Microbiology

• Oxoid Cronobacter Screening Broth Base (CSB) is a selective enrichment broth for isolation of Cronobacter spp, from food and environmental samples.
• Requires Vancomycin Supplement, 5mg, Part No. SR0247E.

Cronobacter Screening Broth Base (CSB), Oxoid Composition

Cronobacter Screening Broth (CSB), Oxoid Preparation:
Dissolve 14.02g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label
Store the prepared medium at 2-8°C

• Oxoid Half Fraser Supplement is used for the enrichment of Listeria species.
• Add to Fraser Broth Base, Part No. CM0895B.

Half Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid Peptone P is a peptic digest which conforms to the USP specification of peptic digests of animal tissue.

• The high sulphur content makes it ideal for use in culture media

Selectively isolate and enumerate Bacillus cereus from food samples with Thermo Scientific™ Oxoid™ Bacillus cereus Selective Agar Base (Dehydrated). Bacillus cereus Selective Agar Base meets the requirements for a medium that is sufficiently selective to be able to detect small numbers of Bacillus cereus cells and spores in the presence of large numbers of other food contaminants. The medium is also sufficiently diagnostic that colonies of Bacillus cereus are readily identified and confirmed by microscopic examination.

Bacillus cereus Selective Agar Base, Oxoid Composition

Bacillus cereus Selective Agar Base, Oxoid Preparation:
Suspend 20.5g in 475ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Polymyxin B Supplement (SR0099) reconstituted as directed, then add 25ml of sterile Egg Yolk Emulsion (SR0047). Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and Polymixin B Supplement at 2-8°C. Use before the expiry date on the label.
The prepared medium may be stored at 2-8°C.

Precautions
On this medium Bacillus cereus is indistinguishable from Bacillus thuringiensis.
Identify Bacillus cereus by colony form, colour, egg yolk hydrolysis and confirm with cell and spore morphology.
Occasional strains of Bacillus cereus show weak or negative egg yolk reactions.

• Oxoid Dichloran-Glycerol (DG18) Agar Base is a selective low water activity (aw) medium for xerophilic molds from dried and semi-dried foods.
• Also available Chloramphenicol Selective Supplement, Part No. SR0078E and Chloramphenicol Selective Supplement, Part No. SR0078H.

Dichloran-Glycerol (DG18) Agar Base, Oxoid Composition

Dichloran-Glycerol (DG18) Agar, Oxoid Preparation:
Suspend 15.75g in 500ml (31.5g/l) of distilled water and heat to dissolve completely. Add 110g per 500ml (220g/l) of Glycerol (Analytical Reagent grade). Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the DG18 Agar Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

• Oxoid Yersinia Selective Agar Base (Schiemann’s CIN Agar) is used for the isolation and enumeration of Yersinia enterocolitica from clinical specimens and foods.
• Also available Yersinia Selective Supplement, Part No. SR0109E. Industrial Reference: FDA.

Yersinia Selective Agar Base, Oxoid Composition

Yersinia Selective Agar, Oxoid Preparation:
Suspend 29g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to approximately 50°C and aseptically add the contents of one vial of Yersinia Selective Supplement SR0109 reconstituted as directed in the instructions for use that accompany the product. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 24 hours.

Precautions
Some strains of Yersinia enterocolitica may grow poorly or not at all. Other species of Yersinia may grow along with some enteric organisms. It is therefore essential that full identification tests are carried out on suspect colonies.

Oxoid Novobiocin Selective Supplement (10mg/vial) is added to EC Broth, Part No. CM0990B; Muller-Kauffmann Tetrathionate-Novobiocin Broth Base, Part No. CM0343B; Brilliant Green Agar, Part No. CM0263B; MRSV, Part No. CM0910B; Modified Tryptone Soya Broth, Part No. CM0989B; and Lysine Iron Agar, Modified, Part No. CM0886B.

• Each vial supplements 500mL of medium

Novobiocin Supplement, Oxoid Composition

Novobiocin Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to the designated medium cooled to approximately 50°C. Mix well and dispense into sterile containers.

Storage conditions and Shelf life
Novobiocin Supplement SR0181 should be stored in the dark at 2-8°C. When stored as directed, the supplement remains stable until the expiry date printed on the label.

Application

For maintenance of Lactobacilli and for determining the action of bacteria on milk.

Final pH: 6.8±0.2 (25 °C)

• Oxoid Campylobacter Karmaili Agar Base is a blood-free selective medium for the isolation of Campylobacter jejuni and Campylobacter coli when incubated at 42°C.
• Also available Campylobacter Selective Supplement (Karmaili), Part No. SR0167E and Modified Karma-i-li Selective Supplement, Part No. SR0205E.

