Microbiology

This product has been discontinued. The alternative product is Thioglycollate Fluid Medium USP (CM0173).

Oxoid Thioglycollate Medium, Brewer is an anaerobic medium especially useful for sterility control of solutions containing mercury-based preservatives.

Thioglycollate Medium, Brewer, Oxoid Composition

Thioglycollate Medium, Brewer, Oxoid Preparation:
Suspend 20g in 1 litre of distilled water. Bring to the boil, mix well and allow to stand until completely dissolved. Distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Media containing small quantities of agar are liable to separate if cooled rapidly. Tubes of reconstituted and autoclaved medium should be allowed to cool slowly on a wooden surface in a draught-free atmosphere.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at room temperature.

Precautions
Check the upper portion of the prepared medium before inoculation. If more than one-third of the fluid is oxidised i.e. green coloured, discard the bottle.

If one-third or less fluid is oxidised, then heat in boiling water with the cap loosened to drive off oxygen. Cool to room temperature before inoculation. This reheating process can only be carried out once because of the formation of toxic radicles in the medium.

Organisms which ferment glucose and lower the pH to critical levels may not survive in this medium after growth has taken place.

• Oxoid Thiosulphate Ringers Tablets is used for preparation of isotonic diluents for bacterial cells and bacteriological specimens.
• Each tablet prepares 500mL solution.

Thiosulphate Ringers Tablets, Oxoid Composition

* Due to the low ionic content of this medium no pH is specified.
One tablet makes 500ml of quarter-strength Ringer containing 0.05% of sodium thiosulphate.

Thiosulphate Ringers Tablets, Oxoid Preparation:
To prepare quarter-strength Ringer Solution containing thiosulphate, dissolve 1 tablet in 500ml distilled water. Sterilise by autoclaving at 121°C for 15 minutes.

Oxoid Tinsdale Agar Base is used for the isolation and identification of Corynebacterium diphtheriae.

Tinsdale Agar Base, Oxoid Composition

Tinsdale Agar, Oxoid Preparation:
Suspend 9g of agar base in 200ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to approximately 50°C and add the contents of one vial of Oxoid Tinsdale Supplement SR0065 reconstiuted as directed. Mix throughly and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 4 days.

Precautions
Further tests must be carried out on colonies suspected as Corynebacterium diphtheriae, including cell morphology after sub-culture to Loeffler’s Medium and examination for toxin production.
Do not incubate Tinsdale’s Agar plates in enhanced CO2 atmosphere (5-10% v/v).

This product has been discontinued. Please contact us for more information.

Oxoid Tinsdale Supplement is used for the cultivation of Corynebacterium diphtheriae.

• A supplement for Tinsdale Medium, Part No. CM0487B
• Each vial supplements 200mL of medium

Tinsdale Supplement, Oxoid Composition

Tinsdale Agar, Oxoid Preparation:
Suspend 9g of agar base in 200ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to approximately 50°C and add the contents of one vial of Oxoid Tinsdale Supplement SR0065 reconstiuted as directed. Mix throughly and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 4 days.

Precautions
Further tests must be carried out on colonies suspected as Corynebacterium diphtheriae, including cell morphology after sub-culture to Loeffler’s Medium and examination for toxin production.
Do not incubate Tinsdale’s Agar plates in enhanced CO2 atmosphere (5-10% v/v).

Oxoid Todd-Hewitt Broth is used for cultivation of Streptococci prior to serological grouping.

Todd-Hewitt Broth, Oxoid Composition

Todd-Hewitt Broth, Oxoid Preparation:
Dissolve 36.4 g in 1 litre of distilled water. Mix well, distribute into containers and sterilise by autoclaving at 115°C for 10 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Streptococcus species grown in Todd-Hewitt Broth and harvested as antigens to raise antibodies, will sometimes carry antigenic material from the broth. This problem must be looked for in the antisera. If detected it would be preferable to use another medium to grow the test streptococci.

**This product is not available at the moment. Please contact us for more information.

Oxoid Tomato Juice Agar is used for cultivation of lactobacilli.

Tomato Juice Agar, Oxoid Composition

Tomato Juice Agar, Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the plates of prepared medium at 2-8°C.

Oxoid Trichomonas Medium is used for cultivation of Trichomonas vaginalis.

