Culture Media Products

• Oxoid K-F Streptococcus Agar is a selective medium for the isolation and enumeration of Group D Streptococci.
• Also available TTC Solution, Part No. SR0148A.

K-F Streptococcus Agar Base, Oxoid Composition

K-F Streptococcus Agar, Oxoid Preparation:
Suspend 38.2g in 500ml of distilled water. Bring to the boil with frequent agitation. Boil for 5 minutes. Cool to 50°C and add aseptically 1 vial (5ml) of 1% 2,3,5-Triphenyltetrazolium chloride (SR0229K). Pour into sterile Petri dishes when using the membrane filtration method or hold at 45°C when using the pour-plate method.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
Observe the HAZARD precautions regarding sodium azide when disposing of the medium. The pH of the medium should not fall below 7.0 as it may become inhibitory towards enterococci. KF Streptococcus Agar is not specific for the presumptive identification of Group D streptococci. Further tests must be made to confirm the identity of the organisms isolated.

• Oxoid Kanamycin Aesculin Azide Agar Base is a selective medium for the isolation of Enterococci in foodstuffs.
• Also available Kanamycin Sulphate Selective Supplement, Part No. SR0092E.

Kanamycin Aesculin Azide Agar Base, Oxoid Composition

Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Suspend 21.3g in 500ml of distilled water. Add one vial of Kanamycin Supplement (SR0092) reconstituted as directed. Bring to the boil. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.

Precautions
Observe the hazard precautions regarding sodium azide when disposing of the medium.
There is no universal medium which will isolate all strains of enterococci.
Unless a presumptive count is acceptable all isolates should have their identity confirmed with further tests.

Oxoid Kanamycin Sulphate Supplement (10mg/vial) is added to Kanamycin Esculin Azide Agar Base, Part No. CM0591B.

Kanamycin Sulphate Supplement, Oxoid Composition

Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Add 2ml of sterile distilled water to one vial and mix gently to dissolve completely. Add the contents to 500ml of Kanamycin Aesculin Azide Agar Base.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.

• Oxoid Campylobacter Karmaili Agar Base is a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42°C.
• Also available Campylobacter Selective Supplement (Karmaili), Part No. SR0167E and Modified Karma-i-li Selective Supplement, Part No. SR0205E.

Campylobacter Karmaili Agar Base, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Add 21.5g of Campylobacter Agar Base (Karmali) to 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of Campylobacter Selective Supplement (Karmali) SR0167 or Modified Karmali Selective Supplement SR0205, reconstituted as directed.. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Oxoid Kligler Iron Agar is used for differential identificaton of Enterobacteriaceae.

Kligler Iron Agar, Oxoid Composition

Kligler Iron Agar, Oxoid Preparation:
Suspend 55g in 1 litre of distilled water. Bring to the boil to dissolve completely. Mix well and distribute into containers. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set as slopes with 1 inch butts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is essential that Kligler Iron Agar is examined and reported at 18-24 hours. Early or late readings will give false results.
KIA will grow both oxidative and fermentative organisms. Confusion will result if care is not taken to distinguish between the two groups.
Always use a straight wire to inoculate the butt, to avoid splitting the agar with a loop.
Pure cultures are essential to avoid misinterpretation.
Do not use screw-capped tubes or bottles for KIA medium. It is essential that air is freely available to growth on the slant.

Oxoid Lab-Lemco Broth is a nutrient medium equivalent to USA nutrient extract formulations.

Lab-Lemco Broth, Oxoid Composition

Lab-Lemco Broth, Oxoid Preparation:
Dissolve 8g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.
`Lab-Lemco’ Broth is slightly more alkaline than the medium used in the APHA publications (pH 6.8).

Thermo Scientific™ Oxoid Lab-Lemco Powder is a Beef Extract made from specially selected raw materials.

• May be used in fermentation processes
• Industrial Reference: NMKL

Oxoid Lactalbumin Hydrolysate is a pancreatic digest of lactalbumin which contains high levels of essential amino acids.

Thermo Scientific™ Oxoid Lactic Acid is a solution for addition to culture media, primarily to lower the pH.

Thermo Scientific™ Oxoid Lactic Acid is a solution for addition to culture media, primarily to lower the pH.

Oxoid Lactose Bacteriological is a sugar suitable for inclusion in bacteriological culture media.

Presumptively identify coliform organisms in milk, water and foods as specified by the American Public Health Association with Thermo Scientific™ Oxoid™ Lactose Broth (Dehydrated). Beef extracts and peptone in this liquid medium supply essential nutrients while lactose is a fermentable carbohydrate for coliforms. Coliforms ferment lactose in the medium resulting in gas formation which is indicative of positive results.

