Culture Media Products

Isolate and enumerate spoilage organisms of citrus products with Thermo Scientific™ Oxoid™ Orange Serum Agar (Dehydrated). Orange Serum Agar is developed specifically for the isolation and enumeration of microorganisms that are capable of surviving citrus products. The low pH of these products limits the growth of microorganisms to those capable of tolerating the acidic environment.

Orange Serum Agar, Oxoid Composition

Orange Serum Agar, Oxoid Preparation:
Suspend 37g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

Oxoid ORSAB Selective Supplement (Oxacillin Resistance Screening Agar Base Selective Supplement) is added to Oxacillin Resistance Screening Agar Base, Part No. CM1008B.

• Add to Oxacillin Resistance Screening Agar Base, Part No. CM1008B
• Each vial supplements 500mL of medium

ORSAB Selective Supplement, Oxoid Composition

ORSAB Selective Medium Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
ORSAB Selective Supplement should be stored at 2-8°C.
Store the prepared medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

• Oxoid Oxacillin Resistance Screening Agar Base (ORSAB) is a medium for screening clinical samples for the presence of oxacillin-resistant Staphylococci.
• Also available ORSAB Selective Supplement, Part No. SR0195E.

Oxacillin Resistance Screening Agar Base (ORSAB), Oxoid Composition

Oxacillin Resistance Screening Agar, Oxoid Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid MUG Supplement (50mg) Fluorescent agent is used for the detection of E. coli.

• Contains 4-methylumbelliferyl-β-D-glucuronide (MUG)
• Each vial supplements 500mL of medium

M.U.G. Supplement, Oxoid Composition

M.U.G. Supplement, Oxoid Preparation:

Add 2ml of distilled water to a vial and invert gently until completely dissolved. Add the vial contents to the following volumes of suggested media, before sterilisation.

Storage conditions and Shelf life
Should be stored at 2-8°C. When stored as directed the unopened vial is stable until the expiry date on the label.

Precautions
The presence of endogenous glucuronidase in shellfish samples may result in false positive fluorescence. Test tubes used in the MPN method should be checked under UV light to ensure the glass does not fluoresce. To avoid false positive fluorescence the source of long wave UV light must not exceed 6 watts.

Selectively enumerate yeasts and molds from foodstuffs with Thermo Scientific™ Oxoid™ Oxytetracycline Glucose Yeast Extract Agar Base (Dehydrated). The medium is based upon the formulation described by Mossel et al. which utilizes oxytetracycline selectivity to provide increased counts of yeasts and molds compared to other media that utilize a low pH as a selective agent. Improve the recovery of physically stressed yeast cells on this neutral pH medium.

Oxytetracycline Glucose Yeast Extract Agar Base (Dehydrated), Oxoid Composition

Oxytetracycline Glucose Yeast Extract Agar, Oxoid Preparation:
Suspend 18.5g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 10 minutes. Allow the medium to cool to 50°C and aseptically add the contents of one vial of Oxytetracycline GYE Selective Supplement (SR0073) reconstituted as directed. Mix thoroughly and pour into sterile Petri dishes. The pH of this medium should be 7.0±0.2 @ 25°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions:
The lactic-acid bacteria are inhibited on this medium.

• Oxoid PALCAM Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
• Also available PALCAM Selective Supplement, Part No. SR0150E or SR0150B.

PALCAM Agar Base, Oxoid Composition

PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.

The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.

Precautions
Acriflavine hydrochloride is activated by light which may cause it to become inhibitory to Listeria growth.

Thermo Scientific™ Oxoid PALCAM Supplement is a selective supplement for use with PALCAM Agar Base.

PALCAM Supplement, Oxoid Composition

PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.

The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.

• Oxoid Perfringens OPSP Agar Base is used for the enumeration of Clostridium perfringens in foods.
• lso available Perfringens OPSP Supplement A, Part No. SR0076E and Perfringens OPSP Supplement B, Part No. SR0077E.

Perfringens OPSP Agar Base, Oxoid Composition

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The production of black colonies on this medium is a presumptive identification of Clostridium perfringens. Further identification tests must be carried out.

A Bacillus cereus 569/H9 lactamase (E.C.5.2.6) presented as a freeze-dried powder containing buffer salts. Each vial contains a 3,300lU of activity (1 unit of enzyme activity will hydrolyse 1.0μmol of benzylpenicilloic acid per minute pH7.0 and at 25°C).

Used for the inactivation of susceptible beta-lacram antibiotics in preparations, blood or tissue samples.

The Oxoid Peptone Bacteriological is for use whenever a high quality bacteriological peptone is required.

Oxoid Peptone Bacteriological Neutralized is the neutralized form of Peptone Bacteriological.

