Microbiology

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated) while adhering to ISO 21527-1: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.

Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated), Oxoid Composition

Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Preparation:
Suspend 15.75g per 500ml (31.5g/l) of distilled water and heat to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes. It is advised to dispense the powdered medium into containers under an extraction hood due to the chloramphenicol in the medium base.

Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date

Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Pre-supplemented DRBC (ISO) Agar (CM1149) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.

Easily isolate, enumerate, and presumptively identify common organisms associated with urinary tract infections using Thermo Scientific™ Brilliance™ UTI Clarity™ Agar. Organisms are differentiated through the utilization of two chromogenic substrates and/or tryptophan.

Brilliance UTI Clarity Agar, Oxoid Composition

Brilliance UTI Clarity Agar, Oxoid Preparation:
To prepare the medium from the base, suspend 37g of Brilliance UTI Clarity Agar (CM1106) in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121ºC for 15 minutes. Cool the medium to 50ºC and pour into sterile Petri dishes.

Storage conditions and Stability
Dehydrated Brilliance UTI Clarity Agar must be stored tightly capped in the original container at 10-30ºC.

Oxoid Brilliance UTI Clarity Agar plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light.

When stored as directed, the unopened product will remain stable until the expiry date on the label. Locally prepared media can be stored for up to 2 weeks when made from CM1106 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
Brilliance UTI Clarity Agar is for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration.

It should be noted that, as with all chromogenic media, organisms with atypical enzyme patterns may give anomalous reactions. The medium should be validated with locally prevalent strains to confirm sensitivity and specificity in the end-users hands, under local conditions.

A presumptive E. coli identification can be confirmed using DMAC indole reagent (do not use Kovac’s reagent as the colour of the pink colonies may be mistaken for the red colour of a positive indole result). The test should be performed on filter paper, not directly on the plate.

Sterilise specimens, equipment and media properly after use.

Enrich and recover Salmonella spp. from food samples in a single step with Thermo Scientific™ Oxoid™ ONE Broth-Salmonella Base, when prepared with Thermo Scientific Oxoid ONE Broth-Salmonella Selective Supplement (SR0242).

ONE Broth-Salmonella Base, Oxoid Composition

ONE Broth-Salmonella Base, Oxoid Preparation:
Suspend 5.6g of ONE Broth-Salmonella Base in 225ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to below 50°C and add the contents of 1 vial of ONE Broth-Salmonella Supplement (SR0242), resuspended as directed.

Storage conditions and Shelf life
ONE Broth-Salmonella should be stored tightly capped in the original container at 10-30ºC. When stored as directed, the unopened products will remain stable until the expiry date printed on the packaging.

Once prepared, ONE Broth-Salmonella can be stored for up to 2 weeks when made from CM1091 and SR0242 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
ONE Broth-Salmonella medium and Brilliance Salmonella Agar are for in vitro diagnostic use only, by experienced microbiologists. It must not be used beyond the stated expiry date, or if the product shows any sign of deterioration. Sterilize specimens, equipment and media properly after use; dispose waste in accordance with local rules and regulations. Directions should be read and followed carefully.

This product has been discontinued, please refer to CM1212B

Isolate, enumerate and differentiate Listeria species and Listeria monocytogenes from food samples with Thermo Scientific™ Oxoid™ Chromogenic Listeria Agar (ISO) (Dehydrated). Chromogenic Listeria Agar (ISO) contains lithium chloride, polymyxin B, nalidixic acid, and amphotericin which act to inhibit non-target organisms. A combination of chromogenic and phospholipase enzyme reactions easily differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).

Chromogenic Listeria Agar (ISO), Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to CM1212B
Brilliance Listeria Agar Base is a chromogenic medium for the isolation, enumeration, and presumptive identification of Listeria spp., including Listeria monocytogenes. Also available Chromogenic Listeria Selective Supplement, Part No. SR0227E and Chromogenic Listeria Differential Supplement, Part No. SR0228E.

Brilliance Listeria Agar Base, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance™ E. coli / Coliform Selective Agar. The agar base uses two chromogens to differentiate between E. coli and other coliforms. This results in purple E. coli, as they are able to cleave both chromogens while other coliforms, giving pink colonies.

ONE Broth-Listeria Base, Oxoid Composition

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Base must be stored tightly capped in the original container at 10-30°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

Precautions
ONE Broth-Listeria Base and ONE Broth Selective Supplement are for Laboratory Use Only.
ONE Broth-Listeria Base is designated as “Irritant” and ONE Broth-Listeria Selective Supplement is designated as “Harmful”. For a full Material Safety Data Sheet (MSDS).
Do not use ONE Broth-Listeria Base or ONE Broth-Listeria Selective Supplement beyond the stated expiry dates, or if the products show any sign of deterioration.

Differentiate and enumerate Cronobacter sakazakii from infant formula and other food samples with Thermo Scientific™ Brilliance™ Cronobacter Sakazakii Agar (Dehydrated). A chromogenic substrate present in the medium detects a-glucosidase reaction in Cronobacter sakazakii. The enzyme a-glucosidase, present in Cronobacter sakazakii, hydrolyses the substrate producing blue-green colonies on this pale yellow medium.

Brilliance Cronobacter Sakazakii Agar, Oxoid Composition

Brilliance Cronobacter Sakazakii Agar, Oxoid Preparation:
Suspend 43.1g of Brilliance Cronobacter sakazakii Agar in 1 litre of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Numerate all culturable microorganisms from water using colony count technique with Thermo Scientific™ Oxoid™ Water Plate Count Agar (Dehydrated). This nutritious, non-selective medium conforms to ISO 6222: 1999 (E) Water quality ― Enumeration of culturable microorganisms ― Colony count by inoculation in a nutrient agar culture medium1.

