Microbiology

• Oxoid Campylobacter Selective Supplement, Skirrow is used for preparation of Skirrow Selective Medium for the isolation of Campylobacter spp. at 42°C.
• Add to Blood Agar Base No. 2, Part No. CM0271B or Columbia Blood Agar Base, Part No. CM0331B.

Campylobacter Selective Supplement, Skirrow, Oxoid Composition

Campylobacter Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed aseptically add the contents to 500 ml of a nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2 , with 10% defibrinated horse or sheep blood SR0050,SR0051 or 5-7% laked horse blood SR0048. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Cary-Blair Transport Medium is a transport medium for gram-negative and anaerobic microorganisms.

Cary-Blair Transport Medium, Oxoid Composition

Cary-Blair Transport Medium, Oxoid Preparation:
Suspend 13.3g in 1 litre of distilled water and bring gently to the boil to dissolve the agar. Distribute into small, screw-cap bottles and sterilise by immersing in free-steam for 15 minutes. Allow to cool and tighten the screw caps to prevent water loss.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at 2-8°C or at room temperature (22-25°C) up to 19 months.

Precautions
The medium should not be incubated to check sterility, prior to use. This should be carried out on separate quality control samples.
The medium can maintain the viability of fastidious organisms for transport purposes but it should not be used as a storage or enrichment medium.
The results obtained from the medium are dependent on the quality of the specimen material. Commensal anaerobic organisms may overgrow in the medium and cause misleading results.

Oxoid Casein Hydrolysate (Acid) is a purified hydrolysate which provides the necessary nitrogenous material for culture media or can be used when amino acid mixtures are specified.

Oxoid CCDA Selective Supplement is used for the isolation of Campylobacter spp., especially C. upsaliensis or Arcobacter.

• For the isolation of Arcobacter spp., add to Arcobacter Broth Base, Part No. CM0965B
• For the isolation of Campylobacter spp., especially C. upsaliensis, add to Campylobacter Blood-Free Selective Agar Base (CCDA Preston), Part No. CM0739B

CCDA Selective Supplement, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Oxoid CCDA Selective Supplement is used for the isolation of Campylobacter spp., especially C. upsaliensis or Arcobacter.

• For the isolation of Arcobacter spp., add to Arcobacter Broth Base, Part No. CM0965B
• For the isolation of Campylobacter spp., especially C. upsaliensis, add to Campylobacter Blood-Free Selective Agar Base (CCDA Preston), Part No. CM0739B

CCDA Selective Supplement, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Oxoid CDMN Supplement is used for the growth of Clostridium difficile.

• Add to to C. Difficile Agar Base, Part No. CM0601B
• Each vial for 2L medium

CDMN Selective Supplement, Oxoid Composition

CDMN Agar, Oxoid Preparation:
Aseptically add 2ml of sterile distilled water to one vial of supplement and mix gently to dissolve completely. Aseptically add the contents to 500ml of sterile Clostridium difficile Agar Base CM0601 cooled to approximately 50°C together with 7% (v/v) of Defibrinated Horse Blood SR0050. Mix well and pour the resulting CDMN Agar into sterile Petri dishes.

Oxoid Cefixime Supplement is used for the isolation of Escherichia coli O157.

• Add to to Cefixime Rhamnose Sorbitol MacConkey Agar Base, Part No. CM1005B
• Each vial supplements 500mL of medium

Cefixime Supplement, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Oxoid C-T Supplement is a chromogenic medium for the identification of Escherichia coli O157:H7.
• Add to Sorbitol MacConkey Agar, Part No. CM0813B or to Sorbitol MacConkey Agar with BCIG, Part No. CM0981B.

Cefixime Tellurite Selective Supplement, Oxoid Composition

Sorbitol MacConkey Agar, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Sorbitol MacConkey Agar Base CM0813 cooled to approximately 50°C. Mix well and pour the medium into Petri dishes.

Storage conditions and Shelf life
Cefixime-Tellurite Supplement (SR0172) should be stored in the dark at temperatures below 0°C.

Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.

Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.