities of agar are liable to separate if cooled rapidly. Tubes of reconstituted and autoclaved medium should be allowed to cool slowly on a wooden surface in a draught-free atmosphere.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at room temperature.
Precautions
Check the upper portion of the prepared medium before inoculation. If more than one-third of the fluid is oxidised i.e. green coloured, discard the bottle.
If one-third or less fluid is oxidised, then heat in boiling water with the cap loosened to drive off oxygen. Cool to room temperature before inoculation. This reheating process can only be carried out once because of the formation of toxic radicles in the medium.
Organisms which ferment glucose and lower the pH to critical levels may not survive in this medium after growth has taken place.
Thiosulphate Ringers Tablets
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Thermo ScientificTM OxoidTM
- Oxoid Thiosulphate Ringers Tablets is used for preparation of isotonic diluents for bacterial cells and bacteriological specimens.
- Each tablet prepares 500mL solution.
Thiosulphate Ringers Tablets, Oxoid Composition
| Formula | gm/litre |
| Sodium chloride | 2.15 |
| Potassium chloride | 0.075 |
| Calcium chloride 6H2O | 0.12 |
| Sodium thiosulphate 5H2O | 0.5 |
* Due to the low ionic content of this medium no pH is specified.
One tablet makes 500ml of quarter-strength Ringer containing 0.05% of sodium thiosulphate.
Thiosulphate Ringers Tablets, Oxoid Preparation:
To prepare quarter-strength Ringer Solution containing thiosulphate, dissolve 1 tablet in 500ml distilled water. Sterilise by autoclaving at 121°C for 15 minutes.
Thiourea CP, 500g
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Chemiz
Description
Synonyms: Thiocarbamide
Formula: CH4N2S
Thiourea is an analogue of urea that contains sulphur. Thiourea has been used in protein analysis as ice cold sample buffer. It has the ability increase the solubility and recovery of proteins.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Tic75 Ticarcillin
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Thermo ScientificTM OxoidTM
Tigecyclin 256 MICE (50 pack)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Tigecycline M.I.C.Evaluator Strips (M.I.C.E.™).
Tigecycline 256 MICE (pack of 10)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Tigecycline M.I.C.Evaluator Strips (M.I.C.E.™).
TIL15 Tilmicosin 15µG
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Thermo ScientificTM OxoidTM
Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Tilmicosin Antimicrobial Susceptibility Disks.
Tim85 Ticarcillin/Clavulanic Acid
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Thermo ScientificTM OxoidTM
Tinsdale Medium 500g
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Thermo ScientificTM OxoidTM
Oxoid Tinsdale Agar Base is used for the isolation and identification of Corynebacterium diphtheriae.
Tinsdale Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Proteose peptone |
20.0 |
| Yeast extract |
5.0 |
| Sodium chloride |
5.0 |
| L-cystine |
0.24 |
| Agar |
15.0
|
| pH 7.9 ± 0.2 @ 25°C |
Tinsdale Agar, Oxoid Preparation:
Suspend 9g of agar base in 200ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to approximately 50°C and add the contents of one vial of Oxoid Tinsdale Supplement SR0065 reconstiuted as directed. Mix throughly and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 4 days.
Precautions
Further tests must be carried out on colonies suspected as Corynebacterium diphtheriae, including cell morphology after sub-culture to Loeffler’s Medium and examination for toxin production.
Do not incubate Tinsdale’s Agar plates in enhanced CO2 atmosphere (5-10% v/v).
Tinsdale Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
This product has been discontinued. Please contact us for more information.
Oxoid Tinsdale Supplement is used for the cultivation of Corynebacterium diphtheriae.
- A supplement for Tinsdale Medium, Part No. CM0487B
- Each vial supplements 200mL of medium
Tinsdale Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 200 ml of medium) |
per vial
|
per litre
|
| Serum equiv. |
20.0ml |
100.0ml |
| Potassium tellurite |
0.069g |
0.3445g |
| Sodium thiosulphate |
0.085g |
0.425g |
Tinsdale Agar, Oxoid Preparation:
Suspend 9g of agar base in 200ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to approximately 50°C and add the contents of one vial of Oxoid Tinsdale Supplement SR0065 reconstiuted as directed. Mix throughly and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 4 days.
Precautions
Further tests must be carried out on colonies suspected as Corynebacterium diphtheriae, including cell morphology after sub-culture to Loeffler’s Medium and examination for toxin production.
Do not incubate Tinsdale’s Agar plates in enhanced CO2 atmosphere (5-10% v/v).