Lactose Broth, Oxoid Composition

Lactose Broth, Oxoid Preparation:
Dissolve 13g in 1 litre of distilled water and distribute into containers with fermentation tubes (Durham). Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature (18-22°C).

Precautions
Ensure that the fermentation tubes are free from air bubbles before inoculation.
Large water samples may require double-strength lactose broth to reduce the final volumes. Do not overheat double- strength broth or inhibitory products will be produced.

Detect coliform organisms in water, waste water and dairy products with selective Thermo Scientific™ Oxoid™ Lauryl Tryptose Broth (Dehydrated), while conforming to the formula detailed by American Public Health Association. Lauryl Tryptose Broth is designed to promote a rich growth and copious gas production from small inocula of coliform organisms.

Lauryl Tryptose Broth, Oxoid Composition

Lauryl Tryptose Broth, Oxoid Preparation:
Dissolve 35.6g in 1 litre of distilled water and distribute into containers with fermentation tubes (Durham). Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Precautions
If stored at 2-8°C the broth will become cloudy or form a precipitate. This should clear at room temperature but gas formation is the criterion of growth not turbidity.

Oxoid Lauryl Tryptose Broth w/MUG is used for the presumptive identification of E. coli.

Lauryl Tryptose Broth with MUG, Oxoid Composition

Lauryl Tryptose Broth, Oxoid Preparation:
Suspend 35.65g of Lauryl Tryptose Broth with MUG in 1 litre of distilled water. Warm to 40°C. Mix well to dissolve and distribute into final containers holding inverted Durham tubes. (Please be aware of the special glassware requirements when using ultra-violet light.). Sterilise by autoclaving at 121° C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Description

LB Agar Miller from Merck is a commonly used medium in microbiology laboratories and is suitable for routine bacterial culture and transformation experiments. It is a formulation of Luria-Bertani (LB) agar developed by Miller, which includes a mixture of peptides, amino acids, and vitamins to support the growth of a wide range of bacteria.

Oxoid LCAT Selective Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

LCAT Selective Supplement, Oxoid Composition

LCAT Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 490ml of sterile GC Agar Base CM0367, cooled to approximately 50°C, containing 50 ml Laked Horse Blood SR0048 and the rehydrated contents of one vial of Yeast Autolysate Supplement SR0105. Mix gently and pour into sterile Petri dishes.

Oxoid Legionella GVPC Selective Supplement is used for the isolation of Legionella spp. from environmental samples.

• Add to Legionella CYE Agar Base, Part No. CM0655B
• Each vial supplements 500mL of medium

Legionella GVPC Selective Supplement, Oxoid Composition

Legionella GVPC Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Legionella CYE Agar Base CM0655 to which one vial of Legionella BCYEa Legionella Growth Supplement SR0110C has been added cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

• Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
• Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

• Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
• Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Oxoid Legionella BCYE Supplement w/o L-cysteine is used for the isolation of Legionella spp.

• Add to Legionella CYE Agar Base, Part No. CM0655B
• Each vial supplements 100mL of medium

Legionella BCYE Growth Supplement without L-cysteine, Oxoid Composition

Legionella BCYE Growth Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90ml of sterile Legionella CYE Agar Base (CM0655) cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Thermo Scientific™ Oxoid Legionella BMPA-α Selective Supplement is a selective supplement for the isolation of legionellae when used with Legionella BCYE Growth Supplement (SR0010) and Legionella CYE Agar Base (CM0655).

Legionella BMPA-α Selective Supplement, Oxoid Composition

Legionella BMPA-α Selective Medium Preparation:
Reconstitute one vial SR0111E per 100ml medium as directed. Aseptically add the vial contents to BCYE-a Medium prepared from Legionella CYE Agar Base (CM0655) and Legionella BCYE Supplement (SR0110) and cooled to 50°C. Mix well and pour into sterile Petri dishes.

Isolate Legionella from environmental samples and clinical specimens using Thermo Scientific™ Oxoid™ Legionella CYE Agar Base when used with Legionella Growth Supplement SR01110. Legionella CYE Agar Base is a non-selective medium that exhibits optimal recovery of legionellaceae in short incubation periods from both environmental samples and clinical specimens.

Legionella CYE Agar Base, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

• Oxoid Listeria Enrichment Broth is used for the enrichment of Listeria species.
• Also available Listeria Selective Supplement, Part No. SR0140E or SR0141E and Modified Listeria Selective Enrichment Supplement, Part No. SR0206E or SR0206B.

Listeria Enrichment Broth Base, Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 18g in 500ml of distilled water. Add the contents of one vial of Listeria Selective Enrichment Supplement (SR0141), reconstituted with 2ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140. Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against Listeria.
Supplement SR0141 used in this medium contains a toxic concentration of cycloheximide. Note the precautions to be taken under HAZARDS.