Microbiology

Cultivate and isolate fastidious microorganisms and determine their hemolytic reactions using Thermo Scientific™ Oxoid™ Tryptose Blood Agar Base (Dehydrated) with supplementation of blood. This highly nutritious medium is specially developed by Casman for the preparation of a blood agar which will support the growth of many fastidious organisms.

The original formulation included 0.3g dextrose per litre which interfered with the hemolytic reactions. It is now used without dextrose as a standard medium.

Tryptose Blood Agar Base (Dehydrated), Oxoid Composition

Tryptose Blood Agar Preparation:
Suspend 30g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

For blood agar, cool the basal medium to 45-50°C and add 7% of sterile blood. Mix thoroughly, taking care to avoid incorporation of air bubbles, and dispense into Petri dishes or other containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

An improved medium, based on the Hynes modification of Leifsons medium for the isolation of Salmonellae and Shigellae. The improvement gives larger and more numerous colonies of Shigella species which can easily be picked off and emulsified in saline for slide agglutination tests.

Desoxycholate Citrate Agar (Hynes), Oxoid Composition

Desoxycholate Citrate Agar (Hynes), Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Bring to the boil over gauze and flame, to dissolve completely. Agitate to prevent charring. Cool to approximately 50°C and pour into sterile Petri dishes. Dry the agar surface before use.
THIS MEDIUM IS HEAT SENSITIVE: AVOID EXCESSIVE OR PROLONGED HEATING DURING RECONSTITUTION. DO NOT AUTOCLAVE OR REMELT.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
The medium is best used freshly prepared.
Stock cultures of Shigella species may become predominantly in the R-phase when subcultured away from DCA media. Such cultures are difficult to use for control purposes without first heavily streaking the cultures on DCA plates and picking off the few S-phase colonies i.e. the macro-colonies on the agar surface, for further subculture.
When making biochemical tests on colonies picked from the surface of DCA plates, purity subcultures should be carried out because the colony may be contaminated with Escherichia coli present as micro-colonies.

A simple medium originally described by Crossley for the routine examination of canned food samples for anaerobic bacteria, this medium has evolved as the result of comparative trials carried out by Crossley with several standard media. It is capable of giving rapid growth without the use of special anaerobic apparatus, yet the bacteria detected may be provisionally identified by their reactions upon the medium.

Crossley milk medium, Oxoid Composition

Crossley milk medium, Oxoid Preparation:
Cream 110g of the powder with a little distilled water and gradually dilute to 1 litre with continuous mixing. Tube in 10ml quantities and autoclave at 121°C for 5 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Staphylococcus Medium No.110 is a selective medium is used for isolation and differentiation of pathogenic Staphylococci.

Staphylococcus Medium No.110, Oxoid Composition

Staphylococcus Medium No.110, Oxoid Preparation:
Suspend 150g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Disperse the precipitate by gentle agitation before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Enterococcus faecalis may grow on this medium as tiny colonies with slight mannitol fermentation.
The high salt content in Staphylococcus Medium No.110 may interfere with the coagulase reaction. Always sub-culture to a non-inhibitory medium before testing.

**This product is not available at the moment. Please contact us for more information.

Oxoid Tomato Juice Agar is used for cultivation of lactobacilli.

Tomato Juice Agar, Oxoid Composition

Tomato Juice Agar, Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the plates of prepared medium at 2-8°C.

Oxoid MacConkey Agar No. 2 is a modification of MacConkey Agar No. 3 with addition of Bile Salts No. 2 for detection of Enterococci.

MacConkey Agar No. 2, Oxoid Composition

MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Enrich and isolate halophilic organisms, especially staphylococci, using Thermo Scientific™ Oxoid™ Salt Meat Broth Tablets. Salt Meat Broth Tablets are specially designed for the isolation of staphylococci from grossly contaminated specimens such as feces, particularly during the investigation of staphylococcal food poisoning. The medium is highly sensitive as it can detect even low numbers of staphylococci from samples having large proportions of heterogeneous microbial flora.^1,2

• Quantity: 100 tablets/bottle

Salt Meat Broth Tablets, Oxoid Composition

Salt Meat Broth, Oxoid Preparation:
Add 2 tablets to 10ml of distilled water in an appropriately sized test tube and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

This product has been discontinued. The alternative to this product is Peptone Water.

Oxoid Peptone Water Andrade’s is a nutrient base containing Andrade’s indicator to which carbohydrates and other diagnostic reagents may be added for use in fermentation studies.

Peptone Water Andrade’s, Oxoid Composition

Peptone Water Andrade’s, Oxoid Preparation:
Suspend 15 g in 1 litre of distilled water. Mix well to dissolve, distribute into tubes or bottles, containing Durham tubes and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium in the dark at room temperature.

