Culture Media Products

Selectively enrich Salmonella spp. from food, animal feces, and environmental samples with Thermo Scientific™ Oxoid™ Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO) (Dehydrated). The efficacy of the medium is based upon the ability of Salmonellae to migrate through the selective medium ahead of competing Gram-negative, motile organisms and, thus, produce opaque halos of growth.

Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO), Oxoid Composition

MSRV (ISO) Agar, Oxoid Preparation:
Suspend 31.6g of MSRV (ISO) Agar base in 1 litre of distilled water. Bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool to 50°C, and aseptically add the contents of 1 vial of Novobiocin Supplement (SR0181), reconstituted as directed. Mix well, and pour into sterile Petri dishes. This medium is semi-solid, therefore do not invert plates.

Storage conditions and Shelf life
MSRV (ISO) should be stored in the tightly capped original container at 10-30°C. Novobiocin Supplement should be stored in the dark at 2-8°C. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Precautions
MSRV (ISO) Agar is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.
Basal medium is very hygroscopic.
When handling powder a face mask and gloves must be worn. Refer to material safety data sheet for details.

Perform effortless Antimicrobial Susceptibility Testing (AST) using Thermo Scientific™ Oxoid™ Mueller Hinton Agar (Dehydrated), a medium designed and manufactured according to the European standard, EUCAST and the American standard, CLSI.

Composition of Mueller Hinton Agar, Oxoid

Preparation of Mueller Hinton Agar, Oxoid
Add 38g of Mueller Hinton Agar to 1 litre of distilled water. Bring to the boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life:
Store the dehydrated Mueller Hinton Agar, Oxoid at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton Agar plates at 2-8°C.

Precautions
Incubation in a carbon dioxide enriched atmosphere is not recommended because of its pH effect on the medium. If it is imperative to use CO₂ then known control organisms should be included with the test plates to measure its effect.
Carbohydrates should not be added to Mueller-Hinton Agar because they may influence the rate of growth of the organism and the resulting pH of the medium.
The addition of lysed horse blood to the medium may further reduce the levels of thymidine and prevent the growth of thymidine-dependent organisms.

The medium may not support the growth of some fastidious strains for example microorganisms that require thymine and thymidine for growth. Close adherence to the methodology is required to obtain satisfactory results.

Mueller Hinton Broth, Oxoid conforms to international standards for antimicrobial susceptibility testing media.

Mueller Hinton Broth, Oxoid Preparation
Place 21.0g of Mueller Hinton Broth, Oxoid in 1 litre of distilled water mix to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Chill and adjust cation levels if necessary.

Storage conditions and Shelf life
Store the dehydrated Mueller Hinton broth at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton broth at 2-8°C.

Precautions
Monitor the performance of the broth routinely using the standard QC organisms. If the broth does not yield the expected MIC values, modify the volumes of Mg++ and Ca++ solutions.

If the thymidine content is lowered, after the addition of lysed horse blood or thymidine phosphorylase, the MIC values may be lower.

Oxoid Muller-Kauffmann Tetrathionate Broth Base is selective enrichment medium for isolation of Salmonella spp. and suppression of Proteus species.

Muller-Kauffmann Tetrathionate Broth Base, Oxoid Composition

Muller-Kauffmann Tetrathionate Broth, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil. Cool below 45°C and add, just prior to use, 19ml of iodine solution and 9.5ml of a 0.1% brilliant green solution. Mix well and fill out into sterile tubes or flasks.

Dissolve the potassium iodide in approximately 5ml of distilled water, add the iodide and gently warm the solution to completely dissolve it. Make up the volume to 100ml with distilled water.

Add the brilliant green to the distilled water and shake to dissolve the dye. Heat the solution to 100°C for 30 minutes and shake from time to time whilst cooling, to ensure that the dye has completely dissolved. Store in a brown glass bottle or away from light.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not autoclave the base broth.
Add the iodine solution and brilliant green just prior to use.
The medium is not suitable for the growth of Salmonella typhi, Salmonella sendai, Salmonella pullorum and Salmonella gallinarum.

• Oxoid Mycoplasma Agar Base is used for the selective isolation of Mycoplasma pneumoniae.
• Also available Mycoplasma Supplement P, Part No. SR0060C and Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Agar Base, Oxoid Composition

Mycoplasma Agar, Oxoid Preparation:
Add 35.5g to 1 litre of distilled water. Boil to dissolve the agar and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions in the HAZARDS section.
Do not use thallous acetate media to isolate Ureaplasma urealyticum.
Mycoplasmas may be suspected if (1) typical morphology (2) no growth in media without serum (3) colonies are embedded below the agar surface.

