Culture Media Products

Oxoid VCNT Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B

VCNT Selective Supplement, Oxoid Composition

VCNT Selective Medium Preparation:
Aseptically add 2ml of sterile distilled water to one vial mix gently to dissolve the powder completely. Aseptically add the contents of one vial to 500ml of sterile Thayer Martin Medium, cooled to approximately 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 and the rehydrated contents of one vial of Vitox SR0090.

Isolate and enumerate coliforms in food, animal feed and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), while adhering to ISO 4832:2006 and ISO/TS 11133-2:2003. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBL Agar (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBL Agar (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBL Agar (ISO) plates can be stored for up to 2 weeks when made from CM0968 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBL Agar (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBL Agar (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.

• Each vial supplements 500mL of medium
• Format: Freeze dried

Vancomycin Supplement, Oxoid Composition

Storage conditions and Shelf life
Vancomycin Supplement SR0186 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.

• Each vial supplements 500mL of medium

Vancomycin Supplement, Oxoid Composition

Cronobacter Screening Broth (CSB), Oxoid Preparation:
Dissolve 14.02g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Thermo Scientific™ Oxoid Vegetable Peptone No. 1 is a highly nutritious, meat-free general purpose peptone for growth of bacteria and fungi.

Detect and enumerate Enterobacteriaceae in food products with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose Agar (Dehydrated). This glucose-containing selective medium gives reproducible counts of typical colonies for food safety analysis. In the examination of food stuffs, detection of the Enterobacteriaceae that ferment glucose to produce acid and/or gas has been recommended.

Violet Red Bile Glucose Agar (Dehydrated), Oxoid Composition

Violet Red Bile Glucose Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well and dispense into tubes or dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms may grow e.g. Aeromonas. The selective activity of this medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth. Medium for the pour-plate procedure should be freshly prepared, cooled to 47°C and used within 3 hours.

This item is not available at the moment. Please refer to the alternative, Violet Red Bile Glucose Agar (CM0485B). However, please note that this product does not conform to ISO.

Selectively enumerate Enterobacteriaceae in food, animal feed, and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated) while adhering to ISO 21528 and ISO 11133:2014. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Glucose (VRBG) Agar (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBGA (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBGA (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBGA (ISO) plates can be stored for up to 2 weeks when made from CM1082 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBGA (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBGA (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

Detect and enumerate coliform organisms in water, food and dairy products with Thermo Scientific™ Oxoid™ Violet Red Bile Agar (Dehydrated). This lactose containing selective medium has been used for the determination of the coli-aerogenes content of water, milk and other dairy products, dairy equipment, and food products. Due to the addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Agar (Dehydrated), Oxoid Composition

Violet Red Bile Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms e.g. Aeromonas and Yersinia species may give similar reactions.
The selectivity of the medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth.

Oxoid Vitox Supplement is used for the preparation of Thayer Martin Medium and New York City Medium for the isolation of Neisseria spp.

• Contents: Vitox Supplement (5 vials) plus reconstitution fluid (5 vials)
• Add to GC Agar Base, Part No. CM0367B

Ordering Information:

• Each vial of Vitox plus reconstitution fluid supplements 500mL of medium

Includes:

• Five vials of Vitox plus five vials of reconstitution fluid

Vitox Supplement, Oxoid Composition

Vitox Rehydration Fluid , Oxoid Composition

Vitox, Oxoid Preparation:
Aseptically add one vial of Hydration Fluid to one vial of Vitox. Mix gently to dissolve completely. Aseptically add the contents of one vial to sterile medium of choice, cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid Vitox Supplement is used for the preparation of Thayer Martin Medium and New York City Medium for the isolation of Neisseria spp.