This product has been discontinued. The alternative product is Thioglycollate Fluid Medium USP (CM0173).

Oxoid Thioglycollate Medium, Brewer is an anaerobic medium especially useful for sterility control of solutions containing mercury-based preservatives.

Thioglycollate Medium, Brewer, Oxoid Composition

Thioglycollate Medium, Brewer, Oxoid Preparation:
Suspend 20g in 1 litre of distilled water. Bring to the boil, mix well and allow to stand until completely dissolved. Distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Media containing small quantities of agar are liable to separate if cooled rapidly. Tubes of reconstituted and autoclaved medium should be allowed to cool slowly on a wooden surface in a draught-free atmosphere.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at room temperature.

Precautions
Check the upper portion of the prepared medium before inoculation. If more than one-third of the fluid is oxidised i.e. green coloured, discard the bottle.

If one-third or less fluid is oxidised, then heat in boiling water with the cap loosened to drive off oxygen. Cool to room temperature before inoculation. This reheating process can only be carried out once because of the formation of toxic radicles in the medium.

Organisms which ferment glucose and lower the pH to critical levels may not survive in this medium after growth has taken place.

Oxoid Lab-Lemco Broth is a nutrient medium equivalent to USA nutrient extract formulations.

Lab-Lemco Broth, Oxoid Composition

Lab-Lemco Broth, Oxoid Preparation:
Dissolve 8g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.
`Lab-Lemco’ Broth is slightly more alkaline than the medium used in the APHA publications (pH 6.8).

Oxoid Nutrient Agar Tablets make 5mL of medium each.

Nutrient Agar Tablets, Oxoid Composition

Nutrient Agar, Oxoid Preparation:
Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid MUG Supplement (50mg) Fluorescent agent is used for the detection of E. coli.

• Contains 4-methylumbelliferyl-β-D-glucuronide (MUG)
• Each vial supplements 500mL of medium

M.U.G. Supplement, Oxoid Composition

M.U.G. Supplement, Oxoid Preparation:

Add 2ml of distilled water to a vial and invert gently until completely dissolved. Add the vial contents to the following volumes of suggested media, before sterilisation.

Storage conditions and Shelf life
Should be stored at 2-8°C. When stored as directed the unopened vial is stable until the expiry date on the label.

Precautions
The presence of endogenous glucuronidase in shellfish samples may result in false positive fluorescence. Test tubes used in the MPN method should be checked under UV light to ensure the glass does not fluoresce. To avoid false positive fluorescence the source of long wave UV light must not exceed 6 watts.

This product is temporarily unavailable. Please contact us for more information.

Oxoid Saline Tablets is used for preparation of 500mL of 0.85% isotonic saline. For Laboratory Use Only.

Oxoid Ringers Solution Tablets is used for the preparation of isotonic diluents for bacterial cells and bacteriological specimens.

Each tablet prepares 500mL solution. For Laboratory Use Only.

Ringers Solution Tablets, Oxoid Composition

One tablet makes 500 ml of quarter-strength Ringers solution.

Ringers Solution Preparation:
To prepare quarter-strength Ringers Solution, dissolve 1 tablet in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes.

Oxoid Basic Fuchsin Indicator is a dye for use with Endo Agar Base, Part No. CM0479B and m Endo (LES) Agar Base, Part No. MM0551B.

Basic Fuchsin Preparation:

BR0050 should be dissolved to create a 10% w/v solution using 95% ethyl alcohol.

For each litre of culture medium use the amount described in the product reconstitution details.  This should be in a 10% w/v solution of the dye dissolved in 50:50 ethanol/distilled water.  E.g. For CM0479 add 6ml of a 10% w/v Basic Fuchsin solution per litre of medium.

The magenta dyes are closely related to known carcinogenic substances; therefore basic fuchsin should be handled with care, avoiding inhaling the powder or staining the skin with dye.

• Oxoid Thiosulphate Ringers Tablets is used for preparation of isotonic diluents for bacterial cells and bacteriological specimens.
• Each tablet prepares 500mL solution.

Thiosulphate Ringers Tablets, Oxoid Composition

* Due to the low ionic content of this medium no pH is specified.
One tablet makes 500ml of quarter-strength Ringer containing 0.05% of sodium thiosulphate.

Thiosulphate Ringers Tablets, Oxoid Preparation:
To prepare quarter-strength Ringer Solution containing thiosulphate, dissolve 1 tablet in 500ml distilled water. Sterilise by autoclaving at 121°C for 15 minutes.