Culture Media Products

Oxoid CCDA Selective Supplement is used for the isolation of Campylobacter spp., especially C. upsaliensis or Arcobacter.

• For the isolation of Arcobacter spp., add to Arcobacter Broth Base, Part No. CM0965B
• For the isolation of Campylobacter spp., especially C. upsaliensis, add to Campylobacter Blood-Free Selective Agar Base (CCDA Preston), Part No. CM0739B

CCDA Selective Supplement, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Oxoid CCDA Selective Supplement is used for the isolation of Campylobacter spp., especially C. upsaliensis or Arcobacter.

• For the isolation of Arcobacter spp., add to Arcobacter Broth Base, Part No. CM0965B
• For the isolation of Campylobacter spp., especially C. upsaliensis, add to Campylobacter Blood-Free Selective Agar Base (CCDA Preston), Part No. CM0739B

CCDA Selective Supplement, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Oxoid CDMN Supplement is used for the growth of Clostridium difficile.

• Add to to C. Difficile Agar Base, Part No. CM0601B
• Each vial for 2L medium

CDMN Selective Supplement, Oxoid Composition

CDMN Agar, Oxoid Preparation:
Aseptically add 2ml of sterile distilled water to one vial of supplement and mix gently to dissolve completely. Aseptically add the contents to 500ml of sterile Clostridium difficile Agar Base CM0601 cooled to approximately 50°C together with 7% (v/v) of Defibrinated Horse Blood SR0050. Mix well and pour the resulting CDMN Agar into sterile Petri dishes.

Oxoid Cefixime Supplement is used for the isolation of Escherichia coli O157.

• Add to to Cefixime Rhamnose Sorbitol MacConkey Agar Base, Part No. CM1005B
• Each vial supplements 500mL of medium

Cefixime Supplement, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Oxoid C-T Supplement is a chromogenic medium for the identification of Escherichia coli O157:H7.
• Add to Sorbitol MacConkey Agar, Part No. CM0813B or to Sorbitol MacConkey Agar with BCIG, Part No. CM0981B.

Cefixime Tellurite Selective Supplement, Oxoid Composition

Sorbitol MacConkey Agar, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Sorbitol MacConkey Agar Base CM0813 cooled to approximately 50°C. Mix well and pour the medium into Petri dishes.

Storage conditions and Shelf life
Cefixime-Tellurite Supplement (SR0172) should be stored in the dark at temperatures below 0°C.

Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.

Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

• Oxoid Charcoal Agar Base (Bordetella Selective Medium) is used for selective isolation of B. pertussis and B. parapertussis.
• Requires addition of Bordetella Selective Supplement, Part No. SR0082E.

Charcoal Agar Base, Oxoid Composition

Charcoal Agar, Oxoid Preparation:
Suspend 51g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, add 10% of defibrinated blood and mix gently. The medium is made selective for the isolation of Bordetella pertussis and Bordetella parapertussis by the addition of Bordetella Selective Supplement SR0082.

To one vial add 2ml of sterile distilled water and dissolve the contents completely. Add this solution to 500ml of sterile, molten Charcoal Agar, cooled to 50°C, together with 10% v/v defibrinated horse blood SR0050. Mix well before pouring into sterile Petri dishes.
For Haemophilus influenzae, omit the selective agents and convert to `chocolate’ agar.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Stuart’s transport medium or similar formulation media should not be used for Bordetella-containing specimens.
Two pernasal swabs should be taken from each patient, one through each nostril.
Make sure the charcoal remains in suspension when dispensing the medium by gently swirling the flask.
Lysed horse blood is used in the transport medium but whole blood is used in the isolation medium.
Most naso-pharyngeal flora are inhibited by cephalexin but Pseudomonas aeruginosa and some fungi may grow through. Amphotericin B can be added (12mg/ml) as an antifungal agent to prevent the growth of filamentous fungi. However, this level of amphotericin B can be inhibitory to Bordetella pertussis and should not be used routinely.