• Contents: Vitox Supplement (5 vials) plus reconstitution fluid (5 vials)
• Add to GC Agar Base, Part No. CM0367B

Ordering Information:

• Each vial of Vitox plus reconstitution fluid supplements 500mL of medium

Includes:

• Five vials of Vitox plus five vials of reconstitution fluid

Vitox Supplement, Oxoid Composition

Vitox Rehydration Fluid , Oxoid Composition

Vitox, Oxoid Preparation:
Aseptically add one vial of Hydration Fluid to one vial of Vitox. Mix gently to dissolve completely. Aseptically add the contents of one vial to sterile medium of choice, cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid Vogel-Johnson Agar is used for the detection of Staphylococcus aureus from foods and clinical specimens.

Vogel-Johnson Agar, Oxoid Composition

Vogel-Johnson Agar, Oxoid Preparation:
Suspend 61g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5.7ml of sterile 3.5% potassium tellurite solution SR0030 (equivalent to 20ml of 1% potassium tellurite).

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
All presumptive Staphylococcus aureus colonies should be confirmed with further tests. Do not heat the medium after the addition of potassium tellurite.

Oxoid Violet Red Bile Agar w/MUG is used for the presumptive identification of E. coli.

Violet Red Bile Agar with MUG, Oxoid Composition

Violet Red Bile Agar with MUG, Oxoid Preparation:
Suspend 38.6g of VRBA with MUG in 1 litre of distilled water. Bring to the boil to dissolve completely. No further sterilisation is necessary or desirable. Mix well before pouring into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.

Oxoid VRE Agar Base (Vancomycin Resistant Enterococci Agar Base) is used for selective isolation of vancomycin-resistant enterococci and high level aminoglycoside-resistant enterococci (HLARE) from clinical samples.Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Agar Base, Oxoid Composition

VRE Agar, Oxoid Preparation:
Suspend 42.6g of VRE Agar Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121° C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then Mix agar well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.
Warning: Sodium azide is harmful if swallowed, please take all necessary precautions.

This product has been discontinued. Please contact us for more information.

• Oxoid VRE Broth Base (Vancomycin Resistant Enterococci Broth Base) is for selective isolation of vancomycin-resistant enterococci and high Level aminoglycoside-resistant enterococci.
• Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Broth Base, Oxoid Composition

VRE Broth, Oxoid Preparation:
Suspend 37.0g of VRE Broth Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121°C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then mix well and distribute into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.

• Oxoid WL Nutrient Agar is used for the determination of the microbiological flora in brewing and fermentation processes.
• Also available Cycloheximide 0.1% Solution, Part No. SR0222C.

WL Nutrient Agar, Oxoid Composition

WL Nutrient Agar, Oxoid Preparation:
Suspend 75g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. If required the pH may be adjusted to 6.5 by the addition of 1% sodium bicarbonate solution.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
When handling cycloheximide observe the precautions to be taken under HAZARDS.

Numerate all culturable microorganisms from water using colony count technique with Thermo Scientific™ Oxoid™ Water Plate Count Agar (Dehydrated). This nutritious, non-selective medium conforms to ISO 6222: 1999 (E) Water quality ― Enumeration of culturable microorganisms ― Colony count by inoculation in a nutrient agar culture medium1.

Water Plate Count Agar (Dehydrated), Oxoid Composition

Water Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 24g of Water Plate Count Agar (ISO) in 1 litre of distilled water and bring gently to the boil to dissolve. If desired, distribute volumes of 15ml to 20ml in tubes, bottles or other containers. Sterilise by autoclaving at 121°C for 15 minutes. After sterilisation, allow to cool and maintain at 45 ± 1°C using a water bath.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Oxoid Wilkins-Chalgren Anaerobe Agar is used for the growth of anaerobes and recommended for antimicrobial susceptibility testing of anaerobes.