Trichomonas Medium, Oxoid Composition

Trichomonas Medium, Oxoid Preparation:
Suspend 37.5g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Inactivate 80ml of horse serum, adjust to pH 6.0 and add it to the medium. For diagnostic work, bacterial growth may be suppressed by the addition of 1000 units of penicillin and 500µg of streptomycin per ml of medium or 100µg of chloramphenicol per ml of medium (1 vial of SR0078 per 500ml medium).

Prior to incorporation in the medium, Horse Serum SR0035 is inactivated by maintaining at a temperature of 56°C for 30 minutes, and then acidified with 1N hydrochloric acid to pH 6.0.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Differentiate Enterobacteriaceae according to their ability to ferment lactose, sucrose, and glucose, and to produce hydrogen sulphide using Thermo Scientific™ Oxoid™ Triple Sugar Iron Agar (Dehydrated). The Triple Sugar Iron Agar is based on Kligler Iron Agar, additionally, it contains sucrose for the detection of sucrose fermenting species.

Triple Sugar Iron Agar, Oxoid Composition

Triple Sugar Iron Agar, Oxoid Preparation:
Suspend 65g in 1 litre of distilled water. Bring to the boil to dissolve completely. Mix well and distribute. Sterilise by autoclaving at 121°C for 15 minutes. Allow the medium to set in sloped form with a butt about 1 in.deep.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

The Thermo Scientific™ Oxoid Tryptone ia a pancreatic digest of casein for use where high performance and uniformity of composition are of paramount importance.

The Thermo Scientific™ Oxoid Tryptone ia a pancreatic digest of casein for use where high performance and uniformity of composition are of paramount importance.

The Oxoid Tryptone Bile Agar is used for the detection and enumeration of E.coli biotype 1 in foods.

Tryptone Bile Agar, Oxoid Composition

Tryptone Bile Agar, Oxoid Preparation:
Suspend 36.5g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour 12-15ml of the medium into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Tryptone Glucose Extract Agar is a standard method medium for examination of water and dairy products.

Tryptone Glucose Extract Agar, Oxoid Composition

Tryptone Glucose Extract Agar, Oxoid Preparation:
Suspend 24 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. When the dilution of the original specimen is greater than 1 in 10, add 10 ml of sterile 10% solution of Skim Milk Powder LP0031 per litre.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Grow a wide variety of microorganisms with Thermo Scientific ™ Oxoid ™ Tryptone Soya Agar EP/USP/JP/BP (Dehydrated). A general purpose agar medium containing two peptones, supports the growth of both aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. Tryptone Soya Agar is recommended as a reference medium when testing selective media to measure the degree of inhibition.

*Contains papain

Preparation of Tryptone Soya Agar, Oxoid

First, you add 40g of tryptone soya agar to 1 litre of distilled water (purified as required). You can boil to dissolve the powder completely. After dissolved, you can sterilise the medium by autoclaving at 121°C for 15 minutes.

Storage Condition and Shelf Life of Tryptone Soya Agar, Oxoid

You can store the dehydrated tryptone soya agar at 10-30°C and use before the expiry date on the label or store the prepared plates of a medium at 2-8°C.

Tryptone Soya Agar, Oxoid (dehydrated) is suitable to grow a wide variety of microorganisms. It’s a general-purpose agar medium containing two peptones, which supports the growth of both aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. Tryptone Soya Agar is recommended as a reference medium when testing selective media to measure the degree of inhibition.

*Contains papain

Preparation of Tryptone Soya Agar, Oxoid

First, you add 40g of tryptone soya agar to 1 litre of distilled water (purified as required). You can boil to dissolve the powder completely. After dissolved, you can sterilise the medium by autoclaving at 121°C for 15 minutes.

Storage Condition and Shelf Life of Tryptone Soya Agar, Oxoid

You can store the dehydrated tryptone soya agar at 10-30°C and use before the expiry date on the label or store the prepared plates of a medium at 2-8°C.

Thermo Scientific™ Oxoid Tryptone Soya Broth is tested according to EP/USP.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

Grow a wide variety of microorganisms with Thermo Scientific™ Oxoid™ Tryptone Soya Broth (a.k.a Tryptic Soy Broth) EP/USP/BP/JP (Dehydrated). The general purpose medium, containing two peptones, is suitable for the cultivation of both aerobes and anaerobes. It is specified in International Pharmacopoeia and food testing methods for the growth of a wide variety of organisms.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.