Enumerate and differentiate bacteria in dairy products with this standard Thermo Scientific™ Oxoid™ China Blue Lactose Agar (Dehydrated). China Blue Lactose Agar, originally formulated by Brandl and Sobeck-skal, is a standard non-inhibitory solid medium used for the differentiation of lactose fermenters from non-lactose fermenters in milk.

The china blue in the medium serves as a pH indicator to differentiate lactose fermenters from non-lactose fermenters. The medium does not contain any inhibitory substances therefore all the organisms present in milk sample grow luxuriantly on this medium.

China Blue Lactose Agar Base, Oxoid Composition

China Blue Lactose Agar, Oxoid Preparation:
Suspend 35g in 1 litre of distilled water. Boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is important to remember that Gram positive and negative cocci and bacilli can grow on this medium. Always confirm the organism morphology and Gram reaction.

For the isolation of yeast and molds associated with spoilage, add Oxoid Chloramphenicol Selective Supplement to DRBC Agar Base, Part No. CM0727B; for the enumeration of yeast and molds in foods, add to Rose Bengal Chloramphenicol Agar Base, Part No. CM0549B.

Chloramphenicol Selective Supplement, Oxoid Composition

Chloramphenicol Selective Medium, Oxoid Preparation:
Reconstitute one vial SR0078H per 2 litres medium as directed. Add the vial contents to base medium and mix gently. Sterilise by autoclaving (see media for full details), cool to 50°C, mix gently and pour into Petri dishes.

For the isolation of yeast and molds associated with spoilage, add Oxoid Chloramphenicol Selective Supplement to DRBC Agar Base, Part No. CM0727B; for the enumeration of yeast and molds in foods, add to Rose Bengal Chloramphenicol Agar Base, Part No. CM0549B.

Chloramphenicol Selective Supplement, Oxoid Composition

Chloramphenicol Selective Medium, Oxoid Preparation:
Reconstitute one vial SR0078E per 500ml medium as directed. Add the vial contents to base medium and mix gently. Sterilise by autoclaving (see media for full details), cool to 50°C, mix gently and pour into Petri dishes.

This product has been discontinued, please refer to CM1212B

Isolate, enumerate and differentiate Listeria species and Listeria monocytogenes from food samples with Thermo Scientific™ Oxoid™ Chromogenic Listeria Agar (ISO) (Dehydrated). Chromogenic Listeria Agar (ISO) contains lithium chloride, polymyxin B, nalidixic acid, and amphotericin which act to inhibit non-target organisms. A combination of chromogenic and phospholipase enzyme reactions easily differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).

Chromogenic Listeria Agar (ISO), Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

• Oxoid Chromogenic Salmonella Agar Base is a selective chromogenic medium for the presumptive identification of Salmonella spp. from clinical and food samples.
• Also available Salmonella Selective Supplement, Part No. SR0194E and Brilliance™ Salmonella Agar Base, Part No. CM1092B.

Chromogenic Salmonella Agar Base, Oxoid Composition

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 6 weeks at 2-8°C.

• Oxoid Clostridium Difficile Selective Supplement is added to Clostridium Difficile Agar Base, Part No. CM0601B.
• Each vial supplements 500mL of medium.

Clostridium Difficile Selective Supplement, Oxoid Composition

Clostridium Difficile Agar, Oxoid Preparation:
Suspend 34.5g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and add aseptically the contents of 1 vial of Oxoid Clostridium Difficile Supplement (SR0096) reconstituted as directed, together with 7% (v/v) Defibrinated Horse Blood (SR0050). Sheep Blood (SR0051) may be used in place of Horse Blood but some strains of the organism will show a slightly reduced growth recovery. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C no longer than 5-7 days.

