Microbiology

Oxoid Hemoglobin Powder Soluble is a specially prepared hemoglobin for use in culture media.

• Oxoid STAA Agar Base is used for the isolation of Brochothrix thermosphactafrom food samples.
• Also available STAA Selective Supplement, Part No. SR0151E and STA Selective Supplement, Part No. SR0162E.

STAA Agar Base, Oxoid Composition

STAA Agar, Oxoid Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add:

a) for STAA Medium the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

b) for STA Medium the contents of 1 vial of STA Selective Supplement SR0162 reconstituted as directed.
Mix well and distribute into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Detect thermophilic anaerobic organisms causing sulphide spoilage in food with Thermo Scientific™ Oxoid™ Iron Sulphite Agar (Dehydrated). The medium is a modification of Cameron Sulphite Agar developed by the National Canners Association of America1. Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes.

Iron Sulphite Agar, Oxoid Composition

Iron Sulphite Agar, Oxoid Preparation:
Suspend 23.0g in 1 litre of distilled water and boil to dissolve completely. Sterilise by autoclaving for 15 minutes at 121°C. Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Precautions
The blackening reaction is only presumptive evidence of clostridial growth. Confirmation tests must be carried out to identify the organism growing in the medium.

Thermo Scientific™ Oxoid T.T.C. Solution is a solution for the addition to KF Streptococcus Agar for the enumeration of entercocci.

T.T.C. Solution, Oxoid Composition

Medium Preparation:
Aseptically add the contents to 500ml of KF Streptococcus Agar CM0701 cooled to approximatly 50°C. Mix gently and pour into sterile Petri dishes.

• Oxoid Campylobacter Selective Supplement, Skirrow is used for preparation of Skirrow Selective Medium for the isolation of Campylobacter spp. at 42°C.
• Add to Blood Agar Base No. 2, Part No. CM0271B or Columbia Blood Agar Base, Part No. CM0331B.

Campylobacter Selective Supplement, Skirrow, Oxoid Composition

Campylobacter Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed aseptically add the contents to 500 ml of a nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2 , with 10% defibrinated horse or sheep blood SR0050,SR0051 or 5-7% laked horse blood SR0048. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Todd-Hewitt Broth is used for cultivation of Streptococci prior to serological grouping.

Todd-Hewitt Broth, Oxoid Composition

Todd-Hewitt Broth, Oxoid Preparation:
Dissolve 36.4 g in 1 litre of distilled water. Mix well, distribute into containers and sterilise by autoclaving at 115°C for 10 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Streptococcus species grown in Todd-Hewitt Broth and harvested as antigens to raise antibodies, will sometimes carry antigenic material from the broth. This problem must be looked for in the antisera. If detected it would be preferable to use another medium to grow the test streptococci.

Oxoid Dextrose Tryptone Agar is used for detection and enumeration of thermophilic and mesophilic microorganisms in food.

Dextrose Tryptone Agar, Oxoid Composition

Dextrose Tryptone Agar, Oxoid Preparation:
Suspend 27g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
Incubation at 55°C must be carried out under humid conditions e.g. wrapped dishes or in a high humidity environment.

M17 Agar, Oxoid is used for the improved growth of Lactic Streptococci and their bacteriophages and the selective enumeration of Streptococcus thermophilus from yogurt.

M17 Agar, Oxoid Composition

M17 Agar, Oxoid Preparation:
Suspend 48.25g in 950ml of distilled water and bring gently to the boil. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2µm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Isolate and enumerate microorganisms in milk and dairy products with Thermo Scientific™ Oxoid™ Milk Plate Count Agar (Dehydrated). Milk Plate Count Agar is equivalent to the medium recommended by British Standard Institution and International Organization for Standardization.

Milk Plate Count Agar (Dehydrated), Oxoid Composition

Milk Plate Count Agar (Dehydrated), Oxoid Preparation:
Suspend 19.5g in 1 litre of distilled water. Bring to the boil with frequent agitation, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Oxoid Modified Karmali Selective Supplement is for a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42° C.

• Alternative supplement containing amphotericin B as a replacement for cycloheximide
• Add to Campylobacter Agar Base (Karmali), Part No. CM0935B
• Each vial supplements 500mL of medium

Modified Karmali Selective Supplement, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Campylobacter Agar Base (Karmali) CM0935 cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

• Oxoid Anaerobe Basal Agar is used for growth of fastidious and other anaerobes.
• Also available N-S Anaerobe Selective Supplement, Part No. SR0107B, for use for nonsporforming anaerobes and G-N Anaerobe Selective Supplement, Part No. SR0108B, for use for gram-negative anaerobes.

Anaerobe Basal Agar, Oxoid Composition

Anaerobe Basal Agar, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium for upto 3 weeks at 2-8°C.

Oxoid Dextrose Tryptone Broth is used for detection and enumeration of thermophilic and mesophilic microorganisms in food.

Dextrose Tryptone Broth, Oxoid Composition

Dextrose Tryptone Broth, Oxoid Preparation:
Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared broth below 25°C.

Identify members of the coliform group of bacteria in food and water samples with Thermo Scientific™ Oxoid™ Minerals Modified Glutamate Medium (Dehydrated). The mineral content of the medium allows for the superior cultivation and enumeration of coliforms.