Wilkins-Chalgren Anaerobe Agar, Oxoid Composition

Wilkins-Chalgren Anaerobe Agar, Oxoid Preparation:
Suspend 43g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

Wort Agar is a general purpose mycological medium, equivalent to the medium described by Parfitt and especially suitable for the cultivation and enumeration of yeasts. The medium which duplicates the composition of natural wort, is of an acidity which is optimal for many yeasts but inhibitory to most bacteria. Parfitt investigated the relative merits of wort agar and other media for the count of yeasts and moulds in butter, and recommended the use of dehydrated whey, malt or wort agar for the purpose. Scarr employed a modified wort agar (’osmophilic agar’) for the examination of sugar products for osmophilic yeasts. Scarr’s technique is also used for the determination of osmophilic yeasts occurring in materials used in the manufacture of soft drinks.

Wort Agar, Oxoid Composition

Wort Agar, Oxoid Preparation:
Suspend 50g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
PROLONGED OR EXCESSIVE HEATING WILL DIMINISH THE GEL STRENGTH OF THE AGAR.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not remelt the solid agar, it will destroy the gel. The surface of the agar is soft but suitable for poured inocula.

Isolate Salmonella and Shigella from clinical specimens and foods with selective Thermo Scientific™ Oxoid™ XLD Agar (Dehydrated). The medium relies on xylose fermentation, lysine decarboxylation and production of hydrogen sulphide for the primary differentiation of shigellae and salmonellae from non-pathogenic bacteria. The careful addition of sodium desoxycholate inhibits the growth of coliforms without decreasing the ability to support shigellae and salmonellae.

XLD Agar (Dehydrated), Oxoid Composition

XLD Agar (Dehydrated), Oxoid Preparation:
Suspend 53g in 1 litre of distilled water. Heat with frequent agitation until the medium boils. DO NOT OVERHEAT. Transfer immediately to a water bath at 50°C. Pour into sterile Petri dishes as soon as the medium has cooled.

It is important to avoid preparing large volumes which will cause prolonged heating.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Isolate Salmonella and Shigella from clinical specimens and foods with selective Thermo Scientific™ Oxoid™ XLD Agar (Dehydrated). The medium relies on xylose fermentation, lysine decarboxylation and production of hydrogen sulphide for the primary differentiation of shigellae and salmonellae from non-pathogenic bacteria. The careful addition of sodium desoxycholate inhibits the growth of coliforms without decreasing the ability to support shigellae and salmonellae.

XLD Agar (Dehydrated), Oxoid Composition

XLD Agar (Dehydrated), Oxoid Preparation:
Suspend 53g in 1 litre of distilled water. Heat with frequent agitation until the medium boils. DO NOT OVERHEAT. Transfer immediately to a water bath at 50°C. Pour into sterile Petri dishes as soon as the medium has cooled.

It is important to avoid preparing large volumes which will cause prolonged heating.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Efficiently isolate and identify salmonellae from clinical, environmental and food samples with highly selective Thermo Scientific™ XLT-4 Agar. XLT-4 Agar contains sodium tetradecylsulfate which acts to inhibit many of the background flora organisms whose growth can cause problems with differentiation on other Salmonellae plating media. This enhanced selectivity allows a significant increase in the recovery of salmonellae, while reducing false-positive suspect colonies, saving time and reducing the costs of further identification of false-positive colonies.

XLT-4 Agar Base, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid XLT-4 Selective Supplement is a selective supplement for use with XLT-4 Agar.

XLT-4 Selective Supplement, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid Yeast and Mold Agar is used for the isolation and maintenance of yeast and molds.

Yeast and Mold Agar, Oxoid Composition

Yeast and Mold Agar, Oxoid Preparation:
Suspend 41.0g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. The medium may be rendered selective after sterilisation by acidifying to pH 4.0 with 12-15ml of Lactic Acid SR0021 after cooling to 50°C. Do not reheat after making this addition. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
For in vitro diagnostic use.
Do not use beyond the stated expiry date or if the product is caked, discoloured or shows any sign of deterioration.

Oxoid Yeast Autolysate Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

Yeast Autolysate Supplement, Oxoid Composition

Yeast Autolysate Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053. Mix gently and pour into sterile Petri dishes.