• Oxoid Mycoplasma Broth Base is used for the selective isolation of Mycoplasma species.
• Also available Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Broth Base, Oxoid Composition

Mycoplasma Broth, Oxoid Preparation:
Dissolve 25.5g in 1 litre of distilled water. Mix well and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions stated under Hazards section..
Sub-culture the broth to agar as soon as any indicator added begins to change colour before the pH change destroys the organism.

Oxoid Mycoplasma Supplement G is for the isolation of mycoplasmas.

• Add to Mycoplasma Pneumoniae Agar, Part No. CM0401B or CM0403B
• Each vial supplements 80mL of medium

Mycoplasma Supplement G, Oxoid Composition
POISON – CONTAINS THALLIUM SALT

Mycoplasma Medium Preparation:
Prepare the sterile supplement by aseptically adding 20ml sterile distilled water to the vial and mix gently. Add 1ml of Oxoid Mycoplasma Base without supplements to each of ten small bottles. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set. Aseptically add 2ml of the reconstituted Supplement-P to each bottle containing agar.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Selectively enumerate Bacillus cereus in food samples with Thermo Scientific™ Oxoid™ MYP Agar (Dehydrated). The medium relies upon mannitol fermentation and phospholipase C production to differentiate Bacillus cereus from other members of the species.

Mannitol-negative Bacillus cereus form pink colored colonies due to the presence of Phenol Red Indicator. Egg yolk emulsion helps in the differentiation of phospholipase C producing colonies, which are surrounded by a zone of the white precipitate. Polymixin B Sulphate acts as the inhibitor to restrict the growth of Gram-negative bacteria.

MYP Agar, Oxoid Composition

MYP Agar, Oxoid Preparation:
Suspend 21.5g in 450ml distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes.
Cool to approximately 49°C and aseptically add 50ml Egg Yolk Emulsion SR0047 and 1 vial of Polymyxin B Supplement SR0099, reconstituted as directed. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

This product is not available at the moment. Please contact us for more information.

Oxoid N-S Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

N-S Anaerobe Selective Supplement , Oxoid Composition

Selective Medium for Non-Sporing Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water containing 0.5ml `Tween 80’. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of N-S Anaerobe Supplement (SR0107) rehydrated as directed, together with 25ml of defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

Oxoid Neomycin Selective Supplement (150mg/vial) allows the growth of clostridia and other anaerobes.

• Add to Blood Agar, Wilkins-Chalgren Anaerobe Agar, Part No. CM0619B, or Columbia Blood Agar, Part No. CM0331B
• Each vial supplements 2L of medium

Neomycin Selective Supplement, Oxoid Composition

Neomycin Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 2 litres of Wilkins Chalgren Agar Base CM0619 or another appropriate medium cooled to approximately 50° C. Mix well and pour into sterile Petri dishes.

Thermo Scientific™ Oxoid Nitrocefin is used for detection of beta-lactamase production.

• Reconstitutes to 500μg/mL
• Contents: 5 vials lyophilized nitrocefin (1mg) and 5 vials rehydrating fluid (1.9mL)

Oxoid Novobiocin Selective Supplement (10mg/vial) is added to EC Broth, Part No. CM0990B; Muller-Kauffmann Tetrathionate-Novobiocin Broth Base, Part No. CM0343B; Brilliant Green Agar, Part No. CM0263B; MRSV, Part No. CM0910B; Modified Tryptone Soya Broth, Part No. CM0989B; and Lysine Iron Agar, Modified, Part No. CM0886B.

• Each vial supplements 500mL of medium

Novobiocin Supplement, Oxoid Composition

Novobiocin Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to the designated medium cooled to approximately 50°C. Mix well and dispense into sterile containers.

Storage conditions and Shelf life
Novobiocin Supplement SR0181 should be stored in the dark at 2-8°C. When stored as directed, the supplement remains stable until the expiry date printed on the label.

Nutrient agar (500g, Oxoid) is a culture media that used to cultivate and isolate fastidious pathogens and other microorganisms. This non-selective medium contains beef extracts combined with peptone, yeast extracts and sodium chloride to provide the nitrogen compounds, carbon, vitamins and some trace ingredients necessary for the growth of bacteria. A general-purpose medium which may be enriched with up to 10% blood or other biological fluid.

Nutrient Agar, Oxoid Composition

Nutrient Agar, Oxoid Preparation:

Powder: Suspend 28g of nutrient agar in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Nutrient Agar Tablets make 5mL of medium each.