Minerals Modified Glutamate Medium, Oxoid Composition

Minerals Modified Glutamate Medium, Oxoid Preparation:
Double Strength:
Dissolve 5g of ammonium chloride in 1 litre of distilled water. To this add 22.7g of Minerals Modified Medium Base, and 12.7g of Sodium glutamate LP0124. Mix to dissolve completely. Sterilise by autoclaving for 10 minutes at 116°C; alternatively heat to 100°C for 30 minutes on three successive days.

Single strength:
Dissolve 2.5g of ammonium chloride in 1 litre of distilled water. To this add 11.4g of Minerals Modified Medium Base, and 6.4g of Sodium glutamate LP0124. Mix to dissolve completely. Sterilise by autoclaving for 10 minutes at 116°C; alternatively heat to 100°C for 30 minutes on three successive days.

Note
To improve the stability of the dehydrated medium on storage the sodium glutamate LP0124 is supplied separately and must be added to the basal medium CM0607.

The pH of the final medium is critical for optimum performance and the sterilised broth should be checked to confirm that it is at pH 6.7 before use.

Differences in heating procedures cause differences in final pH value. If necessary the heating procedure should be adjusted so that the final pH, after sterilisation is 6.7

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Presumptive positive tubes must be sub-cultured to Lauryl Tryptose Mannitol Broth CM0831 and incubated at 44°C to detect indole formation at this temperature before the identification of Escherichia coli can be made.

• Oxoid Dermasel Agar Base is used for primary isolation and identification of dermatophyte fungi.
• Also available Dermasel Selective Supplement, Part No. SR0075E.

Dermasel Agar Base, Oxoid Composition

Dermasel Agar, Oxoid Preparation:
Suspend 44.5g in 1 litre of distilled water and heat gently to dissolve completely. Add the contents of 1 vial of Dermasel Selective Supplement SR0075, reconstituted as directed in the product insert, to each 500ml of medium to give a level of cycloheximide 0.4g/l and chloramphenicol 0.05g/l. Mix gently and sterilise by autoclaving at 121°C for 10 minutes. Avoid overheating at any time.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
This medium should not be used if agents causing systemic mycoses are being sought⁹. If such agents are suspected e.g. cryptococcus, histoplasma etc., either Dermasel Agar Base without antibiotic supplement must be used in parallel or Brain Heart Infusion Agar CM0375.
If the fungal agent sought is suspected to be nutritionally fastidious, the use of Brain Heart Infusion Agar is particularly helpful.

• Oxoid Hoyle Medium Base is used for isolation and differentiation of Corynebacterium diphtheriae Types.
• Requires addition of Potassium Tellurite 3.5%, Part No. SR0030J

Hoyle Medium Base, Oxoid Composition

Hoyle Medium, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 55°C and add 50ml of Laked Horse Blood SR0048 and 10ml of 3.5% Potassium Tellurite solution SR0030, mix, and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
It should be noted that not all corynebacteria produce the typical colonies described above – so in all cases it is advisable to use Hoyle medium in conjunction with the additional media and tests mentioned above.

Cultivate lactic streptococci and their bacteriophages with Thermo Scientific™ Oxoid™ M17 Broth (Dehydrated). The use of M17 Broth in conjunction with M17 Agar is recommended by Terzaghi and Sandine for bacteriophage assays.

M17 Broth, Oxoid Composition

M17 Broth, Oxoid Preparation:
Suspend 37.25g in 950ml of distilled water and bring gently to the boil. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2mm membrane.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Heart Infusion Broth is a highly nutritious liquid medium recommended for the cultivation of fastidious microorganisms.

Heart Infusion Broth, Oxoid Composition

Heart Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121ºC for 15 minutes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Store the prepared medium in the dark below 20°C.

Precautions
Heart Infusion Broth should only be used for in vitro diagnostic purposes.

Isolate Campylobacter jejuni, Campylobacter coli and Compylobacter laridis using Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated) when used with CCDA Selective Supplement SR0155. Campylobacter Blood-Free Selective Agar Base is based on the original formulation described by Bolton et.al which was developed to replace blood with charcoal, ferrous sulphate and sodium pyruvate. For improved selectivity cephazolin in the formulation was replaced by cefoperazone as a selective agent.

Campylobacter Blood-Free Selective Agar Base, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

• Oxoid PALCAM Agar Base is a selective and diagnostic medium for the detection of Listeria monocytogenes.
• Also available PALCAM Selective Supplement, Part No. SR0150E or SR0150B.

PALCAM Agar Base, Oxoid Composition

PALCAM Agar, Oxoid Preparation:
Suspend 34.5g per 500ml of distilled water. Bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, and aseptically add PALCAM Selective Supplement (SR0150), reconstitutes as directed. Mix well and pour into sterile Petri dishes.

The addition of 2.5% (v/v) Egg Yolk Emulsion (SR0047) to the medium may aid the recovery of damaged Listeria.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 4 weeks at 2-8°C in the dark.

Precautions
Acriflavine hydrochloride is activated by light which may cause it to become inhibitory to Listeria growth.

Oxoid Modified Brucella Selective Supplement is for the cultivation and isolation of Brucella spp.

• Alternative supplement containing natamycin as a replacement for cycloheximide
• Add to Blood Agar Base No. 2, Part No. CM0271B or Brucella Medium Base, Part No. CM0169B
• Each vial supplements 500mL of medium

Modified Brucella Selective Supplement, Oxoid Composition

Modified Brucella Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml Brucella Medium Base, Columbia Agar Base or Blood Agar Base No.2 together with 5-10% v/v inactivated horse serum SR0035 and 1-5% w/v sterile solution of glucose, cooled to approximately 50°C. Mix well and pour into sterile Petri dishes.