• Oxoid Clostridium Difficile Agar Base is used for the isolation of Clostridium difficile.
• Also available Clostridium Difficile Selective Supplement, Part No. SR0096E.

lostridium Difficile Agar Base, Oxoid Composition

Clostridium Difficile Agar, Oxoid Preparation:
Suspend 34.5g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and add aseptically the contents of 1 vial of Oxoid Clostridium Difficile Supplement (SR0096) reconstituted as directed, together with 7% (v/v) Defibrinated Horse Blood (SR0050). Sheep Blood (SR0051) may be used in place of Horse Blood but some strains of the organism will show a slightly reduced growth recovery. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C no longer than 5-7 days.

Precautions
Colonies of Clostridium difficile from faecal cultures are smaller when egg yolk is used in place of horse blood.

The Oxoid formula does not contain the neutral red indicator proposed by George et al. because it is designed for use with horse blood. On this medium the typical colour of the colony of Clostridium difficile will not appear however there will be a fluorescent reaction.

Typical Gram stain morphology of Clostridium difficile may not be evident in colonies picked from this medium because of the antibiotics present. Subculture to blood agar to obtain characteristic morphology.

Cultivate and isolate fastidious microorganisms with clearly visible hemolytic reactions with Thermo Scientific™ Oxoid™ Columbia Blood Agar Base (Dehydrated) when used with whole defibrinated blood (SR0050). Columbia Blood Agar Base is an ideal base medium for preparation of blood agar, chocolate agar and for preparation of various selective and identification media.

Columbia Blood Agar Base, Oxoid Composition

Columbia Blood Agar, Oxoid Preparation:
Add 39g to 1 litre of distilled water. Boil to dissolve the medium completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5% sterile defibrinated blood.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.

Precautions
Brucella cultures are highly infective and must be handled under properly protected conditions. Incubate in 5-10% carbon dioxide atmosphere for 24-48 hours.
Campylobacter species are best grown at 42°C (except Campylobacter fetus subsp. fetus) in a micro-aerophilic atmosphere (Oxoid Campylobacter Gas Generating Kit BR0056 or BR0060 or CampyGen CN0025/CN0035).
Staph./Strep. supplemented plates should be incubated aerobically at 35°C for 18 hours. Incubation in carbon dioxide- enriched air will cause inhibition of staphylococcal growth.
Streptococcus Selective Supplement SR0126 supplemented plates may be incubated aerobically or anaerobically at 35°C for 18 hours.
Prepared plates of both supplemented media should be used within 18 hours of preparation for maximum selectivity. Gardnerella supplemented plates should be incubated at 35°C for 48 hours in an atmosphere containing 7% carbon dioxide.
Carry out confirmatory tests on all colonies from horse blood medium and on beta-haemolytic colonies from human or rabbit blood medium.
Incubate plates of Clostridium E-Y Agar anaerobically at 35°C for 18 hours, look for lecithinase activity (pearly layer) and for proteolysis. Lecithinase activity is inhibited in the presence of specific anti-toxin.

Grow and maintain aerobic and anaerobic organisms with Thermo Scientific™ Oxoid™ Cooked Meat Medium (Dehydrated). The medium contains heart tissue, beef extracts, peptone and glucose for the cultivation of anaerobic and aerobic organisms. It has the ability to initiate bacterial growth from very small inocula and to maintain the viability of cultures over long periods of time1. Mixed cultures of bacteria survive in Cooked Meat Medium without displacing the slower growing organisms.

Cooked Meat Medium, Oxoid Composition

Cooked Meat Medium, Oxoid Preparation:
Suspend 10g in 100ml of distilled water (or 1g amounts in 10ml volumes of water in tubes). Allow to stand for 15 minutes until the meat particles are thoroughly wetted. Sterilise by autoclaving at 121°C for 15 minutes. Do not cool the bottles rapidly because ebullition will expel the meat particles from the containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature, in the dark with tightened caps for up to 6 months.