TLC Silica gel 60 F₂₅₄ (5×10 cm) Merck
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Merck
Description
For thin-layer chromatography applications, we offer Aluminum TLC plates that are coated with silica gel containing a fluorescent indicator F254. These silica gel TLC plates are available in a set of 50 and measure 5×10 cm. Our classical silica TLC plates are created by combining our established silica gel 60 with a unique polymeric binder. This combination produces a very adherent and robust surface that will not crack or blister and can even withstand writing with a pencil without risking damage to the layer.
TLC Silica gel 60 F₂₅₄, (20×20 cm) – Merck
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Merck
Description
For thin-layer chromatography applications, we offer Aluminum TLC plates that are coated with silica gel containing a fluorescent indicator F254. These silica gel TLC plates are available in a set of 25 and measure 20x20cm. Our classical silica TLC plates are created by combining our established silica gel 60 with a unique polymeric binder. This combination produces a very adherent and robust surface that will not crack or blister and can even withstand writing with a pencil without risking damage to the layer.
TLC Silica gel 60 F₂₅₄, (20×20 cm) – Merck
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Merck
Description
For thin-layer chromatography applications, we offer Aluminum TLC plates that are coated with silica gel containing a fluorescent indicator F254. These silica gel TLC plates are available in a set of 25 and measure 20x20cm. Our classical silica TLC plates are created by combining our established silica gel 60 with a unique polymeric binder. This combination produces a very adherent and robust surface that will not crack or blister and can even withstand writing with a pencil without risking damage to the layer.
Tob10 Tobramycin
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Thermo ScientificTM OxoidTM
Tob30 Tobramycin
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Thermo ScientificTM OxoidTM
Todd-Hewitt Broth 500g
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Thermo ScientificTM OxoidTM
Oxoid Todd-Hewitt Broth is used for cultivation of Streptococci prior to serological grouping.
Todd-Hewitt Broth, Oxoid Composition
| Typical Formula* |
gm/litre |
| Infusion from 450 g fat-free minced meat |
10.0 |
| Tryptone |
20.0 |
| Glucose |
2.0 |
| Sodium bicarbonate |
2.0 |
| Sodium chloride |
2.0 |
| Disodium phosphate |
0.4 |
| pH 7.8 ± 0.2 @ 25°C |
|
Todd-Hewitt Broth, Oxoid Preparation:
Dissolve 36.4 g in 1 litre of distilled water. Mix well, distribute into containers and sterilise by autoclaving at 115°C for 10 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Streptococcus species grown in Todd-Hewitt Broth and harvested as antigens to raise antibodies, will sometimes carry antigenic material from the broth. This problem must be looked for in the antisera. If detected it would be preferable to use another medium to grow the test streptococci.
Toluene for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 108-88-3
Chemical Formula: C₆H₅CH₃
Molar Mass: 92.14 g/mol
Synonyms: Toluol, Methylbenzene
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter.
Tomato Juice Agar 500g
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Thermo ScientificTM OxoidTM
**This product is not available at the moment. Please contact us for more information.
Oxoid Tomato Juice Agar is used for cultivation of lactobacilli.
Tomato Juice Agar, Oxoid Composition
| Typical Formula* |
gm/litre |
| Tomato juice (solids from 400ml) |
20.0 |
| Peptone |
10.0 |
| Peptonised milk |
10.0 |
| Agar |
12.0 |
| pH 6.1 ± 0.2 @ 25°C |
|
Tomato Juice Agar, Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the plates of prepared medium at 2-8°C.
Transfer Pipettes
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Biologix
This product is not available at the moment. Please contact us for more information.
Characteristics
● Polyethylene pipettes will not shatter or break
● An excellent alternative to Pasteur pipettes and rubber bulbs
● Evenly distributed thickness ensures uniform suction force
● Gamma radiation sterilized option available
● Available wrapped individually (sterile) or in bulk package (non-sterile)
TRI Reagent® for processing tissues, cells cultured in monolayer or cell pellets (Sigma-Aldrich)
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Sigma
Description:
Synonyms : DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, TRI Reagent® cell pellet solution, cell pellet RNA extraction, cell pellet RNA isolation, cell pellet RNA purification, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction solution, total RNA isolation solution, total RNA purification solution, TRI Reagent® RNA Isolation Reagent.
TRI reagent® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagent® is a mixture of guanidine thiocyanate and phenol in a monophasic solution.
Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.
Trichloroacetic acid for Analysis EMSURE® ACS,Reag. Ph Eur (Merck)
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Merck
Description
CAS number: 76-03-9
Chemical Formula: CCl₃COOH
Molar Mass: 163.38 g/mol
Product Specification and MSDS for Trichloroacetic acid (Merck).
