Microbiology

Oxoid Tryptone Glucose Extract Agar is a standard method medium for examination of water and dairy products.

Tryptone Glucose Extract Agar, Oxoid Composition

Tryptone Glucose Extract Agar, Oxoid Preparation:
Suspend 24 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. When the dilution of the original specimen is greater than 1 in 10, add 10 ml of sterile 10% solution of Skim Milk Powder LP0031 per litre.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Milk Agar, Oxoid is for use in the dairy industry.

Milk Agar, Oxoid Composition

Milk Agar, Oxoid Preparation:
Suspend 24g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Wilkins-Chalgren Anaerobe Agar is used for the growth of anaerobes and recommended for antimicrobial susceptibility testing of anaerobes.

Wilkins-Chalgren Anaerobe Agar, Oxoid Composition

Wilkins-Chalgren Anaerobe Agar, Oxoid Preparation:
Suspend 43g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

Carry out plate counts of microorganisms in water with Thermo Scientific™ Oxoid™ Yeast Extract Agar (Dehydrated). Yeast Extract Agar is made to the formula described by Windle Taylor for the plate count of microorganisms in water and is tested in accordance with ISO 1133:2014. It is a rich, nutritious medium for the recovery of a wide range of bacteria, yeast, and molds.

Yeast Extract Agar, Oxoid Composition

Yeast Extract Agar, Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Cultivate and enumerate anaerobes, especially Clostridium species, with Thermo Scientific™ Oxoid™ Reinforced Clostridial Agar (RC Agar) (Dehydrated). A solid, highly nutritious medium, it can be used as an enrichment medium to optimize the growth of clostridia and other anaerobes, lactobacilli, and many other species of bacteria.

Reinforced Clostridial Agar (RC Agar) (Dehydrated), Oxoid Composition

Reinforced Clostridial Agar (RC Agar) (Dehydrated), Oxoid Preparation:
Suspend 52.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Further identification tests must be carried out on organisms isolated from this medium.

Isolate and enumerate coliforms in food, animal feed and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), while adhering to ISO 4832:2006 and ISO/TS 11133-2:2003. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Composition

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBL Agar (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBL Agar (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBL Agar (ISO) plates can be stored for up to 2 weeks when made from CM0968 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBL Agar (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBL Agar (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

• Oxoid Rose-Bengal Chloramphenicol Agar Base is a medium for the selective enumeration of yeast and molds from foods.
• Also available Chloramphenicol Selective Supplement, Part No. SR0078E.

Rose-Bengal Chloramphenicol Agar Base, Oxoid Composition

Rose-Bengal Chloramphenicol Agar, Oxoid Preparation:
Suspend 16.0g per 500ml (32.0g/l) of distilled water and bring to the boil to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to Rose Bengal Chloramphenicol Agar Base (CM0549) and mix gently. Autoclave at 121°C for 5 minutes. Cool to 50°C, mix gently and pour into Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.
NB: Rose-Bengal photo-oxidises to form toxic compounds. Store plates of the medium in the dark and avoid exposure to light.

Precautions
It is essential to store plates of media containing Rose-Bengal in the dark to prevent toxic photo-oxidation of the dye. See above.
Identify moulds and yeasts by morphological appearance and microscopic examination. Colonies of bacteria and yeasts can be confused.

Oxoid HTM Supplement is used for the susceptibility testing of Haemophilus influenzae.

• Add to HTM Base, Part No. CM0898B Store at -20°C to 0°C
• Each vial supplements 500mL of medium

HTM Supplement, Oxoid Composition

Haemophilus Test Medium (HTM), Oxoid Preparation:
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
HTM Supplement as supplied should be stored in the dark below 0°C. When stored as directed the supplement is stable until the expiry date stated on the label.

• Oxoid Mycoplasma Agar Base is used for the selective isolation of Mycoplasma pneumoniae.
• Also available Mycoplasma Supplement P, Part No. SR0060C and Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Agar Base, Oxoid Composition

Mycoplasma Agar, Oxoid Preparation:
Add 35.5g to 1 litre of distilled water. Boil to dissolve the agar and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions in the HAZARDS section.
Do not use thallous acetate media to isolate Ureaplasma urealyticum.
Mycoplasmas may be suspected if (1) typical morphology (2) no growth in media without serum (3) colonies are embedded below the agar surface.

A selective supplement for the detection, enumeration and presumptive identification of Listeria
monocytogenes and other Listeria species from food, animal feed and environmental samples
according to ISO 11290-1:2017 and ISO 11290-2:2017 standards and other national reference
methods using the Ottaviani & Agosti formulation.

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Composition

Brilliance Listeria Agar (ISO) Selective Supplement, Oxoid Directions:
Aseptically add 1 vial warmed to 48°C to 480ml of sterile Brilliance™ Listeria Agar base (ISO) (CM1212) prepared as directed, cooled to 48°C and add 1 vial of Brilliance™ Listeria Selective Supplement (SR0257E) reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life

Store the selective (SR0257)  supplements at 2-8°C away from light. Ensure all product is used before the expiry date.

Oxoid Membrane Lauryl Sulphate Broth is a direct replacement for Membrane Enriched Teepol Broth.

Membrane Lauryl Sulphate Broth, Oxoid Composition

Membrane Lauryl Sulphate Broth, Oxoid Preparation:
Dissolve 76.2 grams in 1 litre of distilled water. Distribute into final containers, e.g. 100 ml screw cap bottles. Sterilise by steaming for 30 minutes on three consecutive days or by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium below 25°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Mycoplasma Broth Base is used for the selective isolation of Mycoplasma species.
• Also available Mycoplasma Supplement G, Part No. SR0059C.

