Microbiology

Thermo Scientific™ Oxoid Potassium Lactate is a solution for addition to culture media, Lysine Medium CM0191 for example.

Thermo Scientific™ Oxoid Lactic Acid is a solution for addition to culture media, primarily to lower the pH.

Thermo Scientific™ Oxoid MOPS-BLEB Broth Base is a medium for using with BAX Listeria species.

Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (ISO) (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium and is tested in accordance with ISO 6579 and ISO 11133:2014. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.

Buffered Peptone Water (ISO), Oxoid Composition

Buffered Peptone Water (ISO), Oxoid Preparation:
Add 20.0g of Buffered Peptone Water (ISO) to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Method of use
The sample is added to Buffered Peptone Water (ISO) at a ratio of 1:10, and incubated at 36°C ± 2°C for 16-20 hours before transfer to selective enrichment media.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Thermo Scientific™ Oxoid Brilliance Bacillus Cereus Agar is a chromogenic medium for the isolation and differentiation of Bacillus cereus from food samples.

Brilliance Bacillus Cereus Agar Base, Oxoid Composition

Brilliance Bacillus Cereus Agar, Oxoid Preparation:
Suspend 20.5g in 500ml of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and aseptically add 1 vial of Brilliance Bacillus cereus Selective Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Brilliance Bacillus cereus Selective Supplement must be stored at 2-8°C.
Store the prepared medium for up to 2 weeks at 2-8°C.

Precautions
X-glucopyranoside negative B. cereus strains may present white colonies on this medium due to poor or no utilisation of the chromogen.

Thermo Scientific™ Oxoid Brilliance™ UTI Agar (formerly Chomogenic UTI Agar ) is a chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections (UTIs).

Brilliance UTI Agar, Oxoid Composition

Brilliance UTI Agar, Oxoid Preparation:
Suspend 56.3g of Brilliance UTI Agar in 1 litre of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before pouring plates.

Storage conditions and Shelf life
Dehydrated Brilliance UTI Agar must be stored tightly capped in the original container at 10-30ºC.
Oxoid Brilliance UTI Agar plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light.

When stored as directed, the unopened product will remain stable until the expiry date on the label. Locally prepared media can be stored for up to 2 weeks when made from CM0949 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

This product has been discontinued. The alternative to this product is Sensitest Agar, 500g.

A medium specially designed to give large, clear zones with all antibiotics, without the addition of lysed or whole blood.

Sensitest Agar, Oxoid Composition

Sensitest Agar, Oxoid Preparation:
Suspend 32g in 1 litre of distilled water and bring to the boil to dissolve the agar. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of agar at 2-8°C.

Precautions
As with other susceptibility testing media, Sensitest Agar should be used for rapidly growing aerobic organisms only. It should not be modified by the addition of carbohydrates or incubated in a CO2 enriched atmosphere.
If the medium is used for Bell’s CDS method then the specified discs and technique must be used. Refer to the monograph cited in the References.

Thermo Scientific™ Oxoid Thioglycollate Medium is tested according to EP/USP.

Thioglycollate Medium, Oxoid Composition

Thioglycollate Medium, Oxoid Preparation:
Suspend Thioglycollate medium (29.75 grams/ 1itre) in de-ionised water. Heat to dissolve. Cool and dispense 100ml into final containers, 100ml DIN bottles. Sterilise at 121°C for 15 minutes. When cooled label each bottle and pack in units of 10 into labelled boxes.

Storage conditions and Shelf life
Store away from light between 2-25ºC. Product shelf life from date of production is 1 year.

Thermo Scientific™ Oxoid Tryptone Soya Broth is tested according to EP/USP.

Tryptone Soya Broth, Oxoid Composition

*Contains papain

Tryptone Soya Broth, Oxoid Preparation:
Add 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium away form the light at room temperature.

The Oxoid ONE Broth-Listeria is for the selective enrichment broth for Listeria species from food samples in 24 hours.

One Broth-Listeria Selective Supplement
ONE Broth-Listeria is the selective enrichment broth used in the Oxoid Listeria Precis™ rapid culture method. For information simply download the ONE Broth-Listeria data sheet (273KB) or the Listeria Precis data sheet (680KB) in PDF format.

ONE Broth-Listeria, Oxoid Preparation:
Suspend 22g of ONE Broth-Listeria Base in 500ml (44g/l) of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (SR0234) reconstituted as directed.

Storage conditions and Shelf life
ONE Broth-Listeria Selective Supplement should be stored in the dark at 2-8°C.
Store the prepared medium for up to 4 weeks at 2-8°C.

This product has been discontinued, please refer to SR0258E

Oxoid Brilliance Listeria Differential Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria

• Add to Brilliance Listeria Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Oxoid Brilliance Listeria Differential Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Brilliance Listeria Selective Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria spp. in food samples.

• Add to Brilliance Listeria Selective Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Brilliance Listeria Selective Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued. Please contact us for more information.

Oxoid ORSAB Selective Supplement (Oxacillin Resistance Screening Agar Base Selective Supplement) is added to Oxacillin Resistance Screening Agar Base, Part No. CM1008B.

• Add to Oxacillin Resistance Screening Agar Base, Part No. CM1008B
• Each vial supplements 500mL of medium

ORSAB Selective Supplement, Oxoid Composition

ORSAB Selective Medium Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
ORSAB Selective Supplement should be stored at 2-8°C.
Store the prepared medium at 2-8°C.

Oxoid Cefixime Supplement is used for the isolation of Escherichia coli O157.

• Add to to Cefixime Rhamnose Sorbitol MacConkey Agar Base, Part No. CM1005B
• Each vial supplements 500mL of medium

Cefixime Supplement, Oxoid Composition

CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.

Oxoid Membrane Clostridium Perfringens Selective Supplement (m-CP) is a chromogenic medium for rapid and presumptive identification of Clostridium perfringens from water samples.

• Add to Membrane Clostridium Perfringens (m-CP) Agar Base, Part No. CM0992B
• Each vial of SR0188E supplements 500mL of medium

1M-Cp Selective Supplement, Oxoid Composition

1M-Cp Selective Supplement, Oxoid Preparation:

Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:

Storage conditions and Shelf life
m-CP Selective Supplement SR0188 should be stored at 2-8°C.
Store the prepared medium at 2-8°C.

Oxoid Meropenem Selective Supplement (1mg/vial) is used for the isolation of Vancomycin-Resistant Enterococci (VRE) and HighLevel Aminoglycoside-Resistant Enterococci (HLARE) from clinical specimens.

• Add to VRE Agar Base, Part No. CM0984B
• Each vial supplements 500mL of medium

Meropenem Selective Supplement, Oxoid Composition

Storage conditions and Shelf life
Meropenem Supplement SR0184 should be stored in the dark at 2-8°C.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Oxoid Legionella BCYE Supplement w/o L-cysteine is used for the isolation of Legionella spp.

• Add to Legionella CYE Agar Base, Part No. CM0655B
• Each vial supplements 100mL of medium

Legionella BCYE Growth Supplement without L-cysteine, Oxoid Composition

Legionella BCYE Growth Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 90ml of sterile Legionella CYE Agar Base (CM0655) cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.