Precautions
The excellent recovery properties of Cooked Meat Medium mean that mixed cultures commonly result from sample inoculation.
Blackening of the medium will not take place if the pH is acid.
Carbohydrate fermentation may inhibit proteolysis.

Oxoid Corn Meal Agar Mycological medium is used for identification of Candida albicans and maintenance of fungal stock cultures.

Corn Meal Agar, Oxoid Composition

Corn Meal Agar, Oxoid Preparation:
Suspend 17g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Glucose supplemented Corn Meal Agar should not be used for chlamydospore production.
Corn Meal Agar with `Tween 80’ (or other wetting agents) will allow Candida stellatoides and Candida tropicalis to produce chlamydospores.
Some Candida strains lose their ability to produce chlamydospores after repeated subculturing.

• Oxoid Cefixime Rhamnose Sorbitol MacConkey Agar Base (CR-SMAC) is a selective and differential medium containing rhamnose and cefixime for the isolation of E. coli O157.
• Also available Cefixime Supplement, Part No. SR0191E.

CR-SMAC Agar Base, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

• Oxoid Cronobacter Screening Broth Base (CSB) is a selective enrichment broth for isolation of Cronobacter spp, from food and environmental samples.
• Requires Vancomycin Supplement, 5mg, Part No. SR0247E.

Cronobacter Screening Broth Base (CSB), Oxoid Composition

Cronobacter Screening Broth (CSB), Oxoid Preparation:
Dissolve 14.02g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label
Store the prepared medium at 2-8°C

A simple medium originally described by Crossley for the routine examination of canned food samples for anaerobic bacteria, this medium has evolved as the result of comparative trials carried out by Crossley with several standard media. It is capable of giving rapid growth without the use of special anaerobic apparatus, yet the bacteria detected may be provisionally identified by their reactions upon the medium.

Crossley milk medium, Oxoid Composition

Crossley milk medium, Oxoid Preparation:
Cream 110g of the powder with a little distilled water and gradually dilute to 1 litre with continuous mixing. Tube in 10ml quantities and autoclave at 121°C for 5 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

• Oxoid Cycloheximide Solution (0.1%) (100mg/vial) is used for the suppression of fungi.
• Add to Bacillus Cereus Selective Agar Base, Part No. CM0617B; Raka-Ray Agar Base, Part No. CM0777B; and Universal Beer Agar, Part No. CM0651B.

Oxoid Czapek Dox Agar Modified is used for cultivation of fungi.

Czapek Dox Agar Modified, Oxoid Composition

Czapek Dox Agar Modified, Oxoid Preparation:
Suspend 45.4g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. If required, adjust the reaction to pH 3.5 ± 0.2 by adding 10ml of Lactic Acid 10% (SR0021) per litre after sterilisation.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Oxoid Czapek Dox Liquid Medium is used for cultivation of fungi.

Czapek Dox Liquid Medium, Oxoid Composition

Czapek Dox Liquid Medium, Oxoid Preparation:
Add 33.4g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.
Store tubes of broth at 15-25°C.

DCLS Agar is a modified form of Desoxycholate Citrate Agar which includes sucrose in its formulation. The addition of this fermentable carbohydrate increases the usefulness of the medium because non-pathogenic sucrose-fermenting organisms may be recognised by their red colonies, e.g. some Proteus, Enterobacter and Klebsiella species.

DCLS Agar, Oxoid Composition

DCLS Agar, Oxoid Preparation:
Suspend 50g in 1 litre of distilled water. Bring to the boil to dissolve the medium. Cool to 50°C and pour into sterile Petri dishes.
DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
Boil the medium for the minimal period of time to get the agar into solution. Overheating reduces the agar gel strength and increases the degree of inhibition. It is therefore important not to hold the molten medium at 50°C for more than the short time required to distribute it into dishes.

Thermo Scientific™ Oxoid Defibrinated Horse Blood is a blood product.

**This product is not available at the moment.