Oxoid Peptone Mycological is a mycological peptone developed for isolation of pathogenic and nonpathogenic fungi.

Thermo Scientific™ Oxoid Peptone P is a peptic digest which conforms to the USP specification of peptic digests of animal tissue.

• The high sulphur content makes it ideal for use in culture media

Oxoid Peptone Special is a specifically prepared mixture of peptones designed to encourage growth of the most fastidious microorganisms.

Peptone water (CM0009B), Oxoid, is a basal medium that used as a growth medium or as the basis of carbohydrate fermentation media. A pure culture in Peptone Water is a convenient inoculum for a series of fermentation tubes or other diagnostic media.

Directions

Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Oxoid Perfringens OPSP Selective Supplement A is for selective isolation of Clostridium perfringens from food.

• Add to Perfringens Agar Base, OPSP, Part No. CM0543B
• For 500mL of medium

Perfringens OPSP Selective Supplement A, Oxoid Composition

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Perfringens SFP Supplement is for the selective isolation of Clostridium perfringens.

• Add to Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens SFP Supplement, Oxoid Composition

Shahidi-Ferguson Perfringens Agar (SFP Agar) Preparation:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Perfringens TSC Supplement (D-cycloserine 200mg/vial) is used for the recovery of Clostridium perfringens.

• Add to DCLS Agar, Part No. CM0393B, or Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens TSC Supplement, Oxoid Composition

Agar Preparation:
To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.

To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Selectively isolate and presumptively identify Clostridium perfringens in food samples with Thermo Scientific™ Oxoid™ Perfringens TSC Agar Base (Dehydrated). The medium can either be used on its own or with selective agents to make Tryptose Sulphite (TS) Agar, Tryptose Sulphite Cycloserine (TSC) agar or Shahadi Ferguson Perfringens (SPF) agar for the presumptive identification and enumeration of Clostridium perfringens.

Perfringens TSC/SFP Agar Base, Oxoid Composition

Agar Preparation:
To Prepare the Agar Base:
Suspend 23g in 500ml of distilled water and heat gently until the agar is completely dissolved. Sterilise by autoclaving at 121°C for 10 minutes. Allow the medium to cool to 50°C.

To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.

To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.

To Prepare Shahidi-Ferguson Perfringens Agar (SFP Agar):
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.

To Prepare Agar for an Overlay:
For TSC or SFP Agar used as an overlay, the egg yolk emulsion (SR0047) is omitted. Its inclusion does not improve the lecithinase reaction and diminishes the visibility of the colonies.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Black colonies appearing on these two media may be organisms other than Clostridium perfringens.

Oxoid Perfringens OPSP Selective Supplement B is for selective isolation of Clostridium perfringens from food.

• Add to Perfringens Agar Base, OPSP, Part No. CM0543B
• For 500mL of medium

Perfringens OPSP Selective Supplement B, Oxoid Composition

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Determine the plate counts of microorganisms in food, dairy samples, waste water and other material of sanitary importance with Thermo Scientific™ Oxoid™ Plate Count Agar (Dehydrated). Plate Count Agar was developed by Buchbinder et al which is recommended by APHA, AOAC and PHLS. The quality control of Plate Count Agar (APHA) includes testing in accordance with ISO 11133:2014.

Plate Count Agar (Dehydrated), Oxoid Composition

Plate Count Agar (Dehydrated), Oxoid Preparation:
Add 17.5g to 1 litre of distilled water. Dissolve by bringing to the boil with frequent stirring, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Isolate and enumerate microorganisms in milk and dairy products with Thermo Scientific™ Oxoid™ Milk Plate Count Agar (Dehydrated). Milk Plate Count Agar is equivalent to the medium recommended by British Standard Institution and International Organization for Standardization.

Milk Plate Count Agar (Dehydrated), Oxoid Composition

Milk Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 19.5g in 1 litre of distilled water. Bring to the boil with frequent agitation, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Bacillus Cereus Selective Supplement (Polymyxin B Supplement) is supplied 50,000 IU/Vial for inhibition of Gram-negative bacteria.

• May be added to Bacillus cereus Selective Agar Base, Part No. CM0617B and MYP Agar Base, Part No. CM0929B
• Each vial supplements 500mL of medium

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Composition

Bacillus Cereus Selective Supplement (Polymyxin B Supplement), Oxoid Preparation:
Reconstitute as directed in the product instructions for use. Aseptically, add one vial to 500ml of liquid or molten base medium, which is below 50°C. Mix well, and dispense into appropriate, sterile containers.

Storage conditions and Shelf life
Polymixin B Supplement at 2-8°C and use before the expiry date on the label.