Campylobacter Karmaili Agar Base, Oxoid Composition

Campylobacter Agar (Karmali), Oxoid Preparation:
Add 21.5g of Campylobacter Agar Base (Karmali) to 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of Campylobacter Selective Supplement (Karmali) SR0167 or Modified Karmali Selective Supplement SR0205, reconstituted as directed.. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Campylobacter Agar Base (Karmali) should be stored tightly capped in the original container in a cool, dry place away from bright light. When stored at 10-30°C the medium will remain stable until the expiry date printed on the label.

Cultivate and enumerate clostridia and other anaerobes occurring in food and pathological samples with Thermo Scientific™ Oxoid™ Reinforced Clostridial Medium. A semi-solid, highly nutritious medium can be used as an enrichment medium to optimize the growth of clostridia before sub-culturing to solid agar.

Reinforced Clostridial Medium, Oxoid Composition

Reinforced Clostridial Medium, Oxoid Preparation:
Suspend 38 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Mueller Hinton Broth, Oxoid conforms to international standards for antimicrobial susceptibility testing media.

Mueller Hinton Broth, Oxoid Preparation
Place 21.0g of Mueller Hinton Broth, Oxoid in 1 litre of distilled water mix to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Chill and adjust cation levels if necessary.

Storage conditions and Shelf life
Store the dehydrated Mueller Hinton broth at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton broth at 2-8°C.

Precautions
Monitor the performance of the broth routinely using the standard QC organisms. If the broth does not yield the expected MIC values, modify the volumes of Mg++ and Ca++ solutions.

If the thymidine content is lowered, after the addition of lysed horse blood or thymidine phosphorylase, the MIC values may be lower.

Detect coliform organisms in water and milk examination with Thermo Scientific™ Oxoid™ MacConkey Broth Purple (Dehydrated). Bromocresol purple indicator in the medium provides more sensitive and definite indications of acid production as compared to neutral red indicator and is less inhibitory to Escherichia coli. The use of bromocresol purple corresponds to the alternative formulations recommended in ‘The Bacteriological Examination of Water Supplies’ and ‘International Standard for Drinking Water’.

MacConkey Broth Purple, Oxoid Composition

MacConkey Broth Purple, Oxoid Preparation:
To prepare single strength broth add 40g to 1 litre of distilled water. Distribute into containers fitted with fermentation (Durham) tubes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Bolton Broth Base, Oxoid Composition

Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.

Thermo Scientific™ Oxoid Tryptose is a mixed enzymatic hydrolysate used for the growth of many fastidious microorganisms.

Thermo Scientific™ Oxoid XLT-4 Selective Supplement is a selective supplement for use with XLT-4 Agar.

XLT-4 Selective Supplement, Oxoid Composition

XLT-4 Agar, Oxoid Preparation:
Suspend 59g of XLT-4 Agar Base in 1 litre of distilled water, add 4.6ml of XLT-4 Selective Supplement and bring the medium to the boil.
Do not overheat, do not autoclave.
Cool to approximately 50°C and pour into sterile Petri dishes. It is not advised to hold the medium at 50°C for longer than 1 hour as this may cause the medium to precipitate.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Isolate Listeria spp. from food and environmental specimens using Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated). The medium is intended for the isolation of Listeria spp. when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Fraser Broth enrichment cultures should be sub-cultured to plating medium.

Fraser Broth Base, Oxoid Composition

Fraser Broth, Oxoid Preparation:
With Fraser Supplement:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

With Half Fraser Supplement:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers. Alternatively, to make 2.25 litres, use 129.0g of CM0895 and 1 vial of SR0166G.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid Lactalbumin Hydrolysate is a pancreatic digest of lactalbumin which contains high levels of essential amino acids.

Select and identify salmonellae (other than Salmonella Typhi) from food, feed and water samples with Thermo Scientific™ Oxoid™ Modified Brilliant Green Agar (Dehydrated). The modified formula provides improved inhibition of Escherichia coli and Proteus species. The medium has been widely assessed in Europe and has been used in ISO standards.

Brilliant Green Agar (Modified), Oxoid Composition

Brilliant Green Agar (Modified), Oxoid Preparation:
Suspend 52g in 1 litre of distilled water. Heat gently with occasional agitation and bring just to the boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C, mix well and pour plates.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Lactose-fermenting salmonellae may be present in foods.
Salmonella typhi and Shigella species may not grow on this medium.
Proteus, Citrobacter and Pseudomonas species may mimic enteric pathogens by producing small red colonies.

Cultivate and isolate fastidious microorganisms from clinical samples through improved hemolytic reactions with sheep blood with Thermo Fisher™ Oxoid™ Sheep Blood Agar Base (Dehydrated) when used with sheep blood. Sheep Blood Agar Base was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of organisms without interfering with their hemolytic reactions when used with sheep blood.

Sheep Blood Agar Base (Dehydrated), Oxoid Composition

Sheep Blood Agar Base (Dehydrated), Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 7% sterile sheep blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.