Nutrient Agar Tablets, Oxoid Composition

Nutrient Agar, Oxoid Preparation:
Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Cultivate fastidious pathogens and other microorganisms with nutritious Thermo Scientific™ Oxoid™ Nutrient Broth No.2 (Dehydrated). This medium is comparable to a meat infusion and is richer in nutrients than Nutrient Broth (CM0001). It gives good growth from small inocula and is recommended for sterility testing for aerobic organisms. It complies with the recommendations in the ‘British Pharmacopoea’ for the composition of a sterility testing medium for aerobes.

Nutrient Broth No.2, Oxoid Composition

Nutrient Broth No.2, Oxoid Preparation:
Add 25g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Nutrient Gelatin is used for the differentiation of microorganisms by their proteolytic effects.

Nutrient Gelatin, Oxoid Composition

Nutrient Gelatin, Oxoid Preparation:
Suspend 128g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring and cool below 20°C or leave to set in a refrigerator.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not shake the gelatin tubes whilst they are warm because growth and liquefaction of gelatin frequently occurs on the surface layer only.
In routine diagnostic work report gelatin liquefaction or not. The type or shape of liquefaction is of less importance.

Oxoid OGYE Selective Supplement (Oxytetracycline Glucose Yeast Extract) is added to Oxytetracycline Glucose Yeast Extract Agar Base, Part No. CM0545B.

• Add to Oxytetracycline Glucose Yeast Extract Agar Base, Part No. CM0545B
• Each vial supplements 500mL of medium

OGYE Selective Supplement, Oxoid Composition

OGYE Selective Medium Preparation:
Suspend 18.5g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 10 minutes. Allow the medium to cool to 50°C and aseptically add the contents of one vial of Oxytetracycline GYE Selective Supplement (SR0073) reconstituted as directed. Mix thoroughly and pour into sterile Petri dishes. The pH of this medium should be 7.0±0.2 @ 25°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product has been discontinued, please refer to SR0258E

Thermo Scientific™ Oxoid OCLA (ISO) Differential Supplement is a differential supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Differential Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Thermo Scientific™ Oxoid OCLA (ISO) Selective Supplement is a selective supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Selective Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between E. coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies.

ONE Broth-Listeria Base, Oxoid Composition

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Base must be stored tightly capped in the original container at 10-30°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

Precautions
ONE Broth-Listeria Base and ONE Broth Selective Supplement are for Laboratory Use Only.
ONE Broth-Listeria Base is designated as “Irritant” and ONE Broth-Listeria Selective Supplement is designated as “Harmful”. For a full Material Safety Data Sheet (MSDS).
Do not use ONE Broth-Listeria Base or ONE Broth-Listeria Selective Supplement beyond the stated expiry dates, or if the products show any sign of deterioration.

Thermo Scientific™ Oxoid ONE Broth Salmonella Selective Supplement is a selective supplement for use with ONE Broth-Salmonella Base.

ONE Broth Salmonella Selective Supplement, Oxoid Composition

ONE Broth Salmonella Selective Medium Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
The ONE Broth-Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

The Oxoid ONE Broth-Listeria is for the selective enrichment broth for Listeria species from food samples in 24 hours.

One Broth-Listeria Selective Supplement
ONE Broth-Listeria is the selective enrichment broth used in the Oxoid Listeria Precis™ rapid culture method. For information simply download the ONE Broth-Listeria data sheet (273KB) or the Listeria Precis data sheet (680KB) in PDF format.

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Selective Supplement should be stored in the dark at 2-8°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

Enrich and recover Salmonella spp. from food samples in a single step with Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Base, when prepared with Thermo Scientific Oxoid ONE Broth-Salmonella Selective Supplement (SR0242).

ONE Broth-Salmonella Base, Oxoid Composition

ONE Broth-Salmonella Base, Oxoid Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
ONE Broth-Salmonella should be stored tightly capped in the original container at 10-30ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
ONE Broth-Salmonella medium and Brilliance Salmonella Agar are for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration. Sterilize specimens, equipment and media properly after use; dispose waste in accordance with local rules and regulations. Directions should be read and followed carefully.

Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Supplement is for the selective enrichment of Salmonella spp. from food samples in 24 hours in a single enrichment step.

• Add to ONE Broth-Salmonella Part No. CM1091B
• Each vial supplements 500mL of medium

ONE Broth Salmonella Selective Supplement, Oxoid Composition

ONE Broth Salmonella Selective Medium Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
The ONE Broth-Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.