Microbiology

Oxoid Listeria Selective Enrichment Supplement, Modified is used for the enrichment of Listeria spp.

• Modified with 10mg/L acriflavin
• Add to Listeria Enrichment Broth, Part No. CM0862B
• Each vial supplements 2.25L of medium

Listeria Selective Enrichment Supplement (Modified), Oxoid Composition

Listeria Selective Enrichment Medium Preparation:
Aseptically add 10ml of distilled water to one vial and invert gently to dissolve. Add the vial contents to 2.25 litres of Listeria Enrichment Broth Base. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and distribute into sterile containers in volumes as required .

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product has been discontinued, please refer to SR0258E

Thermo Scientific™ Oxoid OCLA (ISO) Differential Supplement is a differential supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Differential Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Thermo Scientific™ Oxoid OCLA (ISO) Selective Supplement is a selective supplement for use with Chromogenic Listeria Agar (ISO).

OCLA (ISO) Selective Supplement, Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid Listeria Primary Enrichment Supplement (UVMI) is a primary selective enrichment supplement for the isolation of listeria when used with Listeria Enrichment Broth Base (UVM Formulation) CM0863.

Oxoid Listeria Primary Enrichment Supplement (UVMI), Oxoid Composition

Listeria Primary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

A Bacillus cereus 569/H9 lactamase (E.C.5.2.6) presented as a freeze-dried powder containing buffer salts. Each vial contains a 3,300lU of activity (1 unit of enzyme activity will hydrolyse 1.0μmol of benzylpenicilloic acid per minute pH7.0 and at 25°C).

Used for the inactivation of susceptible beta-lacram antibiotics in preparations, blood or tissue samples.

Thermo Scientific™ Oxoid Legionella BMPA-α Selective Supplement is a selective supplement for the isolation of legionellae when used with Legionella BCYE Growth Supplement (SR0010) and Legionella CYE Agar Base (CM0655).

Legionella BMPA-α Selective Supplement, Oxoid Composition

Legionella BMPA-α Selective Medium Preparation:
Reconstitute one vial SR0111E per 100ml medium as directed. Aseptically add the vial contents to BCYE-a Medium prepared from Legionella CYE Agar Base (CM0655) and Legionella BCYE Supplement (SR0110) and cooled to 50°C. Mix well and pour into sterile Petri dishes.

Thermo Scientific™ Oxoid Defibrinated Sheep Blood is a blood product.

**This product is not available at the moment.

Thermo Scientific™ Oxoid Defibrinated Horse Blood is a blood product.

**This product is not available at the moment.

Thermo Scientific™ Oxoid Defibrinated Horse Blood is a blood product.

**This product is not available at the moment.