Microbiology

Oxoid CDMN Supplement is used for the growth of Clostridium difficile.

• Add to to C. Difficile Agar Base, Part No. CM0601B
• Each vial for 2L medium

CDMN Selective Supplement, Oxoid Composition

CDMN Agar, Oxoid Preparation:
Aseptically add 2ml of sterile distilled water to one vial of supplement and mix gently to dissolve completely. Aseptically add the contents to 500ml of sterile Clostridium difficile Agar Base CM0601 cooled to approximately 50°C together with 7% (v/v) of Defibrinated Horse Blood SR0050. Mix well and pour the resulting CDMN Agar into sterile Petri dishes.

• Oxoid Half Fraser Supplement is used for the enrichment of Listeria species.
• Add to Fraser Broth Base, Part No. CM0895B.

Half Fraser Supplement, Oxoid Composition

Fraser Broth, Oxoid Preparation:
Suspend 129.0g of Fraser Broth in 2.25 litres of distilled water, and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166G reconstituted as directed in the product insert. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Oxoid Neomycin Selective Supplement (150mg/vial) allows the growth of clostridia and other anaerobes.

• Add to Blood Agar, Wilkins-Chalgren Anaerobe Agar, Part No. CM0619B, or Columbia Blood Agar, Part No. CM0331B
• Each vial supplements 2L of medium

Neomycin Selective Supplement, Oxoid Composition

Neomycin Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 2 litres of Wilkins Chalgren Agar Base CM0619 or another appropriate medium cooled to approximately 50° C. Mix well and pour into sterile Petri dishes.

Oxoid CCDA Selective Supplement is used for the isolation of Campylobacter spp., especially C. upsaliensis or Arcobacter.

• For the isolation of Arcobacter spp., add to Arcobacter Broth Base, Part No. CM0965B
• For the isolation of Campylobacter spp., especially C. upsaliensis, add to Campylobacter Blood-Free Selective Agar Base (CCDA Preston), Part No. CM0739B

CCDA Selective Supplement, Oxoid Composition

Campylobacter Blood-Free Selective Agar, Oxoid Preparation:
Suspend 22.75g of Campylobacter Blood-Free Selective Agar Base in 500ml of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 1 vial of CCDA Selective Supplement SR0155 reconstituted as directed. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C .

Oxoid Legionella GVPC Selective Supplement is used for the isolation of Legionella spp. from environmental samples.

• Add to Legionella CYE Agar Base, Part No. CM0655B
• Each vial supplements 500mL of medium

Legionella GVPC Selective Supplement, Oxoid Composition

Legionella GVPC Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Legionella CYE Agar Base CM0655 to which one vial of Legionella BCYEa Legionella Growth Supplement SR0110C has been added cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid STAA Selective Supplement is used for the isolation of Brochothrix thermosphacta from food samples.

• Add to STAA Agar Base, Part No. CM0881B
• Each vial supplements 500mL of medium

STAA Selective Supplement, Oxoid Composition

STAA Selective Medium Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.

Storage conditions and Shelf life
Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.

Precautions
STAA Selective Supplement SR0151 contains cycloheximide and thallous acatate and is toxic if swallowed, inhaled or if in contact with skin.

Oxoid Helicobacter Pylori Selective Supplement (Dent) is for the isolaton of Helicobacter pylori from clinical specimens.

• Used with Columbia Blood Agar Base, Part No. CM0331B
• Each vial supplements 500mL of medium

Helicobacter Pylori Selective Supplement (Dent), Oxoid Composition

Helicobacter Pylori Selective Medium, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of Columbia Blood Agar Base cooled to approximately 50°C. Add 35 ml of Laked Horse Blood SR0048 and mix well before pouring into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Listeria Secondary Selective Enrichment Supplement, UVMII is used in a two-step selective enrichment (USDS-FSIS) method for the enrichment of Listeria spp.

• Add to Listeria Enrichment Broth Base (UVM formulation), Part No. CM0863B
• Each vial supplements 500mL of medium

Listeria Secondary Selective Enrichment Supplement (UVMII), Oxoid Composition

Listeria Secondary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

This product has been discontinued. The recommended alternative is Modified Preston Campylobacter Supplement, SR0204E.

Oxoid Preston Campylobacter Selective Supplement is used for the isolation of Campylobacter jejuni and Campylobacter coli from human, animal, avian, and environmental samples.

• Add to Campylobacter Agar Base, Part No. CM0689B or Nutrient Broth No. 2, Part No. CM0067B
• Each vial supplements 500mL of medium

Preston Campylobacter Selective Supplement, Oxoid Composition

Preston Campylobacter Selective Agar, Oxoid Preparation:
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.

Preston Campylobacter Selective Enrichment Broth, Oxoid Preparation:
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Thermo Scientific™ Oxoid Nitrocefin is used for detection of beta-lactamase production.

• Reconstitutes to 500μg/mL
• Contents: 5 vials lyophilized nitrocefin (1mg) and 5 vials rehydrating fluid (1.9mL)

• Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
• Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

• Oxoid Legionella BCYE Supplement is used for the isolation of Legionella spp.
• Add to Legionella CYE Agar Base, Part No. CM0655B.

Legionella BCYE Growth Supplement, Oxoid Composition

BCYE Agar, Oxoid Preparation:
Suspend 2.5g of Legionella CYE Agar Base per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Storage conditions and Shelf life
Store the dehydrated medium below 30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

This product is not available at the moment. Please contact us for more information.

Oxoid G-N Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

G-N Anaerobe Selective Supplement, Oxoid Composition

Selective Medium for Gram-Negative Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of G-N Anaerobe Supplement (SR0108) rehydrated as directed, together with 25ml defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

This product is not available at the moment. Please contact us for more information.

