Culture Media Products

Oxoid Bile Salts No. 3 is a specifically refined fraction of bile acid salts.

• Normal working concentration 0.15% w/v.
• Each vial supplements 500mL of medium.

Isolate Salmonella typhi and other salmonllae from pathological material, sewage water supplies, food and other products with Thermo Scientific™ Oxoid™ Bismuth Sulphite Agar (Modified Wilson & Blair Medium) (Dehydrated). Bismuth Sulphite Agar is a modification of the original Wilson and Blair Medium and is particularly useful for the isolation of lactose-fermenting salmonellae. The presence of bismuth sulphite and brilliant green make this medium highly selective for salmonellae.

Bismuth Sulphite Agar, Oxoid Composition

Bismuth Sulphite Agar, Oxoid Preparation:
Suspend 20g in 500ml of distilled water in a 1 litre flask. Heat gently with frequent agitation until the medium just begins to boil and simmer for 30 seconds to dissolve the agar. Cool to 50-55°C, mix well to disperse suspension and pour thick plates (25 ml medium per plate). Allow the medium to solidify with the dish uncovered. Larger volumes may be prepared if great care is taken and adequate head space provided.
Dry the plates before use but take care to avoid overdrying. Correctly prepared plates should have a smooth, cream-like opacity with a pale straw colour. There should be no sedimentation of the indicator.
DO NOT OVERHEAT – DO NOT AUTOCLAVE

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Note the following comments:
Due to its contents of reactive and hygroscopic substances, dehydrated Bismuth Sulphite Agar quickly deteriorates when exposed to the atmosphere. This is usually indicated by aggregation into a solid non-friable mass, and by the development of a brown coloration. Medium reconstituted from such material is brown, does not become green on storage, and is characterised by loss of differential and selective properties. For this reason the powder should be stored in a cool, dry place and after use the container should be properly closed.

Precautions
Prepared plates of medium should not be stored for longer than two days at 2-8°C; after which time the dye oxidises to give a green medium that can be inhibitory to some salmonellae.

Shigella species are usually completely inhibited.

Salmonella sendai, Salmonella cholera-suis, Salmonella berta, Salmonella gallinarum and Salmonella abortus-equi are markedly inhibited.
It is important that the spreading technique yields well separated colonies. The typical colonial characteristics will not develop if the growth is too heavy or confluent; Salmonella typhi colonies will appear light green in these circumstances. Therefore, when in doubt, almost any growth on the medium should be subject to further tests.

Oxoid Blood Agar Base is a nonselective general purpose medium which may be enriched with blood or serum.

Blood Agar Base, Oxoid Composition

Blood Agar Base, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

For blood agar, cool the Base to 50°C and add 7% of Defibrinated Horse Blood SR0050. Mix with gentle rotation and pour into petri dishes or other containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The haemolytic reactions of organisms inoculated on to this medium will be affected by the animal blood used e.g. horse or sheep and the incubation conditions e.g. aerobic, capnoeic or anaerobic.
When horse blood is added to the medium Haemophilus haemolyticus colonies will produce beta-haemolysis and mimic Streptococcus pyogenes.

Cultivate and isolate fastidious pathogens and other microorganisms with Thermo Scientific™ Oxoid™ Blood Agar Base No. 2 (Dehydrated). The specially designed formulation meets the need for a nutritious blood agar which permits maximum recovery of delicate organisms without interfering with their hemolytic reactions. Without any additions, the medium can be used for short-term maintenance of stock cultures. And with the addition of blood, the medium becomes suitable for the determination of the hemolytic reactions which are important diagnostic criteria for many organisms.

Blood Agar Base No. 2, Oxoid Composition

Blood Agar Base No. 2, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 7% sterile blood.

Mix with gentle rotation and pour into sterile dishes or other containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Brucella cultures are highly infective and must be handled under properly protected conditions. Incubate in 5-10% carbon dioxide atmosphere for 24-48 hours.

Isolate Campylobacter jejuni, Campylobacter coli and Compylobacter laridis using Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated) when used with CCDA Selective Supplement SR0155. Campylobacter Blood-Free Selective Agar Base is based on the original formulation described by Bolton et.al which was developed to replace blood with charcoal, ferrous sulphate and sodium pyruvate. For improved selectivity cephazolin in the formulation was replaced by cefoperazone as a selective agent.

Campylobacter Blood-Free Selective Agar Base, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Bolton Broth Base, Oxoid Composition

Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.

Oxoid Bolton Broth Selective Supplement is used for the selective pre-enrichment of Campylobacter spp. in food samples.

Add to to Bolton Broth, Part No. CM0983B. Each vial supplements 500mL of medium.

Bolton Broth Selective Supplement, Oxoid Composition

Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.