Mycoplasma Broth Base, Oxoid Composition

Mycoplasma Broth, Oxoid Preparation:
Dissolve 25.5g in 1 litre of distilled water. Mix well and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Thallous acetate is toxic, observe the precautions stated under Hazards section..
Sub-culture the broth to agar as soon as any indicator added begins to change colour before the pH change destroys the organism.

Oxoid Perfringens SFP Supplement is for the selective isolation of Clostridium perfringens.

• Add to Perfringens Agar Base, TSC, Part No. CM0587B
• Each vial supplements 500mL of medium

Perfringens SFP Supplement, Oxoid Composition

Shahidi-Ferguson Perfringens Agar (SFP Agar) Preparation:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid K-F Streptococcus Agar is a selective medium for the isolation and enumeration of Group D Streptococci.
• Also available TTC Solution, Part No. SR0148A.

K-F Streptococcus Agar Base, Oxoid Composition

K-F Streptococcus Agar, Oxoid Preparation:
Suspend 38.2g in 500ml of distilled water. Bring to the boil with frequent agitation. Boil for 5 minutes. Cool to 50°C and add aseptically 1 vial (5ml) of 1% 2,3,5-Triphenyltetrazolium chloride (SR0229K). Pour into sterile Petri dishes when using the membrane filtration method or hold at 45°C when using the pour-plate method.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
Observe the HAZARD precautions regarding sodium azide when disposing of the medium. The pH of the medium should not fall below 7.0 as it may become inhibitory towards enterococci. KF Streptococcus Agar is not specific for the presumptive identification of Group D streptococci. Further tests must be made to confirm the identity of the organisms isolated.

Cultivate streptococci, Neisseria and other fastidious organisms with Thermo Scientific™ Oxoid™ Brain Infusion Broth (Dehydrated). The medium is highly nutritious and well buffered to support the growth of wide variety of organisms. It is recommended for blood culture work for the isolation and cultivation of pathogenic fungi.

Brain Infusion Broth, Oxoid Composition

Brain Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store tubed or bottled medium in the dark and below 20°C.

Isolate and differentiate Shigella and Salmonella spp. from enteric pathological specimens with Thermo Scientific™ Oxoid™ Hektoen Enteric Agar (Dehydrated). Hektoen Enteric Agar was developed by King and Metzger. When compared to other selective media this medium gives higher yield of Salmonella and Shigella, while inhibiting accompanying Gram-positive and other microorganisms.

Hektoen Enteric Agar, Oxoid Composition

Hektoen Enteric Agar, Oxoid Preparation:
Suspend 76g of the medium in 1 litre of distilled water and soak for 10 minutes. Heat gently and allow to boil for a few seconds to dissolve the agar. DO NOT AUTOCLAVE. Cool to 50°C and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
Do not overheat the medium or hold it molten for long periods. Proteus species may resemble salmonellae or shigellae.
Further testing must be carried out to confirm the presumptive identification of organisms isolated on this medium.

Cultivate fastidious organisms with highly nutritious Thermo Scientific™ Oxoid™ Brain Heart Infusion Agar (Dehydrated). Brain Heart Infusion Agar is recommended for the cultivation of Streptococci, Neisseria and other fastidious organisms.

Brain Heart Infusion Agar, Oxoid Composition

Brain Heart Infusion Agar, Oxoid Preparation:
Suspend 47g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes. For blood agar, cool to 50°C and enrich with 10% v/v sterile defibrinated blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
The medium is tested for compatibility using 7% v/v oxalated horse blood, defibrinated horse blood or defibrinated sheep blood. There should be no evidence of lysis or darkening after incubation at 37°C, 25°C or 4°C for 72 hours.
When using this medium to isolate Histoplasma capsulatum, Coccidioides immitis or other pathogenic fungi which can produce free infective spores, extreme care must be taken to avoid dissemination of infective particles in the laboratory. The cultures should be examined only in a closed, filtered air cabinet.

This medium is unsuitable for the screening of vancomycin resistant enterococci (VRE). More suitable and superior media for the screening of VRE are available; Brilliance VRE PO1226A or Remel BHI with Vancomycin RO1176.

• Brilliance is a chromogenic medium for the isolation and differentiation of Bacillus cereus in food samples.
• Add to Brilliance Bacillus Cereus Agar Base, Part No. CM1036A and CM1036B. Each vial supplements 500mL of medium.

Brilliance Bacillus Cereus Selective Supplement, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Oxoid Listeria Enrichment Broth Base (UVM Formulation) is a two-step selective enrichment (USDA-FSIS) method.
• Also available Listeria Primary Enrichment Supplement-UVM I, Part No. SR0142E or SR0142B and Listeria Secondary Selective Enrichment Supplement-UVM II, Part No. SR0143E.
  Listeria Enrichment Broth Base (UVM formulation) is to be used with the appropriate supplement for UVMI broth or UVMII broth.

Listeria Enrichment Broth Base (UVM formulation), Oxoid Composition

Listeria Enrichment Broth, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.

To Prepare Listeria Primary Selective Enrichment Medium (UVM I):
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

To Prepare Listeria Secondary Selective Enrichment Medium (UVM II):
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

• Oxoid DRBC Agar Base (Dichloran Rose-Bengal Chloramphenicol Agar Base) is a selective medium for yeast and molds associated with food spoilage.
• Also available Chloramphenicol Selective Supplement, Part No. SR0078E and Chloramphenicol Selective Supplement, Part No. SR0078H

DRBC Agar Base, Oxoid Composition

DRBC Agar, Oxoid Preparation:
Suspend 15.75g in 500ml (31.5g/l) of distilled water and heat to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the DRBC Agar Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Oxoid Fraser Supplement is used for the isolation of Listeria species.

• Add to Fraser Broth Base, Part No. CM0895B
• Each vial supplements 500mL of medium

Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.