Oxoid N-S Anaerobe Selective Supplement is used for the growth of fastidious and other anaerobes.

N-S Anaerobe Selective Supplement , Oxoid Composition

Selective Medium for Non-Sporing Anaerobes Preparation:
Suspend 21.5g of Wilkins-Chalgren Anaerobe Agar (CM0619) in 475ml of distilled water containing 0.5ml `Tween 80’. Bring to the boil to dissolve completely and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add the contents of 1 vial of N-S Anaerobe Supplement (SR0107) rehydrated as directed, together with 25ml of defibrinated blood (SR0050/SR0051). Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C away from light.

Oxoid Yeast Autolysate Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

Yeast Autolysate Supplement, Oxoid Composition

Yeast Autolysate Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053. Mix gently and pour into sterile Petri dishes.

This product has been discontinued. Please contact us for more information.

Oxoid VCN Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B
• Each vial supplements 500mL of medium

VCN Selective Supplement, Oxoid Composition

VCN Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Thayer Martin Medium, cooled to 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 plus the rehydrated contents of one vial of Vitox SR0090. Mix gently and pour into sterile Petri dishes.

Oxoid Campylobacter Selective Supplement, Blaser-Wang is used to prepare of Blaser-Wang Selective Medium for the isolation of Campylobacter while inhibiting growth of Candida albicans.

• Add to Columbia Blood Agar Base, Part No. CM0271B
• Each vial supplements 500mL of medium

Campylobacter Selective Supplement, Blaser-Wang, Oxoid Composition

Campylobacter Selective Medium, Oxoid Preparation:
Reconstitue one vial as directed add the contents of one vial to 500ml of sterile nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2, with 10% defibrinated horse/sheep blood or 5-7% laked horse blood SR0050, SR0051 or SR0048. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid VCNT Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.

• Add to GC Agar Base, Part No. CM0367B

VCNT Selective Supplement, Oxoid Composition

VCNT Selective Medium Preparation:
Aseptically add 2ml of sterile distilled water to one vial mix gently to dissolve the powder completely. Aseptically add the contents of one vial to 500ml of sterile Thayer Martin Medium, cooled to approximately 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 and the rehydrated contents of one vial of Vitox SR0090.

Oxoid Vitox Supplement is used for the preparation of Thayer Martin Medium and New York City Medium for the isolation of Neisseria spp.

• Contents: Vitox Supplement (5 vials) plus reconstitution fluid (5 vials)
• Add to GC Agar Base, Part No. CM0367B

Ordering Information:

• Each vial of Vitox plus reconstitution fluid supplements 500mL of medium

Includes:

• Five vials of Vitox plus five vials of reconstitution fluid

Vitox Supplement, Oxoid Composition

Vitox Rehydration Fluid , Oxoid Composition

Vitox, Oxoid Preparation:
Aseptically add one vial of Hydration Fluid to one vial of Vitox. Mix gently to dissolve completely. Aseptically add the contents of one vial to sterile medium of choice, cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Oxoid Perfringens OPSP Selective Supplement B is for selective isolation of Clostridium perfringens from food.

• Add to Perfringens Agar Base, OPSP, Part No. CM0543B
• For 500mL of medium

Perfringens OPSP Selective Supplement B, Oxoid Composition

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Perfringens OPSP Selective Supplement A is for selective isolation of Clostridium perfringens from food.

• Add to Perfringens Agar Base, OPSP, Part No. CM0543B
• For 500mL of medium

Perfringens OPSP Selective Supplement A, Oxoid Composition

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Oxoid Dermasel Selective Supplement is used for Dermasel Medium, Part No. CM0539B.

• Each vial supplements 500mL of medium

Dermasel Selective Supplement, Oxoid Composition

Dermasel Agar, Oxoid Preparation:
Suspend 44.5g in 1 litre of distilled water and heat gently to dissolve completely. Add the contents of 1 vial of Dermasel Selective Supplement SR0075, reconstituted as directed in the product insert, to each 500ml of medium to give a level of cycloheximide 0.4g/l and chloramphenicol 0.05g/l. Mix gently and sterilise by autoclaving at 121°C for 10 minutes. Avoid overheating at any time.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
This medium should not be used if agents causing systemic mycoses are being sought⁹. If such agents are suspected e.g. cryptococcus, histoplasma etc., either Dermasel Agar Base without antibiotic supplement must be used in parallel or Brain Heart Infusion Agar CM0375.
If the fungal agent sought is suspected to be nutritionally fastidious, the use of Brain Heart Infusion Agar is particularly helpful.
Dermasel Selective Supplement SR0075 contains a toxic concentration of cycloheximide. Note the precautions to be taken under HAZARDS.

This product has been discontinued. Please contact us for more information.

Oxoid Tinsdale Supplement is used for the cultivation of Corynebacterium diphtheriae.

• A supplement for Tinsdale Medium, Part No. CM0487B
• Each vial supplements 200mL of medium

Tinsdale Supplement, Oxoid Composition

Tinsdale Agar, Oxoid Preparation:
Suspend 9g of agar base in 200ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to approximately 50°C and add the contents of one vial of Oxoid Tinsdale Supplement SR0065 reconstiuted as directed. Mix throughly and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 4 days.

Precautions
Further tests must be carried out on colonies suspected as Corynebacterium diphtheriae, including cell morphology after sub-culture to Loeffler’s Medium and examination for toxin production.
Do not incubate Tinsdale’s Agar plates in enhanced CO2 atmosphere (5-10% v/v).

**This product is not available at the moment. Please contact us for more information.

Thermo Scientific™ Oxoid Horse Serum is an extract of horse blood for addition to culture media.