• Oxoid Bordetella Selective Supplement (Cephalexin, 20mg/vial) is used for the selective isolation of Bordetella pertussis and B. parapertussis.
• Add to Charcoal Agar Base, Part No. CM0119B. Each vial supplements 500mL of medium.

Bordetella Selective Supplement, Oxoid Composition

Bordetella Selective Medium, Oxoid Preparation:
Aseptically add 2ml of sterile distilled water to one vial and mix gently to dissolve the contents completely. Aseptically add the contents to 500ml of sterile molten Charcoal Agar CM0119, cooled to approximately 50°C, together with 10% defibrinated horse blood SR0050 . Mix well and pour into sterile Petri dishes.
The vial contents may also be added to half strength Charcoal Agar CM0119 plus 10% defibrinated horse blood SR0050, for use as a transport medium for Bordetella pertussis.

Cultivate fastidious organisms with highly nutritious Thermo Scientific™ Oxoid™ Brain Heart Infusion Agar (Dehydrated). Brain Heart Infusion Agar is recommended for the cultivation of Streptococci, Neisseria and other fastidious organisms.

Brain Heart Infusion Agar, Oxoid Composition

Brain Heart Infusion Agar, Oxoid Preparation:
Suspend 47g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes. For blood agar, cool to 50°C and enrich with 10% v/v sterile defibrinated blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The medium is tested for compatibility using 7% v/v oxalated horse blood, defibrinated horse blood or defibrinated sheep blood. There should be no evidence of lysis or darkening after incubation at 37°C, 25°C or 4°C for 72 hours.
When using this medium to isolate Histoplasma capsulatum, Coccidioides immitis or other pathogenic fungi which can produce free infective spores, extreme care must be taken to avoid dissemination of infective particles in the laboratory. The cultures should be examined only in a closed, filtered air cabinet.

This medium is unsuitable for the screening of vancomycin resistant enterococci (VRE). More suitable and superior media for the screening of VRE are available; Brilliance VRE PO1226A or Remel BHI with Vancomycin RO1176.

Cultivate streptococci, Neisseria and other fastidious organisms with Thermo Scientific™ Oxoid™ Brain Infusion Broth (Dehydrated). The medium is highly nutritious and well buffered to support the growth of wide variety of organisms. It is recommended for blood culture work for the isolation and cultivation of pathogenic fungi.

Brain Infusion Broth, Oxoid Composition

Brain Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store tubed or bottled medium in the dark and below 20°C.

Thermo Scientific™ Oxoid Brilliance Bacillus Cereus Agar is a chromogenic medium for the isolation and differentiation of Bacillus cereus from food samples.

Brilliance Bacillus Cereus Agar Base, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

Precautions
X-glucopyranoside negative B. cereus strains may present white colonies on this medium due to poor or no utilisation of the chromogen.

Brilliance Bacillus Cereus Agar Base, Oxoid is a chromogenic medium for the isolation and differentiation of Bacillus cereus from food samples.

• Also available Chromogenic Bacillus Cereus Selective Supplement, Part No. SR0230E.

Brilliance Bacillus Cereus Agar Base, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

Precautions
X-glucopyranoside negative B. cereus strains may present white colonies on this medium due to poor or no utilisation of the chromogen.

• Brilliance is a chromogenic medium for the isolation and differentiation of Bacillus cereus in food samples.
• Add to Brilliance Bacillus Cereus Agar Base, Part No. CM1036A and CM1036B. Each vial supplements 500mL of medium.

Brilliance Bacillus Cereus Selective Supplement, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Brilliance Candida Agar Base is a chromogenic medium for the isolation and differentiation of clinically important Candida spp., including C. albicans.
• Also available Chromogenic Candida Agar Selective Supplement, Part No. SR0231E.

Brilliance Candida Agar Base, Oxoid Composition

Brilliance Candida Agar, Oxoid Preparation:
Suspend 15.6g in 500ml of distilled water. Add 1 vial of Brilliance Candida Selective Supplement reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool the medium to 45°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Brilliance Candida Agar Base must be stored tightly capped in the original container at 10-30°C. When stored as directed the unopened products will remain stable until the expiry date printed on the containers.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Brilliance Candida is a chromogenic medium for the isolation and differentiation of Candida species.

• Add to to Brilliance Candida Agar Base, Part No. CM1002A and CM1002B
• Each vial supplements 500mL of medium

Brilliance Candida Selective Supplement, Oxoid Composition 

Brilliance Candida Agar, Oxoid Preparation:
Suspend 15.6g in 500ml of distilled water. Add 1 vial of Brilliance Candida Selective Supplement reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool the medium to 45°C and pour into sterile Petri dishes.