Water Plate Count Agar (Dehydrated), Oxoid Composition

Water Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 24g of Water Plate Count Agar (ISO) in 1 litre of distilled water and bring gently to the boil to dissolve. If desired, distribute volumes of 15ml to 20ml in tubes, bottles or other containers. Sterilise by autoclaving at 121°C for 15 minutes. After sterilisation, allow to cool and maintain at 45 ± 1°C using a water bath.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

• Oxoid Oxacillin Resistance Screening Agar Base (ORSAB) is a medium for screening clinical samples for the presence of oxacillin-resistant Staphylococci.
• Also available ORSAB Selective Supplement, Part No. SR0195E.

Oxacillin Resistance Screening Agar Base (ORSAB), Oxoid Composition

Oxacillin Resistance Screening Agar, Oxoid Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Cefixime Rhamnose Sorbitol MacConkey Agar Base (CR-SMAC) is a selective and differential medium containing rhamnose and cefixime for the isolation of E. coli O157.
• Also available Cefixime Supplement, Part No. SR0191E.

CR-SMAC Agar Base, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples.
• Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.

Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition

(M-CP) Agar Base, Oxoid Preparation:

Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to 5 days at 2-8°C in the dark.

Oxoid VRE Agar Base (Vancomycin Resistant Enterococci Agar Base) is used for selective isolation of vancomycin-resistant enterococci and high level aminoglycoside-resistant enterococci (HLARE) from clinical samples.Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Agar Base, Oxoid Composition

VRE Agar, Oxoid Preparation:
Suspend 42.6g of VRE Agar Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121° C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then Mix agar well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.
Warning: Sodium azide is harmful if swallowed, please take all necessary precautions.

This product has been discontinued. Please contact us for more information.

• Oxoid VRE Broth Base (Vancomycin Resistant Enterococci Broth Base) is for selective isolation of vancomycin-resistant enterococci and high Level aminoglycoside-resistant enterococci.
• Also available Meropenem Selective Supplement, Part No. SR0184E; Gentamicin Selective Supplement, Part No. SR0185E; and Vancomycin Selective Supplement, Part No. SR0186E.

VRE Broth Base, Oxoid Composition

VRE Broth, Oxoid Preparation:
Suspend 37.0g of VRE Broth Base in 1 litre of distilled water. Warm to dissolve completely, sterilise by autoclaving at 121°C for 15 minutes and cool to 50°C. Supplement the medium as shown in the table below. Then mix well and distribute into final sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Precautions
VRE Broth Base, VRE Agar Base , Meropenem Supplement SR0184, Gentamicin Supplement SR0185 and Vancomycin Supplement SR0186 should only be used for in vitro diagnostic purposes. Do not use any of these beyond expiry date or if the products show any signs of deterioration.

Oxoid Lauryl Tryptose Broth w/MUG is used for the presumptive identification of E. coli.

Lauryl Tryptose Broth with MUG, Oxoid Composition

Lauryl Tryptose Broth, Oxoid Preparation:
Suspend 35.65g of Lauryl Tryptose Broth with MUG in 1 litre of distilled water. Warm to 40°C. Mix well to dissolve and distribute into final containers holding inverted Durham tubes. (Please be aware of the special glassware requirements when using ultra-violet light.). Sterilise by autoclaving at 121° C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

• Oxoid Arcobacter Broth Base is used for the selective growth of Arcobacter spp.
• Also available CAT Supplement, Part No. SR0174E and CCDA Selective Supplement, Part No. SR0155E and SR0155H.

Arcobacter Broth Base, Oxoid Composition

Arcobacter Broth Base, Oxoid Preparation:

Dissolve 12g of Arcobacter Broth in 500ml of distilled water. Sterilise at 121°C for 15 minutes and allow to cool to below 50°C

For use with CAT supplement (SR0174)

Add one vial per 500ml medium of CAT Selective Supplement reconstituted as directed. Dispense into sterile containers.

For use with CCDA supplement (SR0155)

Add one vial per 500ml medium of CCDA Selective Supplement reconstituted as directed. Dispense into sterile containers.

Inoculated plates should be incubated aerobically, at 30°C, for 24 hours.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Brilliance E. coli/Coliform Agar is a chromogenic medium for differentiation of Escherichia coli and other coliforms from food and environmental samples.

Brilliance E. coli/Coliform Agar, Oxoid Composition

Brilliance E. coli/Coliform Agar, Oxoid Preparation:
Suspend 55.8g of Brilliance E.coli/coliform agar in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour and into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C, and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Refer to the material safety data sheet before handling this product.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give purple colonies on this medium.

Oxoid Azide Dextrose Broth (Rothe Broth) is used for the detection of enterococciin water, sewage, and food.

Azide Dextrose Broth (Rothe Broth), Oxoid Composition

Azide Dextrose Broth (Rothe Broth), Oxoid Preparation:
Add 35.6g to one litre of distilled water for single strength broth or 71.2g for double strength broth. Heat gently to dissolve. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
This product contains less than 1% azide and has low toxicity. However, when handling the powder, wear gloves, mask and eye protection. When washing azide products down sinks, use sufficient water to prevent accumulation of azide in the plumbing.

Oxoid Salmonella Rapid Test Elective Medium (SRTEM) is a selective enrichment broth medium specifically formulated for use with the Oxoid Salmonella Rapid Test Kit, Part No. FT0201A.

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Composition

Salmonella Rapid Test Elective Medium (SRTEM), Oxoid Preparation:
Add 61g to 1 litre of distilled water (double strength medium). Sterilize by autoclaving at 121°C for 15 minutes. Cool to 18-25°C and use as described in the Oxoid Salmonella Rapid Test (Code FT0201) instruction leaflet.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.