Storage conditions and Shelf life
Brilliance Candida Selective Supplement must be stored at 2-8°C. When stored as directed the unopened products will remain stable until the expiry date printed on the containers.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Differentiate and enumerate Cronobacter sakazakii from infant formula and other food samples with Thermo Scientific™ Brilliance™ Cronobacter Sakazakii Agar (Dehydrated). A chromogenic substrate present in the medium detects a-glucosidase reaction in Cronobacter sakazakii. The enzyme a-glucosidase, present in Cronobacter sakazakii, hydrolyses the substrate producing blue-green colonies on this pale yellow medium.

Brilliance Cronobacter Sakazakii Agar, Oxoid Composition

Brilliance Cronobacter Sakazakii Agar, Oxoid Preparation:
Suspend 43.1g of Brilliance Cronobacter sakazakii Agar in 1 litre of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Brilliance E. coli/Coliform Agar is a chromogenic medium for differentiation of Escherichia coli and other coliforms from food and environmental samples.

Brilliance E. coli/Coliform Agar, Oxoid Composition

Brilliance E. coli/Coliform Agar, Oxoid Preparation:
Suspend 55.8g of Brilliance E.coli/coliform agar in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour and into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C, and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Refer to the material safety data sheet before handling this product.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give purple colonies on this medium.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between Escherichia coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies

Brilliance E. coli/coliform Selective Agar, Oxoid Composition

Brilliance E. coli/coliform Selective Agar, Oxoid Preparation:
Suspend 28.1g of Brilliance E. coli/coliform Selective Agar in 1 litre of distilled water. Bring the medium gently to the boil, to dissolve completely. Either pour the medium into sterile Petri dishes or keep molten at 45ºC for pour plate technique.

Storage conditions and Shelf life
Dehydrated medium: store tightly capped in the original container at 10-30°C
Prepared medium will be stable for up to 2 weeks when stored at 2-8ºC

This product has been discontinued, please refer to SR0258E

Oxoid Brilliance Listeria Differential Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria

• Add to Brilliance Listeria Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Oxoid Brilliance Listeria Differential Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Brilliance Listeria Selective Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria spp. in food samples.

• Add to Brilliance Listeria Selective Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Brilliance Listeria Selective Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Brilliance Salmonella Agar Base is a chromogenic medium (formerly OSCM II) for isolation and presumptive identification of Salmonella spp. from food samples.

Brilliance Salmonella Agar Base, Oxoid Composition

 Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Brilliance Salmonella Agar should be stored in the tightly capped original container at 10-30ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Precautions
Brilliance Salmonella Agar is for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration.
Sterilise specimens, equipment and media properly after use.

Easily isolate, enumerate, and presumptively identify common organisms associated with urinary tract infections using Thermo Scientific™ Brilliance™ UTI Clarity™ Agar. Organisms are differentiated through the utilization of two chromogenic substrates and/or tryptophan.

Brilliance UTI Clarity Agar, Oxoid Composition

Brilliance UTI Clarity Agar, Oxoid Preparation:
To prepare the medium from the base, suspend 37g of Brilliance UTI Clarity Agar (CM1106) in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121ºC for 15 minutes. Cool the medium to 50ºC and pour into sterile Petri dishes.

Storage conditions and Stability
Dehydrated Brilliance UTI Clarity Agar must be stored tightly capped in the original container at 10-30ºC.

Oxoid Brilliance UTI Clarity Agar plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light.

When stored as directed, the unopened product will remain stable until the expiry date on the label. Locally prepared media can be stored for up to 2 weeks when made from CM1106 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
Brilliance UTI Clarity Agar is for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration.

It should be noted that, as with all chromogenic media, organisms with atypical enzyme patterns may give anomalous reactions. The medium should be validated with locally prevalent strains to confirm sensitivity and specificity in the end-users hands, under local conditions.

A presumptive E. coli identification can be confirmed using DMAC indole reagent (do not use Kovac’s reagent as the colour of the pink colonies may be mistaken for the red colour of a positive indole result). The test should be performed on filter paper, not directly on the plate.

Sterilise specimens, equipment and media properly after use.

This product has been discontinued, please refer to CM1212B
Brilliance Listeria Agar Base is a chromogenic medium for the isolation, enumeration, and presumptive identification of Listeria spp., including Listeria monocytogenes. Also available Chromogenic Listeria Selective Supplement, Part No. SR0227E and Chromogenic Listeria Differential Supplement, Part No. SR0228E.

Brilliance Listeria Agar Base, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid Brilliance™ UTI Agar (formerly Chomogenic UTI Agar ) is a chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections (UTIs).

Brilliance UTI Agar, Oxoid Composition

Brilliance UTI Agar, Oxoid Preparation:
Suspend 56.3g of Brilliance UTI Agar in 1 litre of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before pouring plates.

Storage conditions and Shelf life
Dehydrated Brilliance UTI Agar must be stored tightly capped in the original container at 10-30ºC.
Oxoid Brilliance UTI Agar plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light.

When stored as directed, the unopened product will remain stable until the expiry date on the label. Locally prepared media can be stored for up to 2 weeks when made from CM0949 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.